Isolation of an evolutionarily conserved epidermal growth factor receptor cDNA from human A431 carcinoma cells.

abstract：:In the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were ...

journal_title：Biochemical and biophysical research communications

authors： Fujita R,Hino M,Ebihara T,Nagasato T,Masuda A,Lee JM,Fujii T,Mon H,Kakino K,Nagai R,Tanaka M,Tonooka Y,Moriyama T,Kusakabe T

更新日期：2020-08-20 00:00:00

abstract：:Intranuclear inclusions have been observed in the brains of patients affected with Huntington's disease (HD). Neuro 2A cells that transiently expressed HD exon 1 bearing 74 glutamine repeats linked to the green fluorescent protein (GFP) and the nuclear localization sequence (NLS) contained aggregates in nuclei. The ag...

journal_title：Biochemical and biophysical research communications

authors： Hazeki N,Tsukamoto T,Yazawa I,Koyama M,Hattori S,Someki I,Iwatsubo T,Nakamura K,Goto J,Kanazawa I

更新日期：2002-06-07 00:00:00

abstract：:Previous work had suggested the presence of significant levels of UDP-glucuronosyltransferase 1A1 (UGT1A1) catalytic activity in human small intestinal microsomes, with undetectable to low UGT1A6 and 2B7 activities. To confirm the presence of UGT1A1 isoform in human small bowel, to explore the possible absence of UGT1...

journal_title：Biochemical and biophysical research communications

authors： Paine MF,Fisher MB

更新日期：2000-07-14 00:00:00

abstract：:Angiogenin is a potent angiogenic factor secreted by cultured tumor cells and is found in various normal organs and tissues. The ovary is one of the adult organs in which angiogenesis normally occurs during the female reproductive cycle. In this study, we examined whether angiogenin protein is localized and if angioge...

journal_title：Biochemical and biophysical research communications

authors： Lee HS,Lee IS,Kang TC,Jeong GB,Chang SI

更新日期：1999-04-02 00:00:00

abstract：:We previously reported that fucoidan, a dietary fiber purified from seaweed, inhibited IgE production by B cells in vitro. In this study, we examined the effect of fucoidan on IgE production in vivo. The OVA-induced increase of plasma IgE was significantly suppressed when fucoidan was intraperitoneally, but not orally...

journal_title：Biochemical and biophysical research communications

authors： Yanase Y,Hiragun T,Uchida K,Ishii K,Oomizu S,Suzuki H,Mihara S,Iwamoto K,Matsuo H,Onishi N,Kameyoshi Y,Hide M

更新日期：2009-09-25 00:00:00

abstract：:We investigated the contributions of dietary fat and dietary carbohydrate to the development of fatty liver induced by western diet (WD). Compared with WD-fed wild type (WT) mice, livers of WD-fed ChREBP(-/-) mice showed lipid droplets of varying sizes around the hepatic lobules, while hepatic triglyceride and cholest...

journal_title：Biochemical and biophysical research communications

authors： Wu W,Tsuchida H,Kato T,Niwa H,Horikawa Y,Takeda J,Iizuka K

更新日期：2015-06-12 00:00:00

abstract：:Sarafotoxins (SRTXs) are endothelin-like peptides extracted from snake venom. SRTXs stimulate the endothelin ETA and ETB receptors and enhance vasoconstriction, followed by left ventricular dysfunction and bronchoconstriction. SRTXs include four major isopeptides, S6a-d, with different subtype selectivities. Here, we ...

journal_title：Biochemical and biophysical research communications

authors： Izume T,Miyauchi H,Shihoya W,Nureki O

更新日期：2020-07-23 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) [1,25(OH)(2) D(3)] exerts its biological effects by binding to the vitamin D receptor (VDR), which binds in turn to the vitamin D response elements located in the target gene's promoter. We have previously demonstrated that VDR binds in vitro with high affinity to the 5'-flanking sequence of...

journal_title：Biochemical and biophysical research communications

authors： Seoane S,Alonso M,Segura C,Pérez-Fernández R

更新日期：2002-03-22 00:00:00

abstract：:Asthma is characterized by Th2-mediated inflammation, resulting in airway hyperresponsiveness (AHR) through airway remodeling. Recent epidemiological and experimental reports have suggested an inverse relationship between the development of allergy and helminth infections. Infection by Clonorchis sinensis, a liver flu...

journal_title：Biochemical and biophysical research communications

authors： Jeong YI,Kim SH,Ju JW,Cho SH,Lee WJ,Park JW,Park YM,Lee SE

更新日期：2011-04-22 00:00:00

abstract：:The purpose of this study was to examine whether mechanical tensile forces affect estrogen regulation of collagen synthesis of anterior cruciate ligament fibroblasts at the mRNA level. Estrogen was studied at three physiologic levels, 10(-11), 10(-10), and 10(-9)M. The results revealed that estrogen alone stimulated T...

journal_title：Biochemical and biophysical research communications

authors： Lee CY,Smith CL,Zhang X,Hsu HC,Wang DY,Luo ZP

更新日期：2004-05-14 00:00:00

abstract：:In the bone matrix, insulin-like growth factors (IGFs) are the most abundant growth factors and IGF binding protein 5 (IGFBP-5) is the major IGFBP. Our previous study suggested that IGFs stored in the bone matrix and prostate-specific antigen (PSA) play an important role in prostate cancer (PC) bone metastasis. Howeve...

journal_title：Biochemical and biophysical research communications

authors： Maeda H,Yonou H,Yano K,Ishii G,Saito S,Ochiai A

更新日期：2009-04-10 00:00:00

abstract：:Effects of temperature and d-cis-diltiazem (DTZ) on [3H]nitrendipine (NTD) and [3H]nimodipine (NIM) binding to skeletal muscle t-tubular membranes were studied. A decrease in temperature from 37 degrees C to 10 degrees C decreased KD and increased Bmax slightly. DTZ increased binding by increasing Bmax under all condi...

journal_title：Biochemical and biophysical research communications

authors： Kirley TL,Schwartz A

更新日期：1984-08-30 00:00:00

abstract：:The increasingly appreciated role of astrocytes in neurophysiology dictates a thorough understanding of the mechanisms underlying the communication between astrocytes and neurons. In particular, the uptake and release of signaling substances into/from astrocytes is considered as crucial. The release of different gliot...

journal_title：Biochemical and biophysical research communications

authors： Potokar M,Kreft M,Lee SY,Takano H,Haydon PG,Zorec R

更新日期：2009-12-25 00:00:00

abstract：:We investigated the molecular mechanism underlying the neuroprotective effect of theanine, a green tea component, using primary cultured rat cortical neurons, focusing on group I metabotropic glutamate receptors (mGluRs). Theanine and a group I mGluR agonist, DHPG, inhibited the delayed death of neurons caused by brie...

journal_title：Biochemical and biophysical research communications

authors： Nagasawa K,Aoki H,Yasuda E,Nagai K,Shimohama S,Fujimoto S

更新日期：2004-07-16 00:00:00

abstract：:miRNA is increasingly being recognized as a key regulator of metabolism in animals. A wealth of evidence has suggested that miRNA mainly binds 3' UTR of mRNA and modulates the cell activities via repressing the mRNA translation. However, as the translation initiates at 5' UTR, cis elements like upstream open reading f...

journal_title：Biochemical and biophysical research communications

authors： Hu W,Wang T,Yang Y,Zheng S

更新日期：2014-09-12 00:00:00

abstract：:The spectrum of resting cytochrome c oxidase is modulated by valinomycin addition, which induces a red shift of the Soret band. The enzyme is known both to fluoresce and phosphorescence, effects which can be modulated by certain protein reagents and quenchers. Valinomycin had little effect upon fluorescence at 335 nm,...

journal_title：Biochemical and biophysical research communications

authors： Butko P,He J,Nicholls P

更新日期：1992-12-30 00:00:00

abstract：:Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the long duration of the measurement and limited digital resolution. Recently, new acquisition techniques for speeding up multidimensional NMR experiments, such as nonlinear sampling, i...

journal_title：Biochemical and biophysical research communications

authors： Shigemitsu Y,Ikeya T,Yamamoto A,Tsuchie Y,Mishima M,Smith BO,Güntert P,Ito Y

更新日期：2015-02-06 00:00:00

abstract：:We investigated the influence of pregnancy on the expression of insulin-regulated glucose transporter (GLUT4) mRNA and protein in skeletal muscle and adipose tissue. GLUT4 mRNA expression in quadriceps muscle from control and pregnant rats was similar in the fasted and fed states. When the level of expression was dete...

journal_title：Biochemical and biophysical research communications

authors： Okuno S,Maeda Y,Yamaguchi Y,Takao Y,Trocino RA,Takino H,Kawasaki E,Yokota A,Uotani S,Akazawa S

更新日期：1993-03-15 00:00:00

abstract：:The cell adhesion molecule integrin alphaMbeta2 associates with the urokinase-type plasminogen activator receptor (uPAR) on monocytes and neutrophils. uPAR also associates with members of the beta1 and beta3 integrins, and it modulates the ligand-binding function of these integrins. In this study, we showed that co-ex...

journal_title：Biochemical and biophysical research communications

authors： Tang ML,Kong LS,Law SK,Tan SM

更新日期：2006-09-29 00:00:00

abstract：:Hyperphosphorylation of extracellular signal-regulated protein kinases-1/2 (ERK1/2) is known to promote cancer cell proliferation. We therefore investigated the constitutive phosphorylation levels of ERK1/2 and the expression of its downstream targets c-Fos, c-Jun, and cyclooxygenase-2 (COX-2) in biopsied human gastri...

journal_title：Biochemical and biophysical research communications

authors： Wu WK,Sung JJ,Yu L,Li ZJ,Chu KM,Cho CH

更新日期：2008-08-22 00:00:00

abstract：:After proliferative activation of rat liver cells in vivo by a partial hepatectomy a decrease of the calmodulin content in the three plasma membrane domains (blood sinusoidal, canalicular and lateral) was observed. At 24 hours after partial hepatectomy calmodulin was found to be 3 fold lower in the sinusoidal and late...

journal_title：Biochemical and biophysical research communications

authors： Pujades C,Bastos R,Enrich C,Bachs O

更新日期：1990-12-31 00:00:00

abstract：:Addition of 5% hexadecane to the diet of rats increased fecal excretion of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) from 14 to 39% of an LD50 dose (60 micrograms/kg) during 10 days after dosing. This enhanced elimination did not result in reduced toxicity. On the contrary, the treatment has increased mortality from ...

journal_title：Biochemical and biophysical research communications

authors： Rozman K

更新日期：1984-12-28 00:00:00

abstract：:During the late stage of homologous recombination in prokaryotes, RuvA binds to the Holliday junction intermediate and executes branch migration in association with RuvB. The RuvA subunits form two distinct complexes with the Holliday junction: complex I with the single RuvA tetramer on one side of the four way juncti...

journal_title：Biochemical and biophysical research communications

authors： Mayanagi K,Fujiwara Y,Miyata T,Morikawa K

更新日期：2008-01-11 00:00:00

abstract：:Arthritis is a common autoimmune disease that is associated with progressive disability, systemic complications and early death. However, the underling mechanisms of arthritis are still unclear. Sirtuins are a NAD(+)-dependent class III deacetylase family, and regulate cellular stress, inflammation, genomic stability,...

journal_title：Biochemical and biophysical research communications

authors： Lin J,Sun B,Jiang C,Hong H,Zheng Y

更新日期：2013-11-29 00:00:00

abstract：:Full-length human p53 protein was examined using tryptophan fluorescence and circular dichroism spectroscopy (CD) to monitor unfolding. No significant alteration in tryptophan fluorescence for the tetrameric protein was detectable over a wide range of either urea or guanidine hydrochloride concentrations, in contrast ...

journal_title：Biochemical and biophysical research communications

authors： Nichols NM,Matthews KS

更新日期：2001-10-19 00:00:00

abstract：:Because of the advantages of induced pluripotent stem cells (iPSCs), iPSCs-derived keratinocyte hold great clinical and research potential in wound repair. Similar to other cell transplantation therapies, the migration ability of iPSCs-derived keratinocyte transplanted into skin is critical to the therapeutic effect. ...

journal_title：Biochemical and biophysical research communications

authors： Wu R,Du D,Bo Y,Zhang M,Zhang L,Yan Y

更新日期：2019-11-26 00:00:00

abstract：:The 3.6 kb HindIII DNA fragment of B. sphaericus 1593M chromosomal DNA bears two genes encoding two polypeptides of 41.9 kDa (protein "42") and 51.4 kDa (protein "51"). DNA fragments carrying only one of these two genes when expressed in E. coli yield products that are inactive towards Culex larvae. The larvicidal act...

journal_title：Biochemical and biophysical research communications

authors： de la Torre F,Bennardo T,Sebo P,Szulmajster J

更新日期：1989-11-15 00:00:00

abstract：:P-selectin (CD62P) is a cell adhesion molecule expressed on stimulated endothelial cells and on activated platelets. It interacts with PSGL-1 (P-selectin glycoprotein ligand-1; CD162) on leukocytes and mediates recruitment of leukocytes during inflammation. P-selectin also binds to several types of cancer cells in vit...

journal_title：Biochemical and biophysical research communications

authors： Li L,Short HJ,Qian KX,Elhammer AP,Geng JG

更新日期：2001-11-02 00:00:00

abstract：:The tumor suppressor p53 is involved in cadmium (Cd)-induced apoptosis and autophagy. However, the regulatory mechanisms of p53 in Cd-induced kidney injury are not well established. Here, we report the role of autophagy in Cd-induced p53 induction in human proximal tubular cells (HK-2). HK-2 cells treated with Cd indu...

journal_title：Biochemical and biophysical research communications

authors： Lee HY,Oh SH

更新日期：2021-01-01 00:00:00

abstract：:We proposed to use a novel stepwise sequence-constructive SELEX method to develop DNA aptamers that can recognize Globo H which is a tumor-associated carbohydrate antigen. A combinatorial synthetic library that consisted of DNA molecules with randomized regions of 15-bases was used as the starting library for the firs...

journal_title：Biochemical and biophysical research communications

authors： Wang CY,Wu CY,Hung TC,Wong CH,Chen CH

更新日期：2014-09-26 00:00:00