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SNX1 and SNX2 mediate retrograde transport of Shiga toxin.

Abstract：

:The bacterial toxin Shiga toxin (Stx) is transported retrogradely from early endosomes to the Golgi apparatus on its way to the endoplasmic reticulum (ER) and the cytosol. In this study we explored the functions of the two phosphoinositide binding proteins Sorting nexin 1 (SNX1) and Sorting nexin 2 (SNX2) in endosomal sorting of the toxin. When Vero cells were depleted of either SNX1 or SNX2 by small interfering RNA (siRNA), Stx transport to the trans-Golgi network (TGN) was impaired by > or = 40%, whereas combined depletion of SNX1 and SNX2 gave a total inhibition of approximately 80%. Inhibition of PI(3)P formation by wortmannin resulted in a similar reduction. Thus, although being partly redundant, both SNX1 and SNX2 are required for efficient Stx trafficking to the Golgi apparatus.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Utskarpen A,Slagsvold HH,Dyve AB,Skånland SS,Sandvig K

doi

10.1016/j.bbrc.2007.04.159

subject

Has Abstract

pub_date

2007-06-29 00:00:00

pages

566-70

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)00907-2

journal_volume

358

pub_type

杂志文章

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