当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Crystal structure of the PSPTO-PSP protein from Pseudomonas syringae pv. tomato str. DC3000 in complex with D-glucose.

Crystal structure of the PSPTO-PSP protein from Pseudomonas syringae pv. tomato str. DC3000 in complex with D-glucose.

Abstract：

:The perchloric acid-soluble protein (PSP) is an endoribonuclease and on the basis of sequence similarity has been assigned to the YjgF/YER057c/UK114 family. These family members are ubiquitous and highly conserved in evolution, and participate in regulating basic cellular metabolism. Here we present the 2.1A crystal structure of the PSP protein from Pseudomonas syringae pv. tomato str. DC3000 (PSPTO-PSP), in complex with D-glucose. The quaternary structure of PSPTO-PSP is a homologous trimer. Glucose is located in the cavity between each two monomers. Comparison of the hydrogen bonds between ligands and YjgF/YER057c/UK114 family homologues confirms that the conserved Arg(103) of PSPTO-PSP is a key amino acid in this cavity for ligand binding. It indicated that the involvement of PSPTO-PSP in essential cellular mechanism was regulated by glucose occupying this active site.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Zhang HM,Gao Y,Li M,Chang WR

doi

10.1016/j.bbrc.2010.05.071

subject

Has Abstract

pub_date

2010-06-18 00:00:00

pages

82-6

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(10)00962-9

journal_volume

397

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Molecular mechanisms of L-selectin-induced co-localization in rafts and shedding [corrected].

    abstract：:Leukocyte recruitment to lymph nodes or inflammatory sites is regulated by adhesion and activation. L-selectin (CD62L) is expressed on leukocytes and mediates tethering and rolling of leukocytes on endothelial cells. Upon stimulation L-selectin is down-regulated by proteolytic cleavage but the molecular mechanisms reg...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02886-3

    authors： Phong MC,Gutwein P,Kadel S,Hexel K,Altevogt P,Linderkamp O,Brenner B

    更新日期：2003-01-10 00:00:00

  • Tributyltin causes cytochrome C release from isolated mitochondria by two discrete mechanisms.

    abstract：:Using isolated liver mitochondria we show that low concentrations of TBT (0.5 microM) cause the release of mitochondrial cytochrome c, in the presence of Ca(2+). This is reflected in a rapid loss of membrane potential (DeltaPsi(m)), and a large-amplitude swelling characteristic of mitochondrial permeability transition...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6679

    authors： Gogvadze V,Stridh H,Orrenius S,Cotgreave I

    更新日期：2002-04-12 00:00:00

  • Promoter analysis and chromosomal mapping of human EBAG9 gene.

    abstract：:The human EBAG9 was previously identified as an estrogen responsive gene using CpG-genomic binding site cloning (Watanate et al., (1998) Mol. Cell. Biol. 18: 442-449). Recently it was revealed that the EBAG9 is identical with RCAS1 which is a cancer cell surface antigen implicated in immune escape. Here, we isolated a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2920

    authors： Ikeda K,Sato M,Tsutsumi O,Tsuchiya F,Tsuneizumi M,Emi M,Imoto I,Inazawa J,Muramatsu M,Inoue S

    更新日期：2000-07-05 00:00:00

  • A novel firefly luciferase biosensor enhances the detection of apoptosis induced by ESAT-6 family proteins of Mycobacterium tuberculosis.

    abstract：:The activation of caspase-3 is a key surrogate marker for detecting apoptosis. To quantitate caspase-3 activity, we constructed a biosensor comprising a recombinant firefly luciferase containing a caspase-3 cleavage site. When apoptosis was induced, caspase-3 cleavage of the biosensor activated firefly luciferase by a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.09.047

    authors： Shi J,Zhang H,Fang L,Xi Y,Zhou Y,Luo R,Wang D,Xiao S,Chen H

    更新日期：2014-10-03 00:00:00

  • Comprehensive investigation of aberrant microRNAs expression in cells culture model of MnCl2-induced neurodegenerative disease.

    abstract：:Manganese (Mn) is required in various human physiological processes. Excessive Mn exposure causes manganism, a progressive neurodegenerative disorder similar to idiopathic Parkinson's disease (IPD). However, the detailed mechanism of Mn-induced neurotoxicity is not yet fully understood. MicroRNAs (miRNAs) play importa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.041

    authors： He R,Xie X,Lv L,Huang Y,Xia X,Chen X,Zhang L

    更新日期：2017-04-29 00:00:00

  • Drastic Ca2+ sensitization of myofilament associated with a small structural change in troponin I in inherited restrictive cardiomyopathy.

    abstract：:Six missense mutations in human cardiac troponin I (cTnI) were recently found to cause restrictive cardiomyopathy (RCM). We have bacterially expressed and purified these human cTnI mutants and examined their functional and structural consequences. Inserting the human cTnI into skinned cardiac muscle fibers showed that...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.116

    authors： Yumoto F,Lu QW,Morimoto S,Tanaka H,Kono N,Nagata K,Ojima T,Takahashi-Yanaga F,Miwa Y,Sasaguri T,Nishita K,Tanokura M,Ohtsuki I

    更新日期：2005-12-23 00:00:00

  • Alpha-lipoic acid reduces retinal cell death in diabetic mice.

    abstract：:Oxidative stress plays an important role in the development of diabetic retinopathy. Here, we examined whether α-lipoic acid (α-LA), a natural antioxidant, attenuated retinal injury in diabetic mice. The α-LA was orally administered to control mice or mice with streptozotocin-induced diabetes. We found that α-LA reduc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.07.041

    authors： Kim YS,Kim M,Choi MY,Lee DH,Roh GS,Kim HJ,Kang SS,Cho GJ,Hong EK,Choi WS

    更新日期：2018-09-10 00:00:00

  • Proliferation of hematopoietic cells is accompanied by suppressed expression of heat shock protein 70.

    abstract：:In this study, we have examined the synthesis of heat shock protein (HSP70) in leukemia cells from acute myelogenous leukemia (AML) patients and in mononuclear cells from normal individuals, before and after growth factor stimulation. We have shown that the HSP70 protein was expressed in these cells in the absence of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90544-u

    authors： Zhang W,Drach J,Andreeff M,Deisseroth A

    更新日期：1992-03-16 00:00:00

  • Physical association of Ras and cathepsins B and L in the conditioned medium of v-Ha-ras-transformed NIH3T3 cells.

    abstract：:Immunoblot analysis of the conditioned medium of v-Ha-ras-transformed NIH3T3 cells showed that Ras protein is secreted in the culture fluid. Because cathepsins L and B whose activities are inhibited by Ras are also secreted, possible association between Ras and cathepsins was investigated by immunoprecipitation and fo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2696

    authors： Hiwasa T,Kominami E

    更新日期：1995-11-22 00:00:00

  • Structure, chromosomal location, and expression profile of EXTR1 and EXTR2, new members of the multiple exostoses gene family.

    abstract：:Hereditary multiple exostoses (EXT) is an autosomal dominant disorder that is characterized by the appearance of multiple outgrowths of the long bones (exostoses) at their epiphyses. Genetical heterogeneities have segregated at least on chromosome 8, 11, and 19 and been designated EXT1, EXT2, and EXT3, respectively. R...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.8062

    authors： Saito T,Seki N,Yamauchi M,Tsuji S,Hayashi A,Kozuma S,Hori T

    更新日期：1998-02-04 00:00:00

  • Bisulfite conversion-specific and methylation-specific PCR: a sensitive technique for accurate evaluation of CpG methylation.

    abstract：:Methylation-specific PCR (MSP) is frequently used to distinguish methylated alleles in the genome. Sequences that have been incompletely converted during bisulfite treatment are frequently co-amplified during MSP. For accurate MSP, it is important to detect methylated sequences in a background of unconverted DNA with ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.08.005

    authors： Sasaki M,Anast J,Bassett W,Kawakami T,Sakuragi N,Dahiya R

    更新日期：2003-09-19 00:00:00

  • MiRNA-145 is involved in the development of resistin-induced insulin resistance in HepG2 cells.

    abstract：BACKGROUND:Resistin is associated with insulin resistance, and determining its developmental and molecular mechanisms may help the development of novel treatments. MicroRNAs (miRNAs) are involved in many physiological and pathological processes as negative regulators. However, it remains unclear whether miRNAs play a r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.02.034

    authors： Wen F,Yang Y,Jin D,Sun J,Yu X,Yang Z

    更新日期：2014-03-07 00:00:00

  • Wnt activator CHIR99021-stimulated human dermal papilla spheroids contribute to hair follicle formation and production of reconstituted follicle-enriched human skin.

    abstract：:The aim of the present study was to accomplish de novo generation of reconstituted human skin with enriched hair follicles. Dermal papillae (DP) are known to play a crucial organizing role in hair follicle induction. However, generation of enriched human hair follicles using cultured DP cells has not been accomplished...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.06.038

    authors： Yoshida Y,Soma T,Matsuzaki T,Kishimoto J

    更新日期：2019-08-27 00:00:00

  • Upregulation of myosin heavy chain MHClalpha in rat muscles after unweighting and clenbuterol treatment.

    abstract：:Myosin heavy chain (MHC) mRNA isoforms were quantified in soleus (SOL) and gastrocnemius (GAS) muscles from rats exposed to 14 days of either hindlimb unweighting (HU), clenbuterol treatment (CB), or HU combined with CB treatment (HU-CB). All conditions induced in SOL a shift from slow to faster MHC mRNA isoforms and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3305

    authors： Stevens L,Gohlsch B,Mounier Y,Pette D

    更新日期：2000-08-28 00:00:00

  • Presence of dioxins in human follicular fluid: their possible stage-specific action on the development of preimplantation mouse embryos.

    abstract：:Examination of human follicular fluid revealed the presence of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) at concentrations of approximately 1 pg/ml (0.01 pg TEQ/ml). To study their possible action, two-cell mouse embryos were cultured in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9340

    authors： Tsutsumi O,Uechi H,Sone H,Yonemoto J,Takai Y,Momoeda M,Tohyama C,Hashimoto S,Morita M,Taketani Y

    更新日期：1998-09-18 00:00:00

  • Interleukin 1 alpha mRNA in virus-transformed T and B cells.

    abstract：:IL-1 alpha cDNA clone was isolated from a T cell line infected by the human T lymphotropic retrovirus type-I (HTLV-I/ATLV). We found significant amounts of mRNA hybridizing to IL-1 alpha cDNA not only in HTLV-I-transformed T cells but also in Epstein-Barr Virus-transformed B cells. A part of IL-2 receptor inducing act...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80121-8

    authors： Noma T,Nakamura T,Maeda M,Okada M,Taniguchi Y,Tagaya Y,Yaoita Y,Yodoi J,Honjo T

    更新日期：1986-08-29 00:00:00

  • Localization of the dominant non-enzymatic intermolecular cross-linking sites on fibrous collagen.

    abstract：:Previous studies have shown that fibrous collagen undergoes intermolecular cross-linking at multiple sites of the elongated triple-helical regions among adjacent juxtaposed collagen molecules on incubation with a very high concentration of reducing sugar such as 200 mM ribose, and the similarity of the changes in its ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.04.011

    authors： Chiue H,Yamazoye T,Matsumura S

    更新日期：2015-06-05 00:00:00

  • CD99 mediates neutrophil transmigration through the bEnd.3 monolayer via the induction of oxygen-glucose deprivation.

    abstract：AIM/BACKGROUND:CD99 participate in neutrophil infiltration after inflammatory events; however, despite the important role of inflammation in ischemic stroke, the role of CD99 in ischemic stroke remains unclear. METHOD:In the present study, we detected the protein expression of CD99, ICAM-1, and CD31 (PECAM-1) in oxyge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.03.159

    authors： Hu T,Sun R,Huang F,Liu X,Duan Z,Ye R,Li Y,Xiao L,Guo Z,Liu Q,Zhu W

    更新日期：2020-06-04 00:00:00

  • Phorbol ester induces differential membrane-association of protein kinase C subspecies in human platelets.

    abstract：:Human platelets contained proteins which cross-reacted with antisera specific for brain protein kinase C-alpha and -beta. When platelets were incubated with 12-O-tetradecanoylphorbol-13-acetate there was a rapid accumulation of protein kinase C-alpha in the particulate fraction associated with a loss of this subspecie...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92635-1

    authors： Fournier A,Hardy SJ,Clark KJ,Murray AW

    更新日期：1989-06-15 00:00:00

  • Activation of membrane-bound phospholipase D by protein kinase C in HL60 cells: synergistic action of a small GTP-binding protein RhoA.

    abstract：:Regulation of phospholipase D (PLD) activation by protein kinase C (PKC) was studied in membranes isolated from human promyelocytic leukemia HL60 cells. The activation of membrane-bound PLD by PKC partially purified from rat brain was most effectively induced with phorbol 12-myristate 13-acetate (PMA) and Ca2+ (1 micr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1811

    authors： Ohguchi K,Banno Y,Nakashima S,Nozawa Y

    更新日期：1995-06-06 00:00:00

  • TFDP3 inhibits E2F1-induced, p53-mediated apoptosis.

    abstract：:By dimerizing with E2F proteins, TFDP has profound influence on cellular E2F activities. While TFDP1 and 2 enhance the DNA binding and the transcriptional activity of E2F, the newly identified member of the DP family, TFDP3 primarily functions as a negative regulator. To further characterize the inhibitory property of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.06.128

    authors： Tian C,Lv D,Qiao H,Zhang J,Yin YH,Qian XP,Wang YP,Zhang Y,Chen WF

    更新日期：2007-09-14 00:00:00

  • Targeting nanodisks via a single chain variable antibody--apolipoprotein chimera.

    abstract：:Nanodisks (ND) are nanometer scale complexes of phospholipid and apolipoprotein that have been shown to function as drug delivery vehicles. ND harboring significant quantities of the antifungal agent, amphotericin B, or the bioactive isoprenoid, all trans retinoic acid, have been generated and characterized. As curren...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.12.077

    authors： Iovannisci DM,Beckstead JA,Ryan RO

    更新日期：2009-02-06 00:00:00

  • The homology of complement factor C8 gamma chain and alpha-1-microglobulin.

    abstract：:The sequence of the complement factor C8 gamma chain shares a remarkable degree of similarity with that of alpha-1-microglobulin, a member of the alpha-2u-globulin superfamily. This superfamily comprises a diverse group of distantly related animal proteins possessing characteristic structural features and similar func...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91636-6

    authors： Hunt LT,Elzanowski A,Barker WC

    更新日期：1987-11-30 00:00:00

  • Advanced glomerulosclerosis is reversible in nephrotic mice.

    abstract：:Advanced glomerulosclerosis, a common hallmark of chronic renal diseases (CRD) is believed to be irreversible, and it is thought that glomerular hyperfiltration and hypertrophy may participate in its pathogenesis. We demonstrate here that glomerulosclerosis is "reversible" in an animal model. We used nephrotic ICGN (n...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4903

    authors： Mizuno-Horikawa Y,Mizuno S,Tamura S,Kurosawa T

    更新日期：2001-06-15 00:00:00

  • General order parameter based correlation analysis of protein backbone motions between experimental NMR relaxation measurements and molecular dynamics simulations.

    abstract：:Internal backbone dynamic motions are essential for different protein functions and occur on a wide range of time scales, from femtoseconds to seconds. Molecular dynamic (MD) simulations and nuclear magnetic resonance (NMR) spin relaxation measurements are valuable tools to gain access to fast (nanosecond) internal mo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.018

    authors： Liu Q,Shi C,Yu L,Zhang L,Xiong Y,Tian C

    更新日期：2015-02-13 00:00:00

  • Effects of the RNA-binding protein, KSRP, on innate immune response against Helicobacter pylori infection in mice.

    abstract：:Helicobacter pylori (H. pylori) contributes to various gastric diseases such as chronic gastritis, gastric ulcer, and gastric carcinoma. Host innate immune response against the pathogen plays a significant role in elimination of pathogen infection. Importantly, pathogen elimination is closely related to numerous infla...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.12.016

    authors： Li N,Cao M,Yi S,Cheng J,Wang L,Tao Y,Wu D,Peng J,Zhang M,Qi P,Zhao J

    更新日期：2018-01-08 00:00:00

  • Analysis of human terminal deoxynucleotidyl transferase cDNA expressible in mammalian cells.

    abstract：:Human Molt3 cDNA library was constructed using pcD vector system which permits the expression of cDNA inserts in mammalian cells. Nearly full-length human terminal deoxynucleotidyltransferase (TdT) cDNA was cloned using a fragment of bovine TdT cDNA as a probe. The human TdT cDNA contains an open reading frame of 1,55...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80493-x

    authors： Koiwai O,Kaneda T,Morishita R

    更新日期：1987-04-14 00:00:00

  • The fourth immunoglobulin-like loop in the extracellular domain of FLT-1, a VEGF receptor, includes a major heparin-binding site.

    abstract：:We found that heparin at low concentrations increases the vascular endothelial growth factor (VEGF)-induced proliferation of human umbilical vein endothelial cells (HUVECs) but at high concentrations decreases it. To examine whether FLT-1, a VEGF receptor, interacts with heparin and which domain of FLT-1 binds to hepa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1580

    authors： Park M,Lee ST

    更新日期：1999-11-02 00:00:00

  • Anti-insulin receptor serum mimics the developmental role of insulin in mouse mammary explants.

    abstract：:A number of growth factors can maintain hormonally-responsive epithelium in murine mammary explants as well as insulin, but only insulin can promote the synthesis of casein and alpha-lactalbumin, in the presence of glucocorticoid and prolactin. Anti-insulin receptor serum can elicit these effects of insulin on milk pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91397-9

    authors： Nicholas KR,Topper YJ

    更新日期：1983-03-29 00:00:00

  • Up-regulation of uncoupling protein-3 by fatty acid in C2C12 myotubes.

    abstract：:Uncoupling proteins (UCPs) are mitochondrial membrane proton transporters that uncouple oxidative phosphorylation by dissipating the proton gradient across the membrane. We have investigated regulation of the UCP3 gene in skeletal muscle and C2C12 muscle cells. UCP3 mRNA in mouse skeletal muscle is markedly increased ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0662

    authors： Hwang CS,Lane MD

    更新日期：1999-05-10 00:00:00