Ca2+ -dependent interaction of S100A1 with the sarcoplasmic reticulum Ca2+ -ATPase2a and phospholamban in the human heart.

Abstract:

:The Ca(2+)-binding S100A1 protein displays a specific and high expression level in the human myocardium and is considered to be an important regulator of heart contractility. Diminished protein levels detected in dilated cardiomyopathy possibly contribute to impaired Ca(2+) handling and contractility in heart failure. To elucidate the S100A1 signaling pathway in the human heart, we searched for S100A1 target proteins by applying S100A1-specific affinity chromatography and immunoprecipitation techniques. We detected the formation of a Ca(2+)-dependent complex of S100A1 with SERCA2a and PLB in the human myocardium. Using confocal laser scanning microscopy, we showed that all three proteins co-localize at the level of the SR in primary mouse cardiomyocytes and confirmed these results by immunoelectron microscopy in human biopsies. Our results support a regulatory role of S100A1 in the contraction-relaxation cycle in the human heart.

authors

Kiewitz R,Acklin C,Schäfer BW,Maco B,Uhrík B,Wuytack F,Erne P,Heizmann CW

doi

10.1016/s0006-291x(03)00987-2

subject

Has Abstract

pub_date

2003-06-27 00:00:00

pages

550-7

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X03009872

journal_volume

306

pub_type

杂志文章
  • The role of RHOA signaling in trophectoderm cell-fate decision in cattle.

    abstract::Mammalian blastocysts are composed of two distinct cell lineages, namely the inner cell mass (ICM) and trophectoderm (TE). TE cells that give rise to the embryonic placenta are marked by an exclusive expression of the key determinant transcription factor, CDX2. Although Hippo signaling pathway is known to be responsib...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2020.05.210

    authors: Kohri N,Akizawa H,Iisaka S,Bai H,Takahashi M,Kawahara M

    更新日期:2020-08-06 00:00:00

  • Protein phosphorylation and activation of platelets by wheat germ agglutinin.

    abstract::The incorporation of 32Pi into the 47 and 20 kDa polypeptides in platelets activated by wheat germ agglutinin (WGA) was studied. The pattern of enhanced phosphorylation produced by the lectin was comparable to that by thrombin. The 47 kDa polypeptide was phosphorylated at both serine and threonine while the 20 kDa pro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)91024-1

    authors: Ganguly CL,Chelladurai M,Ganguly P

    更新日期:1985-10-15 00:00:00

  • G protein-gated K+ channel (GIRK1) protein is expressed presynaptically in the paraventricular nucleus of the hypothalamus.

    abstract::We prepared a specific antibody against GIRK1, the major subunit of the G protein-gated K+ (KG) channel. Immunohistochemical study revealed that GIRK1 immunoreactivity was detected in the presynaptic, but not in the postsynaptic, region in the paraventricular nucleus of the rat hypothalamus (PVN). Therefore, activatio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.0400

    authors: Morishige KI,Inanobe A,Takahashi N,Yoshimoto Y,Kurachi H,Miyake A,Tokunaga Y,Maeda T,Kurachi Y

    更新日期:1996-03-18 00:00:00

  • Salivary phospholipid secretion in response to beta-adrenergic stimulation is mediated by Src kinase-dependent epidermal growth factor receptor transactivation.

    abstract::Communication between receptor tyrosine kinase and G protein-coupled receptor (GPCR)-mediated signaling is recognized as a common integrator linking diverse aspects of intracellular signaling systems. Here, we report that G protein-coupled beta-adrenergic receptor activation leading to stimulation of salivary phosphol...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.04.016

    authors: Slomiany BL,Slomiany A

    更新日期:2004-05-21 00:00:00

  • Nuclear-factor-kappaB (NF-kappaB) and radical oxygen species play contrary roles in transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in hepatocellular carcinoma (HCC) cells.

    abstract::Nuclear-Factor-kappaB (NF-kappaBeta can counteract transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in malignant hepatocytes through up-regulation of its downstream genes, such as X-linked inhibitor of apoptosis protein (XIAP). Reports have demonstrated that TGF-beta1 can induce oxidative stress, and c-J...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.10.130

    authors: Wang F,Kaur S,Cavin LG,Arsura M

    更新日期:2008-12-26 00:00:00

  • Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions.

    abstract::The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)(24) and 5'-(dGdC)(18), was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.05.119

    authors: D'Urso A,Choi JK,Shabbir-Hussain M,Ngwa FN,Lambousis MI,Purrello R,Balaz M

    更新日期:2010-06-25 00:00:00

  • Suppression of protein l-isoaspartyl (d-aspartyl) methyltransferase results in hyperactivation of EGF-stimulated MEK-ERK signaling in cultured mammalian cells.

    abstract::l-Aspartyl (l-Asp) and l-asparaginyl residues in proteins isomerize or racemize to d,l-isoaspartyl (d,l-isoAsp) or d-aspartyl (d-Asp) residues during protein aging. These atypical aspartyl residues can interfere with the biological function of the protein and lead to cellular dysfunction. Protein l-isoaspartyl (d-aspa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.03.109

    authors: Kosugi S,Furuchi T,Katane M,Sekine M,Shirasawa T,Homma H

    更新日期:2008-06-20 00:00:00

  • Synthesis and characterization of folate-PEG-grafted-hyperbranched-PEI for tumor-targeted gene delivery.

    abstract::A great challenge for gene therapy is to develop a high efficient gene delivery system with low toxicity. Nonviral vectors are still attractive although the current agents displayed some disadvantages (i.e., low transfection efficiency, high toxicity). To overcome the high toxicity of poly(ethylene imine) (PEI) and lo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.01.024

    authors: Liang B,He ML,Xiao ZP,Li Y,Chan CY,Kung HF,Shuai XT,Peng Y

    更新日期:2008-03-21 00:00:00

  • HGF-induced capillary morphogenesis of endothelial cells is regulated by Src.

    abstract::The signal transduction pathway involved in hepatocyte growth factor (HGF)-induced capillary morphogenesis of endothelial cells was investigated. HGF-induced capillary morphogenesis of the murine spleen endothelial cell line MSS31 was inhibited by a Src family kinase inhibitor, PP2. Stable expression of kinase-inactiv...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.03.183

    authors: Kanda S,Kanetake H,Miyata Y

    更新日期:2006-06-02 00:00:00

  • Regulation of ERK1/2 activity upon contact inhibition in fibroblasts.

    abstract::Contact inhibition is a crucial mechanism regulating proliferation in vitro and in vivo. Despite its generally accepted importance for maintaining tissue homeostasis knowledge about the underlying molecular mechanisms of contact inhibition is still scarce. Since the MAPK ERK1/2 plays a pivotal role in the control of p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.02.080

    authors: Küppers M,Faust D,Linz B,Dietrich C

    更新日期:2011-03-18 00:00:00

  • The HMGB1 acidic tail regulates HMGB1 DNA binding specificity by a unique mechanism.

    abstract::HMGB1 is a conserved chromosomal protein composed of two DNA-binding domains and an acidic C-terminal tail. There were evidences suggesting that the C-terminal tail contributed to the DNA binding specificity of the N-terminal DNA-binding domains. However, the mechanism underlining this observation is largely unknown. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.05.130

    authors: Wang Q,Zeng M,Wang W,Tang J

    更新日期:2007-08-17 00:00:00

  • Reduction of Cripto-1 expression by a hammerhead-shaped RNA molecule results from inhibition of translation rather than mRNA cleavage.

    abstract::Cripto-1 (CR-1) is a transforming growth factor which has been associated with breast, colon, and pancreatic cancer. Overexpression of CR-1 in non-tumorigenic mouse mammary epithelial cells and fibroblasts results in an increase in anchorage-dependent and -independent growth in vitro. Reduction of CR-1 expression in h...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.8517

    authors: Kintner RL,Hosick HL

    更新日期:1998-04-28 00:00:00

  • A metal-dependent form of protein phosphatase 2A.

    abstract::Highly purified bovine heart protein phosphatase 2A catalytic subunit lost virtually all of its activity during storage at -70 degrees. When the enzyme was preincubated with Co2+, over 35% of the original activity was restored. Freshly prepared protein phosphatase 2A purified from bovine heart was stimulated at least ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1995.1334

    authors: Cai L,Chu Y,Wilson SE,Schlender KK

    更新日期:1995-03-08 00:00:00

  • Glucose regulation of CDK7, a putative thiol related gene, in experimental diabetic nephropathy.

    abstract::We previously described the identification of the 3'end of an unknown gene CDK7 using differential display which appeared to be up-regulated in diabetic kidneys [R.A. Page, C.A. Morris, J.D. Williams, C.J. von Ruhland, A.N. Malik, Isolation of diabetes-associated kidney genes using differential display, Biochem. Bioph...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.03.132

    authors: Malik AN,Rossios C,Al-Kafaji G,Shah A,Page RA

    更新日期:2007-05-25 00:00:00

  • SMRT has tissue-specific isoform profiles that include a form containing one CoRNR box.

    abstract::SMRT acts as a corepressor for a range of transcription factors. The amino-terminal part of the protein includes domains that mainly mediate transcriptional repression whilst the carboxy-terminal part includes domains that interact with nuclear receptors using up to three motifs called CoRNR boxes. The region of the S...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.06.175

    authors: Short S,Malartre M,Sharpe C

    更新日期:2005-09-02 00:00:00

  • Differential phosphorylation of paxillin in response to surface-bound serum proteins during early osteoblast adhesion.

    abstract::An early signaling event during the adhesion and spreading of cells is integrin-mediated tyrosine phosphorylation of the cytoskeletal adaptor protein paxillin and the non-receptor tyrosine kinase pp125(FAK) at focal contacts. To determine the influence of surface-charge and -adsorbed adhesion proteins on this signalin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.5198

    authors: Sommerfeldt DW,McLeod KJ,Rubin CT,Hadjiargyrou M

    更新日期:2001-07-13 00:00:00

  • Spectroscopic and voltammetric characterisation of the bacterioferritin-associated ferredoxin of Escherichia coli.

    abstract::The bacterioferritin-associated ferredoxin (Bfd) of Escherichia coli is a 64-residue polypeptide encoded by the bfd gene located upstream of the gene (bfr) encoding the iron-storage haemoprotein, bacterioferritin. The Bfd sequence resembles those of the approximately 60-residue domains found in NifU proteins (required...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.1856

    authors: Quail MA,Jordan P,Grogan JM,Butt JN,Lutz M,Thomson AJ,Andrews SC,Guest JR

    更新日期:1996-12-13 00:00:00

  • Cellular enrichment with polyunsaturated fatty acids induces an oxidative stress and activates the transcription factors AP1 and NFkappaB.

    abstract::A 48-h incubation of cultured human fibroblasts with 5 x 10(-5) M oleic acid or polyunsaturated fatty acids (PUFA) from the (n-6) (linoleic, gamma-linolenic and arachidonic acids) or (n-3) (alpha-linolenic and eicosapentaenoic acids) series resulted in an enrichment of the cells with the introduced fatty acid. Cell en...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1644

    authors: Mazière C,Conte MA,Degonville J,Ali D,Mazière JC

    更新日期:1999-11-01 00:00:00

  • Tyrosine kinase-dependent phosphatidylinostiol turnover and functional responses in the Fc epsilon R1 signalling pathway.

    abstract::In RBL-2H3 rat basophilic leukemia cells, Fc epsilon R1 crosslinking by multivalent antigen stimulates phosphatidylinositol (PI) turnover and Ca2+ influx and causes functional responses that include secretion, membrane ruffling and actin polymerization. Here, we show that the tyrosine kinase inhibitor, genistein, inhi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)91406-3

    authors: Deanin GG,Martinez AM,Pfeiffer JR,Gardner ME,Oliver JM

    更新日期:1991-08-30 00:00:00

  • Generation of neutralizing monoclonal antibodies against Enterovirus 71 using synthetic peptides.

    abstract::Enterovirus 71 (EV71) has led to recent outbreaks of hand, foot and mouth disease (HFMD) in China, resulting in high mortality. In this study, several monoclonal antibodies were generated by immunizing mice with two synthetic peptides, SP55 and SP70, containing amino acids 163-177 and 208-222 of VP1. The specificities...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.09.103

    authors: Li X,Mao C,Ma S,Wang X,Sun Z,Yi Y,Guo M,Shen X,Sun L,Bi S

    更新日期:2009-12-25 00:00:00

  • Metabolism and detoxification of TNT by Phanerochaete chrysosporium.

    abstract::Several lines of evidence suggest that TNT detoxification by Phanerochaete chrysosporium is through reduction. Rates of TNT reduction were directly correlated with mycelial mass and TNT concentration. Toxicity was inversely related to the amount of fungus. TNT toxicity was identical in both ligninolytic and nonlignino...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1440

    authors: Stahl JD,Aust SD

    更新日期:1993-04-30 00:00:00

  • Endothelin action: Inhibition by a protein kinase C inhibitor and involvement of phosphoinositols.

    abstract::Endothelin tightly bound to rabbit aortic strips and caused a prolonged vasoconstriction both in the presence and absence of extracellular Ca2+, although only partial constriction (20-30%) developed in the latter case, indicating that its action may not be limited to the opening of a calcium channel. The endothelin-in...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(89)80193-7

    authors: Sugiura M,Inagami T,Hare GM,Johns JA

    更新日期:1989-01-16 00:00:00

  • Hepatocyte-specific deletion of the keap1 gene activates Nrf2 and confers potent resistance against acute drug toxicity.

    abstract::Nrf2 is a key regulator of many detoxifying enzyme genes, and cytoplasmic protein Keap1 represses the Nrf2 activity under quiescent conditions. Germ line deletion of the keap1 gene results in constitutive activation of Nrf2, but the pups unexpectedly died before weaning. To investigate how constitutive activation of N...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.10.185

    authors: Okawa H,Motohashi H,Kobayashi A,Aburatani H,Kensler TW,Yamamoto M

    更新日期:2006-01-06 00:00:00

  • Beta-1,4-galactosyltransferase and lactose synthase: molecular mechanical devices.

    abstract::Recent structural investigations on the beta-1,4-galactosyltransferase-1 (Gal-T1) and lactose synthase (LS) have revealed that they are akin to an exquisite mechanical device with two well-coordinated flexible loops that are contained within the Gal-T1 catalytic domain. The smaller one has a Trp residue (Trp314) flank...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1006/bbrc.2002.6506

    authors: Ramakrishnan B,Boeggeman E,Qasba PK

    更新日期:2002-03-15 00:00:00

  • A repressor region in the human beta-myosin heavy chain gene that has a partial position dependency.

    abstract::Expression of the human beta-myosin heavy chain (beta MHC) gene was studied by transient assay in culture and in situ by direct injection of plasmids into adult rat hearts. In this report we describe a unique repressor region located -326/-309 (5'-TTGGTGGTCGTGGTCAGT-3') of the human beta MHC gene that is conserved amo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)91586-f

    authors: Edwards JG,Bahl JJ,Flink I,Milavetz J,Goldman S,Morkin E

    更新日期:1992-11-30 00:00:00

  • SDF-1alpha-induced intracellular calcium transient involves Rho GTPase signalling and is required for migration of hematopoietic progenitor cells.

    abstract::Signalling through the chemokine stromal derived factor (SDF)-1alpha and its receptor CXCR4 has been recognized as a key event in the migratory response of hematopoietic stem and progenitor cells (HPC). Small GTPases of the Rho/Rac family might be involved in SDF-1alpha signalling at several different levels. In the p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2003.10.112

    authors: Henschler R,Piiper A,Bistrian R,Möbest D

    更新日期:2003-11-28 00:00:00

  • Analysis of type-I and type-II RUNX2 protein expression in osteoblasts.

    abstract::Runt-related transcription factor-2 (RUNX2) is expressed as two isoforms (type-I and type-II) differing only in their amino terminal sequences. The amino terminus of type-I contains MRIPV instead of MASNSLFSAVTPCQQSFFW in type-II. Although type-II mRNA has been considered osteoblast specific, the RUNX2 protein isoform...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.5363

    authors: Sudhakar S,Katz MS,Elango N

    更新日期:2001-08-10 00:00:00

  • Deficiency of the 15-kDa selenoprotein led to cytoskeleton remodeling and non-apoptotic membrane blebbing through a RhoA/ROCK pathway.

    abstract::The 15-kDa selenoprotein (Sep15) has been implicated in etiology of some types of cancer. Herein, inducible RNAi cell lines were established and cell morphology and motility were analyzed. The majority of Sep15-deficient cells (>95%) formed membrane blebs in a dynamic manner. Blebbing cells transformed cell morphology...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.12.059

    authors: Bang J,Jang M,Huh JH,Na JW,Shim M,Carlson BA,Tobe R,Tsuji PA,Gladyshev VN,Hatfield DL,Lee BJ

    更新日期:2015-01-24 00:00:00

  • Biosynthesis of tetrahydrobiopterin: conversion of dihydroneopterin triphosphate to tetrahydropterin intermediates.

    abstract::It is known that the first step in the de novo synthesis of tetrahydrobiopterin from GTP is the conversion of GTP to dihydroneopterin triphosphate. Recent evidence supports the conclusion that beyond this first step, the pterin intermediates in the pathway are all at the tetrahydro level of reduction. We have now show...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)91053-8

    authors: Milstien S,Kaufman S

    更新日期:1985-05-16 00:00:00

  • Distribution and characterization of immunoreactive prolactin-releasing peptide (PrRP) in rat tissue and plasma.

    abstract::We established a sensitive and specific two-site enzyme immunoassay (EIA) for prolactin-releasing peptide (PrRP) using two region-specific monoclonal antibodies. We investigated the tissue distribution and the plasma concentration of immunoreactive (ir-) PrRP in rats using this assay. Ir-PrRP was widely distributed in...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0463

    authors: Matsumoto H,Murakami Y,Horikoshi Y,Noguchi J,Habata Y,Kitada C,Hinuma S,Onda H,Fujino M

    更新日期:1999-04-13 00:00:00