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Augmentation of antigen-specific immune responses using DNA-fusogenic liposome vaccine.

Abstract：

:In an attempt to enhance the immunological efficacy of genetic immunization, we investigated a new biological means for delivering antigen gene directly to the cytoplasm via membrane fusion. In this context, we investigated fusogenic liposome (FL) encapsulating DNA as a possible genetic immunization vehicle. RT-PCR analysis indicated that a FL could introduce and express encapsulating OVA gene efficiently and rapidly in vitro. Consistent with this observation, an in vitro assay showed that FL-mediated antigen-gene delivery can induce potent presentation of antigen via the MHC class I-dependent pathway. Accordingly, immunization with FL containing the OVA-gene induced potent OVA-specific Th1 and Th2 cytokine production. Additionally, OVA-specific CTL responses and antibody production were also observed in systemic compartments including the spleen, upon immunization with the OVA-gene encapsulating FL. These findings suggest that FL is an effective genetic immunization carrier system for the stimulation of antigen-specific immune responses against its encoding antigen.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Yoshikawa T,Imazu S,Gao JQ,Hayashi K,Tsuda Y,Shimokawa M,Sugita T,Niwa T,Oda A,Akashi M,Tsutsumi Y,Mayumi T,Nakagawa S

doi

10.1016/j.bbrc.2004.10.056

subject

Has Abstract

pub_date

2004-12-10 00:00:00

pages

500-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(04)02378-2

journal_volume

325

pub_type

杂志文章

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