当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector.

Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector.

Abstract：

:We report here the production of transgenic quails using a replication-defective pantropic retroviral vector based on Moloney murine leukemia virus (MoMLV) pseudotyped with vesicular stomatitis virus G protein (VSV-G). The retroviral vector was injected into laid quail embryos at the blastodermal stage, and the embryos were incubated to hatch to produce G(0) transgenic quails. Among 134 embryos subjected to viral injection, 37 hatched. The viral vector sequence was detected in the tissues of all G(0) quails. The germ-line transmission efficiency of G(0) quails mated with nontransgenic quails was more than 80% on average. Southern blot analysis revealed that the G(1) transgenic progeny had one to three copies of the transgene. The expression of vector-encoded neomycin-resistance gene under the control of the Rous sarcoma virus (RSV) promoter was observed in several tissues including heart and muscle of both G(1) and G(2) transgenic offspring. Due to the high frequency of germ-line transmission, this method may markedly facilitate the production of transgenic avian.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mizuarai S,Ono K,Yamaguchi K,Nishijima K,Kamihira M,Iijima S

doi

10.1006/bbrc.2001.5422

subject

Has Abstract

pub_date

2001-08-24 00:00:00

pages

456-63

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X01954221

journal_volume

286

pub_type

杂志文章

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