Abstract:
:Understanding the pathogenesis of infectious diseases requires comprehensive knowledge of the proteins expressed by the pathogen during in vivo growth in the host. Proteomics provides the tools for such analyses but the protocols required to purify sufficient quantities of the pathogen from the host organism are currently lacking. Here, we present a rapid immunomagnetic protocol for the separation of Francisella tularensis, a highly virulent bacterium and potential biowarfare agent, from the spleens of infected mice. In less than one hour, bacteria can be isolated in quantities sufficient to carry out meaningful proteomic comparisons with in vitro grown bacteria. Furthermore, the isolates are virtually free from contaminating host proteins. Two-dimensional gel analysis revealed a host induced proteome in which 78 proteins were differentially expressed in comparison to in vitro grown controls. The results obtained clearly demonstrate the complexity of the adaptive response of F. tularensis to the host environment, and the difficulty of mimicking such behavior in vitro.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Twine SM,Mykytczuk NC,Petit MD,Shen H,Sjöstedt A,Wayne Conlan J,Kelly JFdoi
10.1016/j.bbrc.2006.05.070subject
Has Abstractpub_date
2006-07-14 00:00:00pages
1621-33issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(06)01128-4journal_volume
345pub_type
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journal_title:Biochemical and biophysical research communications
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