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PRaTo: a web-tool to select optimal primer pairs for qPCR.

Abstract：

:An essential pre-requisite to perform sound quantitative real-time polymerase chain reaction (qPCR) assays is to design outstanding primer pairs. This means they must have a good efficiency and be not prone to produce multiple amplicons or primer dimer products. To circumvent these issues, several softwares are available to help primer design. Although satisfactory computer-aided primer design tools are available for standard PCR, less efforts were done to provide specific methods for selection of optimal primer pairs for qPCR. We have developed PRaTo a web-based tool that enables checking and ranking of primers pairs for their attitude to perform optimally and reliably when used in qPCR experiments. PRaTo is available at http://prato.daapv.unipd.it.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Nonis A,Scortegagna M,Nonis A,Ruperti B

doi

10.1016/j.bbrc.2011.10.148

subject

Has Abstract

pub_date

2011-12-02 00:00:00

pages

707-8

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)01982-6

journal_volume

415

pub_type

杂志文章

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