当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Autophagy-mediated cytoplasmic accumulation of p53 leads to apoptosis through DRAM-BAX in cadmium-exposed human proximal tubular cells.

Autophagy-mediated cytoplasmic accumulation of p53 leads to apoptosis through DRAM-BAX in cadmium-exposed human proximal tubular cells.

Abstract：

:The tumor suppressor p53 is involved in cadmium (Cd)-induced apoptosis and autophagy. However, the regulatory mechanisms of p53 in Cd-induced kidney injury are not well established. Here, we report the role of autophagy in Cd-induced p53 induction in human proximal tubular cells (HK-2). HK-2 cells treated with Cd induced the expression of p53, DNA damage autophagy modulator (DRAM), and Bcl-2-associated X protein (BAX), as well as caused poly [ADP-ribose] polymerase 1 (PARP-1) cleavage. Cd exposure also induced autophagy with the accumulation of monomeric p62 and multiple high molecular weight form (HMW)-p62. The expression levels of p53, p62, microtubule-associated protein 1A/1B-light chain 3 (LC3)-1, and LC3-II were similar in the sense that they increased up to 12 h and then gradually decreased. DRAM and BAX levels began to increase post autophagy induction and continued to increase, indicating that autophagy preceded apoptosis. While the genetic knockdown of p53 downregulated HWM-p62, DRAM, and BAX, the expression levels of these proteins were upregulated by p53 overexpression. The genetic knockdown of p62 downregulated p53, autophagy, DRAM, and BAX. The inhibition of autophagy through pharmacological and genetic knockdown reduced p53 and inhibited Cd-induced apoptosis. Collectively, Cd induces apoptosis through p53-mediated DRAM-BAX signaling, which can be regulated by autophagy.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lee HY,Oh SH

doi

10.1016/j.bbrc.2020.12.019

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

128-133

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(20)32197-5

journal_volume

534

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Deltoid human muscle mtDNA is extensively rearranged in old age subjects.

    abstract：:Extra long PCR analysis of mitochondrial DNA (mtDNA) isolated from skeletal muscle of humans of different ages revealed three phenomena: (i) the amount of normal length mtDNA (16.5 kb) was progressively reduced with age, such that the cells of old age individuals (about 90 years) contained little, or undetectable amou...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6251

    authors： Kovalenko SA,Kopsidas G,Kelso JM,Linnane AW

    更新日期：1997-03-06 00:00:00

  • Leukotrienes and related eicosanoids are produced by frog leukocytes.

    abstract：:The metabolites of inflammatory cells produced by massive infection of the peritoneal cavity of two related European species of frogs, Rana temporaria and Rana arvalis were examined for lipoxygenase-generated products of exogenous arachidonic acid. Cells of Rana temporaria produced large amounts of 5-hydroxyeicosatetr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90274-9

    authors： Green FA,Herman CA,Herman RP,Claesson HE,Hamberg M

    更新日期：1987-01-30 00:00:00

  • Ca2+ -dependent interaction of S100A1 with the sarcoplasmic reticulum Ca2+ -ATPase2a and phospholamban in the human heart.

    abstract：:The Ca(2+)-binding S100A1 protein displays a specific and high expression level in the human myocardium and is considered to be an important regulator of heart contractility. Diminished protein levels detected in dilated cardiomyopathy possibly contribute to impaired Ca(2+) handling and contractility in heart failure....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00987-2

    authors： Kiewitz R,Acklin C,Schäfer BW,Maco B,Uhrík B,Wuytack F,Erne P,Heizmann CW

    更新日期：2003-06-27 00:00:00

  • Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients.

    abstract：:The peptidyl-prolyl isomerase Pin1 is prevalently overexpressed in human cancers and is regarded as a new diagnostic and therapeutic target. Pin1 interacts with several proteins involved in cell cycle events in a phosphorylation-dependent manner. Among them, NIMA (never in mitosis, gene A) was first identified to inte...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.228

    authors： Chen J,Li L,Zhang Y,Yang H,Wei Y,Zhang L,Liu X,Yu L

    更新日期：2006-03-24 00:00:00

  • Autocrine galectin-1 promotes collective cell migration of squamous cell carcinoma cells through up-regulation of distinct integrins.

    abstract：:We found that high galectin-1 (Gal-1) mRNA levels were associated with invasive squamous cell carcinoma (SCC) cells that expressed Snail, an epithelial-to-mesenchymal transition (EMT) regulator. Both Gal-1 overexpression and soluble Gal-1 treatment accelerated invasion and collective cell migration, along with activat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.10.152

    authors： Rizqiawan A,Tobiume K,Okui G,Yamamoto K,Shigeishi H,Ono S,Shimasue H,Takechi M,Higashikawa K,Kamata N

    更新日期：2013-11-29 00:00:00

  • Distance between the basic group of the amino acid residue's side chain in position P1 of trypsin inhibitor CMTI-III and Asp189 in the substrate pocket of trypsin has an essential influence on the inhibitory activity.

    abstract：:Three new analogues of trypsin inhibitor CMTI-III were synthesized by the solid-phase method: [Lys5]-CMTI-III, [Orn5]CMTI-III and [Dab5]CMTI-III. Only one analogue with L-lysine residue in position P1 showed inhibitory activity of the same order of magnitude as did wild CMTI-III. Two remaining analogues were completel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7752

    authors： Jaśkiewicz A,Lesner A,Rózycki J,Rodziewicz S,Rolka K,Ragnarsson U,Kupryszewski G

    更新日期：1997-11-26 00:00:00

  • Hypoxia regulates the cAMP- and Ca2+/calmodulin signaling systems in PC12 cells.

    abstract：:Hypoxic/ischemic trauma is a primary factor in the pathology of various disease states. Yet, very little is known about the molecular mechanisms involved in cellular responses and adaptations to hypoxia. As a means of identifying intracellular signaling systems that are regulated in response to hypoxia, the effects of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7907

    authors： Beitner-Johnson D,Leibold J,Millhorn DE

    更新日期：1998-01-06 00:00:00

  • Tissue-specific trans-activation of renin gene by targeted expression of adenovirus E1A in transgenic mice.

    abstract：:We generated two transgenic mice carrying the adenovirus type 12 E1 region genes under control of the human renin promoter, in which the E1A and E1B genes were expressed predominantly in the kidney. Interestingly, renal transcription of the gene for mouse renin was shown to be elevated at 28 and 40 folds as compared i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1212

    authors： Fukamizu A,Sagara M,Sugiyama F,Yagami K,Murakami K

    更新日期：1994-02-28 00:00:00

  • Macrophage paraoxonase 1 (PON1) binding sites.

    abstract：:Paraoxonase 1 (PON1), an HDL-associated esterase, is known to possess anti-oxidant and anti-atherogenic properties. PON1 was shown to protect macrophages from oxidative stress, to inhibit macrophage cholesterol biosynthesis, and to stimulate HDL-mediated cholesterol efflux from the cells. The aim of the present study ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.08.106

    authors： Efrat M,Aviram M

    更新日期：2008-11-07 00:00:00

  • Refolding parameters for the allosteric homodimeric guanylyl cyclase catalytic core from the atrial natriuretic peptide receptor.

    abstract：:Protein folding continues to be an important biophysical topic in molecular biology. We report the parameters for successfully refolding the guanylyl cyclase core of the ANP receptor, an allosteric homodimeric enzyme. Urea was a better chaotropic solvent than guanidine HCl, and physiological salt concentrations and pH...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1097

    authors： Thorpe DS,Niu S,Morkin E

    更新日期：1996-07-25 00:00:00

  • Tissue inhibitor of metalloproteinases-1 (TIMP-1) binds to the cell surface and translocates to the nucleus of human MCF-7 breast carcinoma cells.

    abstract：:To study cellular and subcellular localization of TIMP-1, we constructed a cDNA which would express a chimeric protein, TIMP-1-EGFP, having the enhanced green fluorescent protein of the jelly fish Aequorea victoria fused to the carboxyl-terminus of TIMP-1. Chinese Hamster Ovary (CHO) cells were stably transfected with...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0408

    authors： Ritter LM,Garfield SH,Thorgeirsson UP

    更新日期：1999-04-13 00:00:00

  • Role of apoptosis in growth and differentiation of proximal tubule cells in primary cultures.

    abstract：:In this study, we assessed the profile of cell proliferation, Na,K-ATPase activity and apoptosis in primary cultures of proximal tubule cells. Freshly isolated proximal tubules exhibited apoptosis very early in culture: floated cells within 6 hr; attached tubule cells within 20 hr. The onset of apoptosis in either att...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0118

    authors： Tang MJ,Cheng YR,Lin HH

    更新日期：1996-01-26 00:00:00

  • Reaction of 5'-p-fluorosulfonylbenzoyladenosine with the catalytic and AMP allosteric sites of microsomal HMG-CoA reductase kinase.

    abstract：:The nucleotide analogue 5-p-fluorosulfonylbenzoyladenosine reacts with rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase kinase, causing a rapid loss of the AMP activation capacity and a slower inactivation of the catalytic activity. The rate constant for loss of AMP activation is eleven times higher (K1 =...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80232-2

    authors： Ferrer A,Caelles C,Hegardt FG

    更新日期：1987-11-13 00:00:00

  • General order parameter based correlation analysis of protein backbone motions between experimental NMR relaxation measurements and molecular dynamics simulations.

    abstract：:Internal backbone dynamic motions are essential for different protein functions and occur on a wide range of time scales, from femtoseconds to seconds. Molecular dynamic (MD) simulations and nuclear magnetic resonance (NMR) spin relaxation measurements are valuable tools to gain access to fast (nanosecond) internal mo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.018

    authors： Liu Q,Shi C,Yu L,Zhang L,Xiong Y,Tian C

    更新日期：2015-02-13 00:00:00

  • The role of nitric oxide in delta-aminolevulinic acid (ALA)-induced photosensitivity of cancerous cells.

    abstract：:Application of delta-aminolevulinic acid (ALA) results in the endogenous accumulation of protoporphyrin IX and is a useful approach in the photodynamic therapy (PDT) of cancers. To investigate the role of nitric oxide (NO) in the specific accumulation of protoporphyrin and ALA-induced PDT of cancerous cells, we transf...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.12.007

    authors： Yamamoto F,Ohgari Y,Yamaki N,Kitajima S,Shimokawa O,Matsui H,Taketani S

    更新日期：2007-02-16 00:00:00

  • Isolation and expression of a guanylate cyclase-coupled heat stable enterotoxin receptor cDNA from a human colonic cell line.

    abstract：:Heat stable enterotoxins (STs) are low molecular-weight peptides secreted by enterotoxigenic bacteria. One type of these enterotoxins (STa) induces intestinal secretion leading to acute diarrhea by binding to a membrane form of guanylate cyclase. We have isolated a cDNA from a human colonic cell line, T84, encoding fo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91736-v

    authors： Singh S,Singh G,Heim JM,Gerzer R

    更新日期：1991-09-30 00:00:00

  • KMT Set7/9 is a new regulator of Sam68 STAR-protein.

    abstract：:Lysine-specific methyltransferase Set7/9 (KMT7) belongs to the SET domain family of proteins. Besides the SET domain, Set7/9 also contains a so-called MORN (Membrane Occupation and Recognition Nexus) domain whose function in high eukaryotes is largely unknown. Set7/9 has been shown to specifically methylate both histo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.03.017

    authors： Vasileva E,Shuvalov O,Petukhov A,Fedorova O,Daks A,Nader R,Barlev N

    更新日期：2020-05-14 00:00:00

  • Evaluation of boar sperm as a model system to study the mechanism of spermicidal activity of vanadocenes.

    abstract：:bis-cyclopentadienyl [Cp] complexes of vanadium(IV) or vanadocenes are rapid and potent inhibitors of human sperm motility with potential as a new class of contraceptive agents. We investigated the utility of boar sperm as a model system to study the mechanisms of drug action because boar sperm lacks phosphocreatine a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2517

    authors： D'Cruz OJ,Vassilev A,Uckun FM

    更新日期：2000-04-21 00:00:00

  • Upregulation of PG synthesis on sulfur-starvation for PS I in Chlamydomonas.

    abstract：:Sulfur(S)-starvation was previously shown to induce the degradation of an acidic lipid in chloroplasts, sulfoquinovosyl diacylglycerol (SQDG), to yield a major internal S-source in a green alga, Chlamydomonas reinhardtii. We here found that the synthesis of phosphatidylglycerol (PG), the other acidic lipid in chloropl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.02.058

    authors： Sugimoto K,Midorikawa T,Tsuzuki M,Sato N

    更新日期：2008-05-02 00:00:00

  • Regulation of adipocyte differentiation of bone marrow stromal cells by transcription factor GATA-2.

    abstract：:GATA-2 is a transcription factor expressed in stem and progenitor cells of various cell lineages. In this study, we examined the role of GATA-2 in the differentiation of adipocytes, which are the main components of the microenvironment for hematopoiesis. Suppression of GATA-2 in TBR343, a preadipocytic stromal cell li...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.031

    authors： Okitsu Y,Takahashi S,Minegishi N,Kameoka J,Kaku M,Yamamoto M,Sasaki T,Harigae H

    更新日期：2007-12-14 00:00:00

  • Suppressive effect of transforming growth factor-beta on the phosphorylation of endogenous substrates by conventional and novel protein kinase C in primary cultured mouse epidermal cells.

    abstract：:The effect of transforming growth factor-beta (TGF-beta) on the endogenous protein phosphorylation caused by phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC), was examined in primary cultured mouse epidermal cells. PMA markedly stimulates phosphorylation of endogenous proteins, i.e. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1635

    authors： Nishikawa K,Yamamoto S,Nagumo H,Otsuka C,Kato R

    更新日期：1993-05-28 00:00:00

  • 1-0-Hexadecyl-2-acetyl-sn-glycerol stimulates differentiation of HL-60 human promyelocytic leukemia cells to macrophage-like cells.

    abstract：:The human promyelocytic leukemia cell line HL-60 can be differentiated to cells resembling either neutrophils or mononuclear phagocytes by a diverse group of stimuli. However, the underlying mechanisms remain unknown. We report that 1-0-hexadecyl-2-acetyl-sn-glycerol inhibits the growth of HL-60 cells and induces diff...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80108-4

    authors： McNamara MJ,Schmitt JD,Wykle RL,Daniel LW

    更新日期：1984-07-31 00:00:00

  • Nanosecond electric pulses trigger actin responses in plant cells.

    abstract：:We have analyzed the cellular effects of nanosecond pulsed electrical fields on plant cells using fluorescently tagged marker lines in the tobacco cell line BY-2 and confocal laser scanning microscopy. We observe a disintegration of the cytoskeleton in the cell cortex, followed by contraction of actin filaments toward...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.07.072

    authors： Berghöfer T,Eing C,Flickinger B,Hohenberger P,Wegner LH,Frey W,Nick P

    更新日期：2009-09-25 00:00:00

  • Resveratrol restores the circadian rhythmic disorder of lipid metabolism induced by high-fat diet in mice.

    abstract：:Circadian rhythmic disorders induced by high-fat diet are associated with metabolic diseases. Resveratrol could improve metabolic disorder, but few reports focused on its effects on circadian rhythm disorders in a variety of studies. The aim of the present study was to analyze the potential effects of resveratrol on h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.072

    authors： Sun L,Wang Y,Song Y,Cheng XR,Xia S,Rahman MR,Shi Y,Le G

    更新日期：2015-02-27 00:00:00

  • Gene organization and 5'-flanking region sequence of conglutinin: a C-type mammalian lectin containing a collagen-like domain.

    abstract：:Bovine conglutinin is a Ca(2+)-dependent serum lectin specific for N-acetylglucosamine. In the present study, we report the exon-intron organization and the sequence of 5'-regulatory region of the conglutinin gene. The coding region of conglutinin mRNA, which encoded the signal peptide and the sequence of the mature p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1087

    authors： Kawasaki N,Itoh N,Kawasaki T

    更新日期：1994-01-28 00:00:00

  • Melanoma cell lines express VEGF receptor KDR and respond to exogenously added VEGF.

    abstract：:Tumour-secreted vascular endothelial growth factor (VEGF) exerts a number of effects which are important in tumour pathology, including stimulation of angiogenesis and permeabilisation of tumour-associated vasculature. In this study we have examined the possibility that VEGF may also play an autocrine role in tumour g...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2832

    authors： Liu B,Earl HM,Baban D,Shoaibi M,Fabra A,Kerr DJ,Seymour LW

    更新日期：1995-12-26 00:00:00

  • Decarbamoylating activity of ornithine transcarbamoylase.

    abstract：:We have purified from beef liver an enzyme which decarbamoylates carbamoyl-hemoglobin and to a much lesser extent carbamoyl histones. Carbamoyl casein was a poor substrate while carbamoyl trypsin, fibrinogen and ovoalbumin were not affected. The optimal pH is 7.4. Addition of Mg++, Mn++ or Ca++ was without effect. On ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91698-5

    authors： Costell M,Grisolía S

    更新日期：1985-04-16 00:00:00

  • Mapping studies of an X-linked familial exudative vitreoretinopathy.

    abstract：:Familial exudative vitreoretinopathy (FEVR) is a congenital hereditary, bilateral eye disorder which affects both retina and the vitreous body. As a first step toward the identification of the gene responsible for the X-linked disorder, we report here the results of DNA analyses from the patients and their parents of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1666

    authors： Shastry BS,Trese MT

    更新日期：1993-06-15 00:00:00

  • Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family.

    abstract：:We report the characterization of a new member of the low-density lipoprotein receptor (LDLR) gene family designated LRP10. Human LRP10 cDNA encodes a 1905 amino acid type I membrane protein consisting of five functional domains characteristic of the LDLR gene family. CHO-ldlA7 cells transfected with human LRP10 cDNA ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.12.033

    authors： Jeong YH,Ishikawa K,Someya Y,Hosoda A,Yoshimi T,Yokoyama C,Kiryu-Seo S,Kang MJ,Tchibana T,Kiyama H,Fukumura T,Kim DH,Saeki S

    更新日期：2010-01-01 00:00:00

  • S1P(2) receptors mediate inhibition of glioma cell migration through Rho signaling pathways independent of PTEN.

    abstract：:Sphingosine 1-phosphate (S1P) induced the inhibition of glioma cell migration. Here, we characterized the signaling mechanisms involved in the inhibitory action by S1P. In human GNS-3314 glioblastoma cells, the S1P-induced inhibition of cell migration was associated with activation of RhoA and suppression of Rac1. The...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.12.054

    authors： Malchinkhuu E,Sato K,Maehama T,Mogi C,Tomura H,Ishiuchi S,Yoshimoto Y,Kurose H,Okajima F

    更新日期：2008-02-22 00:00:00