Molecular cloning of cDNA for human poly(ADP-ribose) polymerase and expression of its gene during HL-60 cell differentiation.

abstract：:Manganese-dependent superoxide dismutase (MnSOD) is localized in the mitochondria and is important for oxidative stress resistance. Although transcriptional regulation of MnSOD has been relatively well studied, much less is known about the protein's posttranslational regulation. In budding yeast, MnSOD is activated af...

journal_title：Biochemical and biophysical research communications

authors： Takahashi H,Suzuki T,Shirai A,Matsuyama A,Dohmae N,Yoshida M

更新日期：2011-03-04 00:00:00

abstract：:Hypoxic/ischemic trauma is a primary factor in the pathology of various disease states. Yet, very little is known about the molecular mechanisms involved in cellular responses and adaptations to hypoxia. As a means of identifying intracellular signaling systems that are regulated in response to hypoxia, the effects of...

journal_title：Biochemical and biophysical research communications

authors： Beitner-Johnson D,Leibold J,Millhorn DE

更新日期：1998-01-06 00:00:00

abstract：:Human dendritic cells (DC) are key regulators of innate and adaptive immunity that can be divided in at least three major subpopulations: plasmacytoid DC (pDC), myeloid type 1 DC (mDC1) and myeloid type 2 DC (mDC2) exhibiting different functions. However, research, diagnostic and cell therapeutic studies on human DC s...

journal_title：Biochemical and biophysical research communications

authors： Schütz F,Hackstein H

更新日期：2014-01-10 00:00:00

abstract：:The stage V sporulation protein G (SpoVG) homolog of Staphylococcus aureus is a modulator of virulence factor synthesis and antibiotic resistance in this clinically important gram-positive pathogen. Here we demonstrate that SpoVG can be phosphorylated by the staphylococcal Ser/Thr protein kinase Stk1 and that phosphor...

journal_title：Biochemical and biophysical research communications

authors： Bischoff M,Brelle S,Minatelli S,Molle V

更新日期：2016-05-13 00:00:00

abstract：:We have recently reported that mutant but not wild-type (wt) p53 protein was ADP-ribosylated in primary rat cells overexpressing the temperature-sensitive murine p53val135 gene. To examine whether the lack of susceptibility to modification is a specific feature of p53val135 adopting wt conformation or rather a general...

journal_title：Biochemical and biophysical research communications

authors： Wesierska-Gadek J,Schmid G,Cerni C

更新日期：1996-07-05 00:00:00

abstract：:Cellular defects which prevent apoptotic cell death can result in the generation of hyperproliferative disorders and can prevent the effective treatment of such diseases. The majority of cellular defects which result in apoptosis resistance lie upstream of caspase activation. We have described chimeric caspase molecul...

journal_title：Biochemical and biophysical research communications

authors： Shearwin-Whyatt L,Baliga B,Doumanis J,Kumar S

更新日期：2001-04-20 00:00:00

abstract：:Arginine kinase catalyzes the reversible transfer of a phosphoryl group between ATP and l-arginine and is a monomeric homolog of the human enzyme creatine kinase. Arginine and creatine kinases belongs to the phosphagen kinase family of enzymes, which consists of eight known members, each of which is specific for its o...

journal_title：Biochemical and biophysical research communications

authors： Clark SA,Davulcu O,Chapman MS

更新日期：2012-10-12 00:00:00

abstract：:The Ca2+- and phospholipid-dependent protein kinase C from rat brain phosphorylates rabbit muscle phosphofructokinase at the same trypsin-labile site as cyclic AMP-dependent protein kinase. However, protein kinase C also effectively phosphorylates one or more separate sites. Incubation of phosphofructokinase in the pr...

journal_title：Biochemical and biophysical research communications

authors： Hofer HW,Schlatter S,Graefe M

更新日期：1985-06-28 00:00:00

abstract：:Atrial natriuretic factor (ANF) rapidly enhanced phosphorylation of plasma membrane proteins of bovine adrenal cortical cells. Pretreatment of the membranes with ANF (1 x 10(-8)M to 1 x 10(-7)M) resulted two- to four-fold in an incorporation of 32p-radioactivity from [gamma -32p]ATP as compared to the controls. The gu...

journal_title：Biochemical and biophysical research communications

authors： Pandey KN

更新日期：1989-09-15 00:00:00

abstract：:Incubation of lipoxin A4 (LXA4) either with human leukocytes or with rat liver microsomes in the presence of NADPH very selectively led to a more polar metabolite retaining the conjugated tetraenic structure of LXA4. Lipoxin B4 (LXB4) underwent a very similar metabolism into a more polar metabolite, whereas the all-tr...

journal_title：Biochemical and biophysical research communications

authors： Boucher JL,Delaforge M,Mansuy D

更新日期：1991-05-31 00:00:00

abstract：:Quantitative real-time RT-PCR was used to investigate the effects of prototypical drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on mRNA expression levels of the housekeeping genes beta-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-glucuronidase (GU...

journal_title：Biochemical and biophysical research communications

authors： Nishimura M,Koeda A,Suzuki E,Shimizu T,Kawano Y,Nakayama M,Satoh T,Narimatsu S,Naito S

更新日期：2006-08-04 00:00:00

abstract：:The sulfonation of estrogens by human estrogen sulfotransferase (humSULT1E1) plays a vital role in controlling the active levels of these hormones in the body. To understand more fully the structural and functional characteristics of humSULT1E1, we have carried out site-directed mutagenesis of critical amino acids fou...

journal_title：Biochemical and biophysical research communications

authors： Hempel N,Barnett AC,Bolton-Grob RM,Liyou NE,McManus ME

更新日期：2000-09-16 00:00:00

abstract：:The interdependency of protein and RNA syntheses was studied comparatively in bacteria confronted with amino acid starvation or treated separately with various aminoglycoside antibiotics. By contrast with the concomitant inhibition of macromolecular syntheses in cells deprived of an essential amino acid, RNA productio...

journal_title：Biochemical and biophysical research communications

authors： Cortay JC,Cozzone AJ

更新日期：1983-05-16 00:00:00

abstract：:Neural stem cells (NSCs) have the potential for differentiation into neurons known as a groundbreaking therapeutic solution for central nervous system (CNS) diseases. To resolve the therapeutic efficiency of NSCs, recent researchers have focused on the study on microRNA's role in CNS. Some micro RNAs have been reporte...

journal_title：Biochemical and biophysical research communications

authors： Song J,Cho KJ,Oh Y,Lee JE

更新日期：2015-07-10 00:00:00

abstract：:Stefin B is the major general cytosolic protein inhibitor of cysteine cathepsins. Its main function is to protect the organism against the activity of endogenous potentially hazardous proteases accidentally released from lysosomes. In this study, we investigated the possible effect of endosomal/lysosomal aspartic cath...

journal_title：Biochemical and biophysical research communications

authors： Železnik TZ,Kadin A,Turk V,Dolenc I

更新日期：2015-09-18 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, Ca(2+) signaling mediated by the Ca(2+)/calmodulin dependent phosphatase, calcineurin, is required for survival during environmental stress. One role of the phosphatase under these conditions is to activate gene expression through its regulation of the Crz1p ("crazy") transcripti...

journal_title：Biochemical and biophysical research communications

authors： Cyert MS

更新日期：2003-11-28 00:00:00

abstract：:Botulinum neurotoxins (BoNTs) are produced as progenitor toxin complexes (PTCs) by Clostridium botulinum. The PTCs are composed of BoNT and non-toxic neurotoxin-associated proteins (NAPs), which serve to protect and deliver BoNT through the gastrointestinal tract in food borne botulism. HA33 is a key NAP component tha...

journal_title：Biochemical and biophysical research communications

authors： Lee K,Lam KH,Kruel AM,Perry K,Rummel A,Jin R

更新日期：2014-04-04 00:00:00

abstract：:The mouse disabled 2 interacting protein 2 (mDaIP2) had been obtained through yeast two hybrid system. It consists of 506 amino acids and its calculated molecular weight is 57.7 kDa. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain interacts with the proline rich domain of mDab2 whi...

journal_title：Biochemical and biophysical research communications

authors： Choi YJ,Cho SY,Kim HW,Kim JA,Bae SH,Park SS

更新日期：2005-01-21 00:00:00

abstract：:Farnesoid X receptor (FXR) belongs to the ligand-activated nuclear receptor superfamily, and functions as a transcription factor regulating the transcription of numerous genes involved in bile acid homeostasis, lipoprotein and glucose metabolism. In the present study, we identified RECK, a membrane-anchored inhibitor ...

journal_title：Biochemical and biophysical research communications

authors： Peng X,Wu W,Zhu B,Sun Z,Ji L,Ruan Y,Zhou M,Zhou L,Gu J

更新日期：2014-01-03 00:00:00

abstract：:The control of cellular adhesion to extracellular matrix proteins is poorly understood. In the present analyses, we set out to test the hypothesis that high galectin-3 concentration on the cell surface downregulates cellular adhesion to the extracellular matrix proteins. Various tumor cell lines were briefly incubated...

journal_title：Biochemical and biophysical research communications

authors： Ochieng J,Leite-Browning ML,Warfield P

更新日期：1998-05-29 00:00:00

abstract：:Dysfunction of Girdin plays a crucial role in the development of a variety of tumors. Phosphorylated regulation of Girdin has been studied extensively. However, how Girdin is dephosphorylated remains unclear. In this study, we report a mechanism of Girdin dephosphorylation and the importance of this mechanism in the m...

journal_title：Biochemical and biophysical research communications

authors： Li J,Enomoto A,Weng L,Sun L,Takahashi M

更新日期：2019-05-21 00:00:00

abstract：:Using a highly purified preparation of glycine methyltransferase mRNA, double-stranded cDNA was synthesized and inserted into the PstI site of pBR322. The resulting recombinant DNA was used to transform E. coli X 1776 by conventional methods. Among tetracycline-resistant transformants, a number of colonies were found ...

journal_title：Biochemical and biophysical research communications

authors： Ogawa H,Gomi T,Horii T,Ogawa H,Fujioka M

更新日期：1984-10-15 00:00:00

abstract：:Strong temperature dependent low angle X-ray scattering by trifluoroethanol- and hexafluoro-2-propanol-water mixtures was observed in conditions commonly used in NMR work on peptides. At least two types of molecular effects may contribute to the observed scattering: formation of clathrate hydrate-like aggregates of al...

journal_title：Biochemical and biophysical research communications

authors： Kuprin S,Gräslund A,Ehrenberg A,Koch MH

更新日期：1995-12-26 00:00:00

abstract：:A sulfotransferase activity present in the cytosol fraction of rat submandibular and parotid salivary glands catalyzes the transfer of sulfate group from 3'-phosphoadenosine-5'-phosphosulfate to C-6 of the terminal glucose residue of Glc alpha 1 leads to 6Glc alpha 1 leads to 6Glc alpha 1 leads to 3-1, (3)-0-alkyl-2-0...

journal_title：Biochemical and biophysical research communications

authors： Slomiany BL,Liau YH,Zdebska E,Murty VL,Slomiany A

更新日期：1983-06-29 00:00:00

abstract：:Novel erythrocyte pyruvate kinase gene defects were found in a patient without a family history of consanguinity. The polymerase chain reaction products of the R-type pyruvate kinase cDNA from the propositus contained two point mutations of Ser80 (TCC)-->Pro (CCC) and Arg490 (CGG)-->Trp (TGG). Allele-specific polymera...

journal_title：Biochemical and biophysical research communications

authors： Uenaka R,Nakajima H,Noguchi T,Imamura K,Hamaguchi T,Tomita K,Yamada K,Kuwajima M,Kono N,Tanaka T

更新日期：1995-03-28 00:00:00

abstract：:Protein kinase D (PKD) has been established as a negative modulator of the c-Jun N-terminal kinase (JNK) signaling pathway. We previously demonstrated that induced expression of constitutively active PKD (PKD-S744/748E) that mimics phosphorylation by PKC is sufficient to attenuate epidermal growth factor (EGF) stimula...

journal_title：Biochemical and biophysical research communications

authors： Hurd C,Rozengurt E

更新日期：2003-03-21 00:00:00

abstract：:Hepatocyte Growth Factor (HGF) was originally identified as hepatotrophic factor for liver regeneration. More recently, HGF was revealed to stimulate proliferation of various epithelial cells. At present study, we have investigated the mitogenic effect of HGF on gastric epithelial cells in the primary culture system. ...

journal_title：Biochemical and biophysical research communications

authors： Takahashi M,Ota S,Terano A,Yoshiura K,Matsumura M,Niwa Y,Kawabe T,Nakamura T,Omata M

更新日期：1993-03-15 00:00:00

abstract：:To isolate seizure-related genes, we applied differential screening technique to the cDNA library which constructed from primary cultured cerebral cortical cells treated with pentylenetetrazol (PTZ). Northern blotting analysis of mRNA levels in the cerebra after systemic administration of PTZ confirmed the results of ...

journal_title：Biochemical and biophysical research communications

authors： Kajiwara K,Nagawawa H,Shimizu-Nishikawa S,Ookuri T,Kimura M,Sugaya E

更新日期：1996-02-27 00:00:00

abstract：:Myogenesis is regulated by the basic helix-loop-helix (bHLH) myogenic regulatory factor MyoD, which induces muscle-specific gene expression by binding to the E-box sequence as a heterodimer with ubiquitous bHLH E2A (E12/E47) proteins. Here, we report that a 31-kDa caspase-generated cleavage product of Crk-associated s...

journal_title：Biochemical and biophysical research communications

authors： Jeong DE,Lee EK,Song WK,Kim W

更新日期：2014-02-21 00:00:00

abstract：:Autoimmune sera showing prominent immunofluorescence in nucleolus were selected and analysed by immunoblotting techniques. Immunoblots using a nucleolar extract as antigen source revealed sera recognizing a 38 kDa nucleolar protein. Low concentration of Actinomycin D, which inhibits the ribosomal RNA synthesis, caused...

journal_title：Biochemical and biophysical research communications

authors： Kindås-Mügge I

更新日期：1989-09-15 00:00:00