Purification and characterization of protein Z from rabbit liver cytosol.

Abstract:

:Protein Z was purified from rabbit liver cytosol by affinity chromatography on oleic acid-agarose and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After removal of sodium dodecyl sulfate, the renatured protein was found to bind heme and bilirubin with a Kd of approximately 1 microM which produced large red shifts in their absorption spectra. On isoelectric focusing, rabbit protein Z exhibited two main bands with pI around 6.0.

authors

Vincent SH,Holeman B,Muller-Eberhard U

doi

10.1016/0006-291x(85)91172-6

subject

Has Abstract

pub_date

1985-10-30 00:00:00

pages

575-81

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(85)91172-6

journal_volume

132

pub_type

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