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Shiga-like toxin-based high-efficiency and receptor-specific intracellular delivery system for a protein.

Abstract：

:The cell-specific cytosolic delivery of functional macromolecules with high efficiency is of great significance in molecular medicine and biotechnology. Herein, we present a Shiga-like toxin II-based high-efficiency and receptor-specific intracellular delivery system. We designed and constructed the Shiga-like toxin-based carrier (STC) to comprise the targeting and translocation domains, and used it for delivering a protein cargo. The STC was shown to deliver a protein cargo into the cytosol with high efficiency in a receptor-specific manner, exhibiting much higher efficiency than the most widely used cell-penetrating peptide. The general utility of the STC was demonstrated by modulating the targeting domain. The present delivery platform can be widely used for the intracellular delivery of diverse biomolecules in a receptor-specific and genetically encodable manner.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ryou JH,Sohn YK,Hwang DE,Kim HS

doi

10.1016/j.bbrc.2015.07.122

subject

Has Abstract

pub_date

2015-09-04 00:00:00

pages

1282-1289

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)30348-X

journal_volume

464

pub_type

杂志文章

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