Molecular imprinting: synthesis of polymer particles with antibody-like binding characteristics for glucose oxidase.

Abstract:

:A phosphate buffer containing a mixture of glucose oxidase, acrylic acid derivatives, N,N'-1,2 dihydroxy-ethylene-bis(acrylamide), N,N'-(methylene)-bisacrylamide and surface-modified silica was radically polymerized with (NH4)2S2O8. The polymer formed a thin layer around the silica beads. After sieving of these polymer particles, the surface bound protein was eluted. In rebinding assays and enzyme activity tests a specific binding capacity for glucose oxidase of up to 0.557 microgram GOD/100 mg dry weight of polymer particles could be determined. These polymer particles have the potential to be used as specific separation or detection material.

authors

Burow M,Minoura N

doi

10.1006/bbrc.1996.1522

subject

Has Abstract

pub_date

1996-10-14 00:00:00

pages

419-22

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)91522-3

journal_volume

227

pub_type

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