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Isolation and expression of a guanylate cyclase-coupled heat stable enterotoxin receptor cDNA from a human colonic cell line.

Abstract：

:Heat stable enterotoxins (STs) are low molecular-weight peptides secreted by enterotoxigenic bacteria. One type of these enterotoxins (STa) induces intestinal secretion leading to acute diarrhea by binding to a membrane form of guanylate cyclase. We have isolated a cDNA from a human colonic cell line, T84, encoding for a guanylate cyclase-coupled enterotoxin receptor (STaR). The predicted amino acid sequence of the human STa receptor is 81% identical with the previously cloned enterotoxin receptor (GC-C) from rat intestine. COS-7 cells transiently transfected with the cloned cDNA expressed specific concentration-dependent response to STa as measured by cyclic GMP accumulation and is about 20 times more sensitive to the stimulation by STa than has been shown for GC-C.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Singh S,Singh G,Heim JM,Gerzer R

doi

10.1016/0006-291x(91)91736-v

subject

Has Abstract

pub_date

1991-09-30 00:00:00

pages

1455-63

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91736-V

journal_volume

179

pub_type

杂志文章

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