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Isolation and initial characterization of a Bacillus subtilis mutant with novel protease secretion capability.

Abstract：

:A bank of pTV32 (Tn 917 lacZ) - generated Bacillus subtilis mutants were examined on milk agar for the ability to produce proteases at 48 degrees C. A single mutant, BUL786, was isolated, which could hydrolyze casein after overnight incubation at 48 degrees C. This mutant secreted protease 10 fold more at 48 degrees C when compared to 37 degrees C, and part of the activity appears to be 48 degrees C-specific. At high temperatures, other strains of B. subtilis, including hyperprotease secretors, were unable to secrete protease to any significant degree. The BUL786 strain is missing the 97K major heat shock protein. Since a number of other proteins also appear to be secreted at 48 degrees C, this mutant may be a hypersecretor of exported proteins at temperatures greater than 45 degrees C.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Qoronfleh MW,Streips UN

doi

10.1016/s0006-291x(86)80528-9

subject

Has Abstract

pub_date

1986-07-31 00:00:00

pages

526-32

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(86)80528-9

journal_volume

138

pub_type

杂志文章

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