Abstract:
:In order to understand gene regulation during wood formation, we cloned a MYB46-like gene in hybrid aspen, Populus tremula x tremuloides, called Pt x tMYB021. Phylogenetic and paired identity analysis of MYB46-like genes in Populus and Arabidopsis reveals relationships between paralogous pairs of Populus MYB46-like proteins and their Arabidopsis counterparts MYB46 and MYB83, and suggest that Pt x tMYB021 is the ortholog of MYB46. Pt x tMYB021 is expressed mainly in xylem tissues, and transiently expressed Pt x tMYB46 transactivates gene promoters of xylan-active CAZymes GT43A, GT43B and Xyn10A. Analysis of conserved motifs within these promoters identify the sequence CCACCAAC, called ACTYP, which is similar to the AC elements mediating transactivation by MYB transcription factors during lignin biosynthesis. Further analysis by Motif Finder identifies four 6 bp CA-rich motifs overlapping ACTYP, and we show that these motifs are enriched in xylem-specific promoters. We propose that AC-type regulatory elements mediate xylem-specific MYB46-dependent expression of secondary cell wall carbohydrate-active enzymes (CAZymes), besides activating gene expression of lignin biosynthesis enzymes.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Winzell A,Aspeborg H,Wang Y,Ezcurra Idoi
10.1016/j.bbrc.2010.03.101subject
Has Abstractpub_date
2010-04-09 00:00:00pages
848-53issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(10)00565-6journal_volume
394pub_type
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