Abstract:
:We evaluated the role of apolipoprotein E (apoE) in the clearance of neutral and negatively charged liposomes by hepatocytes in apoE-deficient mice. Negatively charged liposomes were cleared at identical rates in apoE-deficient and wild-type mice; neutral liposomes were cleared at a 3.6-fold slower rate in apoE-deficient mice. ApoE deficiency did not affect hepatic uptake of negatively charged liposomes but lowered that of neutral liposomes >5-fold. Hepatocyte uptake of neutral liposomes was reduced >20-fold in apoE-deficient mice; that of negatively charged liposomes remained unchanged. We conclude that uptake of neutral liposomes by hepatocytes is nearly exclusively apoE-mediated.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Yan X,Kuipers F,Havekes LM,Havinga R,Dontje B,Poelstra K,Scherphof GL,Kamps JAdoi
10.1016/j.bbrc.2004.12.137subject
Has Abstractpub_date
2005-03-04 00:00:00pages
57-62issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(04)02954-7journal_volume
328pub_type
杂志文章abstract::We constructed a plasmid-based expression vector carrying the murine metallothionein gene promoter, the human preprorenin gene, the Tn5 neomycin phosphotransferase II gene, and a complete bovine papilloma virus genome. We were unsuccessful in cloning the preprorenin gene in E. coli when it was inserted into a plasmid ...
journal_title:Biochemical and biophysical research communications
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