Endothelin system in intestinal villi: A possible role of endothelin-2/vasoactive intestinal contractor in the maintenance of intestinal architecture.

abstract：:Standardized RT-PCR (StaRT-PCR) enables numerical quantification as well as intra- and inter-laboratory comparison of gene expression. Multiplex StaRT-PCR, using two rounds of amplification, was conducted on Stratagene Universal Reference RNA. In the first round, cDNA, competitive template (CT) mix, and primers for up...

journal_title：Biochemical and biophysical research communications

authors： Crawford EL,Warner KA,Khuder SA,Zahorchak RJ,Willey JC

更新日期：2002-04-26 00:00:00

abstract：:α-Isopropylmalate synthase (α-IPMS) is a multi-domain protein catalysing the condensation of α-ketoisovalerate (α-KIV) and acetyl coenzyme A (AcCoA) to form α-isopropylmalate. This reaction is the first committed step in the leucine biosynthetic pathway in bacteria and plants, and α-IPMS is allosterically regulated by...

journal_title：Biochemical and biophysical research communications

authors： Huisman FH,Squire CJ,Parker EJ

更新日期：2013-04-05 00:00:00

abstract：:A two fold stimulation in the incorporation of [3H-methyl] groups from [3H-methyl] methionine into phospholipids was seen in intact pancreatic islets within six minutes of exposure to a glucose concentration that stimulates insulin release. Nonstimulatory sugars, L-glucose and D-galactose, as well as dibutyryl cAMP, d...

journal_title：Biochemical and biophysical research communications

authors： Kowluru A,Rana RS,MacDonald MJ

更新日期：1984-07-31 00:00:00

abstract：:We have studied the functional properties of an alternately spliced form of sheep testicular FSH receptor cDNA that codes for a protein similar to a previously described active receptor but differs in the carboxy terminus in sequence and is also shorter by 25 residues. The receptor expressed in HEK 293 cells fails to ...

journal_title：Biochemical and biophysical research communications

authors： Sairam MR,Jiang LG,Yarney TA,Khan H

更新日期：1996-09-24 00:00:00

abstract：:GLUT4, the insulin-responsive glucose transporter expressed primarily in muscle and adipose tissue, contains a single N-glycosylation site. We characterized a mutant GLUT4 lacking the N-glycosylation site (Asn57-->Gln) in primary cultures of rat adipose cells. We transiently transfected cells with expression vectors f...

journal_title：Biochemical and biophysical research communications

authors： Ing BL,Chen H,Robinson KA,Buse MG,Quon MJ

更新日期：1996-01-05 00:00:00

abstract：:Cancer stem cells (CSCs) play essential role in the progression of many tumors. Wnt/β-catenin pathway is crucial in maintaining the stemness of CSCs. (-)-Epigallocatechin-3-gallate (EGCG), the major bioactive component in green tea, has been shown to possess anti-cancer activity. To date, the interventional effect of ...

journal_title：Biochemical and biophysical research communications

authors： Zhu J,Jiang Y,Yang X,Wang S,Xie C,Li X,Li Y,Chen Y,Wang X,Meng Y,Zhu M,Wu R,Huang C,Ma X,Geng S,Wu J,Zhong C

更新日期：2017-01-01 00:00:00

abstract：:Osteoclasts play a crucial role in osteolytic bone diseases, such as osteoporosis, rheumatoid arthritis, periodontitis, Paget's disease of bone and bone metastatic tumors. Therefore, controlling osteoclast differentiation and function has been considered a promising therapeutic strategy. Here, we show that necrostatin...

journal_title：Biochemical and biophysical research communications

authors： Fuji H,Ohmae S,Noma N,Takeiri M,Yasutomi H,Izumi K,Ito M,Toyomoto M,Iwaki S,Takemoto K,Seo S,Taura K,Hida S,Aoyama M,Ishihama Y,Hagiwara M,Takeda N,Hatano E,Iwaisako K,Uemoto S,Asagiri M

更新日期：2018-09-05 00:00:00

abstract：:ATP cassette binding protein 1 (ABCA1) controls the apolipoprotein-mediated cholesterol efflux pathway and determines plasma HDL levels. Although cAMP is known to promote ABCA1 expression and cholesterol efflux from cells, it has not been determined whether cyclic nucleotide phosphodiesterase (PDE) isoforms regulate t...

journal_title：Biochemical and biophysical research communications

authors： Lin G,Bornfeldt KE

更新日期：2002-01-18 00:00:00

abstract：:Eicosanoid formation by transcellular routes can amplify the levels and types of lipid mediators within a local milieu. To evaluate the role of adhesion in this process, we assessed the influence of mAb against adhesion molecules on LTC4 generation by PMN-endothelial cell interaction. Transcellular LTC4 generation was...

journal_title：Biochemical and biophysical research communications

authors： Brady HR,Serhan CN

更新日期：1992-08-14 00:00:00

abstract：:A mitochondrial DNA mutation at nucleotide position 14,484 was found in 14 independent probands with Leber hereditary optic neuropathy and in 0/250 controls. The 14,484 mutation, which changes methionine-64 to valine in a conserved domain of the ND-6 gene, occurred in association with a mitochondrial DNA haplotype tha...

journal_title：Biochemical and biophysical research communications

authors： Johns DR,Neufeld MJ,Park RD

更新日期：1992-09-30 00:00:00

abstract：:During retroviral assembly, tRNAs are incorporated into the virion, one of which serves as a primer for the reverse transcription reaction. Using two dimensional polyacrylamide gel electrophoresis, we have studied the patterns of tRNAs incorporated into HIV-1 (3B) produced either in the lymphoid cell line H-9 or in th...

journal_title：Biochemical and biophysical research communications

authors： Kleiman L,Caudry S,Boulerice F,Wainberg MA,Parniak MA

更新日期：1991-02-14 00:00:00

abstract：:We have recently observed that attomolar concentration of exogenously added TGF beta, a molecule structurally related to inhibin, can stimulate the basal secretion of FSH in a pituitary cell culture. Inhibin purified from porcine follicular fluid antagonizes this activity of TGF beta. To understand further the homeost...

journal_title：Biochemical and biophysical research communications

authors： Ying SY,Becker A,Ling N,Ueno N,Guillemin R

更新日期：1986-05-14 00:00:00

abstract：:Isolated rat cerebral cortex cells were able to accumulate L-carnitine and this process was competitively inhibited by 1 mM gamma-aminobutyric acid (GABA) with a shift of Km from 7.8 +/- 1.9 mM to 14.6 +/- 4.0 mM. Addition of GABA also affected distribution of carnitine derivatives. The decrease of acetylcarnitine lev...

journal_title：Biochemical and biophysical research communications

authors： Wawrzeńczyk A,Nałecz KA,Nałecz MJ

更新日期：1995-08-15 00:00:00

abstract：:Farnesoid X receptor (FXR), a bile acid receptor, is known to be involved in the promotion of adipogenesis. However, the regulation mechanism of FXR-promoted adipogenesis remains unclear. In this study, we investigated the regulation mechanism of FXR-mediated activation of adipogenesis in murine adipocyte 3T3-L1 cells...

journal_title：Biochemical and biophysical research communications

authors： Shinohara S,Fujimori K

更新日期：2020-06-18 00:00:00

abstract：:Preeclampsia (PE) is the leading cause of maternal and perinatal mortality and morbidity. Understanding the molecular mechanisms underlying placentation facilitates the development of better intervention of this disease. MicroRNAs are strongly implicated in the pathogenesis of this syndrome. In current study, we found...

journal_title：Biochemical and biophysical research communications

authors： Li Q,Pan Z,Wang X,Gao Z,Ren C,Yang W

更新日期：2014-10-10 00:00:00

abstract：:In the present study we used LLC-PK1 cells, a porcine renal proximal tubular cell line, to investigate whether PI3 kinase activation was involved in the anti-apoptotic effect of ouabain, a specific inhibitor of Na,K-ATPase. Apoptosis was induced by actinomycin D (Act D, 5 microM) and assessed by appearance of hypodipl...

journal_title：Biochemical and biophysical research communications

authors： Zhou X,Jiang G,Zhao A,Bondeva T,Hirszel P,Balla T

更新日期：2001-07-06 00:00:00

abstract：:Leber hereditary optic neuropathy (LHON) was the first disease to be linked to the presence of a mitochondrial DNA (mtDNA) mutation. Nowadays over 95% of LHON cases are known to be caused by one of three primary mutations (m.11778G>A, m.14484T>C, and m.3460G>A). Reports for other (rare) primary mutations in LHON patie...

journal_title：Biochemical and biophysical research communications

authors： Zhang AM,Zou Y,Guo X,Jia X,Zhang Q,Yao YG

更新日期：2009-08-21 00:00:00

abstract：:We have previously shown that estrogen effects in the heart include direct hormone effects on the myocardium. In a recent study we found that one beneficial effect of estradiol on the myocardium is the inhibition of apoptosis in cardiac myocytes. This effect was associated with a reduction of NF-kappaB activity. In th...

journal_title：Biochemical and biophysical research communications

authors： Pelzer T,Neumann M,de Jager T,Jazbutyte V,Neyses L

更新日期：2001-09-07 00:00:00

abstract：:An important role for the Rel/NF-kappaB family of transcription factors in the differentiation process of dendritic cells (DC) and macrophages (MAC) was recently suggested by a number of mouse knockout studies but only little information is available for defined populations of human cells. To investigate the role of i...

journal_title：Biochemical and biophysical research communications

authors： Ammon C,Mondal K,Andreesen R,Krause SW

更新日期：2000-02-05 00:00:00

abstract：:A procedure was developed for real-time measurement of the interaction between an archaeal TATA-binding protein (TBP) with stress-gene promoters from the archaeon Methanosarcina mazeii using surface plasmon resonance (SPR), the BIACORE 3000 equipment, and the SA (streptavidin) Sensor Chip. Measurements were based on t...

journal_title：Biochemical and biophysical research communications

authors： Conway de Macario E,Rudofsky UH,Macario AJ

更新日期：2002-11-15 00:00:00

abstract：:Glial precursor cells (GPCs) are present in the adult human central nervous system (CNS) and they can be isolated and maintained in culture for in vitro studies. This study analysed expression of mGluR3 and mGluR5 metabotropic glutamate receptor (mGluR) mRNAs in GPCs. A2B5 surface antigen positive GPCs were isolated u...

journal_title：Biochemical and biophysical research communications

authors： Luyt K,Varadi A,Halfpenny CA,Scolding NJ,Molnar E

更新日期：2004-06-18 00:00:00

abstract：:Cardiolipin and phosphatidic acid-binding protein (CLPABP) controls the stability of the mRNA harboring an AU-rich element (ARE) in the 3' UTR with the help of the RNA stabilizer, human antigen R (HuR). Although CLPABP is localized on the mitochondrial surface as a large protein-RNA complex, its precise role is not ye...

journal_title：Biochemical and biophysical research communications

authors： Maeda A,Uchida M,Nishikawa S,Nishino T,Konishi H

更新日期：2018-01-01 00:00:00

abstract：:Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as ''dedifferentiation'' or ''transdifferentiation''. However, since MSCs have only b...

journal_title：Biochemical and biophysical research communications

authors： Morikawa S,Mabuchi Y,Niibe K,Suzuki S,Nagoshi N,Sunabori T,Shimmura S,Nagai Y,Nakagawa T,Okano H,Matsuzaki Y

更新日期：2009-02-20 00:00:00

abstract：:The effect of thyroid hormone and retinoic acid on the expression of CCAAT/enhancer binding proteins (C/EBPs) alpha and beta was investigated in rat liver during development. Congenital hypothyroidism caused a significant decrease in both C/EBP alpha and C/EBP beta gene expression at early stages of postnatal developm...

journal_title：Biochemical and biophysical research communications

authors： Menéndez-Hurtado A,Vega-Núñez E,Santos A,Perez-Castillo A

更新日期：1997-05-29 00:00:00

abstract：:In order to search for novel estrogen-responsive genes, we performed serial analysis of gene expression (SAGE) for estrogen-treated MCF-7 human breast cancer cells. SAGE analysis of 31,000 and 30,856 tags from non-treated and 17 beta-estradiol (E2)-treated cells for 24 h, respectively, facilitated the identification o...

journal_title：Biochemical and biophysical research communications

authors： Inadera H,Hashimoto S,Dong HY,Suzuki T,Nagai S,Yamashita T,Toyoda N,Matsushima K

更新日期：2000-08-18 00:00:00

abstract：:DENN domains are found in a variety of signaling proteins but their exact function remains undefined. Some of the DENN-containing proteins, such as rat Rab3GEP (Rab3 GDP/GTP exchange protein) or mouse Rab6IP1 (Rab6 interacting protein 1) interact with GTPases of the Rab family. Others, such as human MADD (MAP (Mitogen...

journal_title：Biochemical and biophysical research communications

authors： Levivier E,Goud B,Souchet M,Calmels TP,Mornon JP,Callebaut I

更新日期：2001-09-28 00:00:00

abstract：:The extracellular microenvironment plays a key role in regulation of cellular functions and growth control. We show here that membrane-type 1 matrix metalloproteinase (MT1-MMP) acts as a growth promoter in confluent culture. When MT1-MMP was silenced in HT1080 fibrosarcoma cells, cells created three dimensional (3D) f...

journal_title：Biochemical and biophysical research communications

authors： Takino T,Guo L,Domoto T,Sato H

更新日期：2013-07-05 00:00:00

abstract：:In epithelial cells progression through the G1 phase of the cell cycle and preparing the cell for the S phase is regulated by cyclin D1-cdk4. Cells that express the retinoblastoma protein (pRb) are dependent on cyclin D1-cdk4 activity for their proliferation while cells that do not express pRb are not. Overexpression ...

journal_title：Biochemical and biophysical research communications

authors： Kent LL,Hull-Campbell NE,Lau T,Wu JC,Thompson SA,Nori M

更新日期：1999-07-14 00:00:00

abstract：:Polygonatum cyrtonema lectin (PCL), a mannose/sialic acid-binding plant lectin, has recently drawn a rising attention for cancer biologists because PCL bears remarkable anti-tumor activities and thus inducing programmed cell death (PCD) including apoptosis and autophagy in cancer cells. In this review, we focus on exp...

journal_title：Biochemical and biophysical research communications

authors： Wang SY,Yu QJ,Bao JK,Liu B

更新日期：2011-03-25 00:00:00

abstract：:The retinoblastoma suppressor (Rb)-associated protein 46 (RbAp46) is a member of the WD-repeat protein family and a component of the histone modifying and remodeling complexes. Previously, we demonstrated that RbAp46 is a potent growth inhibitor that can suppress the transformed phenotype of tumor cells. To explore th...

journal_title：Biochemical and biophysical research communications

authors： Chen GC,Guan LS,Yu JH,Li GC,Choi Kim HR,Wang ZY

更新日期：2001-06-08 00:00:00