当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Inhibition of Na,K-ATPase activates PI3 kinase and inhibits apoptosis in LLC-PK1 cells.

Inhibition of Na,K-ATPase activates PI3 kinase and inhibits apoptosis in LLC-PK1 cells.

Abstract：

:In the present study we used LLC-PK1 cells, a porcine renal proximal tubular cell line, to investigate whether PI3 kinase activation was involved in the anti-apoptotic effect of ouabain, a specific inhibitor of Na,K-ATPase. Apoptosis was induced by actinomycin D (Act D, 5 microM) and assessed by appearance of hypodiploid nuclei and DNA fragmentation. Ouabain attenuated Act D-induced apoptotic response in a dose-dependent manner. Incubation in a low K(+) medium (0.1 mM) which is another way to decrease Na,K-ATPase activity also had anti-apoptotic effect. Both ouabain and low K(+) medium increased the PI3 kinase activity in p85 immunoprecipitates. Ouabain, as well as incubation in the low K(+) medium, also increased the phosphorylation of Akt. Inhibition of PI3 kinase by either wortmannin or LY294002 reversed the cytoprotective effect of ouabain. These data together indicate that inhibition of Na,K-ATPase activates PI3 kinase in LLC-PK1 cells which could then exert the cytoprotective effect.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Zhou X,Jiang G,Zhao A,Bondeva T,Hirszel P,Balla T

doi

10.1006/bbrc.2001.5126

subject

Has Abstract

pub_date

2001-07-06 00:00:00

pages

46-51

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(01)95126-5

journal_volume

285

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Activation of nit-1 nitrate reductase by W-formate dehydrogenase.

    abstract：:Formate dehydrogenase ( FDH ) from Clostridium thermoaceticum is a known tungsten enzyme. FDH was tested for the presence of nitrogenase-type cofactor and nitrate reductase-type cofactor by the Azotobacter vinelandii UW-45 and Neurospora crassa nit-1 reconstitution assays, respectively. Tungsten formate dehydrogenase ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90782-4

    authors： Deaton JC,Solomon EI,Durfor CN,Wetherbee PJ,Burgess BK,Jacobs DB

    更新日期：1984-06-29 00:00:00

  • Molecular cloning and characterization of a novel human gene containing ankyrin repeat and double BTB/POZ domain.

    abstract：:A novel human gene containing an ankyrin repeat and BTB/POZ domains (BPOZ) was isolated from a human leukocyte cDNA library. The cDNA sequence contains an open reading frame of 1434 bp that encodes 478 amino acid residues with a predicted molecular mass of 53.9 kDa. Sequence pattern analysis shows that BPOZ contains a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3053

    authors： Dai KS,Wei W,Liew CC

    更新日期：2000-07-14 00:00:00

  • Transcription factor levels in medullary thyroid carcinoma cells differentiated by Harvey ras oncogene: c-jun is increased.

    abstract：:In the TT cell line of human medullary thyroid carcinoma, the viral Harvey ras (v-rasH) oncogene induces differentiation, marked by morphological changes, diminution of growth, and increased expression of the calcitonin gene. Here, we show that the transcriptional factor c-jun is increased during v-rasH induced differ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91251-m

    authors： Nelkin BD,Borges M,Mabry M,Baylin SB

    更新日期：1990-07-16 00:00:00

  • Vaspin protects vascular endothelial cells against free fatty acid-induced apoptosis through a phosphatidylinositol 3-kinase/Akt pathway.

    abstract：:Vaspin, an adipocytokine recently identified in a rat model of type 2 diabetes, has been suggested to have an insulin-sensitizing effect. However, the exact mechanism underlying this action has not been fully elucidated. Furthermore, the specific function of vaspin is largely unknown, especially in vascular cells. We ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.08.083

    authors： Jung CH,Lee WJ,Hwang JY,Seol SM,Kim YM,Lee YL,Park JY

    更新日期：2011-09-23 00:00:00

  • A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotective activity.

    abstract：:We previously showed that cor15, a cold-regulated gene from Arabidopsis thaliana, encodes a 15 kDa polypeptide designated COR15. Here we report that COR15 has potent cryoprotective activity in an in vitro cryoprotection assay. Specifically, COR15 was very effective in protecting the cold-labile enzyme lactate dehydrog...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80304-3

    authors： Lin C,Thomashow MF

    更新日期：1992-03-31 00:00:00

  • Restoration of ENaC expression by glucocorticoid receptor transfection in human HT-29/B6 colon cells.

    abstract：:The epithelial sodium channel (ENaC) controls colonic sodium absorption. So far, investigation of ENaC was limited by an unexplained lack of steroid-dependent ENaC expression in cultured intestinal cells, which we aimed to resolve. HT-29/B6 cells constitutively expressed the alpha-ENaC subunit, while beta- and gamma-E...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.04.012

    authors： Zeissig S,Fromm A,Mankertz J,Zeitz M,Fromm M,Schulzke JD

    更新日期：2006-06-16 00:00:00

  • PACS-1 and adaptor protein-1 mediate ACTH trafficking to the regulated secretory pathway.

    abstract：:The regulated secretory pathway is a specialized form of protein secretion found in endocrine and neuroendocrine cell types. Pro-opiomelanocortin (POMC) is a pro-hormone that utilizes this pathway to be trafficked to dense core secretory granules (DCSGs). Within this organelle, POMC is processed to multiple bioactive ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.085

    authors： Dirk BS,End C,Pawlak EN,Van Nynatten LR,Jacob RA,Heit B,Dikeakos JD

    更新日期：2018-12-09 00:00:00

  • Increased gene expression and production of murine endothelin receptors after birth.

    abstract：:We developed the real-time PCR quantification of endothelin-A (ET-A) and endothelin-B (ET-B) receptor genes and present their relative expression levels in various adult tissues and during development in mouse using the 2(-Delta Delta C(T)) method. ET-A and ET-B receptors were detected in all tissues examined. Gene ex...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00769-1

    authors： Adur J,Takizawa S,Quan J,Uchide T,Saida K

    更新日期：2003-06-06 00:00:00

  • A putative tetrapeptide antagonist prevents beta-amyloid-induced long-term elevation of [Ca2+]i in rat astrocytes.

    abstract：:Comparative fluorimetric studies on the long-term (8-hour) action of beta[1-42]amyloid and its shorter fragments beta[1-40], beta[25-35] and beta[31-35] on the steady-state intracellular Ca2+ concentration in primary cultures of rat astroglial cells using the Ca2+-sensitive fluorescent probe Fura-2 AM revealed higher ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6806

    authors： Laskay G,Zarándi M,Varga J,Jost K,Fónagy A,Torday C,Latzkovits L,Penke B

    更新日期：1997-06-27 00:00:00

  • Homozygous deletions of the MTS1 gene are rare in non-astrocytic brain tumors.

    abstract：:Several studies support a role for the multiple tumor suppressor gene (MTS1) in the malignant progression of different tumor types. In this study we have examined the status of the MTS1 gene in a variety of non-astrocytic tumors of the central nervous system. It was not possible, using multiplex PCR with primers for M...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1828

    authors： Walker DG,Duan W,Kaye AH,Lavin MF

    更新日期：1995-06-15 00:00:00

  • Lipopolysaccharide prevents apoptosis induced by brefeldin A, an endoplasmic reticulum stress agent, in RAW 264.7 cells.

    abstract：:The effect of lipopolysaccharide (LPS) on the cell death induced by endoplasmic reticulum (ER) stress agents in RAW 264.7 cells was studied. LPS prevented the cell death by brefeldin A, but not thapsigargin and tunicamycin. CpG DNA as well as LPS prevented brefeldin A-induced cell death whereas tumor necrosis factor-a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.050

    authors： Islam S,Hassan F,Tumurkhuu G,Ito H,Koide N,Mori I,Yoshida T,Yokochi T

    更新日期：2006-02-10 00:00:00

  • Lineage and stage specific expression of HOX 1 genes in the human hematopoietic system.

    abstract：:Recent observations have demonstrated the expression of several members of the homeobox-containing (HOX) gene complexes within the hematopoietic compartment. We have analyzed the expression pattern of the entire HOX 1 locus in a panel of leukemia-derived human cell lines representing various blood phenotypes. The expr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80307-9

    authors： Vieille-Grosjean I,Roullot V,Courtois G

    更新日期：1992-03-31 00:00:00

  • Cation-dependent prolactin activation of guanylate cyclase.

    abstract：:Prolactin enhanced guanylate cyclase [E.C.4.6.1.2] two- to threefold in ovary, testis, mammary gland, liver and kidney. Dose response relationships revealed that maximal activation of this enzyme was at a concentration of one nanomolar and that increasing prolactin's concentration to the millimolar range caused no fur...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80244-2

    authors： Vesely DL

    更新日期：1984-09-28 00:00:00

  • Human lens high-molecular-weight alpha-crystallin aggregates.

    abstract：:Alpha-crystallin high-molecular-weight (HMW) aggregates can be formed in vitro by many mechanisms, but the mechanism of in vivo aggregation has not been clearly established. HMW and LMW (low-molecular-weight) alpha-crystallins were isolated from human lenses 50-60 years of age and some spectroscopic measurements were ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3306

    authors： Liang JJ,Akhtar NJ

    更新日期：2000-08-28 00:00:00

  • Reprogramming of cancer stem cells into non-tumorigenic cells using stem cell exosomes for cancer therapy.

    abstract：:Cancer stem cells (CSCs) are a small population of cells with stem cell-like properties found in tumors. CSCs are closely associated with tumor heterogeneity, which influences tumor progress, metastasis, and drug resistance. Here, we propose a concept to enhance efficacy of cancer therapy through CSC reprogramming int...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.072

    authors： Lee KS,Choi JS,Cho YW

    更新日期：2019-05-07 00:00:00

  • A possible strategy to fight COVID-19: Interfering with spike glycoprotein trimerization.

    abstract：:The recent release of COVID-19 spike glycoprotein allows detailed analysis of the structural features that are required for stabilizing the infective form of its quaternary assembly. Trying to disassemble the trimeric structure of COVID-19 spike glycoprotein, we analyzed single protomer surfaces searching for concave ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.04.007

    authors： Bongini P,Trezza A,Bianchini M,Spiga O,Niccolai N

    更新日期：2020-07-12 00:00:00

  • The dipeptide, gamma-glutamylcysteine, is recognized by the anti-glutathione antibody single chain Fv fragment 20C9.

    abstract：:The anti-glutathione antibody scFv 20C9, which we previously isolated from a human synthetic phage antibody scFv library [Hirose, M., Hayano, T., Shirai, H., Nakamura, H., and Kikuchi, M. (1998) Protein Eng. 11, 243-248], was expressed in the E. coli pET system and purified by sequential chromatography on Ni and gluta...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4491

    authors： Horibe T,Furuya R,Iwai A,Yosho C,Tujimoto Y,Kikuchi M

    更新日期：2001-03-01 00:00:00

  • Caenorhabditis elegans development requires mitochondrial function in the nervous system.

    abstract：:The mitochondrial respiratory chain (MRC) supplies the majority of the energy requirements of most eucaryotic cells. A null mutation in the Caenorhabditis elegans nuo-1 gene encoding a subunit of complex I (NADH-ubiquinone oxidoreductase) is lethal, leading to a developmental arrest at the third larval stage. To ident...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.108

    authors： Ndegwa S,Lemire BD

    更新日期：2004-07-09 00:00:00

  • The IRI release from insulin gene-transduced hepatic cells responds to ambient glucose concentration.

    abstract：:Insulin gene was induced into differentiated hepatic cell line. These cells released immunoreactive insulin (IRI) of which molecular weight was greater than mature insulin. The secretion of IRI was responsive to glucose concentration and inhibited by glucose metabolism antagonist, 2-deoxy glucose (2-DOG). The mRNA of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6445

    authors： Hayashi M,Taguchi M,Fujita T

    更新日期：1997-04-17 00:00:00

  • Ultrastructure of nuclear aggregates formed by expressing an expanded polyglutamine.

    abstract：:Intranuclear inclusions have been observed in the brains of patients affected with Huntington's disease (HD). Neuro 2A cells that transiently expressed HD exon 1 bearing 74 glutamine repeats linked to the green fluorescent protein (GFP) and the nuclear localization sequence (NLS) contained aggregates in nuclei. The ag...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00498-9

    authors： Hazeki N,Tsukamoto T,Yazawa I,Koyama M,Hattori S,Someki I,Iwatsubo T,Nakamura K,Goto J,Kanazawa I

    更新日期：2002-06-07 00:00:00

  • Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells.

    abstract：:The CD133 antigen, identified as a hematopoietic stem cell marker, appears in various human embryonic epithelia including the neural tube, gut, and kidney. We herein investigated whether CD133(+) cells isolated from human hepatocellular carcinoma cell lines possess cancer stem/progenitor cell-like properties. Among th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.128

    authors： Suetsugu A,Nagaki M,Aoki H,Motohashi T,Kunisada T,Moriwaki H

    更新日期：2006-12-29 00:00:00

  • Adipose tissue-deprived stem cells acquire cementoblast features treated with dental follicle cell conditioned medium containing dentin non-collagenous proteins in vitro.

    abstract：:Adipose tissue-derived stem cells (ADSCs), which are easily harvested and show excellent pluripotency potential, have generated considerable interest in regenerative medicine. In this study, the differentiation of ADSCs was assessed after treatment with dental follicle cell conditioned medium (DFCCM) containing dentin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.05.067

    authors： Wen X,Nie X,Zhang L,Liu L,Deng M

    更新日期：2011-06-10 00:00:00

  • The protective effect of tetrahydrobiopterin on the nitric oxide-mediated inhibition of purified nitric oxide synthase.

    abstract：:The nitric oxide synthases (NOS) are a class of enzymes responsible for the generation of NO via an oxygen and NADPH dependent oxidation of the amino acid arginine. These enzymes are ironheme proteins which contain FAD and FMN and, enigmatically, require tetrahydrobiopterin (BH4). NOS has recently been shown to be sub...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1052

    authors： Hyun J,Komori Y,Chaudhuri G,Ignarro LJ,Fukuto JM

    更新日期：1995-01-05 00:00:00

  • Activation of Bak in ultrasound-induced, JNK- and p38-independent apoptosis and its inhibition by Bcl-2.

    abstract：:The molecular mechanisms underlying ultrasound-induced apoptosis remain poorly understood. We have demonstrated that in Jurkat cells, the over-expression of the anti-apoptotic protein Bcl-2 inhibited ultrasound-induced apoptosis, but not necrosis. Inhibition of caspase activity also protected the cells from apoptosis,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.12.055

    authors： Kinoshita M,Eguchi Y,Hynynen K

    更新日期：2007-02-09 00:00:00

  • Efficient generation of transgenic mice by direct intraovarian injection of plasmid DNA.

    abstract：:We established a rapid procedure for obtaining transgenic mice by directly injecting an enhanced green fluorescent protein (EGFP)-expressing plasmid (pIRES-EGFP) into the ovaries of fertile mice. The frequency of transgenic mouse production was determined by pair-mating, and by polymerase chain reaction (PCR) and sequ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.112

    authors： Yang SY,Wang JG,Cui HX,Sun SG,Li Q,Gu L,Hong Y,Liu PP,Liu WQ

    更新日期：2007-06-22 00:00:00

  • Autophagy-mediated cytoplasmic accumulation of p53 leads to apoptosis through DRAM-BAX in cadmium-exposed human proximal tubular cells.

    abstract：:The tumor suppressor p53 is involved in cadmium (Cd)-induced apoptosis and autophagy. However, the regulatory mechanisms of p53 in Cd-induced kidney injury are not well established. Here, we report the role of autophagy in Cd-induced p53 induction in human proximal tubular cells (HK-2). HK-2 cells treated with Cd indu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.12.019

    authors： Lee HY,Oh SH

    更新日期：2021-01-01 00:00:00

  • The characteristic gene expressions of MAPK phosphatases 1 and 2 in hepatocarcinogenesis, rat ascites hepatoma cells, and regenerating rat liver.

    abstract：:mRNA levels of mitogen-activated protein kinase phosphatases, MKP-1 and MKP-2, were determined during chemical hepatocarcinogenesis and during regeneration of rat liver. In chemical hepatocarcinogenesis, the mRNA levels of MKP-1 were increased in primary hepatomas but decreased in rat ascites hepatomas as compared wit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7547

    authors： Yokoyama A,Karasaki H,Urushibara N,Nomoto K,Imai Y,Nakamura K,Mizuno Y,Ogawa K,Kikuchi K

    更新日期：1997-10-29 00:00:00

  • Mermaid, a family of short interspersed repetitive elements, is useful for zebrafish genome mapping.

    abstract：:A family of short interspersed repetitive elements (SINEs), designated mermaid, is present in the genomes of fish, amphibian and primates, but absent in the mouse genome. We have demonstrated that the sequences of the mermaid family are highly polymorphic in the zebrafish genome as in the human genome. We have also sh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0386

    authors： Shimoda N,Chevrette M,Ekker M,Kikuchi Y,Hotta Y,Okamoto H

    更新日期：1996-03-07 00:00:00

  • An alpha-subunit loop structure is required for GM2 activator protein binding by beta-hexosaminidase A.

    abstract：:The alpha- and/or beta-subunits of human beta-hexosaminidase A (alphabeta) and B (betabeta) are approximately 60% identical. In vivo only beta-hexosaminidase A can utilize GM2 ganglioside as a substrate, but requires the GM2 activator protein to bind GM2 ganglioside and then interact with the enzyme, placing the termi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.159

    authors： Zarghooni M,Bukovac S,Tropak M,Callahan J,Mahuran D

    更新日期：2004-11-19 00:00:00

  • PPARγ activates ABCA1 gene transcription but reduces the level of ABCA1 protein in HepG2 cells.

    abstract：:Synthesis of ABCA1 protein in liver is necessary for high-density lipoproteins (HDL) formation in mammals. Nuclear receptor PPARγ is known as activator of ABCA1 expression, but details of PPARγ-mediated regulation of ABCA1 at both transcriptional and post-transcriptional levels in hepatocytes have not still been well ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.053

    authors： Mogilenko DA,Shavva VS,Dizhe EB,Orlov SV,Perevozchikov AP

    更新日期：2010-11-19 00:00:00