Biological activities of rabbit antibodies against synthetic human thyrotropin receptor peptides representing thyrotropin binding regions.

abstract：:In our previous paper, we reported that a monoclonal antibody (MAb) 710F, which recognizes a cell surface glycoprotein, induces morphological change in monocytic cells, promoting cellular adhesion and extensive spreading on tissue culture dishes [Koyama et al., Biochem, Biophys. Res. Commun., 168 (1990) 898-904]. We r...

journal_title：Biochemical and biophysical research communications

authors： Koyama Y,Suzuki M,Yoshida T

更新日期：1998-05-29 00:00:00

abstract：:The fluorescence decay of adenosine in 1:1 glycol/water glass has been determined at 77K using narrow pulse (700 ps) laser excitation at 290 nm and fluorescence detection with a scanned narrow-gate (100 ps) fast sampler together with digital averaging. Data analysis by re-iterative non-linear least squares convolution...

journal_title：Biochemical and biophysical research communications

authors： Hart LP,Daniels M

更新日期：1989-07-31 00:00:00

abstract：:It has been demonstrated that joint cultivation of human K-562 cells and mouse L-929 cells results in triggering apoptosis in L-929 cells and possibly also in K-562 cells. Analysis of proteins recovered from the culture medium has revealed the presence of three cytotoxic factors including TNF-alpha. This is the first ...

journal_title：Biochemical and biophysical research communications

authors： Gnuchev NV,Sashchenko LP,Iarovaia OV,Dukhanina E,Lukianova T,Razin SV

更新日期：1997-05-29 00:00:00

abstract：:Although mTOR (mammalian target of rapamycin) activation is frequently observed in acute myeloid leukemia (AML) patients, the precise function and the downstream targets of mTOR are poorly understood. Here we revealed that PFKFB3, but not PFKFB1, PFKFB2 nor PFKFB4 was a novel downstream substrate of mTOR signaling pat...

journal_title：Biochemical and biophysical research communications

authors： Feng Y,Wu L

更新日期：2017-02-05 00:00:00

abstract：:Proteolipid protein, the major protein component of central nervous system (CNS) myelin, is known to contain approximately 2% by weight of covalently bound fatty acids. Recently, this protein was also found, though at a lower concentration, in the peripheral nervous system (PNS). The present study was undertaken to de...

journal_title：Biochemical and biophysical research communications

authors： Tetzloff SU,Bizzozero OA

更新日期：1993-06-30 00:00:00

abstract：:Plastocyanin (PC) is a copper protein that serves as a mobile electron carrier between cytochrome f and Photosystem I in the light reactions of photosynthesis. Despite large variability in amino acid sequences and isoelectric points, PCs from cyanobacterial and chloroplast sources reveal considerable similarities with...

journal_title：Biochemical and biophysical research communications

authors： Babu CR,Arudchandran A,Hille R,Gross EL,Bullerjahn GS

更新日期：1997-06-27 00:00:00

abstract：:Metabolic assay buffers often omit bicarbonate, which is susceptible to alkalinisation in an open environment. Here, we assessed the effect of including bicarbonate in respirometry experiments. By supplementing HEPES-buffered media with low concentrations of bicarbonate, we found increased respiration in adipocytes an...

journal_title：Biochemical and biophysical research communications

authors： Krycer JR,Fisher-Wellman KH,Fazakerley DJ,Muoio DM,James DE

更新日期：2017-08-05 00:00:00

abstract：:Most of the ADP-glucose pyrophosphorylases from different sources are stable to a heat treatment. We found that in the potato (Solanum tuberosum L.) tuber enzyme, the intermolecular disulfide bridge located between Cys12 of the small subunits is responsible for the stability at 60 degrees C. When this unique disulfide...

journal_title：Biochemical and biophysical research communications

authors： Ballicora MA,Fu Y,Frueauf JB,Preiss J

更新日期：1999-04-21 00:00:00

abstract：:Human platelets are less responsive to PAF at 37 degrees than at 25 degrees. They can be desensitized to the effects of PAF by pre-exposure to small concentrations. In both cases desensitization appears to be accompanied by a decreased affinity of the high affinity site for PAF rather than loss of binding sites. Alter...

journal_title：Biochemical and biophysical research communications

authors： Chesney CM,Pifer DD,Huch KM

更新日期：1985-02-28 00:00:00

abstract：:Chloroplast DNA is bound to the thylakoids of spinach chloroplasts. To examine a possible role for thylakoid-bound DNA in chloroplast DNA replication, vesicles formed by treating chloroplasts in 3.5 mM MgCl2 were used. Chloroplast DNA fragments are bound to the surface of these vesicles. Chloroplast DNA isolated from ...

journal_title：Biochemical and biophysical research communications

authors： Lindbeck AG,Rose RJ

更新日期：1990-10-15 00:00:00

abstract：:We isolated and sequenced a cDNA clone corresponding to the entire coding sequence of rat liver lysosomal cathepsin D. The deduced amino acid sequence revealed that cathepsin D consists of 407 amino acid residues (Mr 44,608) and the 20 NH2-terminal residues seem to constitute a cleavable signal peptide after which 44 ...

journal_title：Biochemical and biophysical research communications

authors： Fujita H,Tanaka Y,Noguchi Y,Kono A,Himeno M,Kato K

更新日期：1991-08-30 00:00:00

abstract：:We identified 13 transcript isoforms of a trinucleotide-repeat-containing gene, cag-8, that is expressed almost exclusively in the mouse brain. The polypeptide deduced from the cDNA consists of 137 AAs, of which 74 are glutamines. The 130-kb gene is composed of 16 exons, from which at least 13 isoforms with variable U...

journal_title：Biochemical and biophysical research communications

authors： Ji JW,Yang HL,Kim SJ

更新日期：2006-08-11 00:00:00

abstract：:Under many physiological circumstances, Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high-affinity choline uptake (HACU) in cholinergic neurons is thought to be rate-limiting in the biosynthesis of acetylcholine (ACh). Based on sequence information provided by the Human Genome Project and the recently...

journal_title：Biochemical and biophysical research communications

authors： Apparsundaram S,Ferguson SM,George AL Jr,Blakely RD

更新日期：2000-10-05 00:00:00

abstract：:Disordered circadian rhythms are associated with various psychiatric conditions and metabolic diseases. We recently established a mouse model of a psychophysiological stress-induced chronic sleep disorder (CSD) characterized by reduced amplitude of circadian wheel-running activity and sleep-wake cycles, sleep fragment...

journal_title：Biochemical and biophysical research communications

authors： Oishi K,Yamamoto S,Itoh N,Miyazaki K,Nemoto T,Nakakita Y,Kaneda H

更新日期：2014-07-18 00:00:00

abstract：:Integral membrane lysophospholipid acyltransferases (AT) are involved in many reactions that produce phospholipids and triglycerides. Enzymes that utilize lysophosphatidic acid (LPA) as an acceptor substrate have been termed LPAATs, and several are members of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) ge...

journal_title：Biochemical and biophysical research communications

authors： Schmidt JA,Yvone GM,Brown WJ

更新日期：2010-07-09 00:00:00

abstract：:In malate dehydrogenase from Thermus flavus, substitution of Ile for Thr189, which is involved in hydrogen-bond network around a loop containing catalytic His186, caused an increase in the turnover number at room temperatures. In order to examine the effect of disruption of the hydrogen bonds on the catalytic activity...

journal_title：Biochemical and biophysical research communications

authors： Nishiyama M,Kinoshita M,Kudo H,Horinouchi S,Tanokura M

更新日期：1996-08-23 00:00:00

abstract：:Even though earlier reports have revealed that Aquaporin 8 (AQP8) exert essential roles in diverse malignancies, its relationship between specific microRNAs (miRNAs) in regulation of colorectal carcinoma (CRC) progression has never been elaborated. Herein, we proved that AQP8 was downregulated in CRC and high level of...

journal_title：Biochemical and biophysical research communications

authors： Zhang H,Du WB,Guo XM,Wang LK,Cheng JM,Wei LJ

更新日期：2020-06-18 00:00:00

abstract：:Different progestogens are widely used in hormonal therapy and mediate their therapeutic actions via the progesterone receptor (PR). Little published data exist on their relative efficacies and potencies via the PR, while those available may be confounded by off-target receptors, different methodologies and model syst...

journal_title：Biochemical and biophysical research communications

authors： Enfield K,Cartwright M,Toit RL,Avenant C,Africander D,Hapgood JP

更新日期：2020-12-17 00:00:00

abstract：:Retinoids induce marked growth inhibition and neuritic differentiation in human neuroblastoma cells. Expression patterns of nuclear retinoic acid receptors (RAR) in embryonic and adult tissues suggests that RAR subtypes alpha, beta and gamma have tissue-specific functions. We have transfected a human neuroblastoma tum...

journal_title：Biochemical and biophysical research communications

authors： Cheung B,Hocker JE,Smith SA,Reichert U,Norris MD,Haber M,Stewart BW,Marshall GM

更新日期：1996-12-04 00:00:00

abstract：:The DNA-dependent DNA polymerase (DDDP) and RNA-dependent DNA polymerase (RDDP) activities of hepadnavirus polymerases are both essential for viral replication. Human hepatitis B virus (HBV) polymerase has been successfully expressed in Escherichia coli as a fusion protein in frame with maltose-binding protein. The pr...

journal_title：Biochemical and biophysical research communications

authors： Jeong JH,Kwak DS,Rho HM,Jung G

更新日期：1996-06-14 00:00:00

abstract：:In the present study, we tested the anti-pancreatic cancer activity by AT406, a small-molecule antagonist of IAP (inhibitor of apoptosis proteins). In established (Panc-1 and Mia-PaCa-2 lines) and primary human pancreatic cancer cells, treatment of AT406 significantly inhibited cell survival and proliferation. Yet, sa...

journal_title：Biochemical and biophysical research communications

authors： Jiang Y,Meng Q,Chen B,Shen H,Yan B,Sun B

更新日期：2016-09-09 00:00:00

abstract：:The activity of Cav1.2 Ca(2+) channels is maintained in the presence of calmodulin and ATP, even in cell-free patches, and thus a channel ATP-binding site has been suggested. In this study, we examined whether other nucleotides, such as GTP, UTP, CTP, ADP and AMP, could be substituted for ATP in guinea-pig ventricular...

journal_title：Biochemical and biophysical research communications

authors： Liu SY,Xu JJ,Minobe E,Gao QH,Feng R,Zhao MM,Guo F,Yang L,Hao LY,Kameyama M

更新日期：2015-05-08 00:00:00

abstract：:In this study, we use HRMAS NMR as a non-invasive technique to monitor the in vivo metabolism of a xenobiotic. The antituberculosis Ethionamide is a pro-drug that has to be activated in mycobacteria before inhibiting its cellular target. The use of (1)H HRMAS NMR has allowed to detect a metabolite (ETH*) of the drug d...

journal_title：Biochemical and biophysical research communications

authors： Hanoulle X,Wieruszeski JM,Rousselot-Pailley P,Landrieu I,Baulard AR,Lippens G

更新日期：2005-06-03 00:00:00

abstract：:The aim of this study was to determine molecular mechanism(s) responsible for the reduced thymidine kinase activity (TK) observed earlier in an arabinosylcytosine (araC) resistant lymphoid cell line (H9-araC cells), which was obtained following continuous cultivation of H9 cells in the presence of 0.5 microM araC. Com...

journal_title：Biochemical and biophysical research communications

authors： Han T,Fernandez M,Sarkar M,Agarwal RP

更新日期：2003-08-01 00:00:00

abstract：:An orphan G protein-coupled receptor, termed 6H1, with approximately 30% sequence identity to P2Y receptors has been proposed to be a P2Y receptor (p2y5) based solely on a radioligand binding assay with [35S]dATP alphaS [Webb et al. (1997) Biochem. Biophys. Res. Commun. 219:105-110]. Previous work in our laboratory ha...

journal_title：Biochemical and biophysical research communications

authors： Li Q,Schachter JB,Harden TK,Nicholas RA

更新日期：1997-07-18 00:00:00

abstract：:An essential pre-requisite to perform sound quantitative real-time polymerase chain reaction (qPCR) assays is to design outstanding primer pairs. This means they must have a good efficiency and be not prone to produce multiple amplicons or primer dimer products. To circumvent these issues, several softwares are availa...

journal_title：Biochemical and biophysical research communications

authors： Nonis A,Scortegagna M,Nonis A,Ruperti B

更新日期：2011-12-02 00:00:00

abstract：:K+ channels are integral membrane proteins, which contribute to maintain vital parameters such as the cellular membrane potential and cell volume. Malaria parasites encode two K+ channel homologues, Kch1 and Kch2, which are well-conserved among members of the Plasmodium genus. In the rodent malaria parasite P. berghei...

journal_title：Biochemical and biophysical research communications

authors： Ellekvist P,Mlambo G,Kumar N,Klaerke DA

更新日期：2017-11-04 00:00:00

abstract：:MS-209 is a novel quinoline derivative reversing P-glycoprotein-mediated multidrug resistance (MDR). We investigated the interaction between MS-209 and multidrug resistance protein (MRP) in MRP-overexpressing human gastric cancer cells. We measured [3H]leukotriene C4 uptake into the membrane vesicles of the cells and ...

journal_title：Biochemical and biophysical research communications

authors： Nakamura T,Oka M,Aizawa K,Soda H,Fukuda M,Terashi K,Ikeda K,Mizuta Y,Noguchi Y,Kimura Y,Tsuruo T,Kohno S

更新日期：1999-02-24 00:00:00

abstract：:The genomes of several vertebrates contain two genes encoding proteins highly similar to threonine synthase (TS), even though the biosynthesis of l-threonine (l-Thr) is not known to occur in these animals. We report a bioinformatic analysis of the two TS-like genes, the recombinant expression of one murine TS homolog ...

journal_title：Biochemical and biophysical research communications

authors： Donini S,Percudani R,Credali A,Montanini B,Sartori A,Peracchi A

更新日期：2006-12-01 00:00:00

abstract：:Hemoglobin (Hb) isolated from the backswimmer Buenoa margaritacea has been analyzed spectroscopically. The met form at pH less than 6 shows a 30nm red shift in the Qv and Qo bands and a 5nm red shift in the Soret band compared to mammalian Hb, while only minor differences are seen in the spectra of the CO and O2 adduc...

journal_title：Biochemical and biophysical research communications

authors： Osmulski PA,Vossbrinck CR,Sampath V,Caughey WS,Debrunner PG

更新日期：1992-09-16 00:00:00