Abstract:
:Ceramide kinase (CERK) and its product, ceramide-1-phosphate (Cer-1-P), are implicated in signaling processes, but the action mechanisms are not fully elucidated. When checking for intracellular effects of Cer-1-P by exposing CERK-expressing CHO cells to truncated ceramides, an unexpected decrease in CERK activity and protein level was observed. This decrease appeared dose-dependent and specific for the d-erythro-ceramide configuration and the presence of the double bond. At early time points, CERK clustered near the plasma membrane, followed later by its appearance in the culture medium. In cells expressing CERK lacking the pleckstrin domain or an inactive CERK mutant, this ceramide effect was not observed, indicating that clustering and release of CERK may be mediated by Cer-1-P. Presumably, high local Cer-1-P concentrations will increase the plasma membrane curvature and lead to release of CERK by vesicle shedding. This could be a potential regulatory mechanism in CERK/Cer-1-P signaling so far not investigated.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Van Overloop H,Van Veldhoven PPdoi
10.1016/j.bbrc.2007.09.117subject
Has Abstractpub_date
2007-12-07 00:00:00pages
169-74issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(07)02091-8journal_volume
364pub_type
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