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Purification and partial characterization of two cyclic amp-independent protein kinases from AH-66 hepatoma cells.

Abstract：

:Two cyclic AMP-independent protein kinases (CK 1 and CK 2) in the cytosol fraction of AH-66 hepatoma cells have been purified to homogeneity. CK 1 and CK 2 had molecular weights of 36,000 and 340,000, respectively, as determined by gel filtration. CK 1 consists of a single polypeptide and CK 2 is composed of two subunits of 42,000 and 26,000 molecular weights. CK 1 and the 26,000 molecular weight subunit of CK 2 were autophosphorylated. Both enzymes catalyzed the phosphorylation of cytosolic proteins including those with molecular weights of 125,000, 95,000, 56,000, and 40,000.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Nakajo S,Shimizu T,Shinkawa K,Nakaya K,Nakamura Y

doi

10.1016/0006-291x(83)91099-9

subject

Has Abstract

pub_date

1983-06-29 00:00:00

pages

1010-7

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(83)91099-9

journal_volume

113

pub_type

杂志文章

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