Crystal structure of human endothelin ETB receptor in complex with sarafotoxin S6b.

abstract：:Eight amino acid permease genes from the protozoan parasite Leishmania donovani (AAPLDs) were cloned, sequenced, and shown to be expressed in promastigotes. Seven of these belong to the amino acid transporter-1 and one to the amino acid polyamino-choline superfamilies. Using these sequences as well as known and charac...

journal_title：Biochemical and biophysical research communications

authors： Akerman M,Shaked-Mishan P,Mazareb S,Volpin H,Zilberstein D

更新日期：2004-12-03 00:00:00

abstract：:We find that the low frequency Raman spectrum of Zn(II) metallothionein has a single prominent band at 138 cm-1 which is absent from the Raman spectrum of the metal-free protein. This feature is also found for Cd(II) binding to both of the independent metallothionein domains and the metallothionein from Neurospora cra...

journal_title：Biochemical and biophysical research communications

authors： Elgren TE,Wilcox DE

更新日期：1989-09-15 00:00:00

abstract：:Oleoyl coenzyme A and other acyl-CoA derivatives inhibited ADP or thrombin-induced aggregation of platelets. Arachidonic acid-induced aggregation was also inhibited, but not the slower aggregation caused by 1-oleoyl-2-acetylglycerol or tetradecanoyl-phorbol-13-acetate. Coenzyme A and free fatty acids had little or no ...

journal_title：Biochemical and biophysical research communications

authors： Lascu I,Edwards B,Cucuianu MP,Deamer DW

更新日期：1988-10-31 00:00:00

abstract：:A secondary structure has been predicted for the hemorrhagic metalloproteases using a method developed in Zurich that extracts structural information from patterns of conservation and variation in homologous protein sequences. This prediction tests the limits of the method when applied to a small number of homologous ...

journal_title：Biochemical and biophysical research communications

authors： Gerloff DL,Jenny TF,Knecht LJ,Benner SA

更新日期：1993-07-15 00:00:00

abstract：:miR-199b-5p is up-regulated significantly during the osteogenesis process in human bone marrow stromal cells (BMSCs). Inhibiting miR-199b-5p notably reduces while over-expressing miR-199b-5p promotes the BMSCs osteoblast differentiation, suggested by the alternations of osteogenic genes expression, ALP activity and th...

journal_title：Biochemical and biophysical research communications

authors： Zhao R,Li Y,Lin Z,Wan J,Xu C,Zeng Y,Zhu Y

更新日期：2016-09-02 00:00:00

abstract：:It has long been assumed that Ca2+ are translocated from the cytosol to the cell nucleus by a long distance to activate transcription machinery buried deep in the nucleoplasm. However, this model has been recently challenged. When HeLa cells were loaded with fluo-3, highly fluorescent spots of approximately 2 microns ...

journal_title：Biochemical and biophysical research communications

authors： Lui PP,Kong SK,Kwok TT,Lee CY

更新日期：1998-06-09 00:00:00

abstract：:The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro kinase assay demonstrated that it is a substrate for MAP kinase. Phosphorylation of endogenous HABP1 was also observed following treatment of J774 cells with PMA. HABP1 was coimmunoprecipitated with activated ERK, confirmi...

journal_title：Biochemical and biophysical research communications

authors： Majumdar M,Meenakshi J,Goswami SK,Datta K

更新日期：2002-03-08 00:00:00

abstract：:A nonreducible cyclic analog of somatostatin (SRIF) was prepared by a combination of solid phase and solution peptide synthesis. The compound, gamma-Abu-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Asp-OH, was tested for its effect on the release of growth hormone, glucagon and insulin in rats. It significantly suppressed ...

journal_title：Biochemical and biophysical research communications

authors： Garsky VM,Clark DE,Grant NH

更新日期：1976-12-20 00:00:00

abstract：:A single base change resulting in the substitution of Cys for Arg at position 519 of the type II collagen triple helix is a predisposing factor in the pathogenesis of a precocious-onset form of familial osteoarthritis associated with a mild chondrodysplasia. Cartilage obtained at the time of total knee replacement in ...

journal_title：Biochemical and biophysical research communications

authors： Holderbaum D,Malemud CJ,Moskowitz RW,Haqqi TM

更新日期：1993-05-14 00:00:00

abstract：:Previous structural and mutational studies of bovine pancreatic deoxyribonuclease I (bpDNase I) have demonstrated that the active site His134 and His252 played critical roles in catalysis. In our present study, mutations of these two His residues to Gln, Ala or Gly reduced the DNase activity by a factor of four to fiv...

journal_title：Biochemical and biophysical research communications

authors： Chen WJ,Lai PJ,Lai YS,Huang PT,Lin CC,Liao TH

更新日期：2007-01-19 00:00:00

abstract：:The acute effects of 1,25-Dihydroxy-vitamin D3 [1,25(OH)2D3] on the concentration of cytoplasmic ionized calcium [Ca2+] of cultured rat mesangial cells were studied at the single cell level by microspectrofluorometry of fura-2-loaded cells. Addition of 1,25(OH)2D3 produced an immediate increase of [Ca2]+. This rise in...

journal_title：Biochemical and biophysical research communications

authors： Boland RL,de Boland AR,Nobiling R,Weinreich T,Ritz E

更新日期：1991-10-31 00:00:00

abstract：:The gut epithelium is critically involved in maintaining intestinal immune homeostasis. Acting as a physical barrier, it separates the intestinal microflora from cells of the immune system. In addition to its barrier function, the intestinal epithelium expresses defensins, natural, endogenous antimicrobial peptides. I...

journal_title：Biochemical and biophysical research communications

authors： Zhao A,Lu W,de Leeuw E

更新日期：2015-11-27 00:00:00

abstract：:A protein, with a novel N-terminal amino acid sequence and a molecular mass of 30 kDa, was purified from fresh Smilax glabra rhizomes by adsorption on DEAE-cellulose, CM-cellulose, Con A-Sepharose, and Mono S, and by fast protein liquid chromatography-gel filtration on Superdex 75. The protein, designated as smilaxin,...

journal_title：Biochemical and biophysical research communications

authors： Chu KT,Ng TB

更新日期：2006-02-03 00:00:00

abstract：:Two genomic DNA clones encoding part of the mouse epidermal growth factor (EGF) receptor were isolated: an 8.0 kb insert that hybridized with the human EGF receptor kinase domain; and a 2.4 kb insert that mapped between the kinase and 3' autophosphorylation domains of the human EGF receptor gene. The 2.4 kb insert was...

journal_title：Biochemical and biophysical research communications

authors： Hung MC,Thompson KL,Chiu IM,Rosner MR

更新日期：1986-12-30 00:00:00

abstract：:Through the proteomic analysis using 2-dimensional electrophoresis, the nicotine addiction-associated proteins were extensively screened in the striatum of rat brains. The nicotine addiction was developed by repeated nicotine injection (0.4mg/kg s.c.), twice daily for 7 days, followed by one challenge injection after ...

journal_title：Biochemical and biophysical research communications

authors： Yeom M,Shim I,Lee HJ,Hahm DH

更新日期：2005-01-14 00:00:00

abstract：:Nikkomycins act as a competitive inhibitor of chitin synthetase and display potent activities against phytopathogenic and human pathogenic fungi. sanT is located in the gene cluster of nikkomycin biosynthesis in Streptomyces ansochromogenes. Sequence analysis revealed that the deduced product of sanT has an unusual do...

journal_title：Biochemical and biophysical research communications

authors： Jia L,Tian Y,Tan H

更新日期：2007-11-03 00:00:00

abstract：:Recently, the concern that toluene might have carcinogenic and reproductive toxic potential has been raised. We investigated the ability of DNA damage by minor metabolites of toluene, methylhydroquinone, and methylbenzoquinone, using (32)P-5'-end-labeled DNA fragments obtained from the human genes. Methylhydroquinone ...

journal_title：Biochemical and biophysical research communications

authors： Murata M,Tsujikawa M,Kawanishi S

更新日期：1999-08-02 00:00:00

abstract：:Discoidin domain receptor 2 (DDR2) is a receptor tyrosine kinase that is activated by fibrillar collagens. DDR2 regulates cell proliferation, cell adhesion, migration, and extracellular matrix remodeling. The decrement of endogenous DDR2 represses osteoblastic marker gene expression and osteogenic differentiation in m...

journal_title：Biochemical and biophysical research communications

authors： Kawai I,Hisaki T,Sugiura K,Naito K,Kano K

更新日期：2012-10-26 00:00:00

abstract：:Heparan sulfate proteoglycans (HSPGs) promote cellular proliferation through interaction with FGF-2. To examine the role of cellular specificity of HSPG in FGF-2 function, a recombinant soluble isoform of CD44 (rsCD44v3,8-10) was expressed in various cell types; 293 T fibroblasts, the epithelial carcinoma cell lines A...

journal_title：Biochemical and biophysical research communications

authors： Fiore MM

更新日期：2001-06-08 00:00:00

abstract：:The solubilized [3H]ryanodine receptor from cardiac sarcoplasmic reticulum was centrifuged through linear sucrose gradients. A single peak of radioactivity with apparent sedimentation coefficient of approximately 30S specifically comigrated with a high molecular weight protein of apparent relative molecular mass appro...

journal_title：Biochemical and biophysical research communications

authors： Lai FA,Anderson K,Rousseau E,Liu QY,Meissner G

更新日期：1988-02-29 00:00:00

abstract：:Highly purified rat brain angiotensin-converting enzyme hydrolyzes substance P which contains a C-terminal amino acid with an amidated carboxyl group. The hydrolysis of substance P verified by amino-group fluorometry and by high-performance liquid chromatography is inhibited by captopril, but not by phosphoramidon. Th...

journal_title：Biochemical and biophysical research communications

authors： Yokosawa H,Endo S,Ogura Y,Ishii S

更新日期：1983-10-31 00:00:00

abstract：:Polycyclic aromatic hydrocarbons (PAHs), aryl hydrocarbon receptor (AHR) ligands, induce atherogenesis. Liver X receptor (LXR) alpha is known to be involved in the control of cholesterol homeostasis. Thus, the purpose of this study was to investigate the effects of 3-methlycholanthrene (MC), one of the PAHs, on LXRalp...

journal_title：Biochemical and biophysical research communications

authors： Iwano S,Nukaya M,Saito T,Asanuma F,Kamataki T

更新日期：2005-09-16 00:00:00

abstract：:Dexamethasone (Dex) causes osteoblast cell injuries. In the present research, we tested the potential effect of SC79, a novel and specific Akt activator, against Dex in osteoblasts. In primary murine osteoblasts and osteoblastic MC3T3-E1 cells, pretreatment with SC79 significantly attenuated Dex-induced cell death. Fu...

journal_title：Biochemical and biophysical research communications

authors： Li ST,Chen NN,Qiao YB,Zhu WL,Ruan JW,Zhou XZ

更新日期：2016-10-07 00:00:00

abstract：:Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Norther...

journal_title：Biochemical and biophysical research communications

authors： Nakanishi T,Kimura Y,Tamura T,Ichikawa H,Yamaai Y,Sugimoto T,Takigawa M

更新日期：1997-05-08 00:00:00

abstract：:Carp acclimated to 10 degrees C gave 69k, 66k, and 62kDa light meromyosin (LMM) fragments in SDS-PAGE, while fish acclimated to 30 degrees C gave 74k, 69k, 66k, and 62kDa fragments. The microsequence analysis revealed that the 69k and 66kDa components from the 10 degrees C-acclimated carp contained an N-terminal amino...

journal_title：Biochemical and biophysical research communications

authors： Watabe S,Imai J,Nakaya M,Hirayama Y,Okamoto Y,Masaki H,Uozumi T,Hirono I,Aoki T

更新日期：1995-03-08 00:00:00

abstract：:Exposure to alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor of ornithine decarboxylase, inhibited the insulin induced differentiation of L6 myoblast cells. Differentiation was assessed by measuring creatine kinase activity and by determining the percentage of nuclei in myotubes. The levels of...

journal_title：Biochemical and biophysical research communications

authors： Erwin BG,Ewton DZ,Florini JR,Pegg AE

更新日期：1983-08-12 00:00:00

abstract：:An endopeptidase capable of metabolizing a number of neuropeptides and generating [Met5] and [Leu5] enkephalin from enkephalin-containing peptides is secreted by glioma C6 cells. This neutral endopeptidase that is likely to be a thiol protease, has a Mr of 71KDa and is effective only towards oligopeptides. Its specifi...

journal_title：Biochemical and biophysical research communications

authors： Ferro ES,Tambourgi DV,Gobersztejn F,Gomes MD,Sucupira M,Armelin MC,Kipnis TL,Camargo AC

更新日期：1993-02-26 00:00:00

abstract：:PAF (0.2 microM) induced maximal platelet aggregation in human PRP and [3H]-PAF (1-5 nM) binding to platelet membrane preparations had Kd value of 3.8 nM and Bmax of 200 fmoles/mg of protein. Without UV irradiation, a synthetic azido tetrahydrofuran derivative L662,025 was a reversible and competitive PAF-receptor ant...

journal_title：Biochemical and biophysical research communications

authors： Hussaini IM,Shen TY

更新日期：1989-05-30 00:00:00

abstract：:A folded synthetic peptide analog of NDF (NDF5), which has the 52 amino acid EGF-like domain of NDF alpha 2, has been characterized. The folded peptide stimulates tyrosine phosphorylation of Her2, Her3 and Her4 in breast cancer cells and competes with low affinity with full-length NDF alpha 2 for binding to the cells,...

journal_title：Biochemical and biophysical research communications

authors： Kita Y,Mayer J,Zamborelli T,Hara S,Rohde M,Watson E,Koski R,Ratzkin B,Nicolson M

更新日期：1995-05-16 00:00:00

abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) is a gamma-herpesvirus that is frequently associated with the formation of tumors like Kaposi's sarcoma and primary effusion lymphomas. KSHV RTA is required for lytic replication/reactivation in KSHV-infected cells. We developed an inducible RTA expression cell line to se...

journal_title：Biochemical and biophysical research communications

authors： Yada K,Do E,Sakakibara S,Ohsaki E,Ito E,Watanabe S,Ueda K

更新日期：2006-06-23 00:00:00