Joint cultivation of human erythroblastoid cells and mouse fibroblasts triggers release of a wide spectrum of cytotoxic factors.

abstract：:A number of viruses, when they bind to cells, activate intracellular signals that facilitate post-binding steps of infection. To determine if retroviruses activate intracellular signaling, we transduced HeLa cells with amphotropic retroviruses produced by TelCeB6 cells and examined cell lysates for activated Rac1. We ...

journal_title：Biochemical and biophysical research communications

authors： Krishna D,Le Doux JM

更新日期：2006-07-07 00:00:00

abstract：:To investigate involvement of miRNAs in radiation responses we used microRNAome profiling to analyze the sex-specific response of radiation sensitive hematopoietic lymphoid tissues. We show that radiation exposure resulted in a significant and sex-specific deregulation of microRNA expression in murine spleen and thymu...

journal_title：Biochemical and biophysical research communications

authors： Ilnytskyy Y,Zemp FJ,Koturbash I,Kovalchuk O

更新日期：2008-12-05 00:00:00

abstract：:Most fish protamines contain two phosphorylatable sites both of which incorporate phosphate in vivo. Here we show that in two protamines (salmine A1 and clupeine Y1) the site more distant from the N-terminus (residues 20-21) is unaffected by cAMP-dependent protein kinase while it represents the main target for protein...

journal_title：Biochemical and biophysical research communications

authors： Ferrari S,Pinna LA

更新日期：1987-05-14 00:00:00

abstract：:Assay systems for the evaluation of carcinogen interaction with human tissues are essential for assessing cancer risk. Human hair roots (HHR) are a readily obtainable epithelial tissue source that have been employed for investigating inherited enzyme activities. In this study HHR were found to possess cytochrome P-450...

journal_title：Biochemical and biophysical research communications

authors： Merk HF,Mukhtar H,Schutte B,Kaufmann I,Das M,Bickers DR

更新日期：1987-10-29 00:00:00

abstract：:T cell immunotherapies are promising options in leukemia, among which the CD19/CD3-bispecific T cell engager antibody blinatumomab (MT103) has shown high response rates at very low doses in patients with lymphoma. However, the high CD47 expression in human lymphoma cells has limited the curative effects of blinatumoma...

journal_title：Biochemical and biophysical research communications

authors： Xu L,Wang S,Li J,Li B

更新日期：2019-02-12 00:00:00

abstract：:Lysosomal integral membrane protein-2 (LIMP-2) is a type III transmembrane protein that is highly glycosylated and mainly localized to the lysosomal membrane. The diverse functions of LIMP-2 are currently being uncovered; however, its participation in macroautophagy, usually described as autophagy, has not yet been we...

journal_title：Biochemical and biophysical research communications

authors： Sakane H,Urabe J,Nakahira S,Hino K,Miyata N,Akasaki K

更新日期：2020-12-17 00:00:00

abstract：:To evaluate the immunomodulatory effects of histamine in vivo, we analyzed an experimental syngenic tumor model using a colon adenocarcinoma cell line, CT-26, in Balb/c mice. In this model, distinct tumor growth was observed around 6 days after inoculation. Daily administration of cimetidine (0.12 mg/kg/day) significa...

journal_title：Biochemical and biophysical research communications

authors： Takahashi K,Tanaka S,Ichikawa A

更新日期：2001-03-01 00:00:00

abstract：:Protein synthesis and degradation balance have a crucial role in maintenance of cellular homeostasis and function. The ubiquitin-proteasome system is one of the major cellular proteolytic machineries responsible for the removal of normal, abnormal, denatured or in general damaged proteins. Proteasome is a multisubunit...

journal_title：Biochemical and biophysical research communications

authors： Sakellari M,Chondrogianni N,Gonos ES

更新日期：2019-06-18 00:00:00

abstract：:The expansion of a CAG trinucleotide repeat (TNR) sequence has been linked to several neurological disorders, for example, Huntington's disease (HD). In HD, healthy individuals have 5-35 CAG repeats. Those with 36-39 repeats have the premutation allele, which is known to be prone to expansion. In the disease state, gr...

journal_title：Biochemical and biophysical research communications

authors： Jarem DA,Delaney S

更新日期：2011-12-09 00:00:00

abstract：:A voltage-activated proton current in human cardiac fibroblasts, measured using the whole-cell recording configuration of the patch-clamp technique, is reported. Increasing the pH of the bathing solution shifted the current activation threshold to more negative potentials and increased both the current amplitude and i...

journal_title：Biochemical and biophysical research communications

authors： El Chemaly A,Guinamard R,Demion M,Fares N,Jebara V,Faivre JF,Bois P

更新日期：2006-02-10 00:00:00

abstract：:Ambient cold temperature, as an abiotic stress, regulates the survival, stability, transmission, and infection of pathogens. However, the effect of cold temperature on the host receptivity to the pathogens has not been fully studied. In this study, the expression of terminal α-2,3- and α-2,6-sialic acids were increase...

journal_title：Biochemical and biophysical research communications

authors： Moon SC,Joo SY,Chung TW,Choi HJ,Park MJ,Choi HJ,Bae SJ,Kim KJ,Kim CH,Joo M,Ha KT

更新日期：2016-07-29 00:00:00

abstract：:The effects of arachidonic acid on the enzyme complexes in the electron transport system were investigated using submitochondrial particles from rat brain. Arachidonic acid irreversibly inhibited NADH-CoQ oxidoreductase (complex I) activity, but had no effect on the activities of succinate-CoQ oxidoreductase (complex ...

journal_title：Biochemical and biophysical research communications

authors： Morii H,Takeuchi Y,Watanabe Y

更新日期：1991-08-15 00:00:00

abstract：:A monoclonal antibody (MaB) against mouse sarcoma DNA polymerase alpha was isolated from the culture medium of an IgG-secreting hybridoma. The MaB demonstrated reactivity against two murine DNA polymerase alpha preparations and a calf thymus DNA polymerase alpha. Murine sarcoma polymerase was activated in vitro by pho...

journal_title：Biochemical and biophysical research communications

authors： Sylvia VL,Norman JO,Curtin GM,Busbee DL

更新日期：1986-11-26 00:00:00

abstract：:Polyclonal antibodies (pAbs) to riboflavin carrier protein (RCP) purified from human amniotic fluid were raised in two rabbits. These pAbs apart from recognizing the parent antigen, reacted significantly with chicken RCP as well. When all the 212 octapeptides overlapping by one amino acid residue and covering the enti...

journal_title：Biochemical and biophysical research communications

authors： Subramanian S,Adiga PR

更新日期：1999-08-27 00:00:00

abstract：:There is increasing evidence that a functional interaction exists between interleukin-1beta (IL-1beta) and N-methyl-D-aspartate (NMDA) receptors. The present study attempted to elucidate the effect of IL-1beta on the NMDA-induced outward currents in mechanically dissociated hippocampal neurons using a perforated patch...

journal_title：Biochemical and biophysical research communications

authors： Zhang R,Yamada J,Hayashi Y,Wu Z,Koyama S,Nakanishi H

更新日期：2008-08-08 00:00:00

abstract：:The relationship between vascular tone and the induction by endotoxin of a nitric oxide (NO) synthase was studied in vitro in rings of rat thoracic aorta. In rings with and without endothelium there was a time-dependent induction of NO synthase accompanied by both spontaneous and L-arginine-induced relaxation and by r...

journal_title：Biochemical and biophysical research communications

authors： Rees DD,Cellek S,Palmer RM,Moncada S

更新日期：1990-12-14 00:00:00

abstract：:Histone lysine N-methyltransferase 2D (KMT2D), an important methyltransferase that is involved in the methylation of lysine 4 in histone H3 (H3K4) and related to the development of prostate cancer. Hypermethylation of H3K4 is shown in prostate cancer (PCa). However, KMT2D inhibitors have not yet been developed. This a...

journal_title：Biochemical and biophysical research communications

authors： Yu Q,Liao Z,Liu D,Xie W,Liu Z,Liao G,Wang C

更新日期：2020-12-10 00:00:00

abstract：:Helodermin is a peptide from the venom of the lizard Heloderma suspectum (Gila Monster) showing a high degree of sequence similarity with VIP, PHI and secretin in its N-terminal moiety. The present data support the presence of peptide(s) closely related to helodermin in the brain, gut and salivary glands of rat. In ou...

journal_title：Biochemical and biophysical research communications

authors： Robberecht P,De Graef J,Woussen MC,Vandermeers-Piret MC,Vandermeers A,De Neef P,Cauvin A,Yanaihara C,Yanaihara N,Christophe J

更新日期：1985-07-16 00:00:00

abstract：:The extravascular localization of tissue factor (TF), the central initiator of coagulation, is thought to ensure that thrombus formation is prevented in the intact vessel. We observed that during a 5-min stimulation of human blood with collagen (type I), TF antigen appeared on the surface of platelets adhering to leuk...

journal_title：Biochemical and biophysical research communications

authors： Zillmann A,Luther T,Müller I,Kotzsch M,Spannagl M,Kauke T,Oelschlägel U,Zahler S,Engelmann B

更新日期：2001-02-23 00:00:00

abstract：:Chlorpromazine is a potent inhibitor of calmodulin and can alter the accumulation, metabolism, and incorporation into RNA of several fluoropyrimidine drugs. Using S-180 murine sarcoma cells, the accumulation of the base Fluorouracil and its metabolism to ribonucleotides is enhanced by chlorpromazine treatment, as meas...

journal_title：Biochemical and biophysical research communications

authors： Tseng A Jr,Brooks M,Cadman E

更新日期：1986-07-31 00:00:00

abstract：:Modern proteomics approaches include techniques to examine the expression, localization, modifications, and complex formation of proteins in cells. In order to address issues of protein function in vitro using classical biochemical and biophysical approaches, high-throughput methods of cloning the appropriate reading ...

journal_title：Biochemical and biophysical research communications

authors： Huang RY,Boulton SJ,Vidal M,Almo SC,Bresnick AR,Chance MR

更新日期：2003-08-08 00:00:00

abstract：:Using recombinant DNA technology, the NH2 and COOH terminal domains of the human Factor VIII molecule were co-expressed in baby hamster kidney 21 (BHK21) cells using the vaccinia virus system. Procoagulant activity was detectable in cell supernatants, thus suggesting that the central portion present in the FVIII prote...

journal_title：Biochemical and biophysical research communications

authors： Pavirani A,Meulien P,Harrer H,Dott K,Mischler F,Wiesel ML,Mazurier C,Cazenave JP,Lecocq JP

更新日期：1987-05-29 00:00:00

abstract：:A voltage-dependent K+ current has been revealed in whole-cell recordings carried out on immortalized cells obtained from the human tracheal epithelium. At positive membrane potentials the current shows a time dependent inactivation which is accelerated by increasing the depolarizing step. Forskolin, a direct activato...

journal_title：Biochemical and biophysical research communications

authors： Galietta LJ,Rasola A,Barone V,Gruenert DC,Romeo G

更新日期：1991-09-30 00:00:00

abstract：:Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear DNA binding protein that participates in processes involving nicking and resealing DNA strands. A genomically unstable subpopulation of PARP-1(-/-) cells has recently been described, which disappears after stable transfection of the cells with complete PARP-1 cDNA. H...

journal_title：Biochemical and biophysical research communications

authors： Cayuela ML,Carrillo A,Ramirez P,Parrilla P,Yélamos J

更新日期：2001-07-13 00:00:00

abstract：:We previously demonstrated several nicotinic acetylcholine receptor (nAChR) subunits and associated proteins in human sperm. Here, we identified in sperm for the first time two additional nAChR-associated molecules: (1) agrin(SN)Z(+) in human sperm localized in the posterior post-acrosomal, neck, and flagellar mid-pie...

journal_title：Biochemical and biophysical research communications

authors： Kumar P,Ferns MJ,Meizel S

更新日期：2006-04-07 00:00:00

abstract：:Studies have shown that High mobility group A2 (HMGA2), a non-histone protein, can promote epithelial-mesenchymal transition (EMT), which plays a critical role in prostate cancer progression and metastasis. Interestingly, full-length or wild-type HMGA2 and truncated (lacking the 3'UTR) HMGA2 isoforms are overexpressed...

journal_title：Biochemical and biophysical research communications

authors： Hawsawi O,Henderson V,Burton LJ,Dougan J,Nagappan P,Odero-Marah V

更新日期：2018-09-26 00:00:00

abstract：:Cellular defects which prevent apoptotic cell death can result in the generation of hyperproliferative disorders and can prevent the effective treatment of such diseases. The majority of cellular defects which result in apoptosis resistance lie upstream of caspase activation. We have described chimeric caspase molecul...

journal_title：Biochemical and biophysical research communications

authors： Shearwin-Whyatt L,Baliga B,Doumanis J,Kumar S

更新日期：2001-04-20 00:00:00

abstract：:To overcome the limitations of existing models, we developed a novel experimental in vivo platform for replacing mouse liver with functioning human liver tissue. To do this, a herpes simplex virus type 1 thymidine kinase (HSVtk) transgene was expressed within the liver of highly immunodeficient NOG mice (TK-NOG). Mous...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa M,Kawai K,Mitsui T,Taniguchi K,Monnai M,Wakui M,Ito M,Suematsu M,Peltz G,Nakamura M,Suemizu H

更新日期：2011-02-18 00:00:00

abstract：:Lipoteichoic acid is a major cell wall virulence factor of gram-positive bacteria. LTAs from various bacteria have differential immunostimulatory potentials due to heterogeneity in their structures. Although recent studies have demonstrated that LTA isolated from Lactobacillus plantarum (pLTA) has anti-inflammatory pr...

journal_title：Biochemical and biophysical research communications

authors： Jang KS,Baik JE,Han SH,Chung DK,Kim BG

更新日期：2011-04-22 00:00:00

abstract：:Binding of ATP, but not of ADP, activates Escherichia coli DnaA protein for replicational initiation of the chromosome. To elucidate this switching mechanism, we used the affinity-labeling agent ATP-pyridoxal, which forms a covalent bond with the Lys residue located at or near the gamma-phosphate of ATP. ATP-pyridoxal...

journal_title：Biochemical and biophysical research communications

authors： Kubota T,Ito Y,Sekimizu K,Tagaya M,Katayama T

更新日期：2001-11-16 00:00:00