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Participation of macrophage membrane rafts in Trypanosoma cruzi invasion process.

Abstract：

:Membrane rafts are small and dynamic regions enriched in sphingolipids, cholesterol, ganglioside GM1 and protein markers like flotillins, forming the flatter domains or caveolins, which are characterized as stable flask-shape invaginations. We explored whether membrane rafts participate in the entry of Trypanosoma cruzi's trypomastigotes into murine macrophages through transient depletion of macrophage membrane cholesterol with methyl-beta-cyclodextrin and treatment with filipin. These treatments led to a decrease in the trypomastigote invasion process. Macrophage pre incubated with increasing concentrations of cholera toxin B, that binds GM1, inhibited the adhesion and invasion of trypomastigote and amastigote forms. Immunofluorescence analysis demonstrated a colocalization of GM1, flotillin 1 and caveolin 1 in the T. cruzi parasitophorous vacuole. Taken together these data suggest that membrane rafts, including caveolae, are involved in the process of T. cruzi invasion of macrophages.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Barrias ES,Dutra JM,De Souza W,Carvalho TM

doi

10.1016/j.bbrc.2007.09.068

subject

Has Abstract

pub_date

2007-11-23 00:00:00

pages

828-34

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)02030-X

journal_volume

363

pub_type

杂志文章

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