Abstract：
:The spectrum of resting cytochrome c oxidase is modulated by valinomycin addition, which induces a red shift of the Soret band. The enzyme is known both to fluoresce and phosphorescence, effects which can be modulated by certain protein reagents and quenchers. Valinomycin had little effect upon fluorescence at 335 nm, whether excited at 295 nm or 280 nm (tryptophans or both tryptophans and tyrosines, respectively). Phosphorescence at 445 nm was slightly enhanced upon the binding of valinomycin to the enzyme, suggesting a small conformational change accompanying the spectral shift of the heme groups. The quenching by nitrite of the phosphorescence excited at 260 nm, but not that excited at 295 nm, was diminished by valinomycin. This suggests that the valinomycin-induced conformational change may involve (i) a change in the accessibility of tyrosines to the quencher and/or (ii) a change in distribution of distances and/or orientations between populations of tyrosines and tryptophans.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Butko P,He J,Nicholls Pdoi
10.1016/0006-291x(92)90241-csubject
Has Abstractpub_date
1992-12-30 00:00:00pages
1477-83issue
3eissn
0006-291Xissn
1090-2104pii
0006-291X(92)90241-Cjournal_volume
189pub_type
杂志文章abstract：:Immortal hepatocytes were established from a CYP3A7-transgenic/p53-knockout mouse. The cells could be maintained with shorter doubling time after culture for 1 month. Detectable amounts of CYP3A7 mRNA and the activity of 7-propoxycoumarin O-depropylase, one of the representative CYP3A activities, were present in the i...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1995.2869
更新日期：1995-12-26 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2007-12-28 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.07.206
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.03.078
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1996.0189
更新日期：1996-02-06 00:00:00
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2007-12-07 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：1997-04-07 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2019.05.081
更新日期：2019-07-12 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2009-02-06 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1995.1037
更新日期：1995-01-05 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1999.0361
更新日期：1999-04-02 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1993.1925
更新日期：1993-08-16 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2019.03.196
更新日期：2019-05-28 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2000-07-14 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2016.05.157
更新日期：2016-08-05 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.2001.6298
更新日期：2002-01-25 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2017.07.134
更新日期：2017-09-30 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1997.8059
更新日期：1998-02-04 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2007.04.018
更新日期：2007-06-08 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1006/bbrc.1993.2577
更新日期：1993-12-15 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2005.03.071
更新日期：2005-05-13 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(84)90454-6
更新日期：1984-07-18 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2017.09.119
更新日期：2017-11-18 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2005.09.139
更新日期：2005-11-25 00:00:00
abstract：:Apoptosis and autophagy are crucial mechanisms regulating cell death, and the relationship between apoptosis and autophagy in the liver has yet to be thoroughly explored. TIGAR (TP53-induced glycolysis and apoptosis regulator), which is a p53-inducible gene, functions in the suppression of ROS (reactive oxygen species...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2013.06.072
更新日期：2013-07-26 00:00:00
abstract：:Cytochrome P450 enzymes (CYP enzymes) catalyse the initial step in biotransformation of xenobiotics like polycyclic aromatic hydrocarbons (PAHs). The marine polychaete Nereis virens has a high capacity for biotransformation of PAHs. In the present study, the complete cDNA sequences of two novel CYP genes isolated from...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2005.08.189
更新日期：2005-10-28 00:00:00
