Abstract:
:Protein Z was purified from rabbit liver cytosol by affinity chromatography on oleic acid-agarose and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After removal of sodium dodecyl sulfate, the renatured protein was found to bind heme and bilirubin with a Kd of approximately 1 microM which produced large red shifts in their absorption spectra. On isoelectric focusing, rabbit protein Z exhibited two main bands with pI around 6.0.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Vincent SH,Holeman B,Muller-Eberhard Udoi
10.1016/0006-291x(85)91172-6subject
Has Abstractpub_date
1985-10-30 00:00:00pages
575-81issue
2eissn
0006-291Xissn
1090-2104pii
0006-291X(85)91172-6journal_volume
132pub_type
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