Metabolism of lipoxins A4 and B4 and of their all-trans isomers by human leukocytes and rat liver microsomes.

abstract：:Voltage-dependent anion channel (VDAC) is a multi-functional channel protein in the mitochondrial outer membrane of all eukaryotes. It is involved in extensive physiological and pathophysiological processes. However there is only scant information about VDAC in mammalian reproduction, fertility and development in the ...

journal_title：Biochemical and biophysical research communications

authors： Liu B,Zhang W,Wang Z

更新日期：2010-07-09 00:00:00

abstract：:A Golgi enriched fraction from rat brain was prepared. The preparation has no carboxypeptidase activity and is not contaminated with cytosol, mitochondria and lysosomes as judged by marker enzyme activities for these constituents. Associated with the Golgi membranes a putative opioid peptide processing activity was de...

journal_title：Biochemical and biophysical research communications

authors： Demmer W,Brand K

更新日期：1986-07-16 00:00:00

abstract：:Endothelial cells participate in the inflammatory and immune reactions. Endothelial cell activation is a recurrent phenomenon linked to the pathogenesis of diverse human diseases, such as acute and chronic inflammation and cardiovascular disorders. Pro-inflammatory cytokines (e.g., IL-1, TNF) are well-known activators...

journal_title：Biochemical and biophysical research communications

authors： Takehana K,Konishi A,Oonuki A,Noguchi M,Fujita K,Iino Y,Kobayashi T

更新日期：2002-05-10 00:00:00

abstract：:The function and intracellular localisation of the non-catalytic NH(2)-terminal region of focal adhesion kinase (FAK) are unclear. We investigated the targetting of the FAK NH(2)-terminal domain in HEK 293 and epithelial MDCK cells. Exogenous expression of a variety of GFP-fused and epitope-tagged NH(2) terminal domai...

journal_title：Biochemical and biophysical research communications

authors： Stewart A,Ham C,Zachary I

更新日期：2002-11-22 00:00:00

abstract：:Recently, we have shown that the thyrotropin (TSH) binding regions of human thyrotropin receptor (TSHR) reside in two areas within residues 12-44 and 308-344. Serial antisera were raised against four overlapping synthetic peptides representing these two regions of TSHR (peptides 12-30, 24-44, 308-328, and 324-344) and...

journal_title：Biochemical and biophysical research communications

authors： Sakata S,Ogawa T,Matsui I,Manshouri T,Atassi MZ

更新日期：1992-02-14 00:00:00

abstract：:The major form of cytochrome P-450, P-450K-5, was purified from kidney microsomes of untreated male rats with high-performance liquid chromatography with anion-exchange and hydroxylapatite columns. The monomeric molecular weight of P-450K-5 was 52000 on SDS-polyacrylamide gel electrophoresis and the CO-reduced absorpt...

journal_title：Biochemical and biophysical research communications

authors： Imaoka S,Funae Y

更新日期：1986-12-15 00:00:00

abstract：:The characteristics of carcinoembryonic antigen (CEA) molecules expressed by different cell types have been examined using cDNA transfection into cells of epithelial, neuroectodermal and hematopoietic origin. Variability in the CEA molecules was observed in terms of monoclonal antibody binding, apparent molecular weig...

journal_title：Biochemical and biophysical research communications

authors： Grimm T,Riethmüller G,Johnson JP

更新日期：1994-11-15 00:00:00

abstract：:Arsenic trioxide (ATO) is remarkably effective for treating acute promyelocytic leukemia. Here, we find that ATO treatment of NB4 and K562 leukemic cells induces activation of ASK1. ASK1 activation was induced most significantly at low concentrations of ATO, where G2/M arrest but not apoptosis was induced. On the othe...

journal_title：Biochemical and biophysical research communications

authors： Yan W,Arai A,Aoki M,Ichijo H,Miura O

更新日期：2007-04-20 00:00:00

abstract：:The alpha-subunit secreted by Chang human liver cells was labeled in vitro with [35S]Met, [3H]GlcN, or [3H]Fuc, and the biosynthetic products were studied by SDS-PAGE. Cells labeled with [35S]Met secreted a homogeneous 23K-24K alpha. In contrast, alpha secreted from cells labeled with [3H]GlcN and [3H]Fuc in the absen...

journal_title：Biochemical and biophysical research communications

authors： Morrow JS,Weintraub BD,Rosen SW

更新日期：1983-04-15 00:00:00

abstract：:Cyclin-dependent kinase-like 5 (CDKL5) is a Ser/Thr protein kinase primarily expressed in the central nervous system and is known to cause X-linked neurodevelopmental disorders such as Rett syndrome. However, the mechanisms regulating CDKL5 have not yet been fully clarified. Therefore, in this study, we investigated t...

journal_title：Biochemical and biophysical research communications

authors： Oi A,Katayama S,Hatano N,Sugiyama Y,Kameshita I,Sueyoshi N

更新日期：2017-01-08 00:00:00

abstract：:Pyrene, a structural analog of benzo[a]pyrene, is an effective competing ligand for high affinity carcinogen binding proteins in mouse liver. A pyrene-derivatized Sepharose gel was prepared for affinity chromatography purification of these proteins, and adsorbs all detectable [3H]B[a]P-binding activity from hepatic cy...

journal_title：Biochemical and biophysical research communications

authors： Collins S,Altman JD,Marletta MA

更新日期：1985-05-31 00:00:00

abstract：:Sirt1 appear to be NAD(+)-dependent deacetylase that deacetylates histones and several non-histone proteins. In this study, we identified Sirt1 as a physical interaction partner of Tip60, which is a mammalian MYST-type histone acetyl-transferase that specifically acetylates histones H2A and H4. Although Tip60 also ace...

journal_title：Biochemical and biophysical research communications

authors： Yamagata K,Kitabayashi I

更新日期：2009-12-25 00:00:00

abstract：:We tested the hypothesis that adrenomedullin (ADM), a recently isolated hypotensive peptide, is an autocrine transmitter in renal arterial smooth muscle cells (RASMCs). Fura-2 fluorometry revealed that ADM decreases both the cytosolic Ca2+ concentration ([Ca2+]i) and tension induced by 0.3 microM phenylephrine (PE) in...

journal_title：Biochemical and biophysical research communications

authors： Seguchi H,Nishimura J,Kobayashi S,Kumazawa J,Kanaide H

更新日期：1995-10-13 00:00:00

abstract：:We have isolated and characterized the cDNA encoding a novel protein designated DJ-1. DJ-1, sharing no significant homology with the sequences so far reported, did not show transactivation activity in the Gal4 recombinant system, but transformed mouse NIH3T3 cells by itself. Furthermore, DJ-1 showed a cooperative tran...

journal_title：Biochemical and biophysical research communications

authors： Nagakubo D,Taira T,Kitaura H,Ikeda M,Tamai K,Iguchi-Ariga SM,Ariga H

更新日期：1997-02-13 00:00:00

abstract：:Src and Src-family protein-tyrosine kinases are regulatory proteins that play key roles in cell differentiation, motility, proliferation, and survival. The initially described phosphorylation sites of Src include an activating phosphotyrosine 416 that results from autophosphorylation, and an inhibiting phosphotyrosine...

journal_title：Biochemical and biophysical research communications

authors： Roskoski R Jr

更新日期：2005-05-27 00:00:00

abstract：:Vinculin and meta-vinculin are related proteins that localize to adherens junctions. To facilitate studies on the biology of these proteins cDNA clones were isolated from a chick embryonic library made in lambda gt 11. Three clones, accounting for 1/2 of the vinculin message, were identified by immunological analyses ...

journal_title：Biochemical and biophysical research communications

authors： Coutu MD,Simon DJ,Brown AE,Craig SW

更新日期：1987-09-15 00:00:00

abstract：:The effects of trace acetylation on the contact sites in peptide-antibody binding have been investigated by using a label selection method. A 13-residue synthetic peptide having three lysines (LKLVEQQNPKVKL) corresponding to sequence position 155-168 of beta 1,4-galactosyltransferase was used in this study. The four a...

journal_title：Biochemical and biophysical research communications

authors： Yadav SP,Rawitch AB

更新日期：1994-12-30 00:00:00

abstract：:We propose that one manifestation of altered sphingolipid metabolism within tumor cells may be a reduced sensitivity to anti-cancer therapies because of an inability to produce a sufficient apoptotic signal via sphingomyelin hydrolysis to ceramide. If so, then sphingomyelin administration could reverse this effect and...

journal_title：Biochemical and biophysical research communications

authors： Modrak DE,Lew W,Goldenberg DM,Blumenthal R

更新日期：2000-02-16 00:00:00

abstract：:During screening for the flavonoid chemosensitizers, it was found that 5,7,3',4',5'-pentamethoxyflavone (PMF) was equipotent to verapamil in vitro with respect to the chemosensitizing effect. PMF appears to have a chemosensitizing effect not only by increasing the intracellular accumulation of the drugs without compet...

journal_title：Biochemical and biophysical research communications

authors： Choi CH,Kim JH,Kim SH

更新日期：2004-07-30 00:00:00

abstract：:Betulinic acid (BA) exhibits various biological activities such as anti-bacterial, anti-inflammatory, anti-human papilloma virus (HPV), and anti-cancer activities. HPV infection is associated with a high risk of cervical cancer, which is the leading cause of deaths among women worldwide. Therefore, BA is an attractive...

journal_title：Biochemical and biophysical research communications

authors： Kim HJ,Cho HS,Ban HS,Nakamura H

更新日期：2020-03-12 00:00:00

abstract：:The amino acid sequence of the polyclonal antibodies we developed against the carboxyl terminus of the dystrophin-related protein, the putative gene product of B3 cDNA, had no homologous sequence to the dystrophin molecule except for two amino acids located at its ends for immunization. By immunohistochemical examinat...

journal_title：Biochemical and biophysical research communications

authors： Takemitsu M,Ishiura S,Koga R,Kamakura K,Arahata K,Nonaka I,Sugita H

更新日期：1991-11-14 00:00:00

abstract：:The involvement of vitamin D in prostate carcinogenesis was investigated using the human prostatic LNCaP cells. Incubation of the LNCaP with 100 nM 1 alpha,25-dihydroxyvitamin D3 for 2 days resulted in a 30-40% suppression of cell growth, which was accompanied by a greater than 70% down-regulated expression of the pro...

journal_title：Biochemical and biophysical research communications

authors： Hsieh TY,Ng CY,Mallouh C,Tazaki H,Wu JM

更新日期：1996-06-05 00:00:00

abstract：:By dimerizing with E2F proteins, TFDP has profound influence on cellular E2F activities. While TFDP1 and 2 enhance the DNA binding and the transcriptional activity of E2F, the newly identified member of the DP family, TFDP3 primarily functions as a negative regulator. To further characterize the inhibitory property of...

journal_title：Biochemical and biophysical research communications

authors： Tian C,Lv D,Qiao H,Zhang J,Yin YH,Qian XP,Wang YP,Zhang Y,Chen WF

更新日期：2007-09-14 00:00:00

abstract：:The Cardiac sodium channel gene SCN5A plays a critical role in cardiac electrophysiology and its mutations, either gain- or loss-of-functions, are associated with lethal arrhythmias. In this study, we investigated the effect of overexpression of SCN5A on the cardiac phenotype in a transgenic mouse model (TG-WT L10). C...

journal_title：Biochemical and biophysical research communications

authors： Zhang T,Yong SL,Tian XL,Wang QK

更新日期：2007-04-06 00:00:00

abstract：:A new protein observed in two-dimensional electrophoresis patterns of proteins from the human skin fibroblast line GM1386 has been identified as a charge and molecular-weight variant of the type of tropomyosin found in smooth muscle (Tm:3). This is the second variant of Tm:3 found in GM1386 and represents a second sit...

journal_title：Biochemical and biophysical research communications

authors： Giometti CS,Gemmell MA,Anderson NL

更新日期：1985-05-16 00:00:00

abstract：:Maturity-onset diabetes of the young (MODY) is an autosomal dominant form of diabetes characterized by early onset of pancreatic dysfunction. MODY type 3 is caused by mutations in the hepatocyte nuclear factor (HNF)-1alpha. During a screening of Norwegian patients with suspected MODY we identified two novel HNF-1alpha...

journal_title：Biochemical and biophysical research communications

authors： Bjørkhaug L,Ye H,Horikawa Y,Søvik O,Molven A,Njølstad PR

更新日期：2000-12-29 00:00:00

abstract：:Crystallographic studies have shown independent binding sites for sugar and peptide ligands of concanavalin A, although they were considered functional mimics based on biochemical experiments. The topological correlation of 12-residue peptide with different carbohydrate ligands of concanavalin A showed similarity betw...

journal_title：Biochemical and biophysical research communications

authors： Jain D,Kaur KJ,Goel M,Salunke DM

更新日期：2000-06-16 00:00:00

abstract：:The molecular mechanisms by which chronic hypoxia, whether constant (CCH) or intermittent (CIH), alters the heart rhythm are still under debate. Expression level, control, maturational profile and intercoordination of 54 genes encoding heart rhythm determinants (HRDs) were analyzed in 36 mice subjected for 1, 2 or 4 w...

journal_title：Biochemical and biophysical research communications

authors： Iacobas DA,Iacobas S,Haddad GG

更新日期：2010-01-22 00:00:00

abstract：:Fluorescent timers are useful tools for studying the spatial and temporal cellular or molecular events. Based on the trans-splicing mechanism in Caenorhabditis elegans, we constructed a "fluorescent timer" through bicistronic expression of two fluorescent proteins with different maturation times. When used in vivo, th...

journal_title：Biochemical and biophysical research communications

authors： Chen MR,Yang S,Niu WP,Li ZY,Meng LF,Wu ZX

更新日期：2010-04-23 00:00:00

abstract：:The mechanism(s) by which HIV-1 infection contributes to depletion of CD4(+) T cell is not well understood. In this report, we investigated whether a recently identified isoform of growth factor receptor bound protein (Grb2), named Grb3-3, a signaling molecule that is associated with the MAP kinase pathway and with ap...

journal_title：Biochemical and biophysical research communications

authors： Li X,Multon MC,Henin Y,Schweighoffer F,Venot C,LaVecchio J,Josef J,Stuckert P,Mhashilkar A,Tocqué B,Marasco WA

更新日期：2000-09-16 00:00:00