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Cyclo (His-Pro), a metabolite of thyrotropin-releasing hormone: specific binding to rat liver membranes.

Abstract：

:[3H]cyclo(His-Pro) bound with high affinity (59 nM) to a single class of sites in rat liver plasma membranes, without significant tracer degradation during equilibration for 60 min at 0 degrees C. Binding was specific and saturable (3.9 pmol/mg protein), and were increased by the addition of K+, Mg++ and Na+ at optimal concentrations, but not of Ca++ at all concentrations tested. In vivo administration of cyclo(His-Pro), but not thyrotropin-releasing hormone, to rats caused the downregulation of cyclo(His-Pro)-binding sites with decreases in specific binding numbers but did not change binding affinity.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mori M,Yamada M,Yamaguchi M,Suzuki M,Ohshima K,Kobayashi I,Kobayashi S

doi

10.1016/0006-291x(86)90583-8

subject

Has Abstract

pub_date

1986-01-14 00:00:00

pages

443-51

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(86)90583-8

journal_volume

134

pub_type

杂志文章

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