当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Identification and characterization of two new human mu opioid receptor splice variants, hMOR-1O and hMOR-1X.

Identification and characterization of two new human mu opioid receptor splice variants, hMOR-1O and hMOR-1X.

Abstract：

:The mouse gene encoding the mu opioid receptor, Oprm, undergoes extensive alternatively splicing, with 14 variants having been identified. However, only one variant of human mu opioid receptor gene (Oprm), MOR-1A, has been described. We now report two novel splice variants of the human Oprm gene, hMOR-1O and hMOR-1X. The full-length cDNAs of hMOR-1O and hMO-1X contained the same exons 1, 2, and 3 as the original hMOR-1, but with exon O or exon X as the alternative fourth exon, respectively. Northern blots revealed several bands with the exon O probe in both human neuroblastoma BE(2)C cells and human brain and a single band (5.5kb) with the exon X probe in selected human brain regions. When transfected into CHO cells, both variants showed high selectivity for mu opioids in binding assays. These two new human mu opioid receptors are the first human MOR-1 variants containing new exons and suggest that the complex splicing present in mice may extend to humans.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Pan YX,Xu J,Mahurter L,Xu M,Gilbert AK,Pasternak GW

doi

10.1016/s0006-291x(03)00089-5

subject

Has Abstract

pub_date

2003-02-21 00:00:00

pages

1057-61

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006291X03000895

journal_volume

301

pub_type

杂志文章

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