当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > High definition profiling of autoantibodies to glutamic acid decarboxylases GAD65/GAD67 in stiff-person syndrome.

High definition profiling of autoantibodies to glutamic acid decarboxylases GAD65/GAD67 in stiff-person syndrome.

Abstract：

:Highly reliable biomarkers for the diagnosis of neurological diseases are not widely available. Here we evaluated a luciferase immunoprecipitation technology (LIPS) for the diagnosis of a CNS autoimmune disorder, stiff-person syndrome (SPS). Analysis by LIPS of 40 sera samples from SPS and control subjects for anti-GAD65 antibodies revealed dramatic titer differences allowing diagnosis of SPS with 100% sensitivity and 100% specificity. Anti-GAD65 antibody titers of SPS were segregated from controls with values greater than 23 standard deviations above the control subject mean. By analyzing patient antibody responses directly to GAD65 sub-fragments, the central region containing the decarboxylase catalytic domain was highly immunoreactive with all of the SPS sera, while the N- and C-terminal regions showed lower antibody titers and only reacted with subsets of SPS sera. Additional profiling revealed that some SPS patients showed autoantibodies against GAD67 and tyrosine hydroxylase, but no significant immunoreactivity was detected with cysteine sulfinic acid decarboxylase or GABA transaminase. This study validates LIPS as a robust method to interrogate autoantibodies for the diagnosis of SPS and potentially other neurological diseases.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Burbelo PD,Groot S,Dalakas MC,Iadarola MJ

doi

10.1016/j.bbrc.2007.11.077

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

1-7

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)02397-2

journal_volume

366

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Generation of highly site-specific DNA double-strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI.

    abstract：:We have determined the ability of two well-characterized eukaryotic homing endonucleases, I-PpoI from the myxomycete Physarum polycephalum and I-CreI from the green alga Chlamydomonas reinhardtii, to generate site-specific DNA double-strand breaks in human cells. These 18-kDa proteins cleave highly conserved 15- or 24...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0152

    authors： Monnat RJ Jr,Hackmann AF,Cantrell MA

    更新日期：1999-02-05 00:00:00

  • Inhibition of the calcium-activated chloride current in cardiac ventricular myocytes by N-(p-amylcinnamoyl)anthranilic acid (ACA).

    abstract：:N-(p-amylcinnamoyl)anthranilic acid (ACA), a phospholipase A(2) (PLA(2)) inhibitor, is structurally-related to non-steroidal anti-inflammatory drugs (NSAIDs) of the fenamate group and may also modulate various ion channels. We used the whole-cell, patch-clamp technique at room temperature to investigate the effects of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.069

    authors： Gwanyanya A,Macianskiene R,Bito V,Sipido KR,Vereecke J,Mubagwa K

    更新日期：2010-11-19 00:00:00

  • Synthesis of a nitric oxide-like factor from L-arginine by rat serosal mast cells: stimulation of guanylate cyclase and inhibition of platelet aggregation.

    abstract：:Rat serosal mast cells were tested for their ability to generate a nitric oxide-like factor by two bioassay systems: inhibition of platelet aggregation and stimulation of mast cell guanylate cyclase. Incubation of rat serosal mast cells with human washed platelets resulted in an inhibition of thrombin-induced platelet...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90372-t

    authors： Salvemini D,Masini E,Anggard E,Mannaioni PF,Vane J

    更新日期：1990-06-15 00:00:00

  • Expression of a functional IL-13Ralpha1 by rat B cells.

    abstract：:IL-13 mediates its effects through a complex receptor system including IL-4Ralpha and a functional IL-13Ralpha1. IL-13 has been reported to have no effects on mouse B cells due to a lack of receptor expression. However, on human B cells a functional IL-13Ralpha1 has been described. Here, we identified the rat IL-13Ral...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5682

    authors： Pierrot C,Beniguel L,Bègue A,Khalife J

    更新日期：2001-10-05 00:00:00

  • Increase in osmotolerance of Rhizobium fredii soybean isolate BD32 by the proB proA operon of Escherichia coli.

    abstract：:The proB proA operons (which are blocked by the feedback inhibition of proline production) of Escherichia coli wild type or with the mutation proBosm, blocking feedback inhibition effect of proline production, were cloned in a broad host range shuttle vector pVA 12-2. The hybrid plasmids pLVA(proB+A+) and pNSA(proBosm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2842

    authors： Neumivakin LV,Solovjev VP,Sokhansanj A,Tilba VA,Moseiko NA,Shachbasian RV,Piruzian ES

    更新日期：1995-12-26 00:00:00

  • argJ mutations are highly inducible by ethidium bromide in proB strains of Streptomyces lividans: implication of pathway interactions.

    abstract：:Spontaneous Arg- mutants arose at high frequencies in Streptomyces lividans. Exposure to ethidium bromide increased the frequency of arg instability. In Pro+ strains the induced arg mutants were mainly argG, but in the proB mutants, a new mutation, argJ, prevailed which lacked ornithine acetyltransferase activity and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92318-r

    authors： Chou CF,Chen CW

    更新日期：1992-12-15 00:00:00

  • Glycolysis and lipid synthesis in brown adipose tissue during ageing in the rat.

    abstract：:The maximal activity of the enzymes hexokinase and 6-phosphofructokinase were measured in interscapular brown adipose tissue during ageing in the rat. The activity of these glycolytic enzymes increased markedly during the suckling-weaning transition (20-22 days of age), but from 30 to 100 days of age the activities pe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91107-1

    authors： Simpson ML,Cooney GJ,Caterson ID,Newsholme EA

    更新日期：1986-10-15 00:00:00

  • Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts.

    abstract：:A monoclonal antibody, highly selective for the 5'-phosphopyridoxyl group, can be used to detect cytosolic pyridoxal-5'-phosphate binding proteins by an immunoblot procedure. This technique, when applied to sodium borohydride-treated cytosolic extracts obtained from normal rat liver at various stages of development as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91797-7

    authors： Kittler JM,Viceps-Madore D,Cidlowski JA,Thanassi JW

    更新日期：1983-04-15 00:00:00

  • Substrate specificity differences between recombinant rat testes endopeptidase EC 3.4.24.15 and the native brain enzyme.

    abstract：:We have characterized and compared the substrate specificity of affinity-purified recombinant rat testes endopeptidase EC 3.4.24.15 (EP 24.15) with that reported for the isolated brain enzyme. Of the peptides tested, only bradykinin, dynorphin A1-8, and neurotensin were efficiently cleaved by the recombinant enzyme (k...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1569

    authors： Lew RA,Hey NJ,Tetaz TJ,Glucksman MJ,Roberts JL,Smith AI

    更新日期：1995-04-26 00:00:00

  • Lipopolysaccharide prevents apoptosis induced by brefeldin A, an endoplasmic reticulum stress agent, in RAW 264.7 cells.

    abstract：:The effect of lipopolysaccharide (LPS) on the cell death induced by endoplasmic reticulum (ER) stress agents in RAW 264.7 cells was studied. LPS prevented the cell death by brefeldin A, but not thapsigargin and tunicamycin. CpG DNA as well as LPS prevented brefeldin A-induced cell death whereas tumor necrosis factor-a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.050

    authors： Islam S,Hassan F,Tumurkhuu G,Ito H,Koide N,Mori I,Yoshida T,Yokochi T

    更新日期：2006-02-10 00:00:00

  • The enhanced IL-18 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT).

    abstract：:Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and hu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02433-6

    authors： Cho D,Seung Kang J,Hoon Park J,Kim YI,Hahm E,Lee J,Yang Y,Jeon J,Song H,Park H,Kim T,Pang S,Kim CW,Il Hwang Y,Jae Lee W

    更新日期：2002-10-25 00:00:00

  • Salt-inducible kinase 2 regulates TFEB and is required for autophagic flux in adipocytes.

    abstract：:Dysregulation of autophagy has been observed in obesity and type 2 diabetes. Salt-inducible kinase 2 (SIK2), a member of the AMPK-related kinase family, is downregulated in adipocytes from obese or insulin resistant individuals and was previously demonstrated to regulate autophagy in cancer and normal cell lines. The ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.177

    authors： Negoita F,Säll J,Morén B,Stenkula K,Göransson O

    更新日期：2019-01-15 00:00:00

  • Transcriptional regulation of the human Liver X Receptor α gene by Hepatocyte Nuclear Factor 4α.

    abstract：:Liver X Receptors (LXRs) are sterol-activated transcription factors that play major roles in cellular cholesterol homeostasis, HDL biogenesis and reverse cholesterol transport. The aim of the present study was to investigate the mechanisms that control the expression of the human LXRα gene in hepatic cells. A series o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.031

    authors： Theofilatos D,Anestis A,Hashimoto K,Kardassis D

    更新日期：2016-01-15 00:00:00

  • Lipoic acid entrains the hepatic circadian clock and lipid metabolic proteins that have been desynchronized with advanced age.

    abstract：:It is well established that lipid metabolism is controlled, in part, by circadian clocks. However, circadian clocks lose temporal precision with age and correlates with elevated incidence in dyslipidemia and metabolic syndrome in older adults. Because our lab has shown that lipoic acid (LA) improves lipid homeostasis ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.05.112

    authors： Keith D,Finlay L,Butler J,Gómez L,Smith E,Moreau R,Hagen T

    更新日期：2014-07-18 00:00:00

  • Hepatocellular metabolism of 4-hydroxy-2,3-nonenal is impaired in conditions of chronic cholestasis.

    abstract：:4-Hydroxy-2,3-nonenal is a major aldehydic end-product of lipid peroxidation known to exert several biological and cytotoxic effects and to be produced during conditions of chronic cholestasis. Here we report that viable hepatocytes isolated from cholestatic livers of bile duct-ligated rats (BDL hepatocytes) show a si...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2338

    authors： Leonarduzzi G,Parola M,Muzio G,Garramone A,Maggiora M,Robino G,Poli G,Dianzani MU,Canuto RA

    更新日期：1995-09-14 00:00:00

  • Peroxynitrite reacts with biological nitrogen-containing cyclic molecules by a radical pathway, as demonstrated by ultraweak luminescence coupled with ESR technique.

    abstract：:Ultraweak luminescence (uwCL) was coupled with electron spin resonance to study the reactions of 3 heterocyclic compounds (tryptophan, serotonin and imidazole) with peroxynitrite at pH 8.7. Tryptophan and serotonin reacted with emission of a flash peak of light (5 s) followed by a long-living light emission of +/- 80 ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0805

    authors： Mouithys-Mickalad A,Kohnen S,Deby C,Noels AF,Lamy M,Deby-Dupont G

    更新日期：1999-06-07 00:00:00

  • The association of renin-angiotensin system genes with the progression of hepatocellular carcinoma.

    abstract：:Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Angiogenesis is reported to play a pivotal role in the occurrence, development and metastasis of HCC. The renin-angiotensin system (RAS) is involved in the regulation of angiogenesis. Here, based on the analysis of HCC datasets from Gene Expre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.02.030

    authors： Ye G,Qin Y,Lu X,Xu X,Xu S,Wu C,Wang X,Wang S,Pan D

    更新日期：2015-03-27 00:00:00

  • Gene expression of VIP receptor in rat lymphocytes.

    abstract：:In the present report we analyze by reverse transcription (RT) and polymerase chain reaction (PCR) the expression of vasoactive intestinal peptide receptors (VIP-R) in rat thymocytes and peripheral T and B lymphocytes. We have obtained a specific VIP-R cDNA product of 311 bp identical in size to that obtained from lun...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2369

    authors： Gomariz RP,Garrido E,Leceta J,Martinez C,Abalo R,Delgado M

    更新日期：1994-09-30 00:00:00

  • Drosophila CK2 phosphorylates Hairy and regulates its activity in vivo.

    abstract：:Hairy is a repressor that regulates bristle patterning, and its loss elicits ectopic bristles (neural hyperplasia). However, it has remained unknown whether Hairy is regulated by phosphorylation. We describe here the interaction of protein kinase CK2 and Hairy. Hairy is robustly phosphorylated by the CK2-holoenzyme (C...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.084

    authors： Kahali B,Trott R,Paroush Z,Allada R,Bishop CP,Bidwai AP

    更新日期：2008-09-05 00:00:00

  • Bovine liver cDNA clones encoding a precursor of the alpha-subunit of the mitochondrial ATP synthase complex.

    abstract：:cDNA clones encoding a precursor of the alpha-subunit of the mitochondrial ATP synthase complex have been isolated from a bovine liver cDNA library using the alpha-subunit gene from Saccharomyces cerevisiae as a probe. Analyses of the nucleotide sequence of these cDNA clones reveal that the bovine liver ATP synthase a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80709-5

    authors： Breen GA

    更新日期：1988-04-15 00:00:00

  • Activation of mutant protein kinase Cgamma leads to aberrant sequestration and impairment of its cellular function.

    abstract：:Mutations in protein kinase Cgamma (PKCgamma) cause the neurodegenerative disease spinocerebellar ataxia type 14 (SCA14). In this study, expression of an extensive panel of known SCA14-associated PKCgamma mutations as fusion proteins in cell culture led to the consistent formation of cytoplasmic aggregates in response...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.05.072

    authors： Doran G,Davies KE,Talbot K

    更新日期：2008-08-01 00:00:00

  • Monte Carlo studies of lipid chains and gramicidin A in a model membrane.

    abstract：:The Monte Carlo method has been used to simulate the equilibrium properties of a planar array of 94 saturated lipid chains and one monomer of Gramicidin A. Chains are free to move laterally in the layer plane and to change conformation via gauche rotations and long axis rotations in a continuum. All non-hydrogen atoms...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91025-5

    authors： Xing J,Scott HL

    更新日期：1989-11-30 00:00:00

  • Gene structure of heat shock proteins 61KDa and 12KDa (thermophilic chaperonins) of thermophilic bacterium PS3.

    abstract：:Heat shock proteins 60 (hsp60) and 10 (hsp10) are essential for the formation and restoration of many supramolecular structures. For reconstitution of these structures, we isolated stable hsps of 61kDa and 12kDa, which are similar to hsp60 and hsp10, respectively, from the supernatant fraction of thermophilic bacteriu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91408-5

    authors： Tamada H,Ohta T,Hamamoto T,Otawara-Hamamoto Y,Yanagi M,Hiraiwa H,Hirata H,Kagawa Y

    更新日期：1991-08-30 00:00:00

  • Neuropeptide Y potentiates the luteinizing hormone (LH) response to LH-releasing hormone in men.

    abstract：:It has been demonstrated in several animal species that neuropeptide Y (NPY) exerts a modulatory effect on luteinizing hormone (LH) secretion. However, whether NPY plays a similar role also in humans has yet to be determined. Therefore, in this study we examined the effect of human NPY on the anterior pituitary hormon...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1565

    authors： Watanobe H,Nigawara T,Anzai J,Sakihara S,Kageyama K,Nasushita R,Sasaki S,Habu S,Takebe K

    更新日期：1994-04-29 00:00:00

  • The importance of the hydroxyl moieties for inhibition of the Ca(2+)-ATPase by trilobolide and 2,5-di(tert-butyl)-1,4-benzohydroquinone.

    abstract：:Trilobolide and 2,5-di(tert-butyl)-1,4-benzohydroquinone (BHQ) are potent inhibitors of the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum. Desoxytrilobolide and 2,5-di(tert-butyl)-1,4-diacetylphenol (acetyl-BHQ) have much lower potencies than their parent compounds and 2,5-di(tert-butyl)-1,4-benzoquinone (BQ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1316

    authors： Wictome M,Holub M,East JM,Lee AG

    更新日期：1994-03-15 00:00:00

  • Biotransformation of 1-nitropyrene and 2-nitrofluorene to novel metabolites, the corresponding formylamino compounds, in animal bodies.

    abstract：:The present study provides the first evidence that nitropolycyclic aromatic hydrocarbons such as 1-nitropyrene and 2-nitrofluorene are metabolized to the corresponding formylamino compounds in animal bodies. The study shows that the formation of such novel metabolites, 1-formylaminopyrene and 2-formylaminofluorene, is...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90284-1

    authors： Tatsumi K,Amano H

    更新日期：1987-01-30 00:00:00

  • Involvement of superoxide radical in signal transduction regulating stomatal movements.

    abstract：:Stomatal movements are regulated by alternate phase transition in the lipophilic domains of membrane initiated by blue light through activation of plasmalemma redox system. ABA blocks the blue light activated redox reactions leading to the accumulation of superoxide (O2.-) radical inducing melting of the membrane and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2625

    authors： Purohit S,Kumar GP,Laloraya M,Laloraya MM

    更新日期：1994-11-30 00:00:00

  • In vitro transformation of mesenchymal stem cells by oncogenic H-rasVal12.

    abstract：:Tissue stem cells may serve as progenitors for malignant tumors derived from the same tissue. Here, we report the establishment of immortalized human mesenchymal stem cells (ihMSC) and tested the feasibility of using ihMSC as presarcomatous cells. Immortalization was achieved by introducing the genes for human telomer...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.137

    authors： Shima Y,Okamoto T,Aoyama T,Yasura K,Ishibe T,Nishijo K,Shibata KR,Kohno Y,Fukiage K,Otsuka S,Uejima D,Nakayama T,Nakamura T,Kiyono T,Toguchida J

    更新日期：2007-02-02 00:00:00

  • Synergistic potentiation of thiazolidinedione-induced ST 13 preadipocyte differentiation by RAR synergists.

    abstract：:Peroxisome proliferator-activated receptor gamma (PPAR gamma) belongs to a nuclear receptor super family that functions as a master regulator of adipocyte differentiation. PPAR gamma binds its DNA response element together with a partner, retinoid X receptor (RXR), in fat cells. Five RXR ligands (HX600, HX630, DA022, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4172

    authors： Sato M,Yajima Y,Kawashima S,Tanaka K,Kagechika H

    更新日期：2001-01-26 00:00:00

  • KMT Set7/9 is a new regulator of Sam68 STAR-protein.

    abstract：:Lysine-specific methyltransferase Set7/9 (KMT7) belongs to the SET domain family of proteins. Besides the SET domain, Set7/9 also contains a so-called MORN (Membrane Occupation and Recognition Nexus) domain whose function in high eukaryotes is largely unknown. Set7/9 has been shown to specifically methylate both histo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.03.017

    authors： Vasileva E,Shuvalov O,Petukhov A,Fedorova O,Daks A,Nader R,Barlev N

    更新日期：2020-05-14 00:00:00