Site-directed mutagenesis of conserved residues of Clostridium thermocellum endoglucanase CelC.

abstract：:Bradykinin (BK) B2 receptor signaling involves activation of phospholipase C (PLC). PLC activation by other receptors consists of either allosteric activation of PLC beta isoforms by G-proteins or tyrosine phosphorylation of PLC gamma isoforms. Because the B2 receptor is a G-protein-coupled receptor, it has been assum...

journal_title：Biochemical and biophysical research communications

authors： Venema VJ,Ju H,Sun J,Eaton DC,Marrero MB,Venema RC

更新日期：1998-05-08 00:00:00

abstract：:Reactive oxygen species (ROS) are crucial secondary messengers of signaling pathways. Redox-dependent signaling events have been previously described in the innate immune response. However, the mechanism by which ROS modulates anti-viral innate immune signaling is not fully clarified. Here, we report that mitochondria...

journal_title：Biochemical and biophysical research communications

authors： Gonzalez-Dosal R,Horan KA,Paludan SR

更新日期：2012-02-24 00:00:00

abstract：:With the global pandemic of hepatitis B and C infections, the incidence of Hepatocellular carcinoma (HCC) is rapidly increasing world wide. We identified glypican-3 (GPC3), a novel oncofetal gene over-expressed specifically in human HCC, as based on data of cDNA microarrays. As GPC3 is a GPI-anchored membrane protein ...

journal_title：Biochemical and biophysical research communications

authors： Nakatsura T,Yoshitake Y,Senju S,Monji M,Komori H,Motomura Y,Hosaka S,Beppu T,Ishiko T,Kamohara H,Ashihara H,Katagiri T,Furukawa Y,Fujiyama S,Ogawa M,Nakamura Y,Nishimura Y

更新日期：2003-06-20 00:00:00

abstract：:One of the earliest events following stimulation of human platelets with thrombin is a rise in the cytosolic pH, pHi, mediated by Na+/H+ exchange, and an increase in the cytosolic free Ca2+ concentration, [Ca2+]i. In the present study we investigated whether an increase in pHi alone, induced by the Na+/H+ ionophore mo...

journal_title：Biochemical and biophysical research communications

authors： Siffert W,Akkerman JW

更新日期：1989-06-30 00:00:00

abstract：:A growing number of important regulatory proteins within cells are modified by conjugation of ubiquitin, a well-conserved 76-amino-acid polypeptide. The ubiquitinated proteins are targeted to proteasome for degradation or alternative metabolic fates, such as triggering of plasma membrane endocytosis and trafficking to...

journal_title：Biochemical and biophysical research communications

authors： Chung CH,Baek SH

更新日期：1999-12-29 00:00:00

abstract：:The effects of various growth factors on endothelial cell survival in vitro were studied. Using rat heart endothelial cells, the cell survival curves were obtained; the cells were cultured until confluent, the medium was changed to serum-free medium with or without growth factors, and the cells were counted after 3, 6...

journal_title：Biochemical and biophysical research communications

authors： Etoh T,Takehara K,Igarashi A,Ishibashi Y

更新日期：1989-08-15 00:00:00

abstract：:Rosiglitazone is a ligand of peroxisome proliferation-activated receptor gamma (PPARγ). However, it exerts biological activities and therapeutic effects through both PPARγ-dependent and independent mechanisms. In this study, we defined that rosiglitazone was also a ligand of retinoid X receptor alpha (RXRα) and displa...

journal_title：Biochemical and biophysical research communications

authors： Huang F,Li Y,Chen J,Zhang XK,Zhou H

更新日期：2020-09-10 00:00:00

abstract：:Treatment of rats with phenobarbital for three days greatly increases the activity of 2,5 oligoadenylate synthetase in liver nuclei. Analysis of 2',5'-oligoadenylates synthesized in vitro showed that nuclei from both phenobarbital-treated and control rats synthesized 2',5'-oligoadenylates ranging from di- to hexamers....

journal_title：Biochemical and biophysical research communications

authors： Liu DK,Owens GF

更新日期：1984-06-29 00:00:00

abstract：:The lep of Azotobacter vinelandii is an 852-base-pair open reading frame (ORF) which encodes a protein of 284 amino acid residues. The translated protein shares 75% homology with leader peptidase I isolated from Pseudomonas fluorescens and 37% homology with leader peptidase I isolated from Escherichia coli. Five highl...

journal_title：Biochemical and biophysical research communications

authors： Jock CA,Pulakat L,Lee S,Gavini N

更新日期：1997-10-20 00:00:00

abstract：:Lipoproteins are important biological components. However, they have few convenient fluorescent labelling probes currently reported, and their physiological reliability can be questioned. We compared the association of two fluorescent cholesterol derivatives, 22-nitrobenzoxadiazole-cholesterol (NBD-Chol) and 21-methyl...

journal_title：Biochemical and biophysical research communications

authors： Gaibelet G,Tercé F,Bertrand-Michel J,Allart S,Azalbert V,Lecompte MF,Collet X,Orlowski S

更新日期：2013-11-01 00:00:00

abstract：:The sulfhydryl group reagent N-ethylmaleimide was found to inhibit in a dose dependent manner regulatory volume decrease of human peripheral lymphocytes swollen in buffered hyposmotic NaCl media. In hyposmotic KCl media NEM treated lymphocytes prevented an additional secondary swelling seen in control lymphocytes. The...

journal_title：Biochemical and biophysical research communications

authors： Bauer J,Lauf PK

更新日期：1983-11-30 00:00:00

abstract：:A chemical inhibitor library of 84 compounds was screened to investigate the signaling pathway(s) leading to activation of Nrf2 in response to nitric oxide (NO). We identified the protein kinase C delta (PKCdelta) inhibitor rottlerin as the only compound that reduced NO-induced ARE-luciferase reporter activity and dim...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Hung AC,Ng PY,Nakayama K,Hu Y,Li B,Porter AG,Dhakshinamoorthy S

更新日期：2009-09-04 00:00:00

abstract：:The presence and localization of mitochondrial calmodulin was directly proved immuno-electron microscopically by the protein A-gold technique. In the ultra-pure mitochondria the complexes of anti-calmodulin antibody and protein A-gold clearly showed the localization of mitochondrial calmodulin on the inner membrane an...

journal_title：Biochemical and biophysical research communications

authors： Hatase O,Doi A,Itano T,Matsui H,Ohmura Y

更新日期：1985-10-15 00:00:00

abstract：:Autophagy is the bulk degradation of cytosolic materials in lysosomes/vacuoles of eukaryotic cells. In the yeast Saccharomyces cerevisiae, 17 Atg proteins are known to be involved in autophagosome formation. Genome wide analyses have shown that Atg17 interacts with numerous proteins. Further studies on these interacti...

journal_title：Biochemical and biophysical research communications

authors： Kabeya Y,Kawamata T,Suzuki K,Ohsumi Y

更新日期：2007-05-04 00:00:00

abstract：:We describe the isolation of a synthetic agonist of IFN-gamma (L-mIFN-gamma-CT) active on mouse and human cells. Its biological activity is the result of the ability of the C-terminal extremity of murine IFN-gamma to interact with the intracellular part of IFNgamma-R and the observation that the modification of peptid...

journal_title：Biochemical and biophysical research communications

authors： Thiam K,Loing E,Delanoye A,Diesis E,Gras-Masse H,Auriault C,Verwaerde C

更新日期：1998-12-30 00:00:00

abstract：:Protein phosphatase 2Cs (PP2Cs) have been referred to act as negative modulators of the protein kinase pathways involved in different environmental stress responses and developmental processes. In Arabidopsis, PP2Cs have been extensively studied and some are known to negatively regulate abscisic acid signaling. In ric...

journal_title：Biochemical and biophysical research communications

authors： Amir Hossain M,Ahn CH,Lee IS,Jeon JS,An G,Park PB

更新日期：2018-09-10 00:00:00

abstract：:We have recently found that 3T3-L1 adipocytes secrete microvesicles, known as adipocyte-derived microvesicles (ADMs), with angiogenic activity. In this study, we found that ADMs contain RNA without typical 28S and 18S ribosomal RNA inside the vesicles. Microarray analysis revealed that ADMs contain approximately 7000 ...

journal_title：Biochemical and biophysical research communications

authors： Ogawa R,Tanaka C,Sato M,Nagasaki H,Sugimura K,Okumura K,Nakagawa Y,Aoki N

更新日期：2010-08-06 00:00:00

abstract：:Recent studies from our laboratory demonstrated that Egr-1 is upregulated in the rat duodenal mucosa during cysteamine-induced duodenal ulceration and that antisense egr-1 oligonucleotide aggravates the duodenal ulcers. This study was aimed to determine the effects of cysteamine on redox-sensitive Egr-1 transcriptiona...

journal_title：Biochemical and biophysical research communications

authors： Khomenko T,Deng X,Jadus MR,Szabo S

更新日期：2003-10-03 00:00:00

abstract：:Despite the central role of proteasomes in relevant physiological pathways and pathological processes, this topic is unexpectedly largely unexplored in human liver. Here we present data on the presence of proteasome and immunoproteasome in human livers from normal adults, fetuses and patients affected by major hepatic...

journal_title：Biochemical and biophysical research communications

authors： Vasuri F,Capizzi E,Bellavista E,Mishto M,Santoro A,Fiorentino M,Capri M,Cescon M,Grazi GL,Grigioni WF,D'Errico-Grigioni A,Franceschi C

更新日期：2010-06-25 00:00:00

abstract：:The flavanol (-)-epicatechin has been found to protect against damage inflicted by peroxynitrite, an inflammatory intermediate. Here, epicatechin was tested in systems of increasing complexity. The compound efficiently protected against nitration of protein tyrosine residues by peroxynitrite (IC(50) approximately 0.02...

journal_title：Biochemical and biophysical research communications

authors： Schroeder P,Klotz LO,Buchczyk DP,Sadik CD,Schewe T,Sies H

更新日期：2001-07-20 00:00:00

abstract：:In a number of cell lines responsive to basic fibroblast growth factor (bFGF), two major tyrosine phosphorylated proteins, of molecular weights around 120kDa and 90kDa, are precipitated along with the tyrosine phosphatase SHP2 from the lysates of stimulated cells. The docker protein Gab-1 represents at least part of t...

journal_title：Biochemical and biophysical research communications

authors： Ong SH,Lim YP,Low BC,Guy GR

更新日期：1997-09-08 00:00:00

abstract：:Comparative fluorimetric studies on the long-term (8-hour) action of beta[1-42]amyloid and its shorter fragments beta[1-40], beta[25-35] and beta[31-35] on the steady-state intracellular Ca2+ concentration in primary cultures of rat astroglial cells using the Ca2+-sensitive fluorescent probe Fura-2 AM revealed higher ...

journal_title：Biochemical and biophysical research communications

authors： Laskay G,Zarándi M,Varga J,Jost K,Fónagy A,Torday C,Latzkovits L,Penke B

更新日期：1997-06-27 00:00:00

abstract：:Fecal supernatant or serum containing TT virus (TTV) of genotype 1a (10(5) copies/ml) from patients with acute TTV infection was inoculated intravenously into two naive chimpanzees. Serum samples were obtained weekly and tested for TTV DNA by genotype 1-specific polymerase chain reaction. TTV DNA was detected in chimp...

journal_title：Biochemical and biophysical research communications

authors： Tawara A,Akahane Y,Takahashi M,Nishizawa T,Ishikawa T,Okamoto H

更新日期：2000-11-19 00:00:00

abstract：:When TT virus (TTV) DNA was quantitated in whole blood and plasma aliquots from 27 viremic individuals by real-time detection PCR that can detect essentially all TTV genotypes, the TTV load was 6.9 +/- 3.5 (mean +/- standard deviation)-fold higher in the whole blood than in the plasma samples [P < 0.002 (paired t test...

journal_title：Biochemical and biophysical research communications

authors： Takahashi M,Asabe S,Gotanda Y,Kishimoto J,Tsuda F,Okamoto H

更新日期：2002-01-11 00:00:00

abstract：:X-ray microanalysis has been used to determine elemental content in the cytoplasm of hepatocytes of hyperthyroid rats in comparison with euthyroid controls. No significant differences were found for any examined element (sodium, phosphorus, sulfur, chlorine, potassium, magnesium and calcium). In contrast to earlier re...

journal_title：Biochemical and biophysical research communications

authors： Smith NK,Lewiński A,Stabler-Morris S

更新日期：1983-08-30 00:00:00

abstract：:Pentamannose 6-phosphate/trilysine substituted aprotinin (PMP-lys-aprotinin) and insulin like growth factor II (IGF II) were used as affinity ligands for the mannose 6-phosphate (M6P) and IGF II binding sites of the M6P/IGF II receptor. Both ligands were cross linked to intact receptor and tryptic fragments of the rec...

journal_title：Biochemical and biophysical research communications

authors： Waheed A,Braulke T,Junghans U,von Figura K

更新日期：1988-05-16 00:00:00

abstract：:Glioma is one of the most common primary brain tumors in adults with a high mortality rate and relapse rate. Thus, finding better effective approaches to treat glioma has become very urgent. Astragaloside IV (AS-IV), the major active triterpenoid in Radix Astragali, has shown anti-tumorigenic properties in certain can...

journal_title：Biochemical and biophysical research communications

authors： Li B,Wang F,Liu N,Shen W,Huang T

更新日期：2017-09-09 00:00:00

abstract：:Glycoprotein130 (gp130) is an important signal transducer in the interleukin-6 (IL-6) trans-signaling pathway, which plays a crucial role in chronic inflammation in atherosclerosis. Studies suggest that estrogen can inhibit IL-6/gp130 signaling and reduce the risk of coronary artery disease, but the precise mechanism ...

journal_title：Biochemical and biophysical research communications

authors： Zhou M,Dai W,Cui Y,Li Y

更新日期：2020-03-12 00:00:00

abstract：:The CTCF-like protein, CTCFL, is a DNA-binding factor that regulates the transcriptional program of mammalian male germ cells. CTCFL consists of eleven zinc fingers flanked by polypeptides of unknown structure and function. We determined that the C-terminal fragment predominantly consists of extended and unordered con...

journal_title：Biochemical and biophysical research communications

authors： Campbell AE,Martinez SR,Miranda JJ

更新日期：2010-06-04 00:00:00

abstract：:Structural maintenance of chromosome 1 (Smc1) is a multifunctional protein, which has been implicated in sister chromatid cohesion, DNA recombination and repair, and the activation of cell cycle checkpoints by ionizing radiation, ultraviolet light, and other genotoxic agents. In order to identify the proteins that int...

journal_title：Biochemical and biophysical research communications

authors： Ryu MJ,Kim BJ,Lee JW,Lee MW,Choi HK,Kim ST

更新日期：2006-03-17 00:00:00