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Lifetime evidence for a weak lowest electronic transition in adenosine.

Abstract：

:The fluorescence decay of adenosine in 1:1 glycol/water glass has been determined at 77K using narrow pulse (700 ps) laser excitation at 290 nm and fluorescence detection with a scanned narrow-gate (100 ps) fast sampler together with digital averaging. Data analysis by re-iterative non-linear least squares convolution shows the decay is best represented by the bi-exponential form I(t) = 0.59exp - t/1.2ns + 0.41 exp - t/7.0ns. This leads to intrinsic radiative lifetimes of 150 ns and 220 ns respectively and a combined oscillator strength of 1.5 x 10(-2). Compared with the overall oscillator strength of 0.29 for the entire first absorption band of adenosine this indicates that transitions to and from the lowest-lying state in this band are quite forbidden. This is not accounted for by current theoretical considerations.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hart LP,Daniels M

doi

10.1016/0006-291x(89)92378-4

subject

Has Abstract

pub_date

1989-07-31 00:00:00

pages

781-7

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(89)92378-4

journal_volume

162

pub_type

杂志文章

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