当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Characterization of a mutant GLUT4 lacking the N-glycosylation site: studies in transfected rat adipose cells.

Characterization of a mutant GLUT4 lacking the N-glycosylation site: studies in transfected rat adipose cells.

Abstract：

:GLUT4, the insulin-responsive glucose transporter expressed primarily in muscle and adipose tissue, contains a single N-glycosylation site. We characterized a mutant GLUT4 lacking the N-glycosylation site (Asn57-->Gln) in primary cultures of rat adipose cells. We transiently transfected cells with expression vectors for epitope-tagged GLUT4 containing either wild-type (GLUT4-HA) or mutant (GLN57-HA) cDNA sequences. Expression of GLN57-HA in adipose cells was approximately 10-fold lower than for GLUT4-HA even though mRNA levels for both recombinant transporters were comparable. Biosynthetic labeling studies showed markedly decreased incorporation of [35S]-methionine/cysteine into GLN57-HA relative to GLUT4-HA consistent with either a decreased synthetic rate or accelerated degradation of GLN57-HA. Interestingly, transient transfection of GLUT4-HA and GLN57-HA in COS-7 cells (which do not express endogenous GLUT4) resulted in comparable levels of protein expression for both transporters. Thus, in the physiologically relevant adipose cell, glycosylation of GLUT4 appears to play an important functional role.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ing BL,Chen H,Robinson KA,Buse MG,Quon MJ

doi

10.1006/bbrc.1996.0015

subject

Has Abstract

pub_date

1996-01-05 00:00:00

pages

76-82

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)90015-7

journal_volume

218

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • The impact of CREB and its phosphorylation at Ser142 on inflammatory nociception.

    abstract：:Peripheral noxious stimulation leads to phosphorylation and thereby activation of the transcription factor CREB in the spinal cord. CREB phosphorylation occurs mainly at serine 133, but the phosphorylation site at serine 142 may also be important. We investigated the impact of spinal CREB protein levels and phosphoryl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.07.148

    authors： Niederberger E,Ehnert C,Gao W,Coste O,Schmidtko A,Popp L,Gall CV,Korf HW,Tegeder I,Geisslinger G

    更新日期：2007-10-12 00:00:00

  • DNA induces apoptosis in electroporated human promonocytic cell line U937.

    abstract：:Experimental gene transfer has permitted a wide variety of studies on gene regulation and function. However, possible effects of the introduced DNA on cellular metabolism are not well understood. Here we demonstrated that introduction of DNA into a promonocytic cell line, U937, by electroporation caused extensive cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2388

    authors： Shimokawa T,Okumura K,Ra C

    更新日期：2000-04-02 00:00:00

  • Role of Flightless-I (Drosophila) homolog in the transcription activation of type I collagen gene mediated by transforming growth factor beta.

    abstract：:Flightless-I (Drosophila) homolog (FLII) is a nuclear receptor coactivator that is known to interact with other transcriptional regulators such as the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, at the promoter or enhancer region of estrogen receptor (ER)-α target genes. However, little is known ab...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.10.100

    authors： Lim MS,Jeong KW

    更新日期：2014-11-21 00:00:00

  • Structural characterization of the human estrogen synthetase (aromatase) gene.

    abstract：:The estrogen synthetase (aromatase, cytochrome P-450AROM) gene has been isolated from human genomic libraries and characterized. The restriction map of 43 positive clones obtained indicated that this enzyme is present as a single copy gene. The aromatase gene is unexpectedly large compared with other forms of the cyto...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91954-q

    authors： Harada N,Yamada K,Saito K,Kibe N,Dohmae S,Takagi Y

    更新日期：1990-01-15 00:00:00

  • Granzyme B/perforin-mediated apoptosis of Jurkat cells results in cleavage of poly(ADP-ribose) polymerase to the 89-kDa apoptotic fragment and less abundant 64-kDa fragment.

    abstract：:Cytotoxic lymphocytes utilize granule associated serine proteases (granzymes) and perforin to induce apoptosis. Although the importance of granzyme B has been established by gene ablation experiments, biochemical events initiated by the granzyme remain enigmatic. We show here that exposure of Jurkat cells to granzyme ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1565

    authors： Froelich CJ,Hanna WL,Poirier GG,Duriez PJ,D'Amours D,Salvesen GS,Alnemri ES,Earnshaw WC,Shah GM

    更新日期：1996-10-23 00:00:00

  • Lens-specific expression of a chicken beta A3/A1-crystallin promoter fragment in transgenic mice.

    abstract：:Beta A3/A1-crystallin is one of the major refractive proteins of the chicken eye lens. Previously we showed that a fragment from -382 to +22 bp of the beta A3/A1-crystallin gene functions as a promoter in transfected lens cells. Here we show by use of the bacterial chloramphenicol acetyltransferase reporter gene that ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0635

    authors： McDermott JB,Cvekl A,Piatigorsky J

    更新日期：1996-04-25 00:00:00

  • Murine bone morphogenetic protein-4 gene: existence of multiple promoters and exons for the 5'-untranslated region.

    abstract：:A murine genomic clone of about 16 kilobases encompassing putatively entire bone morphogenetic protein (BMP)-4 gene has been isolated. Comparison with the cDNA clones revealed the existence of at least five exons on the 3' side of the clone. There are two alternative transcriptional start sites, one is for the first e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1523

    authors： Kurihara T,Kitamura K,Takaoka K,Nakazato H

    更新日期：1993-05-14 00:00:00

  • Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1.

    abstract：:This paper reports the identification of a Rho family nucleotide exchange factor termed mNET1 as a candidate-interacting partner for the first PDZ domain of MAGI-1, a membrane-associated guanylate kinase with inverted arrangement of protein-protein interacting modules. mNET1 was identified in a yeast two-hybrid screen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4880

    authors： Dobrosotskaya IY

    更新日期：2001-05-18 00:00:00

  • Cdc2 activation is not required for thymocyte apoptosis.

    abstract：:Apoptosis is a mode of cell death characterized by chromatin condensation and disassembly of the nuclear lamina, processes that are also characteristic of mitosis. The apparent similarity between the two events, together with observations that apoptosis can occur following G2 arrest, has led to the suggestion that apo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2086

    authors： Norbury C,MacFarlane M,Fearnhead H,Cohen GM

    更新日期：1994-08-15 00:00:00

  • Sugarcane Elongin C is involved in infection by sugarcane mosaic disease pathogens.

    abstract：:Sugarcane (Saccharum sp. hybrid) provides the main source of sugar for humans. Sugarcane mosaic disease (SMD) is a major threat to sugarcane production. Currently, control of SMD is mainly dependent on breeding resistant cultivars through hybridization, which is time-consuming. Understanding the mechanism of viral inf...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.015

    authors： Zhai Y,Deng Y,Cheng G,Peng L,Zheng Y,Yang Y,Xu J

    更新日期：2015-10-23 00:00:00

  • Decreased ET(B) receptor expression in human metastatic melanoma cells.

    abstract：:In this study, we examined the endothelin (ET) receptor subtype involved in mitogenic signaling in human primary and metastatic melanoma cell lines. In a reverse transcriptase-polymerase chain reaction (RT-PCR) study, ET(B) mRNA expression in metastatic melanoma cells was decreased from that of primary melanoma. Only ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0303

    authors： Kikuchi K,Nakagawa H,Kadono T,Etoh T,Byers HR,Mihm MC,Tamaki K

    更新日期：1996-02-27 00:00:00

  • Apoptosis of mink lung epithelial cells by co-treatment of low-dose staurosporine and transforming growth factor-beta1 depends on the enhanced TGF-beta signaling and requires the decreased phosphorylation of PKB/Akt.

    abstract：:We demonstrate how co-treatment of low-dose staurosporine (STS) and TGF-beta1, which alone have little effect on cell death, markedly induces apoptosis in Mv1Lu mink lung epithelial cells, but not in its clonal variant R1B cells lacking functional TGF-beta signaling. This process was associated with mitochondria-depen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.01.078

    authors： Ju EM,Choi KC,Hong SH,Lee CH,Kim BC,Kim SJ,Kim IH,Park SH

    更新日期：2005-03-25 00:00:00

  • Tumor promoter inhibition of cellular binding of somatostatin.

    abstract：:Tumor promoting phorbol esters inhibited the binding of 125I-[Tyr11] somatostatin to isolated acinar cells from guinea-pig pancreas. Maximal inhibition reached 69.7 +/- 5% at 1 microM TPA. Receptor affinity was decreased by 2.5-fold without change in binding capacity. The ability of TPA in inhibiting somatostatin bind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90124-x

    authors： Zeggari M,Susini C,Viguerie N,Esteve JP,Vaysse N,Ribet A

    更新日期：1985-04-30 00:00:00

  • Nucleotide sequence of the complementary DNA for turkey growth hormone.

    abstract：:Near-full length complementary DNA (cDNA) clones encoding turkey growth hormone (GH) have been isolated from a pituitary library. The longer of the two turkey GH cDNA clones that were sequenced is 803 base pairs (bp) in length and contains 41 nucleotides of the 5'-untranslated region (UTR), an open reading frame of 64...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80880-0

    authors： Foster DN,Kim SU,Enyeart JJ,Foster LK

    更新日期：1990-12-31 00:00:00

  • Genome editing in pluripotent stem cells: research and therapeutic applications.

    abstract：:Recent progress in human pluripotent stem cell (hPSC) and genome editing technologies has opened up new avenues for the investigation of human biology in health and disease as well as the development of therapeutic applications. Gene editing approaches with programmable nucleases have been successfully established in ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2016.02.113

    authors： Deleidi M,Yu C

    更新日期：2016-05-06 00:00:00

  • The role of EXT1 gene mutation and its high expression of calcitonin gene-related peptide in the development of multiple exostosis.

    abstract：OBJECTIVE:Screening and identifying the gene mutation of EXT1, EXT2 and EXT3 associated with multiple exostosis (ME) and the expression in tumor tissues. METHODS:Nine patients with multiple exostosis were collected and genomic DNA was extracted. Polymerase chain reaction (PCR) amplification and direct sequencing techn...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.115

    authors： Wu ZY,Wang Y,Wang JW,Chen YZ,Guo Y

    更新日期：2018-11-10 00:00:00

  • Characterization of a cascade of protein interactions initiated at the IL-1 receptor.

    abstract：:Prior studies have identified molecules involved in IL-1 signaling that transmit the extracellular stimulus to downstream kinase molecules causing altered transcriptional activity. Many of these investigations have relied heavily on ligand induced protein-protein interactions detected by immuno-coprecipitation to map ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00385-1

    authors： Boch JA,Yoshida Y,Koyama Y,Wara-Aswapati N,Peng H,Unlu S,Auron PE

    更新日期：2003-04-04 00:00:00

  • Topographical requirements for delta opioid ligands: presence of a carboxyl group in position 4 is not critical for deltorphin high delta receptor affinity and analgesic activity.

    abstract：:To investigate the role of the carboxyl group in deltorphin molecules, we have synthesized three new analogues in which the acidic amino acid residues in position 4 of the deltorphins were replaced by non-acidic but hydrophilic amino acids residues. The three analogues, [Ser4]-, [Gln4]-, and [Cys4]-deltorphin, all are...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81335-x

    authors： Misicka A,Lipkowski AW,Fang L,Knapp RJ,Davis P,Kramer T,Burks TF,Yamamura HI,Carr DB,Hruby VJ

    更新日期：1991-11-14 00:00:00

  • Silencer-mediated repression and non-mediated activation of BDNF and c-fos gene promoters in primary glial or neuronal cells.

    abstract：:Although the neuron-restrictive silencer element (NRSE/Regard) has been shown to function as a negative-acting DNA regulatory element to prevent the expression of neuron-specific genes in non-neuronal cells, little is known about its silencing effect on transcription in primary glial cells nor its effect on transcript...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0974

    authors： Tabuchi A,Nakatani C,Nakaoka R,Naruse Y,Kojima T,Mori N,Tsuda M

    更新日期：1999-08-02 00:00:00

  • Heteronuclear 113Cd-1H NMR study of metal coordination in the human retinoic acid receptor-beta DNA binding domain.

    abstract：:The two zinc fingers of the DNA binding domain of the human retinoic acid receptor-beta were labelled with 113Cd. Two- and three-dimensional heteronuclear nuclear magnetic resonance (NMR) experiments show that the first eight conserved cysteine residues coordinate the two zinc ions tetrahedrally. The ninth conserved c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1442

    authors： Knegtel RM,Boelens R,Ganadu ML,George AV,van der Saag PT,Kaptein R

    更新日期：1993-04-30 00:00:00

  • Neuron-specific antigen HPC-1 from bovine brain reveals strong homology to epimorphin, an essential factor involved in epithelial morphogenesis: identification of a novel protein family.

    abstract：:We have already cloned the cDNA for the HPC-1 antigen, a neuron-specific protein antigen from the rat brain. Here we report the molecular cloning of the bovine HPC-1 antigen homologue, and much strong sequence conservation between rat and bovine. By searching the recent protein data base, it was found that the HPC-1 a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91316-i

    authors： Inoue A,Akagawa K

    更新日期：1992-09-16 00:00:00

  • The Eλ(3-1) enhancer is essential for V(D)J recombination of the murine immunoglobulin lambda light chain locus.

    abstract：:Enhancers are essential for long range chromatin opening and the activation of V(D)J recombination at the antigen receptor loci. The murine immunoglobulin lambda light chain locus is a duplicated locus and, using a bacterial artificial chromosome spanning the 3' half of the locus to generate transgenic mice, we have i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：

    authors： Haque SF,Bevington SL,Boyes J

    更新日期：2013-11-15 00:00:00

  • Anthelmintic drug niclosamide enhances the sensitivity of chronic myeloid leukemia cells to dasatinib through inhibiting Erk/Mnk1/eIF4E pathway.

    abstract：:Chronic myeloid leukemia (CML) responds well to BCR-ABL tyrosine kinase inhibitors (TKI), but becomes resistant to TKIs after it progresses to blast phase (BP). Here we show that niclosamide, a FDA-approved anthelmintic drug, enhances the sensitivity of BP-CML cells to dasatinib (2nd generation of BCR-ABL TKI) through...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.08.047

    authors： Liu Z,Li Y,Lv C,Wang L,Song H

    更新日期：2016-09-16 00:00:00

  • Characteristics of hepatitis B surface antigen produced in yeast.

    abstract：:We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91784-x

    authors： Choo KB,Wu SM,Lee HH,Lo SC

    更新日期：1985-08-30 00:00:00

  • Isolation and expression of a full-length cDNA encoding the human GM2 activator protein.

    abstract：:We report the construction of a cDNA clone encoding a functional GM2-activator protein. The sequence of the complete 5' end of the coding region was determined by direct nucleotide sequencing of a fragment generated by multiple RACE PCR procedures from Hela cell cDNA. Specific oligonucleotides were synthesized from th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90671-s

    authors： Xie B,McInnes B,Neote K,Lamhonwah AM,Mahuran D

    更新日期：1991-06-28 00:00:00

  • Effect of boric acid solution on cartilage metabolism.

    abstract：:Pelvic cartilage of chick embryo was used to demonstrate that presence of boron in culture medium decreases synthesis of proteoglycans, collagen and total proteins but on the other hand increases the release of these macromolecules. However, when glucose concentration in culture medium is brought to 22mM, the synthesi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6621

    authors： Benderdour M,Hess K,Gadet MD,Dousset B,Nabet P,Belleville F

    更新日期：1997-05-08 00:00:00

  • Nickel inhibits β-1 adrenoceptor mediated activation of cardiac CFTR chloride channels.

    abstract：:Cardiac ventricular myocytes exhibit a protein kinase A-dependent Cl(-) current (ICl.PKA) mediated by the cystic fibrosis transmembrane conductance regulator (CFTR). There is conflicting evidence regarding the ability of the divalent cation nickel (Ni(2+)), which has been used widely in vitro in the study of other car...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.01.087

    authors： Barman PP,Cheng H,Hancox JC,James AF

    更新日期：2013-03-01 00:00:00

  • The thiol proteinase inhibitors improve the abnormal rapid down-regulation of protein kinase C and the impaired natural killer cell activity in (Chediak-Higashi syndrome) beige mouse.

    abstract：:Protein kinase C (PKC) is essential in intracellular signal transduction for various cell functions including natural killer (NK) cell activity. This enzyme is hydrolysed by calpain, which is Ca2+-dependent thiol proteinase. We showed here that in NK activity-deficient beige (bg/bg) mouse, the model of Chediak-Higashi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92451-0

    authors： Ito M,Sato A,Tanabe F,Ishida E,Takami Y,Shigeta S

    更新日期：1989-04-28 00:00:00

  • Isolation and characterization of four basic proteins from horse eosinophilic granules.

    abstract：:Four new basic proteins were isolated from horse eosinophils and purified. The eosinophils release these proteins after permeabilization with saponin and degranulation stimulized by guanosine 5'-O-thiotriphosphate. The proteins were separated and purified on a Superose P12- and a Mono S-column by fast protein liquid c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1425

    authors： Piller K,Portmann P

    更新日期：1993-04-30 00:00:00

  • Nucleoside triphosphate binding and hydrolysis by histone H1.

    abstract：:We present here further evidence supporting that histone H1 contains a nucleotide binding site interacting e.g. with ADP, ATP, GDP and GTP. The finding is in accordance with the previous observation that nucleotides modulate recognition of DNA by H1. Most interestingly, H1 appears to be capable of hydrolyzing NTPs and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80146-9

    authors： Mannermaa RM,Oikarinen J

    更新日期：1992-01-15 00:00:00