Let7a involves in neural stem cell differentiation relating with TLX level.

Abstract:

:Neural stem cells (NSCs) have the potential for differentiation into neurons known as a groundbreaking therapeutic solution for central nervous system (CNS) diseases. To resolve the therapeutic efficiency of NSCs, recent researchers have focused on the study on microRNA's role in CNS. Some micro RNAs have been reported significant functions in NSC self-renewal and differentiation through the post-transcriptional regulation of neurogenesis genes. MicroRNA-Let7a (Let7a) has known as the regulator of diverse cellular mechanisms including cell differentiation and proliferation. In present study, we investigated whether Let7a regulates NSC differentiation by targeting the nuclear receptor TLX, which is an essential regulator of NSC self-renewal, proliferation and differentiation. We performed the following experiments: western blot analysis, TaqMan assay, RT-PCR, and immunocytochemistry to confirm the alteration of NSCs. Our data showed that let7a play important roles in controlling NSC fate determination. Thus, manipulating Let-7A and TLX could be a novel strategy to enhance the efficiency of NSC's neuronal differentiation for CNS disorders.

authors

Song J,Cho KJ,Oh Y,Lee JE

doi

10.1016/j.bbrc.2015.05.004

subject

Has Abstract

pub_date

2015-07-10 00:00:00

pages

396-401

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)00894-3

journal_volume

462

pub_type

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