A voltage-activated proton current in human cardiac fibroblasts.

abstract：:The cis-regulatory region of target genes is key elements in the transcriptional regulation of gene expression. Many of these cis-regulatory regions have not been identified by either biological experiments or computational methods. Recently, a few additional C(2)H(2) zinc finger transcription factor binding sites hav...

journal_title：Biochemical and biophysical research communications

authors： Cho SY,Chung M,Park M,Park S,Lee YS

更新日期：2008-05-09 00:00:00

abstract：BACKGROUND:Simvastatin is widely used to lower cholesterol levels in patients with cardiovascular diseases, although accumulating evidence suggests that statins, such as simvastatin, also exert numerous anti-tumoral effects. AIM:The aim of this study was to examine the effect of simvastatin on colon cancer cell migrat...

journal_title：Biochemical and biophysical research communications

authors： Al-Haidari AA,Syk I,Thorlacius H

更新日期：2014-03-28 00:00:00

abstract：:Human platelets labeled with [3H]arachidonic acid and permeabilized with saponin produced [3H]1,2-diacylglycerol (DG) by phospholipase C and released [3H]arachidonate by phospholipase A2, when activated with thrombin. Thrombin-induced arachidonate liberation was almost completely inhibited with pretreatment of pertuss...

journal_title：Biochemical and biophysical research communications

authors： Nakashima S,Hattori H,Shirato L,Takenaka A,Nozawa Y

更新日期：1987-11-13 00:00:00

abstract：:Kaiso was previously described as a methylated DNA-binding protein and a transcription repressor interacting with the corepressor protein complex NCoR. In the current study, we show that generation-3 Kaiso knockout mice show a phenotype of splenomegaly and large diffused germinal centers (GC). In the spleens of Kaiso ...

journal_title：Biochemical and biophysical research communications

authors： Koh DI,Yoon JH,Kim MK,An H,Kim MY,Hur MW

更新日期：2013-12-13 00:00:00

abstract：:maf is a family of oncogenes, originally identified from the avian oncogenic retrovirus AS42, which encodes a nuclear bZip transcription factor protein. It has been reported that maf family genes have critical roles in embryological development and cellular differentiation. In this study, the distribution of maf-1 gen...

journal_title：Biochemical and biophysical research communications

authors： Imaki J,Onodera H,Tsuchiya K,Imaki T,Mochizuki T,Mishima T,Yamashita K,Yoshida K,Sakai M

更新日期：2000-06-16 00:00:00

abstract：:Intracellular free calcium ([Ca2+]i) was measured in individual pancreatic beta-cells from mice using dual emission microfluorometry and the indicator Indo-1 applied by a patch clamp pipette. GTP-gamma-S (100 microM) injected together with 0.3 or 3 mM ATP evoked repetitive [Ca2+]i transients with a frequency of about ...

journal_title：Biochemical and biophysical research communications

authors： Lund PE,Grapengiesser E,Gylfe E,Hellman B

更新日期：1991-06-14 00:00:00

abstract：:WNK kinases are novel serine/threonine protein kinases. Mutations in two members of the WNK family, WNK1 and WNK4, cause familial hyperkalemic hypertension. These kinases regulate ion transport across diverse epithelia; WNK4 reduces activity of the Na-Cl cotransporter activity and the potassium channel, ROMK, by reduc...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Yang CL,Ellison DH

更新日期：2004-05-07 00:00:00

abstract：:The mechanism by which lithium (Li(+)) inhibits the protein kinase glycogen synthase kinase-3 (GSK-3) is unknown. Here, we demonstrate that Li(+) is a competitive inhibitor of GSK-3 with respect to magnesium (Mg(2+)), but not to substrate or ATP. This mode of inhibition is conserved between mammalian and Dictyostelium...

journal_title：Biochemical and biophysical research communications

authors： Ryves WJ,Harwood AJ

更新日期：2001-01-26 00:00:00

abstract：:Leukotriene B(4) (LTB(4)) is a potent chemoattractant and activator of neutrophils, macrophages and T cells. These cells are a key component of inflammation and all express BLT1, a high affinity G-protein-coupled receptor for LTB(4). However, little is known about the neuroimmune functions of BLT1. In this study, we d...

journal_title：Biochemical and biophysical research communications

authors： Kihara Y,Yokomizo T,Kunita A,Morishita Y,Fukayama M,Ishii S,Shimizu T

更新日期：2010-04-09 00:00:00

abstract：:The bacterial superantigen staphylococcal enterotoxin A (SEA) is a potent inducer of CTL activity and cytokine production in vivo. Protein A (PA) of Staphylococcal aureus has been found to have diverse biological response modifying properties and to possess antitumor, antitoxic and antiparasitic effects. In this study...

journal_title：Biochemical and biophysical research communications

authors： Mondal TK,Bhatta D,Biswas S,Pal P

更新日期：2002-02-01 00:00:00

abstract：:A sample of highly oriented Na-hyaluronate was prepared with a wet--spinning method and equilibrated to 75% relative humidity. Its proton NMR spectrum was recorded as a function of the angle theta between the hyaluronate helix axis and the external magnetic field. It appeared as a singlet with an angle-dependent line ...

journal_title：Biochemical and biophysical research communications

authors： Lahajnar G,Rupprecht A

更新日期：1986-11-26 00:00:00

abstract：:Two genomic DNA clones encoding part of the mouse epidermal growth factor (EGF) receptor were isolated: an 8.0 kb insert that hybridized with the human EGF receptor kinase domain; and a 2.4 kb insert that mapped between the kinase and 3' autophosphorylation domains of the human EGF receptor gene. The 2.4 kb insert was...

journal_title：Biochemical and biophysical research communications

authors： Hung MC,Thompson KL,Chiu IM,Rosner MR

更新日期：1986-12-30 00:00:00

abstract：:A Ser48 phospholipase A2-homologue, ammodytin L, which is myotoxic in mammals and devoid of any phospholipase A2 activity, completely inhibits the specific binding of the neurotoxic phospholipase A2, ammodytoxin C, to fish presynaptic membranes from Torpedo marmorata electric organ. In cross-linking experiments, 125I-...

journal_title：Biochemical and biophysical research communications

authors： Pungercar J,Vucemilo N,Faure G,Bon C,Verheij HM,Gubensek F,Krizaj I

更新日期：1998-03-17 00:00:00

abstract：:The gene encoding the human puromycin-sensitive aminopeptidase (PSA) has been cloned and characterized. The human PSA gene is composed of 23 exons and 22 introns and spans approximately 40 kb of chromosome 17 at the interval 17q12-21. An analysis of the 5' end of the human PSA transcript reveals that the translational...

journal_title：Biochemical and biophysical research communications

authors： Thompson MW,Tobler A,Fontana A,Hersh LB

更新日期：1999-05-10 00:00:00

abstract：:Activated botulinum D toxin ADP-ribosylates a 22 kDa molecular weight protein in homogenates obtained by sonication of a suspension of rabbit peritoneal neutrophils. The ADP-ribosylation catalyzed by activated botulinum D toxin is inhibited in homogenates obtained from cells pretreated with the toxin, suggesting that ...

journal_title：Biochemical and biophysical research communications

authors： Mege JL,Volpi M,Becker EL,Sha'afi RI

更新日期：1988-04-29 00:00:00

abstract：:The enzymatic mechanism of a small ribosome-inactivating protein, gamma-momorcharin, purified from the seeds of Momordica charantia, has been characterized. By SDS-polyacrylamide and electrospray ionization mass spectrometry, its molecular weight was measured to be 11,500 daltons which is much lower than other RIPs kn...

journal_title：Biochemical and biophysical research communications

authors： Pu Z,Lu BY,Liu WY,Jin SW

更新日期：1996-12-04 00:00:00

abstract：:The acyclic nucleotide analogue (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(S)-HPMPA] is a potent and selective inhibitor of African swine fever virus (ASFV) replication. Using the DNA-DNA hybridization technique with plasmid pRPEL-2 as probe, we have shown that (S)-HPMPA exerts a specific, dose-dependent, in...

journal_title：Biochemical and biophysical research communications

authors： Arzuza O,García-Villalón D,Tabarés E,Gil-Fernández C,De Clercq E

更新日期：1988-07-15 00:00:00

abstract：:The effects of intracisternal (ic) injection of recombinant interleukin-1 beta (IL-1) on absolute ethanol-induced gastric necrotic lesions were studied in conscious rats. IL-1 given ic inhibited ethanol-induced gastric lesions. The cytoprotective effect was dose dependent (ED175 ng/rat), long lasting with a maximal ac...

journal_title：Biochemical and biophysical research communications

authors： Robert A,Saperas E,Zhang WR,Olafsson AS,Lancaster C,Tracey DE,Chosay JG,Taché Y

更新日期：1991-02-14 00:00:00

abstract：:Free fatty acids (FFAs) act as extracellular signaling molecules through binding to G-protein-coupled FFA receptors (FFARs). GPR120 and GPR40 are identified as FFARs for medium- and long-chain fatty acids. In the present study, we investigated roles of GPR120 and GPR40 in cellular functions of pancreatic cancer PANC-1...

journal_title：Biochemical and biophysical research communications

authors： Fukushima K,Yamasaki E,Ishii S,Tomimatsu A,Takahashi K,Hirane M,Fukushima N,Honoki K,Tsujiuchi T

更新日期：2015-09-25 00:00:00

abstract：:GOTO cells, a neuroblastoma cell line retaining the ability to differentiate into neuronal or Schwann cells, were found to be rich in membrane rafts containing ganglioside GM2 and hypersensitive to lipid raft-disrupting methyl-beta-cyclodextrin (MbetaCD); the GM2-rich rafts and sensitivity to MbetaCD were markedly dim...

journal_title：Biochemical and biophysical research communications

authors： Tomioka R,Minami N,Kushida A,Horibe S,Izumi I,Kato A,Fukushima K,Ideo H,Yamashita K,Hirose S,Saito Y

更新日期：2009-11-06 00:00:00

abstract：:While there have been many single-molecule studies of kinesin-1, most have been done along microtubules purified from bovine or porcine brain, and relatively little is known about how variations in tubulin might alter motor function. Of particular interest is transport along microtubules polymerized from tubulin purif...

journal_title：Biochemical and biophysical research communications

authors： Feizabadi MS,Jun Y

更新日期：2014-11-28 00:00:00

abstract：:Hemoglobin (Hb) isolated from the backswimmer Buenoa margaritacea has been analyzed spectroscopically. The met form at pH less than 6 shows a 30nm red shift in the Qv and Qo bands and a 5nm red shift in the Soret band compared to mammalian Hb, while only minor differences are seen in the spectra of the CO and O2 adduc...

journal_title：Biochemical and biophysical research communications

authors： Osmulski PA,Vossbrinck CR,Sampath V,Caughey WS,Debrunner PG

更新日期：1992-09-16 00:00:00

abstract：:Epigenetic gene silencing by aberrant DNA methylation leads to loss of key cellular pathways in tumorigenesis. DNA methylation-mediated silenced genes in pancreatic cancer were searched for using the methyl-CpG targeted transcriptional activation (MeTA) method, and LHX6 (LIM homeobox 6), a transcription factor involve...

journal_title：Biochemical and biophysical research communications

authors： Abudurexiti Y,Gu Z,Chakma K,Hata T,Motoi F,Unno M,Horii A,Fukushige S

更新日期：2020-06-04 00:00:00

abstract：:A growing body of evidence indicates that presenilins could exist and be active as oligomeric complexes. Using yeast two-hybrid and cell culture analysis, we provide evidence that presenilin-1 (PS1) may self-oligomerize giving rise to specific full-length/full-length homodimers. When expressed in N2A and HEK239T cultu...

journal_title：Biochemical and biophysical research communications

authors： Hébert SS,Godin C,Tomiyama T,Mori H,Lévesque G

更新日期：2003-01-31 00:00:00

abstract：:Genomic DNA analysis revealed that the coding region of the rat histamine H3 receptor comprises three exons interrupted by two introns of approximately 1 kb each. Several H3 receptor mRNA variants were identified by PCR and cDNA cloning and sequencing. Four variants generated by pseudo-intron retention/deletion at the...

journal_title：Biochemical and biophysical research communications

authors： Morisset S,Sasse A,Gbahou F,Héron A,Ligneau X,Tardivel-Lacombe J,Schwartz JC,Arrang JM

更新日期：2001-01-12 00:00:00

abstract：:Mitochondria dysfunction plays a significant role in the apoptosis of retinal cells. Diabetes activates retinal matrix metalloproteinases (MMP-9 and MMP-2), damages retinal mitochondria and activates the apoptotic machinery. This study is to investigate the temporal relationship between the activation of retinal MMPs ...

journal_title：Biochemical and biophysical research communications

authors： Santos JM,Tewari S,Lin JY,Kowluru RA

更新日期：2013-09-06 00:00:00

abstract：:Hepatitis A virus (HAV) protein 3AB is a membrane-interacting protein containing a stretch of 21 hydrophobic amino acid residues. The nature of its membrane association was studied in detail by analysing various deletion mutants. In vivo and in vitro expression of the wild-type protein and its mutants allowed to demon...

journal_title：Biochemical and biophysical research communications

authors： Ciervo A,Beneduce F,Morace G

更新日期：1998-08-10 00:00:00

abstract：:A tryptic peptide of bovine beta-casein (amino acid residues 184-202) was used as a model antigen to investigate how the relative position of the (helper) T cell and B cell determinants on a protein antigen influences the antibody response. Immunization with the peptide elicited a considerably higher anti-peptide resp...

journal_title：Biochemical and biophysical research communications

authors： Hachimura S,Enomoto A,Kaminogawa S

更新日期：1990-06-15 00:00:00

abstract：:Reactive oxygen species (ROS) are an important factor in the development of skin lesions in diabetes. A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (()OH) and peroxynitrite (ONOO(-)) in cell-free systems, whereas it seldom reacts with other ROS. Fibroblasts are a key component of skin. In t...

journal_title：Biochemical and biophysical research communications

authors： Yu P,Wang Z,Sun X,Chen X,Zeng S,Chen L,Li S

更新日期：2011-06-03 00:00:00

abstract：:Cathepsin D, a lysosomal aspartic proteinase, has been shown to induce apoptosis of HeLa cells when overexpressed. To further understand regulatory mechanisms of cathepsin D-induced cell death, we examined whether lysosomal cysteine and aspartic proteinases are involved in apoptosis of PC12 cells following serum depri...

journal_title：Biochemical and biophysical research communications

authors： Shibata M,Kanamori S,Isahara K,Ohsawa Y,Konishi A,Kametaka S,Watanabe T,Ebisu S,Ishido K,Kominami E,Uchiyama Y

更新日期：1998-10-09 00:00:00