Heat stability of the potato tuber ADP-glucose pyrophosphorylase: role of Cys residue 12 in the small subunit.

Abstract:

:Most of the ADP-glucose pyrophosphorylases from different sources are stable to a heat treatment. We found that in the potato (Solanum tuberosum L.) tuber enzyme, the intermolecular disulfide bridge located between Cys12 of the small subunits is responsible for the stability at 60 degrees C. When this unique disulfide bond is cleaved the enzyme is stable up to 40 degrees C. Mutation of Cys12 in the small subunit into either Ala or Ser yielded enzymes with stability similar to the reduced form of the wild type. Concurrently, the enzyme with a truncated small subunit on the N-terminal was stable only up to 40 degrees C. Thus, the N-terminal is important for the stability of the enzyme because of the presence of a disulfide bond.

authors

Ballicora MA,Fu Y,Frueauf JB,Preiss J

doi

10.1006/bbrc.1999.0469

subject

Has Abstract

pub_date

1999-04-21 00:00:00

pages

782-6

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(99)90469-2

journal_volume

257

pub_type

杂志文章
  • Immunological and conformation characterization of a phosphorylated immunodominant epitope on the paired helical filaments found in Alzheimer's disease.

    abstract::The immunological recognition pattern of one of the most commonly used monoclonal antibodies, PHF-1, which detects the paired helical filaments of Alzheimer's disease, exhibits a high degree of similarity with the recognition of a polyclonal antibody, anti-T3P, raised against a synthetic phosphopeptide, GAEIVYKS(Phosp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(92)91264-q

    authors: Lang E,Szendrei GI,Lee VM,Otvos L Jr

    更新日期:1992-09-16 00:00:00

  • Presence of Ca(2+)-sensitive and -insensitive SM22 alpha isoproteins in bovine aorta.

    abstract::Two proteins (25- and 22-kDa) that cross-react with anti-gizzard SM22 alpha antibody were isolated from bovine aorta. The former, identical to a SM22 alpha homolog reported previously (Kobayashi, R., Kubota, T., and Hidaka, H. (1994) Biochem. Biophys. Res. Commun. 198, 1275-1280), associates with the membrane fraction...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.1784

    authors: Shishibori T,Yamashita K,Bandoh J,Oyama Y,Kobayashi R

    更新日期:1996-12-04 00:00:00

  • BDNF is induced by wild-type alpha-synuclein but not by the two mutants, A30P or A53T, in glioma cell line.

    abstract::Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases but its etiology is unclear. Alpha-synuclein (alpha-SN) is a major component of Lewy bodies and Lewy neurites, and its missense mutations, A30P and A53T, cause familial PD. In PD, alpha-SN-positive glial inclusions are distributed mainly ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.04.012

    authors: Kohno R,Sawada H,Kawamoto Y,Uemura K,Shibasaki H,Shimohama S

    更新日期:2004-05-21 00:00:00

  • Interaction of cavin-1/PTRF leucine zipper domain 2 and its congenital generalized lipodystrophy mutant with model membranes.

    abstract::The organization of caveolae ultrastructures in the plasma membrane and the functions they dictate are mediated by membrane-embedded caveolins (caveolin-1, 2, 3) and peripherally attached cavins (cavin-1, 2, 3, 4). Mutations in caveolin and cavin genes are associated with a variety of human diseases. Cavin-1/PTRF muta...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.10.167

    authors: Mohapatra A,Lokappa SB,Chaudhary N

    更新日期:2020-01-15 00:00:00

  • Diacylglycerol causes major structural transitions in phospholipid bilayer membranes.

    abstract::We demonstrate for the first time that major structural changes are imposed on various phospholipid bilayers by diacylglycerol, a product of phosphatidylinositol metabolism. By 5 mole percent in phosphatidylethanolamine a lamellar to hexagonal transition starts that is complete at 10 mole percent. At 30 mole percent i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(84)91580-8

    authors: Das S,Rand RP

    更新日期:1984-10-30 00:00:00

  • A novel insertion of a rearranged L1 element in exon 44 of the dystrophin gene: further evidence for possible bias in retroposon integration.

    abstract::L1 elements are mammalian retrotransposons contributing to genome evolution and causing rare mutations in human. We describe a de novo insertion of an L1 element into the dystrophin gene resulting in skipping of exon 44 and causing Duchenne muscular dystrophy in a boy. The L1 element was rearranged due to the twin-pri...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.06.071

    authors: Musova Z,Hedvicakova P,Mohrmann M,Tesarova M,Krepelova A,Zeman J,Sedlacek Z

    更新日期:2006-08-18 00:00:00

  • Expression of a novel cardiac-specific tropomyosin isoform in humans.

    abstract::Tropomyosins are a family of actin binding proteins encoded by a group of highly conserved genes. Humans have four tropomyosin-encoding genes: TPM1, TPM2, TPM3, and TPM4, each of which is known to generate multiple isoforms by alternative splicing, promoters, and 3' end processing. TPM1 is the most versatile and encod...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.06.084

    authors: Denz CR,Narshi A,Zajdel RW,Dube DK

    更新日期:2004-08-06 00:00:00

  • Identification and characterization of signal peptide of Mitofusin1 (Mfn1).

    abstract::Mitofusin1 (Mfn1) mediates outer mitochondrial membrane (OMM) fusion in Opisthokonts. The uncharacterized TM comprises to two helices (namely, the TM1 and TM2) connected by an intermembrane loop. Consistent with previous studies, our results from in silico analyses show that all mitofusins lack N terminal-MTS and the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.12.165

    authors: Sinha S,Aradhyam GK

    更新日期:2019-02-12 00:00:00

  • Nrf2 regulates cellular behaviors and Notch signaling in oral squamous cell carcinoma cells.

    abstract::Oxidative stress is known to play a pivotal role in the development of oral squamous cell carcinoma (OSCC). We have demonstrated that activation of the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway has chemopreventive effects against oxidative stress-associated OSCC. However, Nrf2 have dual role...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.08.049

    authors: Fan H,Paiboonrungruan C,Zhang X,Prigge JR,Schmidt EE,Sun Z,Chen X

    更新日期:2017-11-04 00:00:00

  • Inorganic phosphate regulates CryIVA protoxin expression in Bacillus thuringiensis israelensis.

    abstract::The role of nutritional factors during CryIVA protoxin expression in Bacillus thuringiensis israelensis (Bti) has been investigated. Inorganic phosphate (Pi) was found to stimulate 135 kD protoxin synthesis by Bti cells. There was a corresponding increase in the cryIVA specific mRNA in the presence of Pi. Inorganic ph...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1999.1094

    authors: Banerjee-Bhatnagar N

    更新日期:1999-08-27 00:00:00

  • Lipid peroxidation as a possible cause of TCDD toxicity.

    abstract::The target tissues of TCDD, the dysfunctions that result in death in experimental animals, and the ultimate biochemical lesion(s) caused by TCDD are not known despite numerous studies. We have shown by the thiobarbituric acid and conjugated diene methods that TCDD induces hepatic lipid peroxidation in rats. The lipid ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91377-3

    authors: Stohs SJ,Hassan MQ,Murray WJ

    更新日期:1983-03-29 00:00:00

  • Wnt-independent activation of beta-catenin mediated by a Dkk1-Fz5 fusion protein.

    abstract::An XWnt8-Fz5 fusion protein synergizes with LRP6 to potently activate beta-catenin-dependent signaling. Here, we generated a fusion in which XWnt8 was fused to the N-terminus of LRP6 and show it synergizes with both Fz4 and Fz5 to potently transactivate beta-catenin-dependent Wnt signaling. Based on this, we hypothesi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.01.009

    authors: Holmen SL,Robertson SA,Zylstra CR,Williams BO

    更新日期:2005-03-11 00:00:00

  • Expression analysis for inverted effects of serotonin transporter inactivation.

    abstract::Inactivation of serotonin transporter (HTT) by pharmacologically in the neonate or genetically increases risk for depression in adulthood, whereas pharmacological inhibition of HTT ameliorates symptoms in depressed patients. The differing role of HTT function during early development and in adult brain plasticity in c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.01.041

    authors: Ichikawa M,Okamura-Oho Y,Shimokawa K,Kondo S,Nakamura S,Yokota H,Himeno R,Lesch KP,Hayashizaki Y

    更新日期:2008-03-28 00:00:00

  • A knock-in mouse model of N-terminal R420W mutation of cardiac ryanodine receptor exhibits arrhythmogenesis with abnormal calcium dynamics in cardiomyocytes.

    abstract::Cardiac ryanodine receptor gene (RyR2) mutations cause fatal arrhythmogenic diseases such as catecholaminergic polymorphic ventricular tachycardia and arrhythmogenic right ventricular cardiomyopathy. The N-terminal region of RyR2 is one of the hot spots for mutations. In this study, we investigated cardiac phenotypes ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.08.132

    authors: Okudaira N,Kuwahara M,Hirata Y,Oku Y,Nishio H

    更新日期:2014-09-26 00:00:00

  • Expression of soluble active human beta 1,4 galactosyltransferase in Saccharomyces cerevisiae.

    abstract::Sequences coding for the cytoplasmic and transmembrane domains were removed from the cDNA of the human Golgi resident membrane protein beta 1,4 galactosyltransferase (gal-T). The remaining sequences coding for the stem and catalytical domains of this glycosyltransferase were fused to sequences coding for the yeast inv...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.1683

    authors: Kleene R,Krezdorn CH,Watzele G,Meyhack B,Herrmann GF,Wandrey C,Berger EG

    更新日期:1994-05-30 00:00:00

  • A specific, photolabile and irreversible antagonist (L662,025) of the PAF-receptor.

    abstract::PAF (0.2 microM) induced maximal platelet aggregation in human PRP and [3H]-PAF (1-5 nM) binding to platelet membrane preparations had Kd value of 3.8 nM and Bmax of 200 fmoles/mg of protein. Without UV irradiation, a synthetic azido tetrahydrofuran derivative L662,025 was a reversible and competitive PAF-receptor ant...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)91554-4

    authors: Hussaini IM,Shen TY

    更新日期:1989-05-30 00:00:00

  • SSA/Ro52 autoantigen interacts with Dcp2 to enhance its decapping activity.

    abstract::We identified human decapping enzyme 2 (hDCP2) as a binding protein with Ro52, being colocalized in processing bodies (p-bodies). We also showed that the N-terminus and C-terminus of Ro52 bound to hDCP2. Moreover, Ro52 enhanced decapping activity of hDCP2 in a dose-dependent manner. Our data support the novel notion o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2008.03.075

    authors: Yamochi T,Ohnuma K,Hosono O,Tanaka H,Kanai Y,Morimoto C

    更新日期:2008-05-23 00:00:00

  • Identification of activators of methionine sulfoxide reductases A and B.

    abstract::The methionine sulfoxide reductase (Msr) family of enzymes has been shown to protect cells against oxidative damage. The two major Msr enzymes, MsrA and MsrB, can repair oxidative damage to proteins due to reactive oxygen species, by reducing the methionine sulfoxide in proteins back to methionine. A role of MsrA in a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2015.12.077

    authors: Cudic P,Joshi N,Sagher D,Williams BT,Stawikowski MJ,Weissbach H

    更新日期:2016-01-22 00:00:00

  • Isolation of wound healing/regeneration genes using restrictive fragment differential display-PCR in MRL/MPJ and C57BL/6 mice.

    abstract::Wound healing in mammals can take several weeks to months and the process is always accompanied by scar formation. Wound healing mechanisms that mimic regeneration are not found in most mature mammalian tissues. However, the MRL/MPJ (MRL) mouse has the unique capacity to regenerate ear hole wound completely in less th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.02.143

    authors: Masinde G,Li X,Baylink DJ,Nguyen B,Mohan S

    更新日期:2005-04-29 00:00:00

  • A cold-regulated Arabidopsis gene encodes a polypeptide having potent cryoprotective activity.

    abstract::We previously showed that cor15, a cold-regulated gene from Arabidopsis thaliana, encodes a 15 kDa polypeptide designated COR15. Here we report that COR15 has potent cryoprotective activity in an in vitro cryoprotection assay. Specifically, COR15 was very effective in protecting the cold-labile enzyme lactate dehydrog...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(05)80304-3

    authors: Lin C,Thomashow MF

    更新日期:1992-03-31 00:00:00

  • Urocortin 2 induces tyrosine hydroxylase phosphorylation in PC12 cells.

    abstract::Urocotins (Ucns) are newly discovered members of the corticotropin-releasing factor (CRF) neuropeptide family. Ucn 2 is expressed in the adrenal medulla, and its receptor, CRF2 receptor, is also expressed in the adrenal gland. To predict the physiological significance of Ucn 2 expression in the adrenal medulla, we exa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2005.03.031

    authors: Nemoto T,Mano-Otagiri A,Shibasaki T

    更新日期:2005-05-13 00:00:00

  • Dexamethasone increases neutral sphingomyelinase activity and sphingosine levels in 3T3-L1 fibroblasts.

    abstract::The activity of neutral sphingomyelinase (EC 3.1.4.12) in a plasma membrane enriched fraction was found to be increased in dexamethasone treated cells. The elevation of sphingomyelinase activity was blocked by cycloheximide indicating that protein synthesis was required for the steroid action. Ceramidase (EC3.5.1.23) ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)92068-b

    authors: Ramachandran CK,Murray DK,Nelson DH

    更新日期:1990-03-16 00:00:00

  • Molecular characterization of human and bovine ceruloplasmin using MALDI-TOF mass spectrometry.

    abstract::Using SDS-PAGE and MALDI-TOF mass spectrometry, we investigated the difference in the molecular structure between human and bovine ceruloplasmin. In both cases, we found that the protein is present in two majors forms of different molecular mass. The difference between human and bovine ceruloplasmin was more obvious w...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2001.5881

    authors: Boivin S,Aouffen M,Fournier A,Mateescu MA

    更新日期:2001-11-09 00:00:00

  • Two phosphorylation-independent sites on the p85 SH2 domains bind A-Raf kinase.

    abstract::Src homology 2 (SH2) domains mediate phosphotyrosine (pY)-dependent protein:protein interactions involved in signal transduction pathways. We have found that the SH2 domains of the 85-kDa alpha subunit (p85) of phosphatidylinositol 3-kinase (PI3 kinase) bind directly to the serine/threonine kinase A-Raf. In this repor...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2002.6347

    authors: Fang Y,Johnson LM,Mahon ES,Anderson DH

    更新日期:2002-02-01 00:00:00

  • An aortic spontaneously active phosphatase dephosphorylates myosin and inhibits actin-myosin interaction.

    abstract::Actin-myosin interaction in aortic actomyosin reportedly requires phosphorylation of the 20,000 dalton myosin light chains. A spontaneously active phosphatase which dephosphorylates phosphorylase a and isolated phosphorylated cardiac myosin light chains was extracted from bovine aortic smooth muscle. This enzyme, when...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91385-2

    authors: Di Salvo J,Gifford D,Bialojan C,Rüegg JC

    更新日期:1983-03-29 00:00:00

  • The same nuclear proteins bind the proximal CACCC box of the human beta-globin promoter and a similar sequence in the enhancer.

    abstract::Using in vitro assays, we show that nuclear proteins related to the Sp1 and GT-1 factors bind to a CACCC box sequence in the human beta-globin enhancer, adjacent to binding sites for the erythroid-specific factor NFE1 and the ubiquitous factor CP1. The same proteins are known to bind to the proximal, but not to the di...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)91695-1

    authors: Giglioni B,Comi P,Ronchi A,Mantovani R,Ottolenghi S

    更新日期:1989-10-16 00:00:00

  • Transient expression of multiple genes in salinity-stressed young seedlings of rice (Oryza sativa L.) cv. bura rata.

    abstract::To date only about 15 abundantly expressed osmoresponsive genes/proteins have been described in rice cultivars. Using in vivo radiolabeling followed by two-dimensional electrophoresis and autoradiography, a record number of at least 35 salinity stress-induced polypeptides (14-90 kDa) and 17 salt stress-repressed polyp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6521

    authors: Ramani S,Apte SK

    更新日期:1997-04-28 00:00:00

  • The human tumour suppressor LATS1 is activated by human MOB1 at the membrane.

    abstract::Downregulation of the LATS1 tumour suppressor protein kinase contributes to tumour formation in mammals and flies. Strikingly, the tumour suppressor activity depends on the interaction with Dmob (Drosphila Mps1-One binder) in Drosophila melanogaster. Recently, human LATS1 was reported to interact with human MOB1 (hMOB...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.03.244

    authors: Hergovich A,Schmitz D,Hemmings BA

    更新日期:2006-06-23 00:00:00

  • Association of FMRP with ribosomal precursor particles in the nucleolus.

    abstract::The fragile X syndrome, one of the most common forms of inherited mental retardation, is caused by an expansion of a polymorphic CGG repeat upstream the coding region of the FMR1 gene. These expansions are associated with hypermethylation of the FMR1 gene, which results in the absence of the gene product, the FMR1 pro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1996.1126

    authors: Willemsen R,Bontekoe C,Tamanini F,Galjaard H,Hoogeveen A,Oostra B

    更新日期:1996-08-05 00:00:00

  • Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function.

    abstract::Malignant human glioma D-298 MG amplifies a rearranged epidermal growth factor receptor (EGFR) gene (c-erbB proto-oncogene), resulting in an in-frame deletion of 83 amino acids in domain IV of the extracellular domain of the EGFR. EGF and transforming growth factor-a (TGF-a) bound to the mutant EGFR with high affinity...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)91051-d

    authors: Humphrey PA,Gangarosa LM,Wong AJ,Archer GE,Lund-Johansen M,Bjerkvig R,Laerum OD,Friedman HS,Bigner DD

    更新日期:1991-08-15 00:00:00