Determination of the 5' exon structure of the human cardiac alpha-myosin heavy chain gene.

Abstract:

:We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.

authors

Brand NJ,Dabhade N,Yacoub M,Barton PJ

doi

10.1016/0006-291x(91)91707-j

subject

Has Abstract

pub_date

1991-09-30 00:00:00

pages

1255-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91707-J

journal_volume

179

pub_type

杂志文章
  • A fraction of neurofibromin interacts with PML bodies in the nucleus of the CCF astrocytoma cell line.

    abstract::Neurofibromatosis type 1 is a common genetic disease that causes nervous system tumors, and cognitive deficits. It is due to mutations within the NF1 gene, which encodes the Nf1 protein. Nf1 has been shown to be involved in the regulation of Ras, cAMP and actin cytoskeleton dynamics. In this study, using immunofluores...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.01.079

    authors: Godin F,Villette S,Vallée B,Doudeau M,Morisset-Lopez S,Ardourel M,Hevor T,Pichon C,Bénédetti H

    更新日期:2012-02-24 00:00:00

  • The bacteriophage T7 binary system activates transient transgene expression in zebrafish (Danio rerio) embryos.

    abstract::The bacteriophage T7 binary expression system is widely used in vitro for high level selective expression of cloned genes but its application to in vivo models has not yet been investigated. In the present work, we show that coinjection into fertilized zebrafish eggs of pE1T7R, an expression plasmid bearing the T7 RNA...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.7170

    authors: Verri T,Argenton F,Tomanin R,Scarpa M,Storelli C,Costa R,Colombo L,Bortolussi M

    更新日期:1997-08-28 00:00:00

  • Mechanism of the action of the inhibitor of reverse transcriptase.

    abstract::An inhibitor of reverse transcriptase of Moloney leukemia virus was reported previously in the cytoplasm of the cultured cells (1). In this report, the mechanism of the inhibition was examined. The inhibitory activity was completely abrogated by treatment at 55 degrees C for 20 min. Destruction of the reaction product...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(84)90412-1

    authors: Rokutanda M,Watanabe ST,Maeda YY

    更新日期:1984-08-30 00:00:00

  • Decreased expression of protein tyrosine kinase 6 contributes to tumor progression and metastasis in laryngeal squamous cell carcinoma.

    abstract:BACKGROUND:PTK6 is involved in cell proliferation, invasion and migration. Patients with lower PTK6 expression predicts poor prognosis of LSCC. However, the mechanism of PTK6 in LSCC progression remains unclear. We investigated the role of PTK6 in the pathogenesis of LSCC. METHODS:Human LSCC tissues and paired adjacen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.07.051

    authors: Wu D,Zhang X,Liu Z,Yan H,Mai J,Zhao Z,Zhong Q,Liu X

    更新日期:2018-09-10 00:00:00

  • Neurotensin-mediated activation of MAPK pathways and AP-1 binding in the human pancreatic cancer cell line, MIA PaCa-2.

    abstract::Neurotensin (NT), a gastrointestinal (GI) hormone, binds its receptor (NTR) to stimulate proliferation of normal and neoplastic GI tissues; the molecular mechanisms remain largely undefined. Mitogen-activated protein kinases (MAPKs) are a family of intracellular kinases that transmit mitogenic signals by translocating...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2000.2335

    authors: Ehlers RA,Zhang Y,Hellmich MR,Evers BM

    更新日期:2000-03-24 00:00:00

  • Autoantibody to vasoactive intestinal peptide in human circulation.

    abstract::In a radioassay for Vasoactive Intestinal Peptide (VIP)-binding, eight out of 33 plasma samples from healthy human subjects exhibited specific binding ranging from 2.6% to 46.7% of total [125 I]VIP. This binding was competitively displaced by unlabeled VIP. The structurally homologous peptides, Peptide Histidine Isole...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)90442-5

    authors: Paul S,Heinz-Erian P,Said SI

    更新日期:1985-07-16 00:00:00

  • Full-length and truncated versions of the hepatitis B virus (HBV) X protein (pX) transactivate the cmyc protooncogene at the transcriptional level.

    abstract::The products of the human hepatitis B virus (HBV) and woodchuck hepatitis B virus X genes (pXs) transactivate homologous and heterologous genes including the HBV-X and core promoters, the human immunodeficiency viruses 1 (HIV-1) and 2 (HIV-2) long terminal repeats and the beta interferon regulatory sequences. We repor...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)90379-l

    authors: Balsano C,Avantaggiati ML,Natoli G,De Marzio E,Will H,Perricaudet M,Levrero M

    更新日期:1991-05-15 00:00:00

  • Selection of a highly aggressive myeloma cell line by an altered bone microenvironment in the C57BL/KaLwRij mouse.

    abstract::In multiple myeloma (MM), bone marrow microenvironment has an important role for the survival and growth of plasma cells. We previously showed that a high bone turnover, induced by ovariectomy, increased MM cells growth in the 5T2MM model. The present study characterized a new plasma cell line (5THL) which was isolate...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.02.131

    authors: Libouban H,Moreau MF,Baslé MF,Bataille R,Chappard D

    更新日期:2004-04-09 00:00:00

  • TRIM31 interacts with p52(Shc) and inhibits Src-induced anchorage-independent growth.

    abstract::Tripartite motif-containing protein (TRIM) family proteins are involved in a broad range of biological processes and, consistently, their alterations result in diverse pathological conditions such as genetic diseases, viral infection and cancer development. In this study, we found that one of the TRIM family proteins,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.08.028

    authors: Watanabe M,Tsukiyama T,Hatakeyama S

    更新日期:2009-10-16 00:00:00

  • Cumene hydroperoxide-supported demethylation reactions catalyzed by cytochrome P450 2B4 lacking the NH2-terminal sequence.

    abstract::Catalytic activities of cytochrome P450 2B4 lacking NH2-terminal amino acids 2-27 (wt Delta2B4) and that of truncated 2B4 containing a Pro to Ser mutation at position 221 were examined in a system supported by cumene hydroperoxide. Demethylation activities of either truncated 2B4 with N-methylaniline, N,N-dimethylanil...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0569

    authors: Zhang Y,Pernecky SJ

    更新日期:1999-04-29 00:00:00

  • Erbin loss promotes cancer cell proliferation through feedback activation of Akt-Skp2-p27 signaling.

    abstract::Erbin localizes at the basolateral membrane to regulate cell junctions and polarity in epithelial cells. Dysregulation of Erbin has been implicated in tumorigenesis, and yet it is still unclear if and how disrupted Erbin regulates the biological behavior of cancer cells. We report here that depletion of Erbin leads to...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.05.071

    authors: Huang H,Song Y,Wu Y,Guo N,Ma Y,Qian L

    更新日期:2015-07-31 00:00:00

  • Pancreatic cancer stimulates pancreatic stellate cell proliferation and TIMP-1 production through the MAP kinase pathway.

    abstract::Pancreatic adenocarcinoma is characterized by an intense desmoplastic reaction that surrounds the tumor. Pancreatic stellate cells (PSCs) are thought to be responsible for production of this extracellular matrix. When activated, PSCs have a myofibroblast phenotype and produce not only components of the extracellular m...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.08.229

    authors: Yoshida S,Yokota T,Ujiki M,Ding XZ,Pelham C,Adrian TE,Talamonti MS,Bell RH Jr,Denham W

    更新日期:2004-10-29 00:00:00

  • Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice.

    abstract::Cellular prion protein (PrP(C)) is expressed not only in neuronal cells but also in non-neuronal cells such as astroglial cells. In the present study, the prion protein (PrP) gene (Prnp)-deficient astroglial cell line GpL1 from hippocampal cells of ZrchI Prnp(-/-) mice were established. Transfection of Prnp suppressed...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.10.087

    authors: Nishimura T,Sakudo A,Xue G,Ikuta K,Yukawa M,Sugiura K,Onodera T

    更新日期:2008-12-26 00:00:00

  • Tetramethylpyrazine inhibits neutrophil activation following permanent cerebral ischemia in rats.

    abstract::Experimental studies have demonstrated the beneficial effects of tetramethylpyrazine (TMP) against ischemic stroke and highlighted its crucial role in anti-inflammatory activity. This study provides evidence of an alternative target for TMP and sheds light on the mechanism of its anti-inflammatory action against ische...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.05.088

    authors: Chang CY,Kao TK,Chen WY,Ou YC,Li JR,Liao SL,Raung SL,Chen CJ

    更新日期:2015-07-31 00:00:00

  • Phototoxicity and photoinactivation of blebbistatin in UV and visible light.

    abstract::Blebbistatin was recently identified as a selective, cell-permeant inhibitor of myosin II. Because blebbistatin is likely to be used extensively with fluorescence imaging in studies of cytoskeletal dynamics, its compatibility with common excitation wavelengths was examined. Illumination of blebbistatin-treated bovine ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.06.045

    authors: Kolega J

    更新日期:2004-07-30 00:00:00

  • Solution structure of intracellular signal-transducing peptide derived from human beta(2)-adrenergic receptor.

    abstract::The structure of intracellular third loop peptide (betaIII-2: RRSSKFCLKEKKALK) was studied by CD and NMR spectroscopy. According to the CD study, this peptide forms a helix in the TFE solution. The three-dimensional molecular structure in TFE was determined by the 2D (1)H NMR spectroscopy. The structure consists of a ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2002.6606

    authors: Okuda A,Matsumoto O,Akaji M,Taga T,Ohkubo T,Kobayashi Y

    更新日期:2002-03-15 00:00:00

  • Inhibition of the formation of the neurotoxin 5-S-cysteinyl-dopamine by polyphenols.

    abstract::The death of nigral neurons in Parkinson's disease is thought to involve the formation of the endogenous neurotoxin, 5-S-cysteinyl-dopamine. In the present study, we show that the polyphenols, (+)-catechin and caffeic acid, which contain a catechol moiety, inhibit tyrosinase-induced formation of 5-S-cysteinyl-dopamine...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.07.153

    authors: Vauzour D,Vafeiadou K,Spencer JP

    更新日期:2007-10-19 00:00:00

  • Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B' methyltransferase family in Coffea arabica.

    abstract::Trigonelline (N-methylnicotinate), a member of the pyridine alkaloids, accumulates in coffee beans along with caffeine. The biosynthetic pathway of trigonelline is not fully elucidated. While it is quite likely that the production of trigonelline from nicotinate is catalyzed by N-methyltransferase, as is caffeine synt...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.09.043

    authors: Mizuno K,Matsuzaki M,Kanazawa S,Tokiwano T,Yoshizawa Y,Kato M

    更新日期:2014-10-03 00:00:00

  • Extracellular signal-regulated kinase-2 phosphorylates RORalpha4 in vitro.

    abstract::The retinoic acid related orphan receptor RORalpha activates transcription of genes that play an important role in cerebellar development, the protection against age-related degenerative processes, the regulation of inflammatory responses, and is one of the pivotal participants that control the circadian rhythmicity i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.05.016

    authors: Lechtken A,Hörnig M,Werz O,Corvey N,Zündorf I,Dingermann T,Brandes R,Steinhilber D

    更新日期:2007-07-06 00:00:00

  • A protein kinase system from platelet rich plasma.

    abstract::Treatment of platelet rich plasma (PRP) at pH 5 results in the precipitation of a protein kinase system. The protein kinase is associated with the platelet fraction and is capable of phosphorylation of several plasma proteins. Analysis of the 32P-labeled phosphoproteins by two dimensional gel electrophoresis showed th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91736-9

    authors: Galabru J,Krust B,Hovanessian AG

    更新日期:1983-06-15 00:00:00

  • Phosphate disruption and metal toxicity in Saccharomyces cerevisiae: effects of RAD23 and the histone chaperone HPC2.

    abstract::In cells, there exists a delicate balance between accumulation of charged metal cations and abundant anionic complexes such as phosphate. When phosphate metabolism is disrupted, cell-wide spread disturbances in metal homeostasis may ensue. The best example is a yeast pho80 mutant that hyperaccumulates phosphate and as...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.01.047

    authors: Rosenfeld L,Culotta VC

    更新日期:2012-02-10 00:00:00

  • The N588K-HERG K+ channel mutation in the 'short QT syndrome': mechanism of gain-in-function determined at 37 degrees C.

    abstract::The idiopathic short QT syndrome (SQTS) is characterised by an abnormally short QT interval on the electrocardiogram and by an increased risk of arrhythmia and sudden death. One variant of the syndrome is linked to missense mutations that lead to a single amino-acid change (N588K; asparagine to lysine) in the S5-Pore ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.06.112

    authors: McPate MJ,Duncan RS,Milnes JT,Witchel HJ,Hancox JC

    更新日期:2005-08-26 00:00:00

  • Electron transfer between liposomal cytochrome c1 and cytochrome c: catalytic implications of electrostatic potentials.

    abstract::Kinetics measurements of the electron transfer between ferricytochrome c and liposomal ferrocytochrome c1 (with and without the hinge protein) were performed. The observed rate constants(kobs) of electron transfer between liposomal ferrocytochrome c1 and ferricytochrome c at different ionic strengths were measured in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)92132-3

    authors: Kim CH,King TE,Balny C

    更新日期:1989-08-30 00:00:00

  • In vitro codon-reading specificities of unmodified tRNA molecules with different anticodons on the sequence background of Escherichia coli tRNASer.

    abstract::The codon-reading properties of wobble-position variants of the unmodified form of Escherichia coli tRNASer1 (the UGA anticodon) were measured in a cell-free translation system. Two variants, with the AGA and CGA anticodons, each exclusively read a single codon, UCU and UCG, respectively. The only case of efficient wo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.0538

    authors: Takai K,Takaku H,Yokoyama S

    更新日期:1999-04-21 00:00:00

  • Structural determinants for the action of grayanotoxin in D1 S4-S5 and D4 S4-S5 intracellular linkers of sodium channel alpha-subunits.

    abstract::We located a novel binding site for grayanotoxin on the cytoplasmic linkers of voltage-dependent cardiac (rH1) or skeletal-muscle (mu 1) Na(+) channel isoforms (segments S4-S5 in domains D1 and D4), using the alanine scanning substitution method. GTX-modification of Na(+) channels, transiently expressed in HEK 293 cel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)00702-7

    authors: Maejima H,Kinoshita E,Yuki T,Yakehiro M,Seyama I,Yamaoka K

    更新日期:2002-07-12 00:00:00

  • Identification of the domains required for the localization of Prp19p to lipid droplets or the nucleus.

    abstract::Prp19p is a protein found in the nucleus, cytosol or lipid droplets depending on the cell type. Prp19p participates in pre-mRNA splicing, in neuronal/astroglial cell fate decisions or in adipocyte lipid droplet biogenesis. In this study, the motifs of Prp19p that are necessary for its localization to lipid droplets or...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.10.091

    authors: Cho SY,Park PJ,Lee JH,Kim JJ,Lee TR

    更新日期:2007-12-28 00:00:00

  • Promyelocytic leukemia cell line, HL-60, produces human hepatocyte growth factor.

    abstract::The human promyelocytic leukemia cell line, HL-60, stimulated with PMA, produced human HGF-like immunoreactivity (HL-60 HGF), which was detected with human HGF-specific ELISA. The purified HL-60 HGF was indistinguishable from human HGF in the plasma of patients with fulminant hepatic failure by studies of subunit cons...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)81421-4

    authors: Nishino T,Kaise N,Sindo Y,Nishino N,Nishida T,Yasuda S,Masui Y

    更新日期:1991-11-27 00:00:00

  • Identification of four genomic loci highly related to casein-kinase-2-alpha cDNA and characterization of a casein kinase-2-alpha pseudogene within the mouse genome.

    abstract::Using the coding region of the human CK-2 alpha cDNA as a probe for screening a genomic mouse library, positive clones representing four different genomic loci were isolated. Partial DNA sequences of these loci encompassing the first 120 nucleotides of the putative coding region are reported. One positive clone was fu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)90806-v

    authors: Boldyreff B,Wehr K,Hecht R,Issinger OG

    更新日期:1992-07-31 00:00:00

  • Reduction in tumor necrosis factor receptor affinity and cytotoxicity by glucocorticoids.

    abstract::Micromolar concentrations of glucocorticoids rendered L-M cells (a murine tumorigenic fibroblast line) less sensitive to the cytotoxic activity of murine TNF. The potency of different steroids paralleled their known anti-inflammatory potency, and pretreatment was more effective than post treatment. Sex steroids and mi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(88)81238-5

    authors: Kull FC Jr

    更新日期:1988-05-31 00:00:00

  • DNA duplex-supported artificial esterase mimicking by cooperative grafting functional groups.

    abstract::The molecular structures of enzyme mimics may be modified to optimize their catalytic properties. In this study, to generate artificial enzyme mimics, Watson-Crick base paired DNA duplexes were designed as scaffolds which were assembled by nucleotides modified with specific functional groups. This process allowed vari...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.03.106

    authors: Xu L,Ji C,Bai Y,He J,Liu K

    更新日期:2013-05-10 00:00:00