The 6H1 orphan receptor, claimed to be the p2y5 receptor, does not mediate nucleotide-promoted second messenger responses.

abstract：:Protein L7 is associated with the large subunit of eukaryotic ribosomes that can act as a co-regulator of nuclear receptor-mediated transcription. In this study we show that L7 carries in addition to the known N-terminal nucleic acid-binding domain (NBD 1) a second one (NBD 2) which maps to the 50 C-terminal amino aci...

journal_title：Biochemical and biophysical research communications

authors： von Mikecz A,Neu E,Krawinkel U,Hemmerich P

更新日期：1999-05-19 00:00:00

abstract：:Ligand-blotting and dot-blotting procedures were used to investigate the binding of [125I]-heparin to apolipoprotein E, its thrombin fragments E22 (residues 1-191) and E12 (residues 192-299), and to nine apolipoprotein E synthetic fragments. E22 and E12 bound [125I] heparin indicating multiple heparin-binding domains....

journal_title：Biochemical and biophysical research communications

authors： Cardin AD,Hirose N,Blankenship DT,Jackson RL,Harmony JA,Sparrow DA,Sparrow JT

更新日期：1986-01-29 00:00:00

abstract：:The previous study showed involvement of spinal glia in tetanically sciatic stimulation-induced long-term potentiation (LTP) of C-fiber-evoked field potentials in the spinal dorsal horn. In the present study, the electrophysiological recording and paw withdrawal threshold (PWT) to von Frey stimulation were assessed fo...

journal_title：Biochemical and biophysical research communications

authors： Ying B,Lü N,Zhang YQ,Zhao ZQ

更新日期：2006-02-24 00:00:00

abstract：:Most human cancers are of epithelial origin, but many cell culture models for the study of cancer-causing genes use fibroblasts. In addition, efficient delivery and stable expression of foreign genes into non-transformed cell lines are often difficult. To address both questions, we here established a non-transformed r...

journal_title：Biochemical and biophysical research communications

authors： Fu SL,Huang YJ,Liang FP,Huang YF,Chuang CF,Wang SW,Yao JW

更新日期：2005-12-16 00:00:00

abstract：:The products of the human hepatitis B virus (HBV) and woodchuck hepatitis B virus X genes (pXs) transactivate homologous and heterologous genes including the HBV-X and core promoters, the human immunodeficiency viruses 1 (HIV-1) and 2 (HIV-2) long terminal repeats and the beta interferon regulatory sequences. We repor...

journal_title：Biochemical and biophysical research communications

authors： Balsano C,Avantaggiati ML,Natoli G,De Marzio E,Will H,Perricaudet M,Levrero M

更新日期：1991-05-15 00:00:00

abstract：:Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration...

journal_title：Biochemical and biophysical research communications

authors： Baumann U,Frank R,Blöcker H

更新日期：1988-12-30 00:00:00

abstract：:Signaling pathways for the antiviral and antiproliferative biological effects of type I interferons (IFN) are well established. In this report we demonstrate a novel signaling pathway for IFN-alpha, as it induced rapid phosphorylation of both PKB/Akt and its substrate forkhead. The PI3-kinase inhibitor LY294002 abolis...

journal_title：Biochemical and biophysical research communications

authors： Ruuth K,Carlsson L,Hallberg B,Lundgren E

更新日期：2001-06-15 00:00:00

abstract：:NADPH oxidases (NOX) - derived reactive oxygen species (ROS) contribute to oxidative injury in hypoxia-induced pulmonary arterial hypertension. This study aims to evaluate the status of NOX in endothelial progenitor cells (EPCs) under hypoxic condition and to determine whether NOX inhibitors could attenuate hypoxia-in...

journal_title：Biochemical and biophysical research communications

authors： Liu B,Ren KD,Peng JJ,Li T,Luo XJ,Fan C,Yang JF,Peng J

更新日期：2017-01-22 00:00:00

abstract：:The effect of vitamin A on the expression of apoAI and CIII genes in intact rats was studied. In vitamin A-deficient rats, the hepatic level of apoAI mRNA was increased and enhanced by an oral administration of excess retinoic acid(RA). A similar administration to normal rats caused an increase in the apoAI mRNA level...

journal_title：Biochemical and biophysical research communications

authors： Nagasaki A,Kikuchi T,Kurata K,Masushige S,Hasegawa T,Kato S

更新日期：1994-12-30 00:00:00

abstract：INTRODUCTION:SIRT2 is a NAD(+)-dependent deacetylases and associated with numerous processes such as infection, carcinogenesis, DNA damage and cell cycle regulation. However, the role of SIRT2 in inflammatory process in macrophage remains unclear. MATERIALS AND METHODS:In the present study, we have evaluated the regul...

journal_title：Biochemical and biophysical research communications

authors： Lee AS,Jung YJ,Kim D,Nguyen-Thanh T,Kang KP,Lee S,Park SK,Kim W

更新日期：2014-08-08 00:00:00

abstract：:The baculovirus expression system has been used to express large quantities of various proteins, including membrane receptors. Here, we reveal a novel property of this expression system to be that certain membrane proteins can be displayed on the budded virus itself. We introduced the genes encoding sterol regulatory ...

journal_title：Biochemical and biophysical research communications

authors： Urano Y,Yamaguchi M,Fukuda R,Masuda K,Takahashi K,Uchiyama Y,Iwanari H,Jiang SY,Naito M,Kodama T,Hamakubo T

更新日期：2003-08-15 00:00:00

abstract：:Based on a homology search with endostatin, the C-terminus 185 aa of collagen XVIII, we report the cloning, expression, and antiangiogenic activity of a 22 kDa human collagen XV fragment, that we have named restin. Restin was expressed in the prokaryotic pET expression system. We have shown that restin inhibits the mi...

journal_title：Biochemical and biophysical research communications

authors： Ramchandran R,Dhanabal M,Volk R,Waterman MJ,Segal M,Lu H,Knebelmann B,Sukhatme VP

更新日期：1999-02-24 00:00:00

abstract：:The role of mycothiol in mycobacteria was examined by comparative analysis of mutants disrupted in the four known genes encoding the protein machinery needed for mycothiol biosynthesis. These mutants were sensitive to acid stress, antibiotic stress, alkylating stress, and oxidative stress indicating that mycothiol and...

journal_title：Biochemical and biophysical research communications

authors： Rawat M,Johnson C,Cadiz V,Av-Gay Y

更新日期：2007-11-09 00:00:00

abstract：:Two polypeptides are involved in interleukin 2 binding: a low-affinity receptor of 55 kD (IL2-R alpha) and an intermediate affinity component of 75 kD (IL2-R beta). We describe the cloning by the Polymerase Chain Reaction of the coding region of IL2-R alpha from a human T-cell lymphoma cell line. One clone presented a...

journal_title：Biochemical and biophysical research communications

authors： Mercken L,Moras V,Hemon L,Lionne B,Bousseau A,Dautry-Varsat A,Collins M,Mayaux JF

更新日期：1991-11-14 00:00:00

abstract：INTRODUCTION:Radiotherapy is an equivalent alternative or complement to radical prostatectomy, with high therapeutic efficacy. High risk patients, however, experience high relapse rates, so that research on radio-sensitization is the most evident route to improve curability of this common disease. MATERIALS AND METHOD...

journal_title：Biochemical and biophysical research communications

authors： Koukourakis MI,Kalamida D,Mitrakas A,Pouliliou S,Kalamida S,Sivridis E,Giatromanolaki A

更新日期：2015-05-29 00:00:00

abstract：:Glycosaminoglycans were prepared from the Engelbreth-Holm-Swarm mouse tumor. Enzymatic analysis demonstrated heparan sulfate (95.8%) and chondroitinase ABC-sensitive galactosaminoglycans (4.2%). HPLC analysis of the disaccharide units showed that heparan sulfate chains were undersulfated on average, comprising approxi...

journal_title：Biochemical and biophysical research communications

authors： Sugahara K,Okumura Y,Yamashina I

更新日期：1989-07-14 00:00:00

abstract：:Nonalcoholic fatty liver disease (NAFLD) represents a global health problem. Impaired autophagy has been implicated in the pathogenesis of NAFLD, and CD147 is recognized to regulate lipid metabolism in a variety of cell types. This study was initiated with the aim to identify molecular makers expressed in hepatocytes ...

journal_title：Biochemical and biophysical research communications

authors： Lou J,Li C,Li ZS,Zhang T,Chen ZN,Bian H

更新日期：2020-04-16 00:00:00

abstract：:Transmissible gastroenteritis virus (TGEV), an enteropathogenic coronavirus, causes severe lethal watery diarrhea and dehydration in piglets. Previous studies indicate that TGEV infection induces cell apoptosis in host cells. In this study, we investigated the roles and regulation of reactive oxygen species (ROS) in T...

journal_title：Biochemical and biophysical research communications

authors： Ding L,Zhao X,Huang Y,Du Q,Dong F,Zhang H,Song X,Zhang W,Tong D

更新日期：2013-12-06 00:00:00

abstract：:The frequency of p73 mutation is low in hematologic malignancies as well as solid tumors. Aberrant DNA methylation of multiple promoter associated CpG islands is a frequent phenomenon in acute lymphoblastic leukemia (ALL). In the present study, we scanned for mutations in the exons 4, 5, 6, and 7 of p73 gene. Using PC...

journal_title：Biochemical and biophysical research communications

authors： Sahu GR,Das BR

更新日期：2005-02-18 00:00:00

abstract：:Tyrosine hydroxylase (TH) is the rate-limiting enzyme in catecholamine biosynthesis, and its N-terminus plays a critical role in the intracellular stability of the enzyme. In the present study, we investigated the mechanism by which the N-terminal region of TH affects this stability. TH molecules phosphorylated at the...

journal_title：Biochemical and biophysical research communications

authors： Nakashima A,Mori K,Kaneko YS,Hayashi N,Nagatsu T,Ota A

更新日期：2011-04-08 00:00:00

abstract：:Allylisothiocyanate (ANCS) is shown to inhibit (H+ + K+)-ATPase isolated from the parietal cell of hog gastric mucosa. The ATPase is the proton pump in the secretory membrane of the parietal cell and is an essential component of the acid secretory mechanism. Furthermore, ANCS suppresses acid secretion by bullfrog gast...

journal_title：Biochemical and biophysical research communications

authors： Takeguchi N,Nishimura Y,Watanabe T,Mori Y,Morii M

更新日期：1983-04-29 00:00:00

abstract：:The diagnostic significance of phosphoglycerate mutase (PGM) B-type isozyme activity capable of being inducible under hypoxia at the gene level as a serum marker for cerebral stroke was investigated. The normal level (mean +/- 2 SD) in human serum was determined to be 38 +/- 18 units/L. Within 2 h after the onset of c...

journal_title：Biochemical and biophysical research communications

authors： Hayashi T,Matuo Y

更新日期：2001-10-05 00:00:00

abstract：:Rett syndrome is a neurodevelopmental disorder caused by Mecp2 gene mutations. In RTT patients and Mecp2-null (Mecp2(-/Y)) mice, norepinephrine (NE) content drops significantly, which may play a role in breathing arrhythmia, sleep disorders and sudden death. However, the underlying mechanisms for the NE defect are not...

journal_title：Biochemical and biophysical research communications

authors： Zhang X,Su J,Rojas A,Jiang C

更新日期：2010-04-02 00:00:00

abstract：:We have studied the oligosaccharide chains of the variant surface glycoprotein (VSG) of Trypanosoma brucei brucei MITat 1.6. Glycopeptides were generated by Pronase digestion, purified by gel permeation and ion-exchange chromatography, and structurally characterized by 1H and 31P NMR spectroscopy in combination with c...

journal_title：Biochemical and biophysical research communications

authors： Strang AM,Allen AK,Holder AA,van Halbeek H

更新日期：1993-11-15 00:00:00

abstract：:Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal of subunit III, su...

journal_title：Biochemical and biophysical research communications

authors： Yewey GL,Caughey WS

更新日期：1987-11-13 00:00:00

abstract：:Up to 1 mol of phosphoryl groups was incorporated per mol of eukaryotic protein synthesis initiation factor (eIF) 4E following incubation of purified preparations of this factor with purified preparations of a protamine kinase from bovine kidney cytosol. By contrast, purified preparations of two forms of mitogen-activ...

journal_title：Biochemical and biophysical research communications

authors： Amick GD,Damuni Z

更新日期：1992-03-16 00:00:00

abstract：:Hydrogen sulfide (H2S), a gaseous signaling molecule produced by cystathionine γ-lyase (CSE) and cystathionine β-synthase (CBS), influences bone remodeling in many ways. Osteoclasts play an important role in bone remodeling during tooth movement induced by mechanical loading, which increases the rate of tooth movement...

journal_title：Biochemical and biophysical research communications

authors： Mo S,Hua Y

更新日期：2018-06-22 00:00:00

abstract：:12-O-tetradecanoyl phorbol 13-acetate (TPA) and 1,2-dioctanoyl-sn-glycerol (diC8) activate protein kinase C (PKC) in transformed fetal bovine aortic endothelial GM 7373 cells. Both molecules cause a similar increase in membrane-associated PKC activity and in the phosphorylation of a PKC-specific endogenous 87-kDa subs...

journal_title：Biochemical and biophysical research communications

authors： Maier JA,Presta M,Ragnotti G

更新日期：1989-04-28 00:00:00

abstract：:Kaiso was previously described as a methylated DNA-binding protein and a transcription repressor interacting with the corepressor protein complex NCoR. In the current study, we show that generation-3 Kaiso knockout mice show a phenotype of splenomegaly and large diffused germinal centers (GC). In the spleens of Kaiso ...

journal_title：Biochemical and biophysical research communications

authors： Koh DI,Yoon JH,Kim MK,An H,Kim MY,Hur MW

更新日期：2013-12-13 00:00:00

abstract：:The activation of caspase-3 is a key surrogate marker for detecting apoptosis. To quantitate caspase-3 activity, we constructed a biosensor comprising a recombinant firefly luciferase containing a caspase-3 cleavage site. When apoptosis was induced, caspase-3 cleavage of the biosensor activated firefly luciferase by a...

journal_title：Biochemical and biophysical research communications

authors： Shi J,Zhang H,Fang L,Xi Y,Zhou Y,Luo R,Wang D,Xiao S,Chen H

更新日期：2014-10-03 00:00:00