Is cAMP the obligatory second messenger in the action of lutropin on Leydig cell steroidogenesis.

abstract：:Interferon gamma (IFN-gamma) plays an important role in immune response, apoptosis, and anti-tumor activity. Its biological activity depends on expression of IFN-gamma receptor (IFN-gammaR). To address whether increased expression of IFN-gammaR is associated in vivo with a higher biological response by IFN-gamma, we c...

journal_title：Biochemical and biophysical research communications

authors： Nishida Y,Maeda Y,Hara A,Arima T,Kimura E,Yamashita S,Uyama E,Mita S,Uchino M

更新日期：2002-01-25 00:00:00

abstract：:Fluorescein 5'-isothiocyanate (FITC) was used to modify lysine residues of the strychnine-sensitive glycine receptor. Pretreatment of rat spinal cord synaptic plasma membranes with FITC specifically affected the ability of glycine to displace [3H]strychnine binding. Glycine completely prevented the effect of FITC modi...

journal_title：Biochemical and biophysical research communications

authors： Ruiz Gómez A,Fernández-Shaw C,Valdivieso F,Mayor F Jr

更新日期：1989-04-14 00:00:00

abstract：:Recent studies have demonstrated that adult cells such as pancreatic exocrine cells can be converted to pancreatic β-cells in a process called cell reprogramming. Enteroendocrine cells and β-cells share similar pathways of differentiation during embryonic development. Notably, enteroendocrine K cells express many of t...

journal_title：Biochemical and biophysical research communications

authors： Lee E,Ryu GR,Moon SD,Ko SH,Ahn YB,Song KH

更新日期：2014-01-17 00:00:00

abstract：:Peptidylarginine deiminases (PADs) convert arginine residues in proteins into citrulline residues Ca(2+)-dependently. PAD V was recently found in granulocyte-differentiated HL-60 cells. To find a target of PAD V, we incubated HL-60 granulocytes with the calcium ionophore A23187 and studied deiminated proteins by immun...

journal_title：Biochemical and biophysical research communications

authors： Hagiwara T,Nakashima K,Hirano H,Senshu T,Yamada M

更新日期：2002-01-25 00:00:00

abstract：:In this study, we use HRMAS NMR as a non-invasive technique to monitor the in vivo metabolism of a xenobiotic. The antituberculosis Ethionamide is a pro-drug that has to be activated in mycobacteria before inhibiting its cellular target. The use of (1)H HRMAS NMR has allowed to detect a metabolite (ETH*) of the drug d...

journal_title：Biochemical and biophysical research communications

authors： Hanoulle X,Wieruszeski JM,Rousselot-Pailley P,Landrieu I,Baulard AR,Lippens G

更新日期：2005-06-03 00:00:00

abstract：:Endothelin-1 (ET-1) contracted the rabbit tracheal smooth muscle (RTSM), yielding a bell-shaped tension-concentration curve. Moreover, ET-1 induced concentration- and time-dependent increases in cAMP concentrations in RTSM (EC(50), 58 nM; t(1/2), 2.4 min). Pretreatment with the AC inhibitors, SQ-22536, or 2'-5'-dideox...

journal_title：Biochemical and biophysical research communications

authors： El-Mowafy AM,Biggs DF

更新日期：2001-08-17 00:00:00

abstract：:Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted ...

journal_title：Biochemical and biophysical research communications

authors： Korc M,Matrisian LM,Planck SR,Magun BE

更新日期：1983-03-29 00:00:00

abstract：:Tocopherols (vitamin E) located in the hydrophobic domains of biological membranes act as chain breaking antioxidants preventing the propagation of free radical reactions of lipid peroxidation. The naturally occurring form, d-alpha tocopherol is an exquisite molecule in that it is intercalated in the membrane in such ...

journal_title：Biochemical and biophysical research communications

authors： Stoyanovsky DA,Kagan VE,Packer L

更新日期：1989-04-28 00:00:00

abstract：:By performing immunofluorescence staining assays in confluent COS-7 cells, hepatitis B virus core, small surface and large surface proteins were found to localize largely in the nucleus, diffusely in the cytoplasm, and accumulatively in a perinuclear area, respectively. When two proteins were co-expressed, the core pr...

journal_title：Biochemical and biophysical research communications

authors： Yeh CT,Ou JH,Chu CM,Liaw YF

更新日期：1994-09-15 00:00:00

abstract：:Endostatin, a 20 kDa C-terminal fragment of collagen XVIII, is a specific inhibitor of endothelial cell proliferation and angiogenesis. In the present study, we have expressed human endostatin in a yeast expression system (10 mg/L). The recombinant protein was expressed in a soluble form and purified to homogeneity. I...

journal_title：Biochemical and biophysical research communications

authors： Dhanabal M,Volk R,Ramchandran R,Simons M,Sukhatme VP

更新日期：1999-05-10 00:00:00

abstract：:The recent discovery that mutation Asn21 --> Ile in the human cationic trypsinogen (Tg) is associated with hereditary pancreatitis has brought into focus the functional role of amino acid 21 in mammalian Tgs. In the present paper, the effect of mutations Thr21 --> Asn and Thr21 --> Ile on the Ca(2+) dependence of zymo...

journal_title：Biochemical and biophysical research communications

authors： Sahin-Tóth M,Tóth M

更新日期：2000-08-28 00:00:00

abstract：:Induction of glycogenolysis in the perfused liver by platelet activating factor (PAF) was blocked by the cyclooxygenase inhibitor indomethacin. 3H-labeled PAF was shown to interact in the perfused liver primarily with Kupffer cells. The addition of PAF to Kupffer cells resulted in a dose-dependent stimulation of prost...

journal_title：Biochemical and biophysical research communications

authors： Kuiper J,De Rijke YB,Zijlstra FJ,Van Waas MP,Van Berkel TJ

更新日期：1988-12-30 00:00:00

abstract：:Studies were undertaken to determine the immunochemical relationship between constitutive trout cytochrome P450s and mammalian cytochrome P450IIIA enzymes. Polyclonal antibodies (IgG) generated against trout P450 LMC5 reacted strongly with P450IIIA1 in dexamethasone-induced rat liver microsomes and with P450IIIA4 in h...

journal_title：Biochemical and biophysical research communications

authors： Miranda CL,Wang JL,Henderson MC,Zhao X,Guengerich FP,Buhler DR

更新日期：1991-04-30 00:00:00

abstract：:The non-receptor tyrosine kinases c-Src and focal adhesion kinase (Fak) mediate signal transduction pathways that regulate cell proliferation, survival, invasion, and metastasis. Here, we investigated whether c-Src and Fak are activated during progression of hormone-dependent breast cancer. Maximally active c-Src was ...

journal_title：Biochemical and biophysical research communications

authors： Planas-Silva MD,Bruggeman RD,Grenko RT,Stanley Smith J

更新日期：2006-03-03 00:00:00

abstract：:The smallest protein of hepatitis B virus, HBX, has been implicated in the development of liver diseases by interfering with normal cellular processes. Its role in cell proliferation has been unclear as both pro-apoptotic and anti-apoptotic activities have been reported. We showed molecular evidence that HBX induced a...

journal_title：Biochemical and biophysical research communications

authors： Lu YW,Chen WN

更新日期：2005-12-23 00:00:00

abstract：:Growth and differentiation factors (GDF)-5, -6, and -7 are members of the bone morphogenetic protein (BMP) family. Previous studies suggest their importance in bone development and in tendon/ligament morphogenesis. The cells of the dental attachment apparatus, cementum, periodontal ligament, and alveolar bone proper a...

journal_title：Biochemical and biophysical research communications

authors： Morotome Y,Goseki-Sone M,Ishikawa I,Oida S

更新日期：1998-03-06 00:00:00

abstract：:Intracellular transport and storage of retinoids were shown to be conducted by fatty acid-binding protein (FABP). When rat liver cytosol was gel filtrated, retinyl palmitate-binding activity was mainly eluted in the fraction with a Mr. of around 14,000, in which both FABP and cellular retinol-binding protein (CRBP) co...

journal_title：Biochemical and biophysical research communications

authors： Fukai F,Kase T,Shidotani T,Nagai T,Katayama T

更新日期：1987-09-30 00:00:00

abstract：:Ap4A hydrolase (asymmetrical diadenosine tetraphosphate hydrolase, EC 3.6.1.17), an enzyme involved in a number of biological processes, is characterized as cleaving the polyphosphate chain at the fourth phosphate from the bound adenosine moiety. This paper presents the crystal structure of wild-type and E58A mutant h...

journal_title：Biochemical and biophysical research communications

authors： Ge H,Chen X,Yang W,Niu L,Teng M

更新日期：2013-03-01 00:00:00

abstract：:Actin-related proteins share significant homology with conventional actins and are classified into subfamilies based on the similarity of their sequences and functions. The Arp4 subfamily of Arps is localized in the nucleus, and a mammalian isoform, ArpNbeta (also known as BAF53), is a component of the chromatin remod...

journal_title：Biochemical and biophysical research communications

authors： Kuroda Y,Oma Y,Nishimori K,Ohta T,Harata M

更新日期：2002-11-29 00:00:00

abstract：:Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhib...

journal_title：Biochemical and biophysical research communications

authors： Bandyopadhyay U,Kaiser T,Rudolf MT,Schultz C,Guse AH,Mayr GW

更新日期：1997-11-07 00:00:00

abstract：:Three metallo forms of peptide deformylase (PDF, EC 3.5.1.31) of Escherichia coli were prepared and crystallized (space group C2, diffraction limit 1.9 A) for initiating the X-ray structure determination of the metal center in correlation with the catalytic functionality of this enzyme. The native Fe2+ containing enzy...

journal_title：Biochemical and biophysical research communications

authors： Groche D,Becker A,Schlichting I,Kabsch W,Schultz S,Wagner AF

更新日期：1998-05-19 00:00:00

abstract：:The recent release of COVID-19 spike glycoprotein allows detailed analysis of the structural features that are required for stabilizing the infective form of its quaternary assembly. Trying to disassemble the trimeric structure of COVID-19 spike glycoprotein, we analyzed single protomer surfaces searching for concave ...

journal_title：Biochemical and biophysical research communications

authors： Bongini P,Trezza A,Bianchini M,Spiga O,Niccolai N

更新日期：2020-07-12 00:00:00

abstract：:The microtubule motor kinesin-1 is responsible for the nuclear positioning during myogenesis. Here we show that the coiled-coil stalk/tail domain containing the kinesin light chain (KLC) binding sites targets to the perinuclear region like endogenous Kif5b, while the globular tail domain cannot. To investigate which f...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Xue W,Li X,Lin R,Cui J,Huang JD

更新日期：2013-03-08 00:00:00

abstract：:By the use of well-characterized antibodies against porcine dynorphin-A(1-8), an endogenous opioid peptide, and the use of a modified immunofluorescence microscopic technique, dynorphin-A(1-8) stained perikarya, nerve fibres, and nerve terminals were visualized in the rat duodenum. Dynorphin-A(1-8) immunoreactive peri...

journal_title：Biochemical and biophysical research communications

authors： Wolter HJ

更新日期：1985-03-15 00:00:00

abstract：:A radioactive photoaffinity probe for the insulin receptor was prepared by derivatizing insulin at its B29 lysine with a novel crosslinking reagent having a cleavable azo linkage. Insulin receptors purified from human placental membranes were photoaffinity labeled with this probe. The photolabeled receptor was treated...

journal_title：Biochemical and biophysical research communications

authors： Ng DS,Yip CC

更新日期：1985-11-27 00:00:00

abstract：:Glial cells have been shown to induce enzyme activities, which are characteristically high in brain capillaries, in cerebral and non cerebral endothelial cells in culture. We evaluated the activity of gamma-glutamyl transpeptidase (gamma GT) in freshly isolated (ex vivo) bovine blood (BEC) and lymphatic (LEC) endothel...

journal_title：Biochemical and biophysical research communications

authors： Weber E,Lorenzoni P,Raffaelli N,Cavina N,Sacchi G

更新日期：1996-08-23 00:00:00

abstract：:In AL-amyloidosis the cause of amyloid fibril formation in beta-pleated sheets from the precursor protein immunoglobulin light chain is not established, but studies of AL-proteins indicate that amino acid substitutions are important in the pathogenesis. Amyloid material was extracted from a subcutaneous fat tissue bio...

journal_title：Biochemical and biophysical research communications

authors： Olsen KE,Sletten K,Westermark P

更新日期：1998-04-28 00:00:00

abstract：:A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihydroxy-ethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)2S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polyme...

journal_title：Biochemical and biophysical research communications

authors： Burow M,Minoura N

更新日期：1996-10-14 00:00:00

abstract：:Neuropathic pain is triggered by damage to or as a result of the dysfunction of the somatosensory nervous system. Gene expression profiling using DNA microarray and real-time PCR have emerged as powerful tools for the elucidation of pain-specific pathways and identification of candidate biomarkers and therapeutic targ...

journal_title：Biochemical and biophysical research communications

authors： Wan G,Yang K,Lim Q',Zhou L,He BP,Wong HK,Too HP

更新日期：2010-10-01 00:00:00

abstract：:The role of microRNAs (miRNAs) in how microbiota influence the host intestinal immune system is not fully understood. We compared the expression profiles of miRNAs and mRNAs in lamina propria leukocytes (LPL) in the large intestines of germ-free (GF) and specific pathogen-free (SPF) mice. Microarray analysis revealed ...

journal_title：Biochemical and biophysical research communications

authors： Ohsaka F,Karatsu Y,Kadota Y,Tochio T,Takemura N,Sonoyama K

更新日期：2021-01-01 00:00:00