Exosome from chaperone-rich cell lysates-loaded dendritic cells produced by CELLine 1000 culture system exhibits potent immune activity.


:Dendritic cells (DCs) pulsed with exosomes can stimulate efficient cytotoxic T-lymphocyte responses and anti-tumor immunity. However, the quantity of DC-derived exosomes (DCex) obtained from various culture systems is very low, which is a significant practical issue hampering progress in this research area and needs to be addressed. Gliomas were particularly aggressive, with high morbidity and mortality, indicating that this is a form of incurable highly malignant tumor of the brain with poor prognosis. In the present study, we demonstrate that the CELLine 1000 culture system can dramatically increase the production of DCex. The morphology, phenotype and immune molecules of these DCex were found to be identical to those using traditional methods. Our researches supply a cost-effective, useful method for significantly increasing the quantity of exosomes. In addition, GL261 glioma cells were chosen to separate chaperone-rich cell lysates (CRCL). The results indicate that CRCL-GL261 cell lysates can trigger the most intense expression of immune molecules on DCex or DCs, which has important implications for the research into tumor treatment and diagnosis.


Bu N,Wu H,Zhang G,Ma X,Zhao P,Zhai N,Xiang L,Cao H,Yang X,Liu J




Has Abstract


2015-01-02 00:00:00














  • Human monoclonal antibodies produced from L-leucine methyl ester-treated and in vitro immunized peripheral blood lymphocytes.

    abstract::Primary in vitro immunization of peripheral blood lymphocytes has been studied for the production of human monoclonal antibodies. Depletion of a lysosome-rich cell population, containing large granular lymphocytes, monocytes, and a subset of T suppressor cells, was shown to be crucial before the cells could be immuniz...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Borrebaeck CA,Danielsson L,Möller SA

    更新日期:1987-11-13 00:00:00

  • Carbohydrate antigens recognized by anti-horseradish peroxidase antiserum are expressed on mammalian cells.

    abstract::Antiserum raised against horseradish peroxidase (HRP) recognizes neural specific carbohydrate antigens in Drosophila and other insects. The occurrence of the antigens in mammalian cells was investigated. Proteins were extracted from rat pheochromocytoma cells, PC12, and various tissue of rat and bovine origin, and fur...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kurosaka A,Tsuyuki Y,Tahara S

    更新日期:1994-10-14 00:00:00

  • Spore-displayed streptavidin: a live diagnostic tool in biotechnology.

    abstract::Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-stre...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim JH,Lee CS,Kim BG

    更新日期:2005-05-27 00:00:00

  • Catabolism of intact type VI collagen microfibrils: susceptibility to degradation by serine proteinases.

    abstract::We present the first direct biochemical evidence for the turnover of intact type VI collagen microfibrils. Matrix-degrading enzymes of the serine proteinase class, including rat mast cell chymases I and II, human mast cell tryptase, neutrophil elastase, cathepsin G and trypsin, were able to catabolize intact type VI c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kielty CM,Lees M,Shuttleworth CA,Woolley D

    更新日期:1993-03-31 00:00:00

  • Novel cysteine-rich secretory protein in the buccal gland secretion of the parasitic lamprey, Lethenteron japonicum.

    abstract::Lampreys are one of the most primitive vertebrates diverged some 500 million years ago. It has long been known that parasitic lampreys secrete anticoagulant from their buccal glands and prevent blood coagulation of host fishes. We found two major protein components of 160 and 26 kDa in the buccal gland secretion of pa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ito N,Mita M,Takahashi Y,Matsushima A,Watanabe YG,Hirano S,Odani S

    更新日期:2007-06-22 00:00:00

  • Compensatory expression of p73 in PARP-deficient mouse fibroblasts as response to a reduced level of regularly spliced wild-type p53 protein.

    abstract::We have investigated the effect of PARP gene inactivation on the expression of wild-type (wt) p53 protein. Using immortalized fibroblasts from control and PARP knock-out mice we have found by immunoblotting with the PAb421 antibody a profound decrease of the p53 expression to a barely detectable level in PARP knock-ou...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Schmid G,Wang ZQ,Wesierska-Gadek J

    更新日期:1999-02-16 00:00:00

  • Effect of the tyrosine kinase inhibitor lapatinib on CUB-domain containing protein (CDCP1)-mediated breast cancer cell survival and migration.

    abstract::The surface receptor CUB domain-containing protein 1 (CDCP1) is highly expressed in several adenocarcinomas and speculated to participate in anchorage-independent cell survival and cell motility. Tyrosine kinase phosphorylation seems to be crucial for intracellular signaling of CDCP1. Lapatinib, a tyrosine kinase inhi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Seidel J,Kunc K,Possinger K,Jehn C,Lüftner D

    更新日期:2011-10-14 00:00:00

  • Carbon dioxide catalysis of the reaction of peroxynitrite with ethyl acetoacetate: an example of aliphatic nitration by peroxynitrite.

    abstract::The reaction of peroxynitrite anion (O=N-OO-) with CO2 results in the formation of an unstable nitrosoperoxycarbonate anion adduct, O=N-OOCO-2 (1). Adduct 1 can serve as a source for several reactive intermediates, including the nitrocarbonate anion (O2N-OCO-2), the carbonate radical ion/nitrogen dioxide radical pair,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Uppu RM,Pryor WA

    更新日期:1996-12-24 00:00:00

  • Lactosylceramide synthase β-1,4-GalT-V: A novel target for the diagnosis and therapy of human colorectal cancer.

    abstract::Little is known about an oncogenic signal transducer β-1,4-galactosyltransferase-V (β-1,4-GalT-V), in human colorectal cancer. Using quantitative RT-PCR, immunohistochemical staining and ELISA assays, we determined that β-1,4-GalT-V gene/protein expression is specifically increased in human colorectal cancer (CRC) tum...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chatterjee SB,Hou J,Bandaru VVR,Pezhouh MK,Syed Rifat Mannan AA,Sharma R

    更新日期:2019-01-08 00:00:00

  • Physiological validation of photochemical reflectance index (PRI) as a photosynthetic parameter using Arabidopsis thaliana mutants.

    abstract::Non-photochemical quenching (NPQ) is the most important photoprotective system in higher plants. NPQ can be divided into several steps according to the timescale of relaxation of chlorophyll fluorescence after reaching a steady state (i.e., the fast phase, qE; middle phase, qZ or qT; and slow phase, qI). The dissipati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kohzuma K,Hikosaka K

    更新日期:2018-03-25 00:00:00

  • Amyloid beta peptide (Abeta42) activates PLC-delta1 promoter through the NF-kappaB binding site.

    abstract::The abnormal deposition of amyloid beta peptide (Abeta) is a hallmark of Alzheimer's disease (AD). Phospholipase C-delta1 (PLC-delta1) is also known to abnormally accumulate in the brains of AD patients, but no report has addressed the relationship between these two events. This study investigated the effect of Abeta4...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim JY,Kim H,Lee SG,Choi BH,Kim YH,Huh PW,Lee KH,Han H,Rha HK

    更新日期:2003-10-24 00:00:00

  • Monoclonal antibody that blocks phosphoinositide-dependent activation of mouse tumor DNA polymerase alpha.

    abstract::A monoclonal antibody (MaB) against mouse sarcoma DNA polymerase alpha was isolated from the culture medium of an IgG-secreting hybridoma. The MaB demonstrated reactivity against two murine DNA polymerase alpha preparations and a calf thymus DNA polymerase alpha. Murine sarcoma polymerase was activated in vitro by pho...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sylvia VL,Norman JO,Curtin GM,Busbee DL

    更新日期:1986-11-26 00:00:00

  • Detection of antibody to hepatitis C E2/NS1 protein in patients with type C hepatitis.

    abstract::Putative E2/NS1 sequence of hepatitis C virus was expressed in E. coli as a fusion protein with maltose binding protein. Approximately 80 kDa protein was obtained containing 38 kDa E2/NS1 protein. The antibody to this protein was detectable in the same serum from which the sequence was amplified. It was also detectabl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yokosuka O,Ito Y,Imazeki F,Ohto M,Omata M

    更新日期:1992-11-30 00:00:00

  • Recruitment of TBP or TFIIB to a promoter proximal position leads to stimulation of RNA polymerase II transcription without activator proteins both in vivo and in vitro.

    abstract::Eukaryotic transcriptional activators may function, at least in part, to facilitate the assembly of the RNA polymerase II (pol II) preinitiation complex at the core promoter region through their interaction with a subset of components of the basal transcription machinery. Previous studies have shown that artificial te...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Huh JR,Park JM,Kim M,Carlson BA,Hatfield DL,Lee BJ

    更新日期:1999-03-05 00:00:00

  • Cloning, expression, and in vitro activity of human endostatin.

    abstract::Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, is a specific inhibitor of endothelial cell proliferation and angiogenesis. In the present study, we have expressed human endostatin in a yeast expression system (10 mg/L). The recombinant protein was expressed in a soluble form and purified to homogeneity. I...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dhanabal M,Volk R,Ramchandran R,Simons M,Sukhatme VP

    更新日期:1999-05-10 00:00:00

  • Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase.

    abstract::A human cell line established from a patient of an acute monocytic leukemia (THP-1) retained an ability to synthesize and secrete plasma apolipoprotein E like protein. The protein was identified with monospecific antibody raised against human plasma apolipoprotein E. The cells also secreted lipoprotein lipase (EC 3.1....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tajima S,Hayashi R,Tsuchiya S,Miyake Y,Yamamoto A

    更新日期:1985-01-16 00:00:00

  • Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts.

    abstract::A monoclonal antibody, highly selective for the 5'-phosphopyridoxyl group, can be used to detect cytosolic pyridoxal-5'-phosphate binding proteins by an immunoblot procedure. This technique, when applied to sodium borohydride-treated cytosolic extracts obtained from normal rat liver at various stages of development as...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kittler JM,Viceps-Madore D,Cidlowski JA,Thanassi JW

    更新日期:1983-04-15 00:00:00

  • Identification of a long form of cystatin from human saliva by rapid microbore HPLC mapping.

    abstract::Microbore HPLC methodology permits rapid and sensitive mapping of human saliva proteins. Saliva is sampled and processed in less than one hour, greatly reducing the likelihood of artifactual protein degradation. As little as 50 microliters of saliva yields proteins in sufficient quantities and purity to obtain amino t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hawke DH,Yuan PM,Wilson KJ,Hunkapiller MW

    更新日期:1987-06-30 00:00:00

  • Effects of high fat diet on GPR109A and GPR81 gene expression.

    abstract::GPR109A (PUMA-G, NIACR1, HCA(2)) and GPR81 (HCA(1)) are G protein-coupled receptors located predominantly on adipocytes that mediate anti-lipolytic effects. These cell surface receptors give the adipocyte the ability to "sense" metabolic changes in the environment and respond through lipolytic regulation and release o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wanders D,Graff EC,Judd RL

    更新日期:2012-08-24 00:00:00

  • Perillaldehyde attenuates cerebral ischemia-reperfusion injury-triggered overexpression of inflammatory cytokines via modulating Akt/JNK pathway in the rat brain cortex.

    abstract::Perillaldehyde (PAH), one of the major oil components in Perilla frutescens, has anti-inflammatory effects. Few studies have examined the neuroprotective effect of PAH on stroke. So the aim of our study is to investigate the effect of PAH on ischemia-reperfusion-induced injury in the rat brain cortex. Middle cerebral ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu L,Li Y,Fu Q,Ma S

    更新日期:2014-11-07 00:00:00

  • LincRNA TINCR facilitates excessive proliferation and inflammation in post-burn skin fibroblasts by directly binding with SND1 protein and inducing SND1-mediated TGF-β1 expression.

    abstract::In this study, we found that lincRNA-TINCR was significantly upregulated in burn-injured skin tissues in vivo and heat-stimulated dermal fibroblasts in vitro, accompanied by an increase in TGF-β1 expression. TINCR overexpression promoted cell proliferation, colony formation, release of pro-inflammatory factors and exp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Qin G,Song Y,Guo Y,Sun Y,Zeng W

    更新日期:2019-02-19 00:00:00

  • p38 MAPK inhibition is critically involved in VEGFR-2-mediated endothelial cell survival.

    abstract::Vascular endothelial growth factor (VEGF) promotes vasculogenesis, arteriogenesis, and angiogenesis by stimulating proliferation, migration, and cell survival of endothelial cells. VEGF mediates its actions through activation of two receptor tyrosine kinases, VEGFR-1 and VEGFR-2. Serum starvation led to apoptosis of h...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yilmaz A,Kliche S,Mayr-Beyrle U,Fellbrich G,Waltenberger J

    更新日期:2003-07-04 00:00:00

  • N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p-nitrophenyl ester: a novel, high selective and optimal chromogenic active site titrant for human and bovine alpha-, beta- and gamma-thrombin.

    abstract::The serine proteinase catalyzed hydrolysis of N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p- nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) was investigated at pH 6.2 and 21.0 degrees C. The results are consistent with the minimum three-step catalytic mechanism. The acylation step is rate limiting for human...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Balliano G,Milla P,Giordano C,Gallina C,Coletta M,Menegatti E,Rizzi M,Bolognesi M,Ascenzi P

    更新日期:1996-08-14 00:00:00

  • Specific interaction of pRB with a rat genomic DNA fragment, REC11.

    abstract::The retinoblastoma gene product (pRB) has been known to contain a sequence-nonspecific DNA binding activity. It is unknown whether pRB can recognize and bind to specific DNA sequences. We have recently identified a rat genomic DNA fragment, termed REC11, which can interact with rat Cdc37-related protein (RCdc37). In t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ozaki T,Sakiyama S

    更新日期:1996-09-04 00:00:00

  • Characterization of Fc gamma receptor IIb expression within abdominal aortic aneurysm.

    abstract:OBJECTIVE:To evaluate protein profiles and Fc gamma receptor IIb (FCGRIIB) expression in abdominal aortic aneurysm (AAA) compared to the expression in normal aortas. METHODS:We performed a protein array to study the protein expression profiles within AAA and normal aortic tissues. FCGRIIB was found to be significantly...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shi Y,Yang CQ,Wang SW,Li W,Li J,Wang SM

    更新日期:2017-04-01 00:00:00

  • Studies on immunoproteasome in human liver. Part I: absence in fetuses, presence in normal subjects, and increased levels in chronic active hepatitis and cirrhosis.

    abstract::Despite the central role of proteasomes in relevant physiological pathways and pathological processes, this topic is unexpectedly largely unexplored in human liver. Here we present data on the presence of proteasome and immunoproteasome in human livers from normal adults, fetuses and patients affected by major hepatic...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Vasuri F,Capizzi E,Bellavista E,Mishto M,Santoro A,Fiorentino M,Capri M,Cescon M,Grazi GL,Grigioni WF,D'Errico-Grigioni A,Franceschi C

    更新日期:2010-06-25 00:00:00

  • The homology of complement factor C8 gamma chain and alpha-1-microglobulin.

    abstract::The sequence of the complement factor C8 gamma chain shares a remarkable degree of similarity with that of alpha-1-microglobulin, a member of the alpha-2u-globulin superfamily. This superfamily comprises a diverse group of distantly related animal proteins possessing characteristic structural features and similar func...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hunt LT,Elzanowski A,Barker WC

    更新日期:1987-11-30 00:00:00

  • Glutathione S-transferase in yeast: induction of mRNA, cDNA cloning and expression in Escherichia coli.

    abstract::Glutathione S-transferase Y-2 mRNA synthesis was induced in yeast Issatchenkia orientalis approximately 37-fold by cultivation with o-dinitrobenzene. A cDNA library complementary to poly (A)+RNA of I. orientalis grown with o-dinitrobenzene was screened by colony hybridization. Twenty positive clones were obtained from...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tamaki H,Kumagai H,Tochikura T

    更新日期:1990-10-30 00:00:00

  • Isolation and purification of a Clq-anti Clq precipitin ring enhancing protein from sera of patients with rheumatoid arthritis.

    abstract::We have isolated and purified to apparent homogeneity a serum protein which appears to be a biological marker for active rheumatoid arthritis. The protein has been found in the sera in all 44 active rheumatoid arthritis patients thus far studied and is absent from, or present in undetected amounts, in sera from normal...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rosano CL,Parhami N,Hurwitz C

    更新日期:1985-05-16 00:00:00

  • Vesicular monoamine transporter in microvesicles from bovine posterior pituitaries is immunologically similar to but distinct from the chromaffin granule counterpart in its sensitivities to 1-methyl-4-phenylpyridinium and histamine.

    abstract::The microvesicles (MVs) in bovine posterior pituitaries contain the reserpine-sensitive vesicular monoamine transporter (Moriyama et al. (1995) J. Biol. Chem. 270, 11424-11429). An antibody against the N-terminal region of the monoamine transporter from bovine chromaffin granules recognized a polypeptide in the MVs wi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Moriyama Y,Yamada H

    更新日期:1996-04-25 00:00:00