Successful genetic transduction in vivo into synovium by means of electroporation.

abstract：:Ribose methylation of eukaryotic rRNA is directed by box C/D small nucleolar RNAs (snoRNAs), which pinpoint the nucleotide to be methylated in specific position within the rRNA sequence. Here, we report the identification of a novel double-guide C/D box snoRNA termed snR88 that directs methylation of two previously un...

journal_title：Biochemical and biophysical research communications

authors： Bi YZ,Qu LH,Zhou H

更新日期：2007-03-02 00:00:00

abstract：:Retroviruses and retrotransposons depend on their host cells to complete their replication cycles. In many cases, the viral step of reverse transcription is blocked when host cells are arrested in their cell cycle and this block is only released when the host cell resumes division. It has previously been shown that a ...

journal_title：Biochemical and biophysical research communications

authors： Karst SM,Sadeghi N,Menees TM

更新日期：1999-01-27 00:00:00

abstract：:Previous genetic evidence led to the conclusion that proteinase B of yeast was not involved in the function of chitin synthetase 1 (Chs1), based on the demonstration of normal septum formation, cell division and chitin deposition in mutants devoid of the proteinase (Zubenko, G.S., Mitchell, A.P., and Jones, E.W. (1979...

journal_title：Biochemical and biophysical research communications

authors： Silverman SJ,Shaw JA,Cabib E

更新日期：1991-01-15 00:00:00

abstract：:Recent data has suggested a role for nitric oxide (NO) both in the induction of immunity and as an effector of tissue injury in experimental models of inflammation. In this study, we have tested the efficacy of two inhibitors of NO synthase, NG-monomethyl-L-arginine (L-NMMA) and aminoguanidine (AG), to modify the auto...

journal_title：Biochemical and biophysical research communications

authors： Woolfson RG,Qasim FJ,Thiru S,Oliveira DB,Neild GH,Mathieson PW

更新日期：1995-12-14 00:00:00

abstract：:To gain further insight into the biochemical properties of the antibacterial hexetidine, isolated rat liver mitochondria were added with this drug and investigation made of certain features related to mitochondrial bioenergetics. Hexetidine was found to cause oxidation of intramitochondrial pyridine nucleotides and st...

journal_title：Biochemical and biophysical research communications

authors： D'Arcangelo G,Barile M,Passarella S,Quagliariello E

更新日期：1987-09-15 00:00:00

abstract：:The mouse gene encoding the mu opioid receptor, Oprm, undergoes extensive alternatively splicing, with 14 variants having been identified. However, only one variant of human mu opioid receptor gene (Oprm), MOR-1A, has been described. We now report two novel splice variants of the human Oprm gene, hMOR-1O and hMOR-1X. ...

journal_title：Biochemical and biophysical research communications

authors： Pan YX,Xu J,Mahurter L,Xu M,Gilbert AK,Pasternak GW

更新日期：2003-02-21 00:00:00

abstract：:Chalcone and stilbene synthases (CHS and STS) catalyze condensation reactions of p-coumaroyl-CoA and three C(2)-units from malonyl-CoA, but catalyze different cyclization reactions to produce naringenin chalcone and resveratrol, respectively. Condensing activities of wild-type CHS and STS as well as STS-C60S mutant we...

journal_title：Biochemical and biophysical research communications

authors： Suh DY,Kagami J,Fukuma K,Sankawa U

更新日期：2000-09-07 00:00:00

abstract：:To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulations, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-...

journal_title：Biochemical and biophysical research communications

authors： Musso M,Ghiorzo P,Fiorentini P,Giuffrida R,Ciotti P,Garré C,Ravazzolo R,Bianchi-Scarrà G

更新日期：1996-06-05 00:00:00

abstract：:We studied about the physicochemical stability of a novel heparin-binding growth/differentiation factor, midkine (MK). It was found that synthetic human MK was heat and acid stable. Neither incubation at 80 degrees C for 90 sec nor treatment at low pH affected the elution profile of MK molecule on the high performance...

journal_title：Biochemical and biophysical research communications

authors： Kojima S,Inui T,Muramatsu H,Kimura T,Sakakibara S,Muramatsu T

更新日期：1995-11-13 00:00:00

abstract：:Hepatitis delta virus (HDV) is a human pathogen causing fulminant hepatitis and liver cirrhosis. HDV has a circular single-stranded RNA genome, which encodes only a single protein, hepatitis delta antigen (HDAg), from the antigenomic strand. Although the functional roles of HDAg have been extensively studied, the mole...

journal_title：Biochemical and biophysical research communications

authors： Hwang SB,Park KJ,Kim YS

更新日期：1998-03-27 00:00:00

abstract：:The human gene Teratocarcinoma-derived growth factor 1 (TDGF1)/Cripto-1/CR-1 which is expressed in a wide variety of human carcinomas is a member of the EGF-cripto FRL1 cryptic (EGF-CFC) gene family. A majority of human colorectal tumors and hepatomas are known to possess a constitutively active canonical Wnt/beta-cat...

journal_title：Biochemical and biophysical research communications

authors： Hamada S,Watanabe K,Hirota M,Bianco C,Strizzi L,Mancino M,Gonzales M,Salomon DS

更新日期：2007-03-30 00:00:00

abstract：:Near-full length complementary DNA (cDNA) clones encoding turkey growth hormone (GH) have been isolated from a pituitary library. The longer of the two turkey GH cDNA clones that were sequenced is 803 base pairs (bp) in length and contains 41 nucleotides of the 5'-untranslated region (UTR), an open reading frame of 64...

journal_title：Biochemical and biophysical research communications

authors： Foster DN,Kim SU,Enyeart JJ,Foster LK

更新日期：1990-12-31 00:00:00

abstract：:A novel nucleic acid analogue (2Cl-C.OXT-A) significantly stimulated tube formation of human umbilical endothelial cells (HUVEC). Its maximum potency at 100muM was stronger than that of vascular endothelial growth factor (VEGF), a positive control. At this concentration, 2Cl-C.OXT-A moderately stimulated proliferation...

journal_title：Biochemical and biophysical research communications

authors： Tsukamoto I,Sakakibara N,Maruyama T,Igarashi J,Kosaka H,Kubota Y,Tokuda M,Ashino H,Hattori K,Tanaka S,Kawata M,Konishi R

更新日期：2010-09-03 00:00:00

abstract：:Expression of the MHC class I chain related molecules A and B (MICA/B) on tumor cell surface can signal the immune receptor NKG2D for tumor immune destruction. However, MIC was found to be shed by tumors in cancer patients, which negatively regulates host immunity and promotes tumor immune evasion and progression. The...

journal_title：Biochemical and biophysical research communications

authors： Wang X,Lundgren AD,Singh P,Goodlett DR,Plymate SR,Wu JD

更新日期：2009-09-25 00:00:00

abstract：:A PCR-based Sma I restriction fragment length polymorphism (RFLP) in the human D4 dopamine receptor (DRD4) gene was investigated in 200 subjects each of Japanese and Mongolian populations. When compared to allele frequencies for Caucasians previously reported by Petronis et al., Japanese and Mongolian populations show...

journal_title：Biochemical and biophysical research communications

authors： Nakatome M,Honda K,Tun Z,Wakasugi C

更新日期：1996-03-27 00:00:00

abstract：:Human lung carcinoma cells (A549) were oxidatively stressed with mildly-toxic or non-toxic amounts of hydrogen peroxide (H2O2, 0.1 mM to 120 mM) for 5 min. Hydrogen peroxide exposure resulted in a dose dependent inhibition of binding (pH 7) of the thiol reagent iodoacetic acid (IAA) to a 38 kDa cell protein. Incubatio...

journal_title：Biochemical and biophysical research communications

authors： Brodie AE,Reed DJ

更新日期：1987-10-14 00:00:00

abstract：:[3H]cyclo(His-Pro) bound with high affinity (59 nM) to a single class of sites in rat liver plasma membranes, without significant tracer degradation during equilibration for 60 min at 0 degrees C. Binding was specific and saturable (3.9 pmol/mg protein), and were increased by the addition of K+, Mg++ and Na+ at optima...

journal_title：Biochemical and biophysical research communications

authors： Mori M,Yamada M,Yamaguchi M,Suzuki M,Ohshima K,Kobayashi I,Kobayashi S

更新日期：1986-01-14 00:00:00

abstract：:Due to their immune stimulating and apoptosis inducing properties, ligands of the TNF family attract increasing interest as therapeutic proteins. A general limitation of in vivo applications of recombinant soluble TNF ligands is their notoriously rapid clearance from circulation. To improve the serum half life of the ...

journal_title：Biochemical and biophysical research communications

authors： Müller N,Schneider B,Pfizenmaier K,Wajant H

更新日期：2010-06-11 00:00:00

abstract：:After a peripheral nerve injury, the remaining Schwann cells undergo proliferation and adopt a migratory phenotype to prepare for the regeneration of nerves. Celsr2 has been reported to play an important role in the development and maintenance of the function of the nervous system. However, the role and mechanism of C...

journal_title：Biochemical and biophysical research communications

authors： Zhou X,Zhan Z,Tang C,Li J,Zheng X,Zhu S,Qi J

更新日期：2020-12-17 00:00:00

abstract：:Genomic imprinting is an epigenetic phenomenon by which a subset of genes is asymmetrically expressed in a parent-of-origin manner. However, little is known regarding the epigenetic behaviors of imprinted genes during human development. Here, we show dynamic epigenetic changes in imprinted genes in hESCs during in vit...

journal_title：Biochemical and biophysical research communications

authors： Park SW,Kim J,Park JL,Ko JY,Im I,Do HS,Kim H,Tran NT,Lee SH,Kim YS,Cho YS,Lee DR,Han YM

更新日期：2014-04-04 00:00:00

abstract：:As part of our studies on polyamine biosynthesis in yeast, the metabolism of methylthioadenosine was studied in a mutant that lacks methylthioadenosine phosphorylase (meu1delta). The nucleoside accumulates in this mutant and is mainly excreted into the culture medium. Intracellular accumulation of the nucleoside is en...

journal_title：Biochemical and biophysical research communications

authors： Chattopadhyay MK,Tabor CW,Tabor H

更新日期：2006-04-28 00:00:00

abstract：:We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc...

journal_title：Biochemical and biophysical research communications

authors： Shimizu N,Kawamoto JK,Utani K

更新日期：2007-08-03 00:00:00

abstract：:Tyrosine kinase inhibitors targeting the BCR-ABL oncoprotein in chronic myeloid leukemia (CML) are remarkably effective inducing deep molecular remission in most patients. However, they are less effective to eradicate the leukemic stem cells (LSC), resulting in disease persistence. Therefore, there is great need to de...

journal_title：Biochemical and biophysical research communications

authors： Dolinska M,Piccini A,Wong WM,Gelali E,Johansson AS,Klang J,Xiao P,Yektaei-Karin E,Strömberg UO,Mustjoki S,Stenke L,Ekblom M,Qian H

更新日期：2017-08-19 00:00:00

abstract：:Cell membranes are vitally important to living cells. Although the infrastructure of biological membrane is provided by the lipid bilayer, membrane proteins perform most of the specific functions. Knowledge of membrane protein types often provides crucial hints toward determining the function of an uncharacterized mem...

journal_title：Biochemical and biophysical research communications

authors： Liu H,Wang M,Chou KC

更新日期：2005-10-28 00:00:00

abstract：:In higher plants, a cis-acting element, DRE/CRT, is involved in gene expression responsive to drought and low-temperature stress. To understand signal transduction pathways from the cold stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB1A and CBF1 in Arabidopsis thalian...

journal_title：Biochemical and biophysical research communications

authors： Shinwari ZK,Nakashima K,Miura S,Kasuga M,Seki M,Yamaguchi-Shinozaki K,Shinozaki K

更新日期：1998-09-08 00:00:00

abstract：:Within the first external loop of mouse and human TRESK subunits one or two N-glycosylation consensus sites were identified, respectively. Using site directed mutagenesis and Western immunoblotting a single residue of both orthologues was found to be glycosylated upon heterologous expression. Two-electrode voltage-cla...

journal_title：Biochemical and biophysical research communications

authors： Egenberger B,Polleichtner G,Wischmeyer E,Döring F

更新日期：2010-01-08 00:00:00

abstract：:PRPK (p53-related protein kinase) has been reported as a novel protein kinase which binds to the tumor suppressor protein p53 and induces phosphorylation of p53 at Ser 15. To identify novel binding partners of PRPK, we performed a yeast two-hybrid screening and isolated an expressed sequence tag CGI-121 by which a 20-...

journal_title：Biochemical and biophysical research communications

authors： Miyoshi A,Kito K,Aramoto T,Abe Y,Kobayashi N,Ueda N

更新日期：2003-04-04 00:00:00

abstract：:In mammals, pigments are made by melanocytes within a specialized organelle, the melanosome. Mature, pigment-laden melanosomes are then transferred to keratinocytes to drive the visible pigmentation of the animal's hair and skin. The dilute suppressor (dsu) locus encodes an extragenic suppressor of the pigmentation de...

journal_title：Biochemical and biophysical research communications

authors： Wu XS,Martina JA,Hammer JA 3rd

更新日期：2012-09-21 00:00:00

abstract：:As a typical organism of platyhelminth, Dugesia japonica attracts more and more attention for its strong regenerative ability. Protein arginine methyltransferase (PRMT) family is composed of a class of enzymes with methyltransferase activities, which play fundamental roles in vivo in many important physiological proce...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Wu W,Gao L,Chen M,Liu X,Huang M,Li A,Zheng K,Liu D,Deng H,Zhao B,Liu B,Pang Q

更新日期：2020-04-02 00:00:00

abstract：:Mutant superoxide dismutase 1 (mSOD1) is often found as aggregates at the outer-membrane of mitochondria in motor neurons of various mouse models and familial amyotrophic lateral sclerosis (f-ALS) patients. It has been postulated that disruption of mitochondrial function by physical association of misfolded mSOD1 aggr...

journal_title：Biochemical and biophysical research communications

authors： Wei R,Bhattacharya A,Chintalaramulu N,Jernigan AL,Liu Y,Van Remmen H,Chaudhuri AR

更新日期：2012-01-27 00:00:00