Direct radioimmunoassay of atrial natriuretic factor.

abstract：:The effects of dopamine (DA) on 45Ca2+ ion movement and prolactin release in dispersed female rat anterior pituitary cells were studied to elucidate the mechanism for DA reduction of intracellular calcium levels. In 45Ca2+ prelabeled cells, DA inhibited fractional calcium efflux and prolactin release simultaneously an...

journal_title：Biochemical and biophysical research communications

authors： Login IS,Judd AM,MacLeod RM

更新日期：1988-03-15 00:00:00

abstract：:The peptide endothelin causes a biphasic rise in intracellular free calcium levels in cultured Type-1 astrocytes and C6 glioma cells, suggesting that glial cells may be the physiological target of endothelin in the brain. Endothelin also causes a calcium-dependent increase [3H]thymidine incorporation in primary cultur...

journal_title：Biochemical and biophysical research communications

authors： Supattapone S,Simpson AW,Ashley CC

更新日期：1989-12-29 00:00:00

abstract：:The substrate specificities of monoamine oxidase (MAO) A isolated from human placenta and of human liver expressed in yeast have been compared in homogeneous preparations with respect to Vmax and Km values for natural and synthetic substrates and Ki values for competitive inhibitors. MAO A from these two sources is kn...

journal_title：Biochemical and biophysical research communications

authors： Tan AK,Weyler W,Salach JI,Singer TP

更新日期：1991-12-31 00:00:00

abstract：:The human pregnane X receptor (hPXR) plays a key role in the regulation of both drug metabolism and efflux by inducing the expression of CYP3A4 and MDR1 gene. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we identified seven novel splicing variants of hPXR in tissue from a single human liver...

journal_title：Biochemical and biophysical research communications

authors： Fukuen S,Fukuda T,Matsuda H,Sumida A,Yamamoto I,Inaba T,Azuma J

更新日期：2002-11-01 00:00:00

abstract：:The present study was designed to clarify the cellular localization and expression of leptin receptor(s) [OB-R(s)] mRNA including its splice variants and their correlation with the cells which secrete placental hormone, placental lactogen-II (PL-II), in rat placentae. By in situ hybridization analysis, hybridization s...

journal_title：Biochemical and biophysical research communications

authors： Kawai M,Murakami T,Otani S,Shima K,Yamaguchi M,Kishi K

更新日期：1999-04-13 00:00:00

abstract：:Hexokinase II protein is augmented in denervated skeletal muscle; therefore, we determined if hexokinase II gene transcription rates and mRNA levels are increased with denervation. The right hindlimb skeletal muscles of male rats were denervated while the left hindlimbs were sham operated. Seventy-two h following surg...

journal_title：Biochemical and biophysical research communications

authors： Jones JP,Roberts BR,Tapscott EB,Dohm GL

更新日期：1997-09-08 00:00:00

abstract：:Malondialdehyde measurements have been the major tool for studying relationships between lipid peroxidation and tissue pathology. Recently, we presented a novel gas chromatography-mass spectrometry method for direct detection of phospholipid peroxides with picogram sensitivity based on transesterification of phospholi...

journal_title：Biochemical and biophysical research communications

authors： van Kuijk FJ,Thomas DW,Stephens RJ,Dratz EA

更新日期：1986-08-29 00:00:00

abstract：:Organoids mimicking the formation of the brain cortex have been demonstrated to be powerful tools for developmental studies as well as pathological investigations of brain malformations. Here, we report an integrated approach for the quantification of temporal neural production (neurogenic rate) in organoids derived f...

journal_title：Biochemical and biophysical research communications

authors： Kosodo Y,Suetsugu T,Kobayashi TJ,Matsuzaki F

更新日期：2017-01-29 00:00:00

abstract：:In neuroblastoma N1E 115 cells, carbachol, histamine and PGE1 elevated cyclic GMP content and, induced the efflux of preloaded 45Ca2+, the release of membrane-bound Ca2+ measured by fluorescent CTC, and the increase in [Ca2+]i as measured by Quin 2 fluorescence. The time course of the responses, the absolute requireme...

journal_title：Biochemical and biophysical research communications

authors： Ohsako S,Deguchi T

更新日期：1984-07-18 00:00:00

abstract：:To analyze the mechanisms of mRNA export from the nucleus to the cytoplasm, we have isolated eleven mutants, ptr [poly(A)(+) RNA transport] 1 to 11, which accumulate poly(A)(+) RNA in the nucleus at a nonpermissive temperature in Schizosaccharomyces pombe. Of those, the ptr5-1 mutant shows dots- or a ring-like accumul...

journal_title：Biochemical and biophysical research communications

authors： Watanabe N,Ikeda T,Mizuki F,Tani T

更新日期：2012-02-03 00:00:00

abstract：:Centaurin-alpha(1) was originally described as a binding partner for phosphoinositides. In spite of the presence of a putative ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) domain, no ARF-GAP activity has been attributed to centaurin-alpha(1) so far. Thus the function of this protein remains to be dete...

journal_title：Biochemical and biophysical research communications

authors： Dubois T,Zemlickova E,Howell S,Aitken A

更新日期：2003-02-07 00:00:00

abstract：OBJECTIVE:The aim of this study was to determine whether ATP-binding cassette transporter A1 (ABCA1) was up-regulated by growth differentiation factor-15 (GDF-15) via the phosphoinositide 3-kinase (PI3K)/protein kinase Cζ (PKCζ)/specificity protein 1 (SP1) pathway in THP-1 macrophages. METHODS AND RESULTS:We investiga...

journal_title：Biochemical and biophysical research communications

authors： Wu JF,Wang Y,Zhang M,Tang YY,Wang B,He PP,Lv YC,Ouyang XP,Yao F,Tan YL,Tang SL,Tang DP,Cayabyab FS,Zheng XL,Zhang DW,Zeng GF,Tang CK

更新日期：2014-02-14 00:00:00

abstract：:GALIG, an internal gene to the human galectin-3 gene, encodes two distinct proteins, Mitogaligin and Cytogaligin through translation of a unique mRNA in two overlapping alternative reading frames. When overexpressed GALIG induces apoptosis. In cultured cells, Mitogaligin destabilizes mitochondria membranes through int...

journal_title：Biochemical and biophysical research communications

authors： El Haddad S,Serrano A,Normand T,Robin C,Dubois M,Brulé-Morabito F,Mollet L,Charpentier S,Legrand A

更新日期：2018-01-01 00:00:00

abstract：:Ultraviolet, fluorescence and CD spectral analysis suggested unusual structural features of Rhodotorula gracilis ATCC 90950 DNA. R. gracilis DNA exhibited 13% hyperchromicity at 260 nm as against 26% shown by calf thymus DNA. The biphasic melting curve, one phase between 88-92 degrees C and the other between 92-97 deg...

journal_title：Biochemical and biophysical research communications

authors： Vishnuvardhan D,Divakar S,Joseph R

更新日期：1995-06-26 00:00:00

abstract：:X chromosome inactivation in mammalian females occurs early in embryonic development and renders most genes on the inactive X chromosome transcriptionally silenced. As a consequence, females will display an X chromosomal parent-of-origin mosaiscism with regard to which parental allele that is expressed. Some genes, ho...

journal_title：Biochemical and biophysical research communications

authors： Nordquist N,Oreland L

更新日期：2006-09-22 00:00:00

abstract：:Functional endothelial cells and their progenitors are required for vascular development, adequate vascular function, vascular repair and for cell-based therapies of ischemic diseases. Currently, cell therapy is limited by the low abundance of patient-derived cells and by the functional impairment of autologous endoth...

journal_title：Biochemical and biophysical research communications

authors： Kim J,Eligehausen S,Stehling M,Nikol S,Ko K,Waltenberger J,Klocke R

更新日期：2014-01-10 00:00:00

abstract：:MS-209 is a novel quinoline derivative reversing P-glycoprotein-mediated multidrug resistance (MDR). We investigated the interaction between MS-209 and multidrug resistance protein (MRP) in MRP-overexpressing human gastric cancer cells. We measured [3H]leukotriene C4 uptake into the membrane vesicles of the cells and ...

journal_title：Biochemical and biophysical research communications

authors： Nakamura T,Oka M,Aizawa K,Soda H,Fukuda M,Terashi K,Ikeda K,Mizuta Y,Noguchi Y,Kimura Y,Tsuruo T,Kohno S

更新日期：1999-02-24 00:00:00

abstract：:To examine the effects of a replacement of the proximal or the distal histidine on the structure of hemoglobin (Hb), absorption and circular dichroic (CD) spectra of five species of Hbs M in the visible region were measured. Four Hbs M had a characteristic but a similar absorption spectrum upon amino acid substitution...

journal_title：Biochemical and biophysical research communications

authors： Nagai M,Takama S,Yoneyama Y

更新日期：1985-04-30 00:00:00

abstract：:We have previously identified the region containing transcriptional enhancer in mouse immunoglobulin heavy chain (IgH) gene as an initiation region of DNA replication. The octamer sequence therein has been found to be essential for initiation of DNA replication as well as for transcription. Octamer-binding protein fam...

journal_title：Biochemical and biophysical research communications

authors： Ogawa N,Ariga H,Iguchi-Ariga SM

更新日期：1994-05-16 00:00:00

abstract：:MicroRNAs (miRNAs) can negatively regulate gene expression and also induce or inhibit viral replication. In the present study, we found 10 miRNAs were differentially expressed in a stable HBV-producing cell line (HepG2.2.15) compared with its control cell line (HepG2) by miRNA array analysis. miR-501 was significantly...

journal_title：Biochemical and biophysical research communications

authors： Jin J,Tang S,Xia L,Du R,Xie H,Song J,Fan R,Bi Q,Chen Z,Yang G,Liu J,Shi Y,Fan D

更新日期：2013-01-25 00:00:00

abstract：:The IGF-1 receptor (IGF-1R) and MT1-MMP are synthesized as larger precursor proproteins, which require endoproteolytic activation by the proprotein convertases (PCs) furin/PC5 to gain full biological activity. The aim of this study was to investigate the contribution of PCs to IGF-1R and/or MT1-MMP activation in vascu...

journal_title：Biochemical and biophysical research communications

authors： Stawowy P,Kallisch H,Kilimnik A,Margeta C,Seidah NG,Chrétien M,Fleck E,Graf K

更新日期：2004-08-27 00:00:00

abstract：:The present study examines whether and to what extent the profiles of proteolytic-related genes are altered in atrophying muscle during prolonged food deprivation. Long-term fasted animals exhibited three metabolic phases characterized by changes in lipid and protein utilization. Starvation induced an increase in some...

journal_title：Biochemical and biophysical research communications

authors： Bertile F,Le Maho Y,Raclot T

更新日期：2003-11-28 00:00:00

abstract：:Highly reliable biomarkers for the diagnosis of neurological diseases are not widely available. Here we evaluated a luciferase immunoprecipitation technology (LIPS) for the diagnosis of a CNS autoimmune disorder, stiff-person syndrome (SPS). Analysis by LIPS of 40 sera samples from SPS and control subjects for anti-GA...

journal_title：Biochemical and biophysical research communications

authors： Burbelo PD,Groot S,Dalakas MC,Iadarola MJ

更新日期：2008-02-01 00:00:00

abstract：:Isolated Agrobacterium tumefaciens was exposed to different extremely low frequencies of square amplitude modulated waves (QAMW) from two generators to determine the resonance frequency that causes growth inhibition. The carrier was 10 MHz sine wave with amplitude ±10 Vpp which was modulated by a second wave generator...

journal_title：Biochemical and biophysical research communications

authors： Fadel MA,El-Gebaly RH,Mohamed SA,Abdelbacki AMM

更新日期：2017-12-09 00:00:00

abstract：:The effects of compounds which bind in the DNA minor groove of A.T rich sequences, on bleomycin-catalyzed site-specific DNA cleavage were investigated by a DNA sequencing technique. Distamycin A enhanced bleomycin-catalyzed DNA cleavage in G.C rich sequences such as 5'-GGGGC-3' (under scoring; the cleaved nucleotide)....

journal_title：Biochemical and biophysical research communications

authors： Yamamoto K,Kawanishi S

更新日期：1992-02-28 00:00:00

abstract：:Enzymatic properties of phosphorylated urokinase plasminogen activator (P-uPA) (1) extracted from human carcinomatous cell line Detroit 562 cells were compared with those of non-phosphorylated uPA of urinary origin (nP-uPA). Using plasminogen as a substrate, the Km and Kcat of P-uPA were higher than that of nP-uPA whi...

journal_title：Biochemical and biophysical research communications

authors： Takahashi K,Kwaan HC,Koh E,Tanabe M

更新日期：1992-02-14 00:00:00

abstract：OBJECTIVE:Chondrocytes express many kinds of hormone receptors. The function of Follicle stimulating hormone (FSH) in the ovary is mediated by FSH receptor (FSHR). FSH receptor (FSHR) is found in many non-ovarian tissues, however it has been unclear if chondrocytes express FSHR. The purpose of this study is to determin...

journal_title：Biochemical and biophysical research communications

authors： Kong D,Guan Q,Li G,Xin W,Qi X,Guo Y,Zhao J,Xu J,Sun S,Gao L

更新日期：2018-01-01 00:00:00

abstract：:Crystallographic studies have shown independent binding sites for sugar and peptide ligands of concanavalin A, although they were considered functional mimics based on biochemical experiments. The topological correlation of 12-residue peptide with different carbohydrate ligands of concanavalin A showed similarity betw...

journal_title：Biochemical and biophysical research communications

authors： Jain D,Kaur KJ,Goel M,Salunke DM

更新日期：2000-06-16 00:00:00

abstract：:Ca2+/calmodulin-dependent protein kinases are implicated in regulating the Ca2+ signaling involved in T cell activation and in thymocyte selection. One of the earliest events in signaling through the T cell antigen receptor is activation of the protein tyrosine kinase p56lck. Following T cell activation or signaling t...

journal_title：Biochemical and biophysical research communications

authors： Bland MM,McDonald OB,Carrera AC

更新日期：1994-01-14 00:00:00

abstract：:Multidrug-resistant cells are characterized by the presence of P-glycoprotein on the plasma membrane, which binds and probably transports antitumor agents outside the cells. P-glycoprotein is also present in various normal tissues such as the adrenal gland. To investigate the physiological function of P-glycoprotein, ...

journal_title：Biochemical and biophysical research communications

authors： Naito M,Yusa K,Tsuruo T

更新日期：1989-02-15 00:00:00