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Solubilization of the iron molybdenum cofactor of Azotobacter vinelandii nitrogenase in dimethylformamide and acetonitrile.

Abstract：

:The iron molybdenum cofactor of Azotobacter vinelandii nitrogenase has been solubilized for the first time in dimethylformamide and acetonitrile. These solutions have the ability to reconstitute the inactive nitrogenase of the UW 45 mutant of A. vinelandii and exhibit an S = 3/2 EPR signal similar to that for the cofactor in N-methylformamide. Our ability to obtain solutions of FeMoco in these solvents seemingly refutes a previous hypothesis concerning the necessity of solvents with a dissociable proton for iron molybdenum cofactor solubility and should facilitate the spectroscopic characterization of this important species.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Lough SM,Jacobs DL,Lyons DM,Watt GD,McDonald JW

doi

10.1016/s0006-291x(86)80053-5

subject

Has Abstract

pub_date

1986-09-14 00:00:00

pages

740-6

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(86)80053-5

journal_volume

139

pub_type

杂志文章

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