当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Nucleotide sequence and genetic complementation analysis of lep from Azotobacter vinelandii.

Nucleotide sequence and genetic complementation analysis of lep from Azotobacter vinelandii.

Abstract：

:The lep of Azotobacter vinelandii is an 852-base-pair open reading frame (ORF) which encodes a protein of 284 amino acid residues. The translated protein shares 75% homology with leader peptidase I isolated from Pseudomonas fluorescens and 37% homology with leader peptidase I isolated from Escherichia coli. Five highly conserved regions found in the family of leader peptidase I proteins are conserved in A. vinelandii Lep. The putative membrane topology of the protein seems similar to that of E. coli leader peptidase I based on the hydrophobicity analysis of the predicted amino acid sequence. Southern blotting analysis of the A. vinelandii chromosome by probing with lep specific DNA revealed that lep is present as a single copy per the chromosome. A multicopy plasmid carrying A. vinelandii lep could complement a temperature sensitive lep mutant of E. coli strain IT41, suggesting that we have identified the functional copy of lep present on A. vinelandii genome.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Jock CA,Pulakat L,Lee S,Gavini N

doi

10.1006/bbrc.1997.7452

subject

Has Abstract

pub_date

1997-10-20 00:00:00

pages

393-400

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(97)97452-0

journal_volume

239

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Functional characterization of Brassica napus DNA topoisomerase Iα-1 and its effect on flowering time when expressed in Arabidopsis thaliana.

    abstract：:Previous studies have shown that DNA topoisomerase Iα (AtTOP1α) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.011

    authors： Gao C,Qi S,Liu K,Li D,Jin C,Duan S,Zhang M,Chen M

    更新日期：2017-04-22 00:00:00

  • Hepatitis B virus X protein mutants exhibit distinct biological activities in hepatoma Huh7 cells.

    abstract：:The role of the hepatitis B virus X protein (HBx) in hepatocarcinogenesis remains controversial. To investigate the biological impact of hepatitis B virus x gene (HBx) mutation on hepatoma cells, plasmids expressing the full-length HBx or HBx deletion mutants were constructed. The biological activities in these transf...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.087

    authors： Liu X,Zhang S,Lin J,Zhang S,Feitelson MA,Gao H,Zhu M

    更新日期：2008-09-05 00:00:00

  • Bitter taste receptor T2R1 activities were compatible with behavioral sensitivity to bitterness in chickens.

    abstract：:Clarification of the mechanism of the sense of taste in chickens will provide information useful for creating and improving new feedstuffs for chickens, because the character of the taste receptors in oral tissues affects feeding behavior in animals. In this study, we focused on the sensitivity to bitterness in chicke...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.056

    authors： Hirose N,Kawabata Y,Kawabata F,Nishimura S,Tabata S

    更新日期：2015-05-01 00:00:00

  • Bile acids of patients with renal failure (possibility of bile acid secretion in the distal tubule).

    abstract：:Bile acids of patients with renal failure and of healthy subjects were analyzed by capillary gas chromatography after group separation. The amount of bile acids in the total dialysate (150 L) of the patients was smaller than that in the 24-h urine of healthy subjects. Polar bile acid sulfates constituted 17.3% and 30....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80699-5

    authors： Yamaga N,Adachi K,Kohara H,Shimizu K

    更新日期：1988-04-15 00:00:00

  • Reaction of optically active S- and R-forms of dolichyl phosphates with activated sugars.

    abstract：:Chemical synthesis was used to produce optically active isomers of dolichol (S- and R-forms) with 18 and 19 isoprene residues. The phosphorylated polyprene was studied in rat liver microsomal GDP-mannosyl and UDP-N-acetylglucosaminyl transferase systems. The two dolichol-P forms in both transferase systems gave Vmax v...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90439-5

    authors： Löw P,Peterson E,Mizuno M,Takigawa T,Chojnacki T,Dallner G

    更新日期：1985-07-16 00:00:00

  • Sulfated oxysterol, 25HC3S, is a potent regulator of lipid metabolism in human hepatocytes.

    abstract：:Recently, a novel oxysterol, 5-cholesten-3beta, 25-diol 3-sulfate (25HC3S) was identified in primary rat hepatocytes following overexpression of the cholesterol transport protein, StarD1. This oxysterol was also detected in human liver nuclei. In the present study, 25HC3S was chemically synthesized. Addition of 25HC3S...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.06.143

    authors： Ren S,Li X,Rodriguez-Agudo D,Gil G,Hylemon P,Pandak WM

    更新日期：2007-09-07 00:00:00

  • Polymorphism in the second intron of dopamine receptor D4 gene in humans and apes.

    abstract：:The dopamine receptor D4 (DRD4) has received increasing research attention in behavioral science, psychiatry, and psychopharmacology. However, the number of available genetic markers for primates is still insufficient. We identified a novel variation/polymorphism in the second intron of DRD4 in humans based on the sur...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.006

    authors： Shimada MK,Inoue-Murayama M,Ueda Y,Maejima M,Murayama Y,Takenaka O,Hayasaka I,Ito S

    更新日期：2004-04-16 00:00:00

  • Transforming growth factor-beta1 induces matrix metalloproteinase-9 expression in human meningeal cells via ERK and Smad pathways.

    abstract：:Transforming growth factor (TGF)-beta1, a cytokine released into the cerebrospinal fluid (CSF) after intraventricular hemorrhage (IVH), stimulates the expression of the components of the extracellular matrix (ECM), which causes progressive ventricular dilatation by impaired CSF absorption. Matrix metalloproteinase-9 (...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.038

    authors： Okamoto T,Takahashi S,Nakamura E,Nagaya K,Hayashi T,Fujieda K

    更新日期：2009-06-12 00:00:00

  • Human serum immunoglobulin G3 subclass bound preferentially to asialo-, agalactoimmunoglobulin A1/Sepharose.

    abstract：:As we reported before, exoglycosidase treatment of human serum IgA1 changed it to a sticky molecule. In order to examine the presence of the specific binding protein to the sticky IgA1 in human serum, IgA1, asialo-IgA1 (IgA1-S) and asialo-, agalacto-IgA1 (IgA1-SG)/Sepharose column chromatography of normal human serum ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1369

    authors： Iwase H,Yokozeki Y,Hiki Y,Tanaka A,Kokubo T,Sano T,Ishii-Karakasa I,Hisatani K,Kobayashi Y,Hotta K

    更新日期：1999-10-22 00:00:00

  • Immunoreactivity and proliferative actions of beta 2 microglobulin on human bone-derived cells in vitro.

    abstract：:Recent studies have demonstrated homology between bone-derived growth factor and beta 2 microglobulin. We have shown that beta 2 microglobulin has proliferative actions on human bone-derived cells in vitro and that these cells also show immunogenicity for beta 2 microglobulin. beta 2 microglobulin stimulated the incor...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91635-p

    authors： Evans DB,Thavarajah M,Kanis JA

    更新日期：1991-03-29 00:00:00

  • Glypican-3, overexpressed specifically in human hepatocellular carcinoma, is a novel tumor marker.

    abstract：:With the global pandemic of hepatitis B and C infections, the incidence of Hepatocellular carcinoma (HCC) is rapidly increasing world wide. We identified glypican-3 (GPC3), a novel oncofetal gene over-expressed specifically in human HCC, as based on data of cDNA microarrays. As GPC3 is a GPI-anchored membrane protein ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00908-2

    authors： Nakatsura T,Yoshitake Y,Senju S,Monji M,Komori H,Motomura Y,Hosaka S,Beppu T,Ishiko T,Kamohara H,Ashihara H,Katagiri T,Furukawa Y,Fujiyama S,Ogawa M,Nakamura Y,Nishimura Y

    更新日期：2003-06-20 00:00:00

  • Thiol levels in CD134-defined subsets of rat T lymphocytes: possible implications for HgCl2-induced immune dysregulation.

    abstract：:CD134 (OX40), a member of the tumour necrosis factor receptor family, is expressed on activated T cells and mediates T and B cell costimulation. Its expression is increased after exposure to the thiol-binding compound HgCl2 in BN rats, but not in Lewis rats, in association with induction of a T cell-dependent systemic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7679

    authors： Roos A,Claessen N,Schilder-Tol EJ,Chand MA,Weening JJ,Aten J

    更新日期：1997-11-17 00:00:00

  • Evidence for catalytic cysteine-histidine dyad in chalcone synthase.

    abstract：:Chalcone and stilbene synthases (CHS and STS) catalyze condensation reactions of p-coumaroyl-CoA and three C(2)-units from malonyl-CoA, but catalyze different cyclization reactions to produce naringenin chalcone and resveratrol, respectively. Condensing activities of wild-type CHS and STS as well as STS-C60S mutant we...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3368

    authors： Suh DY,Kagami J,Fukuma K,Sankawa U

    更新日期：2000-09-07 00:00:00

  • Abnormal activation of K+ channels underlies relaxation to bacterial lipopolysaccharide in rat aorta.

    abstract：:We have examined the role of K+ channels in mediating vasorelaxation produced by bacterial lipopolysaccharide (LPS) in endothelial-denuded strips of rat aorta precontracted with phenylephrine (1 microM). Salmonella typhosa LPS (0.1 microgram/ml) caused significant relaxation of tension which peaked at approximately 4h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1005

    authors： Hall S,Turcato S,Clapp L

    更新日期：1996-07-05 00:00:00

  • The enhanced IL-18 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT).

    abstract：:Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and hu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02433-6

    authors： Cho D,Seung Kang J,Hoon Park J,Kim YI,Hahm E,Lee J,Yang Y,Jeon J,Song H,Park H,Kim T,Pang S,Kim CW,Il Hwang Y,Jae Lee W

    更新日期：2002-10-25 00:00:00

  • The phorbol ester, 12-O-tetradecanoylphorbol-13-acetate enhances the long-term maintenance of pancreatic islet B cell differentiated function in tissue culture.

    abstract：:A study was undertaken to examine the effects of the tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate, on pancreatic islet B cell differentiated function and number. Islets from 3-day-old rats were maintained in tissue culture for up to 30 days with or without the addition of the phorbol ester. Chan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)91084-8

    authors： Schwizer RW

    更新日期：1985-05-16 00:00:00

  • MicroRNA-224 targets RKIP to control cell invasion and expression of metastasis genes in human breast cancer cells.

    abstract：:The Raf kinase inhibitor protein (RKIP) is a tumor suppressor that protects against metastasis and genomic instability. RKIP is downregulated in many types of tumors, although the mechanism for this remains unknown. MicroRNAs silence target genes via translational inhibition or target mRNA degradation, and are thus im...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.07.025

    authors： Huang L,Dai T,Lin X,Zhao X,Chen X,Wang C,Li X,Shen H,Wang X

    更新日期：2012-08-24 00:00:00

  • Inorganic phosphate regulates CryIVA protoxin expression in Bacillus thuringiensis israelensis.

    abstract：:The role of nutritional factors during CryIVA protoxin expression in Bacillus thuringiensis israelensis (Bti) has been investigated. Inorganic phosphate (Pi) was found to stimulate 135 kD protoxin synthesis by Bti cells. There was a corresponding increase in the cryIVA specific mRNA in the presence of Pi. Inorganic ph...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1094

    authors： Banerjee-Bhatnagar N

    更新日期：1999-08-27 00:00:00

  • Molecular characterization of seizure-related genes isolated by differential screening.

    abstract：:To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confirmed the results of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0313

    authors： Kajiwara K,Nagawawa H,Shimizu-Nishikawa S,Ookuri T,Kimura M,Sugaya E

    更新日期：1996-02-27 00:00:00

  • Expression profile analysis of colon cancer cells in response to sulindac or aspirin.

    abstract：:Nonsteroidal anti-inflammatory drugs (NSAIDs) have a preventive effect against colorectal cancer. Although inhibition of cyclooxygenase-2 plays a crucial role in the suppression of tumors, precise mechanisms of their action remain to be disclosed. To identify genes involved in the growth-suppressive effect of NSAIDs, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6648

    authors： Iizaka M,Furukawa Y,Tsunoda T,Akashi H,Ogawa M,Nakamura Y

    更新日期：2002-03-29 00:00:00

  • Effects of C-reactive protein on atherogenic mediators and adrenomedullin in human coronary artery endothelial and smooth muscle cells.

    abstract：:We examined the effects of recombinant human C-reactive protein (rhCRP) on atherosclerosis-related factors in cultured human coronary artery endothelial and smooth muscle cells (HCAECs and HCASMCs). After removing endotoxin from commercial rhCRP preparations using the appropriate column, the purified (P)-rhCRP retaine...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.004

    authors： Nagoshi Y,Kuwasako K,Cao YN,Kitamura K,Eto T

    更新日期：2004-02-20 00:00:00

  • GOLGA2 loss causes fibrosis with autophagy in the mouse lung and liver.

    abstract：:Autophagy is a biological recycling process via the self-digestion of organelles, proteins, and lipids for energy-consuming differentiation and homeostasis. The Golgi serves as a donor of the double-membraned phagophore for autophagosome assembly. In addition, recent studies have demonstrated that pulmonary and hepati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.049

    authors： Park S,Kim S,Kim MJ,Hong Y,Lee AY,Lee H,Tran Q,Kim M,Cho H,Park J,Kim KP,Park J,Cho MH

    更新日期：2018-01-01 00:00:00

  • Are hypothalamic neurons transsynaptically connected to porcine adipose tissue?

    abstract：:Specific anatomical sites and pathways responsible for mediating metabolic and neuroendocrine effects of leptin are still poorly understood. Therefore, we examined distribution of leptin receptor-containing neurons transsynaptically connected with the porcine fat tissue by means of combined viral transneuronal tracing...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.10.026

    authors： Czaja K,Kraeling RR,Barb CR

    更新日期：2003-11-14 00:00:00

  • CCAAT/enhancer-binding protein CEBP-2 controls fat consumption and fatty acid desaturation in Caenorhabditis elegans.

    abstract：:Mammalian CCAAT/enhancer-binding proteins (C/EBPs) are generally known as regulators in adipocyte differentiation. However, more understanding of the role of C/EBPs in lipid and glucose metabolism remains to be discovered. In this study, we verified the effect of CEBP-2, the homolog of CEBPs, on fat storage in Caenorh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.106

    authors： Xu XY,Hu JP,Wu MM,Wang LS,Fang NY

    更新日期：2015-12-04 00:00:00

  • Spore-displayed streptavidin: a live diagnostic tool in biotechnology.

    abstract：:Streptavidin, which is one of the most widely used proteins in biotechnological application field and is active only in tetrameric form, was surface expressed on the surface of Bacillus subtilis spore. Spore coat protein of B. subtilis, CotG, was used as an anchoring motif to display streptavidin. FACS using anti-stre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.144

    authors： Kim JH,Lee CS,Kim BG

    更新日期：2005-05-27 00:00:00

  • Connexin 30.2 is expressed in mouse pancreatic beta cells.

    abstract：:Nowadays, connexin (Cx) 36 is considered the sole gap junction protein expressed in pancreatic beta cells. In the present research we investigated the expression of Cx30.2 mRNA and protein in mouse pancreatic islets. Cx30.2 mRNA and protein were identified in isolated islet preparations by qRT-PCR and Western blot, re...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.06.100

    authors： Coronel-Cruz C,Hernández-Tellez B,López-Vancell R,López-Vidal Y,Berumen J,Castell A,Pérez-Armendariz EM

    更新日期：2013-09-06 00:00:00

  • The effect of phytochrome action on the activity of cytosolic cholinesterase in oat cells.

    abstract：:Cholinesterases in the oat cell were found to be distributed in the cell wall (50%) and cytoplasm (42%). Activity of the cytosolic enzyme was inhibited about 80% by 1 mM Ca2+. The enzyme activity was also inhibited by Mn2+, but no inhibition by Mg2+ was observed. Effects of red light and calcium ion on the enzyme acti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91448-2

    authors： Kim HY,Kim TI,Kim HK,Chae Q

    更新日期：1990-05-31 00:00:00

  • Structural characteristics of immunogenic liver-stage antigens derived from P. falciparum malarial proteins.

    abstract：:A fully effective antimalarial vaccine must contain multiple proteins from the different development stages of Plasmodium falciparum parasites involved in host-cell invasion or their biologically active fragments. It must therefore include sporozoite molecules able to induce protective immunity by blocking the parasit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.138

    authors： Cifuentes G,Vanegas M,Martinez NL,Pirajan C,Patarroyo ME

    更新日期：2009-07-10 00:00:00

  • Rare codon priority and its position specificity at the 5' of the gene modulates heterologous protein expression in Escherichia coli.

    abstract：:Rare codons and their effects in heterologous protein expression in Escherichia coli were addressed by many investigators. Here, we propose that not all rare codons of a foreign gene have negative effect but selective codon among them and its specific position in the downstream of the start codon modulates the express...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.09.024

    authors： Thangadurai C,Suthakaran P,Barfal P,Anandaraj B,Pradhan SN,Ramalingam S,Murugan V

    更新日期：2008-11-28 00:00:00

  • Oligonucleotide-capped gold nanoparticles for improved atomic force microscopic imaging and enhanced selectivity in polynucleotide detection.

    abstract：:A novel assay for selective determination of polynucleotides using atomic force microscopy in conjunction with the formation of the probe/target/DNA-gold nanoparticle sandwich structure at a gold surface is described. A 17-mer probe was attached to the surface for subsequent hybridization with a polynucleotide target....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3943

    authors： Han S,Lin J,Zhou F,Vellanoweth RL

    更新日期：2000-12-09 00:00:00