Hepatitis B virus X protein mutants exhibit distinct biological activities in hepatoma Huh7 cells.

abstract：:In this study, we devised a simple and rapid method to analyze fidelity of reverse transcriptase (RT) using next-generation sequencing (NGS). The method comprises a cDNA synthesis reaction from standard RNA with a primer containing a tag of 14 randomized bases and the RT to be tested, PCR using high-fidelity DNA polym...

journal_title：Biochemical and biophysical research communications

authors： Yasukawa K,Iida K,Okano H,Hidese R,Baba M,Yanagihara I,Kojima K,Takita T,Fujiwara S

更新日期：2017-10-14 00:00:00

abstract：:We isolated mouse CPP32/apopain cDNA, a mammalian homologue most closely related to Ced-3 in C. elegans, and examined the involvement of CPP32 in the apoptosis of nervous system during development. CPP32 is specifically expressed in the trigeminal (V) ganglia, facio-acoustic (VII-VIII) ganglion complex, and dorsal roo...

journal_title：Biochemical and biophysical research communications

authors： Mukasa T,Urase K,Momoi MY,Kimura I,Momoi T

更新日期：1997-02-24 00:00:00

abstract：:Lung cancer is a major cause of cancer-related mortality in the United States and around the world. Due to the pre-existing or acquired chemo-resistance, the current standard chemotherapy regimens only show moderate activity against lung cancer. In the current study, we explored the potential anti-lung cancer activity...

journal_title：Biochemical and biophysical research communications

authors： Kai S,Lu JH,Hui PP,Zhao H

更新日期：2014-09-26 00:00:00

abstract：:Fluorescent ubiquitination-based cell cycle indicator (Fucci) technology utilizing the cell cycle-dependent proteolysis of ubiquitin oscillators enables visualization of cell cycle progression in living cells. The Fucci probe consists of two chimeric fluorescent proteins, FucciS/G2/M and FucciG1, which label the nucle...

journal_title：Biochemical and biophysical research communications

authors： Yo M,Sakaue-Sawano A,Noda S,Miyawaki A,Miyoshi H

更新日期：2015-01-30 00:00:00

abstract：:Neurogenin-3 (ngn-3) expression is critical for endocrine development in the developing pancreas. We found that when ngn-3 was inhibited in an E11.5 pancreas, using either morpholino antisense or siRNA, it led to a significant decrease in endocrine differentiation after seven days in culture. Endocrine differentiation...

journal_title：Biochemical and biophysical research communications

authors： Prasadan K,Tulachan S,Guo P,Shiota C,Shah S,Gittes G

更新日期：2010-06-11 00:00:00

abstract：:Precise control of neuronal migration is essential for the development of the neocortex. However, the molecular mechanisms underlying neuronal migration remain largely unknown. Here we identified helix-loop-helix transcription factor Ebf3 as a Prdm8 target gene, and found that Ebf3 is a key regulator of neuronal migra...

journal_title：Biochemical and biophysical research communications

authors： Iwai R,Tabata H,Inoue M,Nomura KI,Okamoto T,Ichihashi M,Nagata KI,Mizutani KI

更新日期：2018-01-01 00:00:00

abstract：:N-Palmitoyl-(S)-seryl-(S)-seryl-(S)-asparaginyl-(S)-alanine (Palmitoyl-tetrapeptide) is an analogue of the N-terminal part of the lipoprotein from the outer membrane of Escherichia coli. It was prepared by chemical synthesis and tested for biological activity in in vitro lymphocyte culture systems. In spleen cells of ...

journal_title：Biochemical and biophysical research communications

authors： Bessler WG,Cox M,Wiesmüller KH,Jung G

更新日期：1984-05-31 00:00:00

abstract：:gamma S-Crystallin from shark eye lenses, formerly termed beta s crystallin in mammalian lenses, is structurally characterized in this study by cDNA cloning and sequencing. To facilitate sequence characterization of gamma S-crystallin possessing intermediate structural properties between beta- and gamma-crystallins, c...

journal_title：Biochemical and biophysical research communications

authors： Pan FM,Chuang MH,Chiou SH

更新日期：1997-11-07 00:00:00

abstract：:Tissue factor (TF) is an initiation factor for blood coagulation and its expression is induced on endothelial cells during inflammatory or immune responses. We designed an antisense oligodeoxynucleotide (AS-1/TF) for rat TF and studied its effect on hepatic ischemic reperfusion injury. AS-1/TF was delivered intravenou...

journal_title：Biochemical and biophysical research communications

authors： Nakamura K,Kadotani Y,Ushigome H,Akioka K,Okamoto M,Ohmori Y,Yaoi T,Fushiki S,Yoshimura R,Yoshimura N

更新日期：2002-09-27 00:00:00

abstract：:Incubation of purified bovine photoreceptor rod outer segments with [gamma-32P]ATP resulted in the labeling of phosphatidylinositol 4-phosphate (PIP) and phosphatidic acid (PA) with little labeling of phosphatidylinositol 4,5-bisphosphate (PIP2). Propranolol inhibited in a dose-dependent manner the labeling of PA and ...

journal_title：Biochemical and biophysical research communications

authors： Van Rooijen LA,Bazan NG

更新日期：1986-01-14 00:00:00

abstract：:MOTS-c, as a mitochondria derived peptide, exerts benefits for insulin resistance in HFD mice and against various stresses in an AMPK dependent way. Here, in the D-galactose chronic injection models, exogenous MOTS-c was given to determine its direct anti-aging effects. The body weight, insulin sensitivity and blood g...

journal_title：Biochemical and biophysical research communications

authors： Li Q,Lu H,Hu G,Ye Z,Zhai D,Yan Z,Wang L,Xiang A,Lu Z

更新日期：2019-05-28 00:00:00

abstract：:Chondromodulin-I (ChM-I) is a cartilage-specific glycoprotein that stimulates the growth of chondrocytes and inhibits the tube formation of endothelial cells. In the present study, we identified a novel ChM-I like molecule, designated ChM1L. Cloning of full length cDNAs of human, mouse, and rat ChM1L revealed that ChM...

journal_title：Biochemical and biophysical research communications

authors： Yamana K,Wada H,Takahashi Y,Sato H,Kasahara Y,Kiyoki M

更新日期：2001-02-02 00:00:00

abstract：:Multidrug resistance protein 1 (MRP1/ABCC1) is a 190kDa member of the ATP-binding cassette (ABC) superfamily of transmembrane transporters that is clinically relevant for its ability to confer multidrug resistance by actively effluxing anticancer drugs. Knowledge of the atomic structure of MRP1 is needed to elucidate ...

journal_title：Biochemical and biophysical research communications

authors： DeGorter MK,Conseil G,Deeley RG,Campbell RL,Cole SP

更新日期：2008-01-04 00:00:00

abstract：:The glucose-dependent insulinotropic peptide receptor (GIP-R) is a member of the secretin and parathyroid hormone (PTH) family of seven transmembrane-spanning receptors. Point mutations of a histidine at the junction between the first intracellular loop and the second membrane-spanning domain and a threonine in the si...

journal_title：Biochemical and biophysical research communications

authors： Tseng CC,Lin L

更新日期：1997-03-06 00:00:00

abstract：:CDX2 is an intestine-specific tumor suppressor gene encoding homeodomain-containing transcription factor, which is involved in a variety of developmental, proliferating, and differentiating processes. Moreover, the expression of CDX2 is reduced in a subset of primary colorectal cancers. In contrast, cyclooxygenase-2 (...

journal_title：Biochemical and biophysical research communications

authors： Kim SP,Park JW,Lee SH,Lim JH,Jang BC,Lee SH,Jang IH,Freund JN,Suh SI,Mun KC,Song DK,Ha EM,Lee WJ,Kwon TK

更新日期：2004-02-27 00:00:00

abstract：:Human lung carcinoma cells (A549) were oxidatively stressed with mildly-toxic or non-toxic amounts of hydrogen peroxide (H2O2, 0.1 mM to 120 mM) for 5 min. Hydrogen peroxide exposure resulted in a dose dependent inhibition of binding (pH 7) of the thiol reagent iodoacetic acid (IAA) to a 38 kDa cell protein. Incubatio...

journal_title：Biochemical and biophysical research communications

authors： Brodie AE,Reed DJ

更新日期：1987-10-14 00:00:00

abstract：:Tissue-specific alternative RNA splicing in human F1gamma pre-mRNA produces muscle- and nonmuscle-type isoforms. Muscle-specific exclusion of exon 9 of the F1gamma gene is cell-specifically induced by acidic treatment of human fibrosarcoma HT1080 and rhabdomyosarcoma KYM-1 cells. We constructed an F1gamma minigene con...

journal_title：Biochemical and biophysical research communications

authors： Hayakawa M,Endo H,Hamamoto T,Kagawa Y

更新日期：1998-10-20 00:00:00

abstract：:Licochalcone (LA) is a novel estrogenic flavonoid isolated from PC-SPES composition herb licorice root that was reported to show significant antitumor activity in various malignant human cell lines. To better understand its anti-CaP activities, we have investigated LA-elicited growth control and induction of apoptosis...

journal_title：Biochemical and biophysical research communications

authors： Fu Y,Hsieh TC,Guo J,Kunicki J,Lee MY,Darzynkiewicz Z,Wu JM

更新日期：2004-09-10 00:00:00

abstract：:A critical aspect of sexual development in Dictyostelium is the selection of "self" as a targeted food source by the phagocytic zygotic giant cell (ZGC). It has previously been demonstrated that phagocytic giant cells preferentially take up amoebae of the same species suggesting that the process is mediated by a speci...

journal_title：Biochemical and biophysical research communications

authors： Lewis KE,Jamieson JC

更新日期：1997-01-23 00:00:00

abstract：:It has been shown that the protein serine/threonine phosphatase type 2A alpha catalytic subunit (PP2A alpha) has a mutation consisting of a glycine substitution for cysteine at 269 in the okadaic acid resistant variant of CHO cells, and that the mutant protein is resistant to okadaic acid (Shima, H. et al. Proc. Natl....

journal_title：Biochemical and biophysical research communications

authors： Kaneko S,Shima H,Amagasa T,Takagi M,Sugimura T,Nagao M

更新日期：1995-09-14 00:00:00

abstract：:Enzyme activity in rat serum was examined utilizing the potent tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) and various glycerolipids as substrates. The serum activity was specific for hydrolysis of the long chain tetradecanoate moiety of TPA, hydrolyzed mono- and diacylglycerols, but was not effective ag...

journal_title：Biochemical and biophysical research communications

authors： Cabot MC

更新日期：1984-08-30 00:00:00

abstract：:In several inflammatory conditions such as rheumatoid arthritis or sepsis, the regulatory mechanisms of inflammation are inefficient and the excessive inflammatory response leads to damage to the host. Sirtuins are class III histone deacetylases that modulate the activity of several transcription factors that are impl...

journal_title：Biochemical and biophysical research communications

authors： Fernandes CA,Fievez L,Neyrinck AM,Delzenne NM,Bureau F,Vanbever R

更新日期：2012-04-20 00:00:00

abstract：:Trypsin-like serine proteases are involved in diverse biological processes such as complement activation, tissue remodeling, cellular migration, tumor invasion, and metastasis. Here we report a novel human C1r-like serine protease analog, CLSPa, derived from dendritic cells (DC). The 487-residue CLSPa protein contains...

journal_title：Biochemical and biophysical research communications

authors： Lin N,Liu S,Li N,Wu P,An H,Yu Y,Wan T,Cao X

更新日期：2004-08-20 00:00:00

abstract：:The green color of the hemolymph of last instar larvae of the corn earworm, Heliothis zea (Boddie), is caused by the appearance of a blue chromoprotein. This protein (Mr approximately 560,000) is composed of 4 subunits (Mr approximately 150,000). It contains 8.4% lipid and has an equilibrium density of 1.26 g/ml. This...

journal_title：Biochemical and biophysical research communications

authors： Haunerland NH,Bowers WS

更新日期：1986-01-29 00:00:00

abstract：:Acute stress has been shown to enhance learning and memory ability, predominantly through the action of corticosteroid stress hormones. However, the valuable targets for promoting learning and memory induced by acute stress and the underlying molecular mechanisms remain unclear. Acid-sensing ion channels (ASICs) play ...

journal_title：Biochemical and biophysical research communications

authors： Ye S,Yang R,Xiong Q,Yang Y,Zhou L,Gong Y,Li C,Ding Z,Ye G,Xiong Z

更新日期：2018-04-15 00:00:00

abstract：:Whether mitochondrial Ca(2+) transport is rapid enough to respond to changes in cytosolic [Ca(2+)] ([Ca(2+)](c)) which occur during excitation-contraction coupling in the heart is controversial; different results wereobtained with different techniques and different species. In this study mitochondrial [Ca(2+)] ([Ca(2+...

journal_title：Biochemical and biophysical research communications

authors： Griffiths EJ

更新日期：1999-09-24 00:00:00

abstract：:Two synthetic genes coding for human and Arabidopsis cytochrome c, respectively, have been designed and constructed, and the recombinant proteins have been over-expressed in Escherichia coli cells. Thus a comparative analysis of the two heme proteins, including horse cytochrome c as a reference, has been performed. In...

journal_title：Biochemical and biophysical research communications

authors： Rodríguez-Roldán V,García-Heredia JM,Navarro JA,Hervás M,De la Cerda B,Molina-Heredia FP,De la Rosa MA

更新日期：2006-08-04 00:00:00

abstract：:The vast majority of physiological processes in living cells are mediated by protein-protein interactions often specified by particular protein sequence motifs. PDZ domains, composed of 80-100 amino acid residues, are an important class of interaction motif. Among the PDZ-containing proteins, glutaminase interacting p...

journal_title：Biochemical and biophysical research communications

authors： Zencir S,Ovee M,Dobson MJ,Banerjee M,Topcu Z,Mohanty S

更新日期：2011-08-12 00:00:00

abstract：:Here we enhanced the stability and biophysical properties of mRFP1 through a combination of canonical and non-canonical amino acid mutagenesis. The global replacement of proline residue with (2S, 4R)-4-fluoroproline [(4R)-FP] into mRFP1 led to soluble protein but lost its fluorescence, whereas (2S, 4S)-4-fluoroproline...

journal_title：Biochemical and biophysical research communications

authors： Deepankumar K,Nadarajan SP,Ayyadurai N,Yun H

更新日期：2013-11-01 00:00:00

abstract：:Recent studies suggest important roles for long noncoding RNAs as essential regulators of human cancer. GClnc1 was found to be upregulated in gastric cancer, playing oncogenic roles. However, the biological function and underlying mechanism of GClnc1 in osteosarcoma (OS) remain unclear. Here, we report that GClnc1 is ...

journal_title：Biochemical and biophysical research communications

authors： Sui Y,Han Y,Zhao X,Li D,Li G

更新日期：2018-12-09 00:00:00