Overexpression of a phosphate transporter gene ZmPt9 from maize influences growth of transgenic Arabidopsis thaliana.

Abstract:

:Phosphate transporters (PHTs) are well-known for their roles in phosphate uptake in plants. However, their actions in imparting plant growth in plants are still not so clear. In our previous study, we observed that maize PHT1 gene ZmPt9 plays a significant role in phosphate uptake. In this study, we further characterized ZmPt9 in response to low phosphate condition through ZmPt9 promoter inductive analysis by GUS staining and quantification. To elucidate the function of ZmPt9, we generated overexpression plant in Arabidopsis. ZmPt9 overexpressing Arabidopsis plants conferred small leaves and early flowering compared with the wild-type plants. In addition, ZmPt9 can complement the late flowering phenotype of Arabidopsis mutant pht1;2. The qRT-PCR analysis revealed that overexpression of ZmPt9 in Arabidopsis changed expression levels of some flowering-related genes. Further expressed detection of hormone related genes revealed that GA and auxin maybe the main determinant for growth influences of ZmPt9. In conclusion, these results suggest that apart from phosphate transport activity, ZmPt9 can be further exploited for improving crops growth.

authors

Xu Y,Bao H,Fei H,Zhou W,Li X,Liu F

doi

10.1016/j.bbrc.2020.09.039

subject

Has Abstract

pub_date

2020-09-19 00:00:00

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(20)31780-0

pub_type

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