Analysis of cag-8: a novel poly(Q)-encoding gene in the mouse brain.

abstract：:The microtubule motor kinesin-1 is responsible for the nuclear positioning during myogenesis. Here we show that the coiled-coil stalk/tail domain containing the kinesin light chain (KLC) binding sites targets to the perinuclear region like endogenous Kif5b, while the globular tail domain cannot. To investigate which f...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Xue W,Li X,Lin R,Cui J,Huang JD

更新日期：2013-03-08 00:00:00

abstract：:The Frizzled-type cysteine-rich domain (CRD) is a binding motif for soluble-type glycoprotein WNTs, which play key roles in embryogenesis and carcinogenesis. Here, we have cloned and characterized a novel gene MFRP, encoding a type II transmembrane protein with CRD. In addition to CRD, two tandem-repeats containing th...

journal_title：Biochemical and biophysical research communications

authors： Katoh M

更新日期：2001-03-23 00:00:00

abstract：:The activation of chloroplast fructose-1,6-bisphosphatase by fructose-1,6-bisphosphate, Ca2+, DTT and chloroplast thioredoxin-f is prevented by either spermidine or spermine; on the contrary, other amino compounds do not replace polyamines in this reversible effect. On the other hand, neither spermidine nor spermine m...

journal_title：Biochemical and biophysical research communications

authors： Corley E,Wolosiuk RA,Hertig CM

更新日期：1983-09-15 00:00:00

abstract：:Our previously synthesized 37 compounds, which are 2,5,6-substituted benzoxazole, benzimidazole, benzothiazole, and oxazolo(4,5-b)pyridine derivatives, were tested for their eukaryotic DNA topoisomerase II inhibitory activity in cell free system and 28 were found to inhibit the topoisomerase II at an initial concentra...

journal_title：Biochemical and biophysical research communications

authors： Pinar A,Yurdakul P,Yildiz I,Temiz-Arpaci O,Acan NL,Aki-Sener E,Yalcin I

更新日期：2004-04-30 00:00:00

abstract：:DNA topoisomerase type I and II activities were determined by serial dilution in nuclear extracts from control and ataxia-telangiectasia lymphoblastoid cells. Topoisomerase I activity, assayed by relaxation of supercoiled plasmid DNA, was found to be approximately the same in both cell types. In order to remove interf...

journal_title：Biochemical and biophysical research communications

authors： Mohamed R,Pal Singh S,Kumar S,Lavin MF

更新日期：1987-11-30 00:00:00

abstract：:The protein kinase CK2 holoenzyme is composed of two regulatory beta- and two catalytic alpha- or alpha(')-subunits. There is ample evidence for the binding of individual subunits of CK2 to various cellular proteins and, moreover, for functions of the individual subunits, which are different from their roles in the ho...

journal_title：Biochemical and biophysical research communications

authors： Faust M,Kartarius S,Schwindling SL,Montenarh M

更新日期：2002-08-09 00:00:00

abstract：:Human glutathione synthetase is responsible for catalyzing the final step in glutathione biosynthesis. It is a homodimer with a monomer subunit MW of 52 kDa. Kinetic analysis reveals a departure from linearity of the Lineweaver-Burk double reciprocal plot for the binding of gamma-glutamyl substrate, indicating coopera...

journal_title：Biochemical and biophysical research communications

authors： Njalsson R,Norgren S,Larsson A,Huang CS,Anderson ME,Luo JL

更新日期：2001-11-23 00:00:00

abstract：:The yeast two-hybrid system was used to screen for proteins that interact in vivo with Saccharomyces cerevisiae Rpg1p/Tif32p, the large subunit of the translation initiation factor 3 core complex (eIF3). Eight positive clones encoding portions of the SLA2/END4/MOP2 gene were isolated. They overlapped in the region of ...

journal_title：Biochemical and biophysical research communications

authors： Palecek J,Hasek J,Ruis H

更新日期：2001-04-20 00:00:00

abstract：:Ribosome-inactivating proteins (RIPs) are N-glycosidases (EC3.2.2.22) that universally inactivate the ribosome, thereby inhibiting protein biosynthesis. In this study, a novel type I RIPs named SEBIN was identified in Sapium sebiferum. Nuclear acid depurine experiment showed that SEBIN had rRNA N-Glycosidase activity....

journal_title：Biochemical and biophysical research communications

authors： Wu Y,Mao Y,Jin S,Hou J,Du H,Yang M,Wu L

更新日期：2015-08-07 00:00:00

abstract：:We examined the mechanism of arachidonate release induced by somatostatin-14 (SS14) in CHO-K1 cells overexpressing rat hippocampal somatostatin receptor SSTR4. SSTR4 couples to pertussis toxin (PTX)-sensitive G-protein in CHO cells and does not lead to phosphoinositides breakdown or intracellular calcium ([Ca2+]i) mob...

journal_title：Biochemical and biophysical research communications

authors： Sakanaka C,Ferby I,Waga I,Bito H,Shimizu T

更新日期：1994-11-30 00:00:00

abstract：:A cDNA for the rat bone morphogenetic protein (BMP) type IA receptor (BMPR-IA) was isolated from a dental pulp cell cDNA library. The rat BMPR-IA cDNA encodes a protein of 532 amino acids with a single transmembrane domain and a putative serine/threonine kinase domain. The overall amino acid sequence identity between ...

journal_title：Biochemical and biophysical research communications

authors： Takeda K,Oida S,Ichijo H,Iimura T,Maruoka Y,Amagasa T,Sasaki S

更新日期：1994-10-14 00:00:00

abstract：:The kallikrein inhibitor found in Bauhinia bauhinioides seeds (BbKI) differs from classical Kunitz plant inhibitors in the lack of disulfide bridges in its structure [Biochim. Biophys. Acta 1477 (2000) 64-74]. In this study, we examined whether structural properties may be involved in inhibitory specificity and, if so...

journal_title：Biochemical and biophysical research communications

authors： Cagliari CI,De Caroli FP,Nakahata AM,Araújo MS,Nakaie CR,Sampaio MU,Sampaio CA,Oliva ML

更新日期：2003-11-07 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19 (coronavirus disease-19), represents a far more serious threat to public health than SARS and MERS coronaviruses, due to its ability to spread more efficiently than its predecessors. Currently, there is no worldwide-approved ...

journal_title：Biochemical and biophysical research communications

authors： Santopolo S,Riccio A,Santoro MG

更新日期：2020-11-28 00:00:00

abstract：:Apoptosis induced in primary cultures of rat hepatocytes by transforming growth factor beta 1 (TGF-beta 1) was greatly attenuated by inhibitors (5 microM) of calpain I and calpain II, respectively. Both inhibitors prevented the TGF-beta-elicited increase of nucleosomal DNA fragments and the occurrence of DNA-breaks in...

journal_title：Biochemical and biophysical research communications

authors： Gressner AM,Lahme B,Roth S

更新日期：1997-02-13 00:00:00

abstract：:By its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate ...

journal_title：Biochemical and biophysical research communications

authors： Kajiya M,Silva MJ,Sato K,Ouhara K,Kawai T

更新日期：2009-08-14 00:00:00

abstract：:Previous reports have shown that 12-0-tetradecanoylphorbol-13-acetate can activate phospholipase D in human peripheral blood mononuclear cells as measured by an enzyme-catalyzed transphosphatidylation reaction (phosphatidylethanol formation). In the present study, the mononuclear cells were fractionated by two procedu...

journal_title：Biochemical and biophysical research communications

authors： Kinsky SC,Loader JE,Benedict SH

更新日期：1989-07-31 00:00:00

abstract：:Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coi...

journal_title：Biochemical and biophysical research communications

authors： Martherus RS,Sluiter W,Timmer ED,VanHerle SJ,Smeets HJ,Ayoubi TA

更新日期：2010-11-12 00:00:00

abstract：:In rice, Hd3a, GF14 and OsFD1 proteins, forming florigen activation complex, are key components in flowering time regulation. GF14 genes in rice response to biotic and abiotic stress has also been well addressed. The role of GF14e in rice defense has been well studied. However, whether Hd3a and OsFD1 play roles in def...

journal_title：Biochemical and biophysical research communications

authors： Ke Y,Wu M,Zhang Q,Li X,Xiao J,Wang S

更新日期：2019-06-11 00:00:00

abstract：:Human platelets contained proteins which cross-reacted with antisera specific for brain protein kinase C-alpha and -beta. When platelets were incubated with 12-O-tetradecanoylphorbol-13-acetate there was a rapid accumulation of protein kinase C-alpha in the particulate fraction associated with a loss of this subspecie...

journal_title：Biochemical and biophysical research communications

authors： Fournier A,Hardy SJ,Clark KJ,Murray AW

更新日期：1989-06-15 00:00:00

abstract：:To study cellular and subcellular localization of TIMP-1, we constructed a cDNA which would express a chimeric protein, TIMP-1-EGFP, having the enhanced green fluorescent protein of the jelly fish Aequorea victoria fused to the carboxyl-terminus of TIMP-1. Chinese Hamster Ovary (CHO) cells were stably transfected with...

journal_title：Biochemical and biophysical research communications

authors： Ritter LM,Garfield SH,Thorgeirsson UP

更新日期：1999-04-13 00:00:00

abstract：:The products of the TSC1 (hamartin) and TCS2 (tuberin) tumor suppressor genes negatively regulate cell growth by inhibiting mTOR signaling. Recent research has led to the postulation that tuberin and/or hamartin are involved in tumor migration, presumably through Rho activation. Here we show that LEF-8 cells, which co...

journal_title：Biochemical and biophysical research communications

authors： Suzuki T,Das SK,Inoue H,Kazami M,Hino O,Kobayashi T,Yeung RS,Kobayashi K,Tadokoro T,Yamamoto Y

更新日期：2008-03-28 00:00:00

abstract：:Using small interfering RNA (siRNA) to induce sequence specific gene silencing is fast becoming a standard tool in functional genomics. As siRNAs in some cases tolerate mismatches with the mRNA target, knockdown of genes other than the intended target could make results difficult to interpret. In an investigation of 3...

journal_title：Biochemical and biophysical research communications

authors： Snøve O Jr,Holen T

更新日期：2004-06-18 00:00:00

abstract：:As part of an effort to identify genes potentially involved in the Down Syndrome pathogenesis, in this paper we report the identification and characterization of a new human gene (named SH3BGRL), which shows a high homology to the SH3BGR gene, previously mapped to the Down Syndrome region of chromosome 21. The SH3BGRL...

journal_title：Biochemical and biophysical research communications

authors： Egeo A,Mazzocco M,Arrigo P,Vidal-Taboada JM,Oliva R,Pirola B,Giglio S,Rasore-Quartino A,Scartezzini P

更新日期：1998-06-18 00:00:00

abstract：OBJECTIVE:Chondrocytes express many kinds of hormone receptors. The function of Follicle stimulating hormone (FSH) in the ovary is mediated by FSH receptor (FSHR). FSH receptor (FSHR) is found in many non-ovarian tissues, however it has been unclear if chondrocytes express FSHR. The purpose of this study is to determin...

journal_title：Biochemical and biophysical research communications

authors： Kong D,Guan Q,Li G,Xin W,Qi X,Guo Y,Zhao J,Xu J,Sun S,Gao L

更新日期：2018-01-01 00:00:00

abstract：:Regulation of phospholipase D (PLD) activation by protein kinase C (PKC) was studied in membranes isolated from human promyelocytic leukemia HL60 cells. The activation of membrane-bound PLD by PKC partially purified from rat brain was most effectively induced with phorbol 12-myristate 13-acetate (PMA) and Ca2+ (1 micr...

journal_title：Biochemical and biophysical research communications

authors： Ohguchi K,Banno Y,Nakashima S,Nozawa Y

更新日期：1995-06-06 00:00:00

abstract：:Kaposi sarcoma and primary effusion lymphoma cells are infected with Kaposi sarcoma-associated herpesvirus (KSHV), predominantly in the latent form, and KSHV replication is observed rarely. Angiogenesis plays a crucial role in the pathogenesis of both Kaposi sarcoma and primary effusion lymphoma. In this study, we fou...

journal_title：Biochemical and biophysical research communications

authors： Kanno T,Uehara T,Osawa M,Fukumoto H,Mine S,Ueda K,Hasegawa H,Katano H

更新日期：2015-08-07 00:00:00

abstract：:The involvement of Na+ in insulin-secretory responses to metabolizable and nonmetabolizable amino acids known to be cotransported with Na+, were examined using islet-derived BRIN-BD11 cells. At stimulatory (16.7 mM) glucose, 10 mM of l-alanine, alpha-aminoisobutyric acid (AIB) or l-proline stimulated 1.3- to 10. 4-fol...

journal_title：Biochemical and biophysical research communications

authors： McClenaghan NH,Barnett CR,Flatt PR

更新日期：1998-08-19 00:00:00

abstract：:Lipoprotein lipase (LPL) and angiopoietin-like protein 4 (Angptl4) were studied in 3T3-L1 adipocytes. Transfections of the adipocytes with Angptl4 esiRNA caused reduction of the expression of Angptl4 to about one fourth of that in cells treated with vehicle only. This resulted in higher levels of LPL activity both on ...

journal_title：Biochemical and biophysical research communications

authors： Makoveichuk E,Vorrsjö E,Olivecrona T,Olivecrona G

更新日期：2013-11-29 00:00:00

abstract：:The levels of the anti-aging protein α-Klotho, in its soluble form (s-Klotho), are depressed in the circulation of patients with type 1 diabetes (T1D) or type 2 diabetes (T2D). Gene transfer experiments have suggested a protective role for β-cell specific expression of α-Klotho in murine models of T1D and T1D, but the...

journal_title：Biochemical and biophysical research communications

authors： Prud'homme GJ,Glinka Y,Kurt M,Liu W,Wang Q

更新日期：2020-05-07 00:00:00

abstract：:Ambroxol is often used as a mucolytic agent in various lung diseases. However, it is unclear how ambroxol acts on bronchial epithelial cells. To clarify the action of ambroxol, we studied the effects of ambroxol on the ion transport in human Calu-3 cells, a human submucosal serous cell line, measuring the transepithel...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa I,Niisato N,Iwasaki Y,Marunaka Y

更新日期：2006-05-05 00:00:00