Thylakoid-bound chloroplast DNA from spinach is enriched for replication forks.

Abstract:

:Chloroplast DNA is bound to the thylakoids of spinach chloroplasts. To examine a possible role for thylakoid-bound DNA in chloroplast DNA replication, vesicles formed by treating chloroplasts in 3.5 mM MgCl2 were used. Chloroplast DNA fragments are bound to the surface of these vesicles. Chloroplast DNA isolated from vesicles that had been first treated with Eco R1 contained 10% of branched fragments whereas chloroplast DNA isolated from intact chloroplasts and treated with Eco R1 contained 2% of branched fragments. This result is consistent with the growing replication fork of chloroplast DNA being associated with the chloroplast internal membrane system. Branched fragments from the chloroplast DNA digested with Eco R1 prior to the isolation from the vesicle contained fragments of unequal length. Membrane binding in chloroplasts may have a similar role in DNA replication as it does in bacteria.

authors

Lindbeck AG,Rose RJ

doi

10.1016/s0006-291x(05)80194-9

subject

Has Abstract

pub_date

1990-10-15 00:00:00

pages

204-10

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)80194-9

journal_volume

172

pub_type

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