Abstract:
:Mesenchymal stem cells (MSCs) are a heterogeneous subset of stromal stem cells isolated from many adult tissues. Previous studies reported that MSCs can differentiate to both mesodermal and neural lineages by a phenomenon referred to as ''dedifferentiation'' or ''transdifferentiation''. However, since MSCs have only been defined in vitro, much of their development in vivo is still unknown. Here, we prospectively identified MSCs in the bone marrow from adult transgenic mice encoding neural crest-specific P0-Cre/Floxed-EGFP and Wnt1-Cre/Floxed-EGFP. EGFP-positive MSCs formed spheres that expressed neural crest stem cell genes and differentiated into neurons, glial cells, and myofibroblasts. Interestingly, we observed MSCs both in the GFP(+) and GFP(-) fraction and found that there were no significant differences in the in vitro characteristics between these two populations. Our results suggest that MSCs in adult bone marrow have at least two developmental origins, one of which is the neural crest.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Morikawa S,Mabuchi Y,Niibe K,Suzuki S,Nagoshi N,Sunabori T,Shimmura S,Nagai Y,Nakagawa T,Okano H,Matsuzaki Ydoi
10.1016/j.bbrc.2009.01.031subject
Has Abstractpub_date
2009-02-20 00:00:00pages
1114-9issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(09)00058-8journal_volume
379pub_type
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