Determination of the 5' exon structure of the human cardiac alpha-myosin heavy chain gene.

Abstract:

:We have deduced the exon structure of the 5' untranslated region of the human cardiac alpha-myosin heavy chain gene by cloning a cDNA for this region using the polymerase chain reaction. Comparison of the cDNA and genomic DNA sequences demonstrates that the 5' non-coding region of the alpha-myosin heavy chain gene is interrupted by two introns of 645 and 337 nucleotides. Secondly we have identified the transcriptional start-site by primer extension, corroborating the previous putative assignment for the alpha-myosin heavy chain promoter based on comparisons between the rat and human genes.

authors

Brand NJ,Dabhade N,Yacoub M,Barton PJ

doi

10.1016/0006-291x(91)91707-j

subject

Has Abstract

pub_date

1991-09-30 00:00:00

pages

1255-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91707-J

journal_volume

179

pub_type

杂志文章
  • A putative prokaryote voltage-gated Ca(2+) channel with only one 6TM motif per subunit.

    abstract::Until now, voltage-gated Ca(2+) channel proteins have been found only in eukaryotes. Here we report that a gene recently discovered in the eubacterium Bacillus halodurans codes for a protein closely related to eukaryotic Ca(2+) channels, but that has only one 6-transmembrane-segement (6TM) motif, instead of four, in i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.4408

    authors: Durell SR,Guy HR

    更新日期:2001-03-02 00:00:00

  • Inhibition, tissue distribution and hormonal specificity of a promoter-binding factor for the uteroglobin gene.

    abstract::We had earlier reported a uteroglobin promoter-binding factor in nuclei from progesterone-stimulated rabbit endometrium that was inhibited by a factor in nuclei without progesterone stimulation or from non-target tissues (Rider, V., and Bullock, D.W., 1988. Biochem. Biophys. Res. Comm. 156, 1368-1375). In the course o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.2455

    authors: Daniel PB,Molloy C,Rayner JC,Hill AM,Bullock DW

    更新日期:1994-10-14 00:00:00

  • Unusual adult-onset Leigh syndrome presentation due to the mitochondrial m.9176T>C mutation.

    abstract::Leigh syndrome (LS) is an incurable, nearly always fatal, neurodegenerative, pediatric disorder that results from respiratory chain failure. The most common mitochondrial DNA (mtDNA) mutations that result in LS are m.8993T→C/G and m.9176T→C/G, which were previously found in several patients with early-onset Leigh synd...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.07.076

    authors: Ronchi D,Bordoni A,Cosi A,Rizzuti M,Fassone E,Di Fonzo A,Servida M,Sciacco M,Collotta M,Ronzoni M,Lucchini V,Mattioli M,Moggio M,Bresolin N,Corti S,Comi GP

    更新日期:2011-08-26 00:00:00

  • Angiotensin II-induced smooth muscle cell migration is mediated by LDL receptor-related protein 1 via regulation of matrix metalloproteinase 2 expression.

    abstract::Angiotensin II (Ang II), one of the main vasoactive hormones of the renin-angiotensin system, contributes to the development and progression of atherosclerosis by inducing vascular smooth muscle cells (VSMCs) migration. Although previous studies have shown that Ang II upregulates low density lipoprotein receptor-relat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.10.019

    authors: Jung HO,Uhm JS,Seo SM,Kim JH,Youn HJ,Baek SH,Chung WS,Seung KB

    更新日期:2010-11-26 00:00:00

  • Regulation of the activity of 5-lipoxygenase, a key enzyme in leukotriene biosynthesis.

    abstract::5-Lipoxygenase (5LO) catalyzes two steps in the biosynthesis of leukotrienes (LTs), lipid mediators of inflammation derived from arachidonic acid. LTs function in normal host defense, and have pathophysiological roles in chronic inflammatory diseases as asthma and atherosclerosis. Also, possible effects of 5LO product...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2010.02.173

    authors: Rådmark O,Samuelsson B

    更新日期:2010-05-21 00:00:00

  • Sofalcone, a gastric mucosa protective agent, increases vascular endothelial growth factor via the Nrf2-heme-oxygenase-1 dependent pathway in gastric epithelial cells.

    abstract::Sofalcone, 2'-carboxymethoxy-4,4-bis(3-methyl-2-butenyloxy)chalcone, is an anti-ulcer agent that is classified as a gastric mucosa protective agent. Recent studies indicate heat shock proteins such as HSP32, also known as heme-oxygenase-1(HO-1), play important roles in protecting gastrointestinal tissues from several ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2010.06.124

    authors: Shibuya A,Onda K,Kawahara H,Uchiyama Y,Nakayama H,Omi T,Nagaoka M,Matsui H,Hirano T

    更新日期:2010-07-30 00:00:00

  • Plasma albumin cysteinylation is regulated by cystathionine beta-synthase.

    abstract::High homocysteine (Hcy) levels are a well-known independent risk factor for endothelial damage in atherosclerosis. We examined whether a rat intestinal model of ischemia-reperfusion was associated with high Hcy and with the modification of plasma albumin into cysteinylated species (CysAlb). The three treatment groups ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.10.191

    authors: Bar-Or D,Curtis CG,Sullivan A,Rael LT,Thomas GW,Craun M,Bar-Or R,Maclean KN,Kraus JP

    更新日期:2004-12-24 00:00:00

  • Physical interaction between MPP8 and PRC1 complex and its implication for regulation of spermatogenesis.

    abstract::Epigenetic modifications such as DNA methylation and histone H3 lysine 27 methylation (H3K27me) are repressive marks that silence gene expression. The M phase phosphoprotein (MPP8) associates with proteins involved in both DNA methylation and histone modifications, and therefore, is a potential candidate to mediate cr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.01.122

    authors: Murata K,Sato S,Haruta M,Goshima T,Chiba Y,Takahashi S,Sharif J,Koseki H,Nakanishi M,Shimada M

    更新日期:2015-03-13 00:00:00

  • Polymorphisms of TNFalpha, IL1beta, and IL1RN genes in chronic obstructive pulmonary disease.

    abstract::It is recognized that genetic factors play a role in the susceptibility to COPD. COPD is characterized by airflow limitation. Chronic inflammation causes small airway disease and parenchymal destruction, leading to the airflow limitation. Polymorphisms in pro-inflammatory cytokine genes may confer a risk for the devel...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.02.099

    authors: Hegab AE,Sakamoto T,Saitoh W,Nomura A,Ishii Y,Morishima Y,Iizuka T,Kiwamoto T,Matsuno Y,Massoud HH,Massoud HM,Hassanein KM,Sekizawa K

    更新日期:2005-04-22 00:00:00

  • Tetrahydromethanopterin methyltransferase, a component of the methane synthesizing complex of Methanobacterium thermoautotrophicum.

    abstract::A new enzyme, tetrahydromethanopterin methyltransferase, which catalyzes the transfer of methyl groups from methyl-tetrahydromethanopterin to 2-mercaptoethane-sulfonate, has been found in the methane synthesizing complex of Methanobacterium thermoautotrophicum. The enzyme is oxygen sensitive and has a well defined pH ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(86)90474-2

    authors: Sauer FD

    更新日期:1986-04-29 00:00:00

  • Human erythrocyte ankyrin, a cytoskeleton component, generates the p57 membrane proteinase which cleaves C3, the third component of complement.

    abstract::Human erythrocytes express a p57 membrane serine proteinase which cleaves C3, the third component of complement. Amino acid analysis of the first 20 N-terminal residues of the purified p57 proteinase demonstrated 100% homology with residues 910-929 of erythrocyte ankyrin, a sequence localized in its Mr = 62 kDa fragme...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.2481

    authors: Hermann J,Barel M,Frade R

    更新日期:1994-10-28 00:00:00

  • Optical spectroscopic observation of a metastable form of sperm whale myoglobin generated by reconstitution.

    abstract::The optical spectrum of Sperm Whale myoglobin, which has been freshly reconstituted with iron protoporphyrin-IX, is shown to be different from that obtained from the native myoglobin, and from the reconstituted, incubated myoglobin (These last two have equivalent absorption spectra). The effect is immediately evident ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)91594-5

    authors: Gebe JA,Peyton DH,Peyton JA

    更新日期:1989-05-30 00:00:00

  • Thermodynamics of i-tetraplex formation in the nuclease hypersensitive element of human c-myc promoter.

    abstract::More than 85% of c-myc transcription is controlled by the nuclease hypersensitive element III(1) upstream of the P1 promoter of this oncogene. The purine-rich sequence in the anti-sense strand forms a G-quadruplex, which has been recently implicated in colorectal cancer, and is proposed as a silencer element [Proc. Na...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.06.074

    authors: Mathur V,Verma A,Maiti S,Chowdhury S

    更新日期:2004-08-06 00:00:00

  • Dexamethasone affects phosphatidylinositol synthesis and degradation in cultured human embryonic cells.

    abstract::Dexamethasone (DEX), a glucocorticoid which induces cleft palate, causes marked alterations in the synthesis and degradation of phosphatidylinositol (PI) but not phosphatidylcholine in an established fibroblastic cell line derived from a human embryonic palate. Incorporation of radiolabeled inositol into phosphatidyli...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91280-9

    authors: Grove RI,Willis WD,Pratt RM

    更新日期:1983-01-14 00:00:00

  • Nucleotide sequence of a bovine lens alpha A-crystallin cDNA.

    abstract::We have determined the nucleotide sequence of a bovine lens alpha A2-crystallin cDNA clone, designated pBL alpha A2-1. The 793 bp cDNA insert contains coding information for the entire 173 amino acid alpha A2-crystallin polypeptide, as well as non-translated sequences located both upstream and downstream from the codi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)91072-2

    authors: Hay RE,Petrash JM

    更新日期:1987-10-14 00:00:00

  • Characterization of p75(+) ectomesenchymal stem cells from rat embryonic facial process tissue.

    abstract::Several populations of stem cells, including those from the dental pulp and periodontal ligament, have been isolated from different parts of the tooth and periodontium. The characteristics of such stem cells have been reported as well. However, as a common progenitor of these cells, ectomesenchymal stem cells (EMSCs),...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.08.109

    authors: Wen X,Liu L,Deng M,Zhang L,Liu R,Xing Y,Zhou X,Nie X

    更新日期:2012-10-12 00:00:00

  • Enhancing the biophysical properties of mRFP1 through incorporation of fluoroproline.

    abstract::Here we enhanced the stability and biophysical properties of mRFP1 through a combination of canonical and non-canonical amino acid mutagenesis. The global replacement of proline residue with (2S, 4R)-4-fluoroproline [(4R)-FP] into mRFP1 led to soluble protein but lost its fluorescence, whereas (2S, 4S)-4-fluoroproline...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2013.09.062

    authors: Deepankumar K,Nadarajan SP,Ayyadurai N,Yun H

    更新日期:2013-11-01 00:00:00

  • Retinoic acid receptors beta and gamma distinguish retinoid signals for growth inhibition and neuritogenesis in human neuroblastoma cells.

    abstract::Retinoids induce marked growth inhibition and neuritic differentiation in human neuroblastoma cells. Expression patterns of nuclear retinoic acid receptors (RAR) in embryonic and adult tissues suggests that RAR subtypes alpha, beta and gamma have tissue-specific functions. We have transfected a human neuroblastoma tum...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.1804

    authors: Cheung B,Hocker JE,Smith SA,Reichert U,Norris MD,Haber M,Stewart BW,Marshall GM

    更新日期:1996-12-04 00:00:00

  • Dihydropyridine antagonists and agonists of calcium channels inhibit the induction of nitric oxide synthase by endotoxin in cultured macrophages.

    abstract::Here we investigate the effects of the dihydropyridine-type antagonists of calcium channels nitrendipine, nimodipine, nisoldipine and the calcium channel agonist BAY K 8644 on the induction of nitric oxide synthase (NOS) by bacterial endotoxin (lipopolysaccharide; LPS) in J774.2 macrophages cultured in vitro. Pretreat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.2323

    authors: Szabó C,Thiemermann C,Vane JR

    更新日期:1993-10-29 00:00:00

  • Phosphatidylinositol 5-phosphate 4-kinase type II beta is required for vitamin D receptor-dependent E-cadherin expression in SW480 cells.

    abstract::Numerous epidemiological data indicate that vitamin D receptor (VDR) signaling induced by its ligand or active metabolite 1α,25-dihydroxyvitamin D(3) (1α,25(OH)(2)D(3)) has anti-cancer activity in several colon cancers. 1α,25(OH)(2)D(3) induces the epithelial differentiation of SW480 colon cancer cells expressing VDR ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.04.045

    authors: Kouchi Z,Fujiwara Y,Yamaguchi H,Nakamura Y,Fukami K

    更新日期:2011-05-20 00:00:00

  • Adenovirus-mediated murine interferon-gamma receptor transfer enhances the efficacy of IFN-gamma in vivo.

    abstract::Interferon gamma (IFN-gamma) plays an important role in immune response, apoptosis, and anti-tumor activity. Its biological activity depends on expression of IFN-gamma receptor (IFN-gammaR). To address whether increased expression of IFN-gammaR is associated in vivo with a higher biological response by IFN-gamma, we c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.6298

    authors: Nishida Y,Maeda Y,Hara A,Arima T,Kimura E,Yamashita S,Uyama E,Mita S,Uchino M

    更新日期:2002-01-25 00:00:00

  • Phosphorylation of the band 4.1-like proteins of the bovine lens.

    abstract::Crude membrane fractions prepared from the superficial cortical fiber cells of bovine lenses were incubated with [gamma-32P]-ATP. Membrane-associated 4.1-like proteins were then immunoprecipitated and analyzed for 32P-incorporation. Band 4.1-like proteins of 150 kd, 80 kd, and 78 kd were found to be labeled in a time-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(85)90308-0

    authors: Aster JC,Brinn K,Maisel H,Brewer GJ

    更新日期:1985-02-15 00:00:00

  • NF-kappaB functions in osteoclasts.

    abstract::NF-kappaB is a pleiotropic transcription factor, which regulates osteoclast formation, function, and survival. The finding that the deletion of both NF-kappaB p50 and p52 subunits resulted in osteopetrosis due to the absence of osteoclasts was followed by the observation that NF-kappaB is essential for RANK-expressing...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2008.10.146

    authors: Soysa NS,Alles N

    更新日期:2009-01-02 00:00:00

  • Metabolism of lipoxins A4 and B4 and of their all-trans isomers by human leukocytes and rat liver microsomes.

    abstract::Incubation of lipoxin A4 (LXA4) either with human leukocytes or with rat liver microsomes in the presence of NADPH very selectively led to a more polar metabolite retaining the conjugated tetraenic structure of LXA4. Lipoxin B4 (LXB4) underwent a very similar metabolism into a more polar metabolite, whereas the all-tr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)91958-f

    authors: Boucher JL,Delaforge M,Mansuy D

    更新日期:1991-05-31 00:00:00

  • RVX-297- a novel BD2 selective inhibitor of BET bromodomains.

    abstract::Bromodomains are epigenetic readers that specifically bind to the acetyl lysine residues of histones and transcription factors. Small molecule BET bromodomain inhibitors can disrupt this interaction which leads to potential modulation of several disease states. Here we describe the binding properties of a novel BET in...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.06.021

    authors: Kharenko OA,Gesner EM,Patel RG,Norek K,White A,Fontano E,Suto RK,Young PR,McLure KG,Hansen HC

    更新日期:2016-08-12 00:00:00

  • C-propeptide region of human pro alpha 1 type 1 collagen interacts with thioredoxin.

    abstract::Thioredoxin (TRX) is one of major components of thiol reducing systems. To investigate the molecular mechanism of TRX function in the lung tissue, we screened a human lung epithelial cell cDNA library for TRX-binding protein by yeast two-hybrid systems. We isolated a plasmid containing C-propeptide region of human pro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(02)00727-1

    authors: Matsumoto K,Masutani H,Nishiyama A,Hashimoto S,Gon Y,Horie T,Yodoi J

    更新日期:2002-07-19 00:00:00

  • A new calcineurin inhibition domain in Cabin1.

    abstract::Calcineurin (CN), a calcium-activated phosphatase, plays a critical role in various biological processes including T cell activation. Cabin1, a calcineurin binding protein 1, has been shown to bind directly to CN using its C-terminal region and inhibit CN activity. However, no increase in CN activity has been found in...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.05.066

    authors: Jang H,Cho EJ,Youn HD

    更新日期:2007-07-20 00:00:00

  • Neural precursor cells differentiated from mouse embryonic stem cells relieve symptomatic motor behavior in a rat model of Parkinson's disease.

    abstract::Pluripotent embryonic stem (ES) cells are the most versatile cells, with the potential to differentiate into all types of cell lineages including neural precursor cells (NPCs), which can be expanded in large numbers for significant periods of time to provide a reliable cell source for transplantation in neurodegenerat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.10.210

    authors: Xu H,Fan X,Wu X,Tang J,Yang H

    更新日期:2005-01-07 00:00:00

  • Changes in tissue concentration of prostaglandins during endochondral bone differentiation.

    abstract::Prostaglandins are known to be involved in bone metabolism as evidenced by the ability of PGE2 to induce bone resorption. It was, therefore, of interest to determine if there was an association of specific prostaglandin metabolites with the various stages of developing bone by utilizing the matrix-induced endochondral...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91660-1

    authors: Wientroub S,Wahl LM,Feuerstein N,Winter CC,Reddi AH

    更新日期:1983-12-28 00:00:00

  • Binding specificity of Lactobacillus to glycolipids.

    abstract::Lactobacillus, a representative useful bacterium, in the intestinal tract was found to bind to some specific glycosphingolipids, like the pathogenic intestinal bacteria. Thin layer chromatography overlay assays using rabbit antiserum against Lactobacillus casei revealed that the bacteria bound to GA1 and trihexosylcer...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.1630

    authors: Yamamoto K,Miwa T,Taniguchi H,Nagano T,Shimamura K,Tanaka T,Kumagai H

    更新日期:1996-11-01 00:00:00