Abstract:
:Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin. We found that Fos-choline and CHAPS detergents counteracted each other's inhibitory effects on cell-free translation activity and thereby allowed the efficient production and subsequent purification of functional bacteriorhodopsin in high yield.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Genji T,Nozawa A,Tozawa Ydoi
10.1016/j.bbrc.2010.08.119subject
Has Abstractpub_date
2010-10-01 00:00:00pages
638-42issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(10)01640-2journal_volume
400pub_type
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